Evaluation of effects of 1,3-dinitrobenzene on sperm motility of hamster using computer assisted semen analysis (CASA)

作     者：L.D.C.Peiris H.D.M.Moore 

作者机构：Dept. of Zoology University of Colombo Colombo 03 Sri LankaDept. of Molecular Biology and Biotechnology University of Sheffield Sheffield S 10 2TN UK 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2001年第3卷第2期

页      面：109-114页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：mDNB sperm motility CASA sperm fertility 

摘      要：Aim: To evaluate the effects of 1,3-dinitrobenznene (mDNB) on sperm motility of hamster and to correlate the re-sults with the fertility. Methods: Adult male hamsters were gavaged with one of the 3 dose regimes of mDNB(1.5 mg daily for 4 weeks ,1.5 mg one day a week for 4 weeks and 1.0 mg 3 days a week for 4 weeks). Computerassisted semen analysis (CASA) was used to analyse the sperm motility parameters, curvilinear velocity (VCL) andstraight line velocity (VSL) of sperm in distal corpus epididymides and distal cauda epididymides. In vitro fertilisationwas carried out only for 1.5 mg mDNB daily group to determine the sperm fertilising capacity. Results: There wasa significant reduction in sperm velocity parameters at weeks 3 and 4 after treatment, which was correlated with a de-cline in sperm fertility. Conclusion; Sperm velocity parameters may be used to determine the effect of a toxic insulton the sperm function.