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Expression of p53,c-erbB-2 and Ki67 in intestinal metaplasia and gastric carcinoma

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World Journal of Gastroenterology 2010年第3期16卷 339-344页

作者： Zheng, Yao Wang, Lin Zhang, Jian-Ping Yang, Jing-Yan Zhao, Zhu-Mei Zhang, Xiao-Ying Shandong Univ Qilu Hosp Dept Pathol Jinan 250012 Shandong Peoples R China Shandong Univ Sch Med Dept Pathol Jinan 250012 Shandong Peoples R China

AIM:To compare two types of classification of intestinal metaplasia (IM) of the stomach and to explore their relationship to gastric ***:Forty-seven cases of gastric IM were classified into type or typeaccordingto mucin histochemical staining and compared with a novel classification in which the specimens were classif ied into simple IM (SIM) or atypical IM according to polymorphism in terms of atypical changes of the metaplastic epithelium. Forty-seven IM and thirty-seven gastric carcinoma samples were stained for p53, c-erbB-2 and Ki67 proteins by Envision immunohistochemical ***: There were no significant differences in the expression of p53 and c-erbB-2 among type type, typeIM and gastric carcinomas. The positive expression rate of Ki67 was significantly higher in gastric carcinomas than in type IM while no signif icant Ki67 expression differences were observed among type,typeIM and gastric carcinomas. The expression ofp53, c-erbB-2 and Ki67 proteins in 20 SIM, 27 Atypical IM and 37 gastric carcinomas showed significant differences between SIM and gastric carcinomas while no significant differences were observed between Atypical IM and gastric ***: Atypical IM may better reveal the pre-cancerous nature of IM and could be a helpful indicator in the clinical follow up of patients.

关键词： Simple intestinal metaplasia p53 Atypical intestinal metaplasia c-erbB-2 Ki67

Hepatic angiomyolipoma-misdiagnosis as hepatocellular carcinoma:A report of 14 cases

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World Journal of Gastroenterology 2000年第4期6卷 608-612页

作者： Zhong DR Ji XL Gen Hosp PLA Dept Pathol Beijing 100853 Peoples R China

Angiomyolipoma (AML) is a rare benign mesenchymal tumor of the liver, composed of a varying heterogeneous mixture of three tissue components: blood vessels, smooth muscle, and adipose cells. It has recently been proposed that the perivascular epithelial cell (PEC) is the common progenitor[1，2] Since its first description by Ishak in 1976[3], there have been more than 100 cases reported in the English literature[4-6]. With the advance of radiological techniques, many more tumors are being diagnosed by the means. But radiological findings of AML may only be suggestive of the lesion; its definitive diagnosis requires histological confirmation[9-19]. Some authors regard renal and hepatic AMLs, pulmonary and soft tissue lymphangiomyomatosis[2], pulmonary and pancreatic clear cell “sugar” tumor, and cardiac rhabdomyoma as closely related groups of tumors, based on their morphologic overlap and common immunoreactivity for HMB-45[l]. They show different microscopic appearances, however, according to their organ of origin. The goals of this study were to highlight more subtle morphology and to gain possible insights into the differential diagnosis that could provide important information about this disease.

关键词： angiomyolipoma liver neoplasms/diagnosis immunohistochemistry microscopy electron carcinoma hepatocellular/pathology case-control studies

pathological differential diagnosis of solid-pseudopapillary neoplasm and endocrine tumors of the pancreas

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World Journal of Gastroenterology 2010年第8期16卷 1025-1030页

作者： Liu, Bao-An Li, Zhuo-Ming Su, Zhan-San She, Xiao-Ling Cent S Univ Xiangya Basic Med Sch Dept Pathol Changsha 410013 Hunan Peoples R China Cent S Univ Xiangya Hosp 3 Dept Pathol Changsha 410013 Hunan Peoples R China Cent S Univ Xiangya Hosp 2 Dept Pathol Changsha 410006 Hunan Peoples R China

AIM:To investigate differential points of solid-pseudo-papillary neoplasm (SPN) of the pancreas and pancre-atic endocrine tumor (PET).METHODS:Ten cases of SPN and fourteen cases of PET were studied in this retrospective study. Clinical and pathologic features,immunostaining reactions and β-catenin gene mutations were ***:The mean age of SPN patients was 25.6 years and these patients had no specific symptoms. The mean diameter of the tumors was 11.0 cm,9/10 cases were cystic or a mixture of solid and cystic structures,and there was hemorrhage and necrosis on the cut surface in 8/10 (80%) cases. Characteristic pseudo-papillary structure and discohesive appearance of the neoplastic cells were observed in all 10 (100%) cases. The results of immunostaining showed that nuclear expression of β-catenin and loss of E-cadherin in all the cases,was only seen in SPN. Molecular studies discov-ered that 9/10 (90%) cases harbored a point mutation of exon 3 in β-catenin gene. On the other hand,the mean age of PET patients was 43.1 years. Eight of 14 cases presented with symptoms caused by hypoglyce-mia,and the other 6 cases presented with symptoms similar to those of SPN. The mean size of the tumors was 2.9 cm,most of the tumors were solid,only 3/14 (21%) were a mixture of solid and cystic structures,and macroscopic hemorrhage and necrosis were much less common (3/14,21%). Histologically,tumor cells were arranged in trabecular,acinar or solid patterns and demonstrated no pseudopapillary structure and discohesive appearance in all 14 (100%) cases. The results of immunostaining and mutation detection were completely different with SPN that membrane and cytoplastic expression of β-catenin without loss of E-cadherin,as well as no mutation in β-catenin gene in all the cases. CONCLUSION:Both macroscopic and microscopic features of SPN are quite characteristic. It is not difficult to distinguish it from PET. If necessary,immunos-taining of β-catenin and E-cadherin is quite he

关键词： Solid-pseudopapillary neoplasm of the pan-creas Pancreatic endocrine tumor Immunohistochem-istry β-catenin gene Differential diagnosis

Liver inflammatory pseudotumor or parasitic granuloma?

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World Journal of Gastroenterology 2000年第3期6卷 458-460页

作者： Ji XL Shen MS Yin T Gen Hosp Chinese PLA Dept Pathol Beijing 100853 Peoples R China

INTRODUCTION Liver pseudotumor is a very rare benign *** the first case reported by Pack and Baker in1953,only 40 cases had been reported up to *** diagnostic challenge of hepatic inflammatorypseudotumor is emphasized... 详细信息

关键词： inflammatory pseudotumor parasitic granuloma ascariasis larva liver disease

Methylation status of p16 gene in colorectal carcinoma and normal colonic mucosa

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World Journal of Gastroenterology 1999年第5期5卷 451-454页

作者： Zhang J Lai MD Chen J Hangzhou Railway Cent Hosp Dept Clin Pathol Hangzhou 310000 Zhejiang Provin Peoples R China Zhejiang Univ Sch Med Dept Pathol Hangzhou 310006 Zhejiang Provin Peoples R China

INTRODUCTIONp16 gene (also known as MTS-1, INK4a, CDKN2A), located on chromosome 9p21, is a G1-specific cell-cycle regulatory gene. It is composed of three exons, which encode 156 amino acids[1]. The gene is frequently inactivated in many human cancers. Unlike other tumor-suppressor genes that are commonly inactivated by point mutations, small homozygous deletions and methylation of the promoter represent the major mechanism of p16 gene inactivation[2,3]. In the Western countries, colorectal carcinoma ranks first among malignant tumors. The mortality from colorectal carcinoma has been rapidly growing in China in the last two to three decades. The genetic alterations involved in this tumor are still unclear. Rare mutation and infrequent deletion of p16 gene in primary colorectal carcinoma has been widely reported[4]. There were a few papers on p16 gene methylation in colorectal carcinoma, but the results were contradictory[5,6]. In this study, we have examined a total of 60 samples of colorectal carcinoma and paired 60 samples of the normal colonic mucosa for methylation by means of PCR-based methylation assay. There is infrequent methylation in the promoter of p16 gene both in colorectal carcinoma and normal colonic mucosa. The methylation status in 5′ CpG island of p16 gene in colorectal carcinoma is not related to the clinical pathologic parameters of these tumors.

关键词： Colonic mucosa colorectal neoplasms p16 gene methylation

An improvement method for the detection of insitu telomerase activity : in situ telomerase activity labeling

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World Journal of Gastroenterology 1999年第6期5卷 535-537页

作者： Feng DY Zheng H Fu CY Cheng RX Hunan Med Univ Dept Pathol Changsha 410078 Hunan Province Peoples R China

INTRODUCTIONTelomerase is a special reverse transcriptase which consists of a template RNA and protein, and uses the RNA template to catalyze the addition of telomeric DNA to chromosome ends[1-5]. The enzyme is active in adult male germ-line cells, embryonic cells and hemopoietic stems, but is undetectable in normal somatic cells except for proliferative cells of renewal tissue, e.g., activated lymphocytes, basal cells of the epidermis and intestinal crypt cells, whereas in almost all malignant tumor cells and immortal cell lines, telomerase activity has been measured. Thus, it is believed that activation of telomerase is closely associated with genesis and development of malignant tumors.

关键词： methodology telomerase in situ labeling polymerase chain reaction

Kupffer cell and apoptosis in experimental HCC

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World Journal of Gastroenterology 2000年第3期6卷 405-407页

作者： Zhu HZ Ruan YB Wu ZB Zhang CM Tongji Med Univ Dept Ultrastruct Pathol Wuhan 430030 Hubei Province Peoples R China

INTRODUCTION Our previous study has proved that Kupffer cellsmay have an inhibitory effect on the process ofhepatocarcinogenesis,however,their inhibitorymechanism needs exploring *** performed acomparative study on the expression of PCNA,Bax,P53 and apoptosis of liver cancer cells usingimmunohistochemical technology and terminaldeoxynucleotidyl transferase (TdT)-mediateddUTP-digoxigenin nick end labeling(TUNEL)

关键词： liver noplasms experimental diethylnitrosamine apoptosis protein P53 Kupffer’s cell

EZH2 and STAT6 expression profiles are correlated with colorectal cancer stage and prognosis

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World Journal of Gastroenterology 2010年第19期16卷 2421-2427页

作者： Wang, Cheng-Gang Ye, Ying-Jiang Yuan, Jing Liu, Fang-Fang Zhang, Hui Wang, Shan Beijing Univ Dept Surg Gastroenterol Surg Oncol Lab Peoples Hosp Beijing 100044 Peoples R China Chinese Peoples Liberat Army Hosp Dept Pathol Beijing 100036 Peoples R China Beijing Univ Peoples Hosp Dept Pathol Beijing 100044 Peoples R China

AIM: To investigate the role of enhancer of zeste homologue 2 (EZH2) and STAT6 immunohistochemistry in the evaluation of clinical stages and prognosis of colorectal cancer (CRC). METHODS: The expression patterns were examined by immunohistochemistry in both tumor and adjacent non-neoplastic tissues of 119 CRC patients who underwent operation during the time period from 2002 to 2004. RESULTS: The positive rates of EZH2 and STAT6 in CRC cases were 69.7% (83 of 119) and 60.5% (72 of 119), respectively, and there was signifi cant differ-ence when compared with tumor adjacent non-neoplastic tissues (P < 0.05). In all CRC cases, patientswith EZH2-positive, or STAT6-positive expression had lower survival rates than those with EZH2-negative or STAT6-negative expression (P = 0.002 and P = 0.005, respectively). Co-expression of EZH2 and STAT6 showed signifi cantly higher levels in CRC cases of high clinical TNM stages (P = 0.001), and the expression of STAT6 was also correlated with lymph node metastasis and distant metastasis (P = 0.001 and P = 0.016, respectively). Multivariate analysis revealed that EZH2 expression was an independent prognostic indicator of CRC (P = 0.039). CONCLUSION: EZH2 and STAT6 expressions have significant values in distinguishing clinical stages of CRC and predicting the prognosis of the patients.

关键词： Colorectal Neoplasms Enhancer of zeste homologue 2 STAT6 Immunohistochemistry

Clinicopathological features and prognosis assessment of extranodal follicular dendritic cell sarcoma

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World Journal of Gastroenterology 2010年第20期16卷 2504-2519页

作者： Li, Lan Shi, Yong-Hong Guo, Zhi-Juan Qiu, Tian Guo, Lei Yang, Hong-Ying Zhang, Xun Zhao, Xin-Ming Su, Qin Chinese Acad Med Sci Inst Canc Dept Pathol Beijing 100021 Peoples R China Chinese Acad Med Sci Canc Hosp Dept Pathol Beijing 100021 Peoples R China Peking Union Med Coll Beijing 100021 Peoples R China Inner Mongolia Med Coll Affiliated Hosp 1 Dept Pathol Hohhot 010059 Inner Mongolia Peoples R China Inner Mongolia Med Coll Renmin Hosp Dept Pathol Hohhot 010059 Inner Mongolia Peoples R China Chinese Acad Med Sci Inst Canc Dept Radiol Beijing 100021 Peoples R China Chinese Acad Med Sci Canc Hosp Dept Radiol Beijing 100021 Peoples R China

AIM: To establish a model for prognosis assessment of extranodal follicular dendritic cell (FDC) ***: Nine lesions were examined by routine and molecular *** factors from the new cases and 97 reported cases were analyzed for their prognostic ***: The current lesions were found in f ive male and four female patients,located mainly in the head and neck area and averaging 7.2 cm in *** patients had recurrence or metastasis and three remained free of *** 106 patients (male/female ratio,1.1:1) were aged from 9 to 82 years (median,44 years).The tumor sizes ranged from 1.5 to 21 cm (mean,7.4 cm).Abdominal/pelvic region was affected most frequently (43%).Surgical resection was performed in 100 patients,followed by radiation and/or chemotherapy in 35 of ***-up data were available in 91 cases,covering a period of 3-324 mo (mean,27 mo;median,19 mo).Of the informative cases,38 (42%) had recurrence or metastasis,and 12 (13%) died of the *** tumors were classif ied histologically into lowand high-grade lesions.A size ≥ 5 cm (P = 0.003),highgrade histology (P = 0.046) and a mitotic count ≥ 5/10 HPF (P = 0.013) were associated with tumor *** lesions were def ined as low-,intermediateand high-risk tumors,and their recurrence rates were 16%,46% and 73%,and their mortality rates 0%,4% and 45%,***: Extranodal FDC tumors behave like soft tissue *** clinical outcomes are variable and can be evaluated according to their sizes and grades.

关键词： Extranodal follicular dendritic cell sarcoma Prognosis assessment Histologic grade Immunohistochemistry In situ hybridization Mutation detection