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Gene expression profiling of MYC-driven tumor signatures in porcine liver stem cells by transcriptome sequencing

World Journal of Gastroenterology 2015年第7期21卷 2011-2029页

作者： Rajagopal N Aravalli Neil C Talbot Clifford J Steer Department of Radiology University of Minnesota Medical School Minneapolis Beltsville Agricultural Research Center US Department of Agriculture departments of Medicine and Genetics Cell Biology and Development University of Minnesota

AIM:To identify the genes induced and regulated by the MYC protein in generating tumors from liver stem ***:In this study,we have used an immortal porcine liver stem cell line,PICM-19,to study the role of c-MYC in ***-19 cells were converted into cancer cells(PICM-19-CSCs)by overexpressing human *** identify MYC-driven differential gene expression,transcriptome sequencing was carried out by RNA sequencing,and genes identified by this method were validated using real-time *** vivo tumorigenicity studies were then conducted by injecting PICM-19-CSCs into the flanks of immunodeficient ***:Our results showed that MYC-overexpressing PICM-19 stem cells formed tumors in immunodeficient mice demonstrating that a single oncogene was sufficient to convert them into cancer cells(PICM-19-CSCs).By using comparative bioinformatics analyses,we have determined that>1000 genes were differentially expressed between PICM-19 and *** ontology analysis further showed that the MYCinduced,altered gene expression was primarily associated with various cellular processes,such as metabolism,cell adhesion,growth and proliferation,cell cycle,inflammation and ***,six genes expressed by PICM-19 cells(CDO1,C22orf39,DKK2,ENPEP,GPX6,SRPX2)were completely silenced after MYC-induction in PICM-19-CSCs,suggesting that the absence of these genes may be critical for ***:MYC-driven genes may serve as promising candidates for the development of hepatocellular carcinoma therapeutics that would not have deleterious effects on other cell types in the liver.

关键词： Hepatocellular carcinoma MYC Stem cells Gene expre

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Effect of adrenalectomy on rat epididymidis

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Asian Journal of Andrology 2002年第4期4卷 273-279页

作者： Neena Nair R.S. Bedwal R.S. Mathur cell biology Laboratory Department of Zoology University of Rajasthan Jaipur-302 004 IndiaCell Biology Laboratory Department of Zoology University of Rajasthan Jaipur-302 004 IndiaCell Biology Laboratory Department of Zoology University of Rajasthan Jaipur-302 004 India

AIM: To investigate the effect of adrenalectomy (ADX) on the epididymidis of Sprague-Dawley rats. METHODS: The histological, biochemical (cholesterol protein, zinc, copper, alkaline and acid phosphatase aryl sulphatase, lactic dehydrogenase and leucine amino peptidase) and hormonal (FSH, LH and testosterone) changes of caput and cauda epididymis in ADX rats were observed. RESULTS: Organ wet weight, histological studies and morphometric measurements indicated a cellular degeneration in caput and cauda epididymis of ADX rats. Serum testosterone level was significantly lower in ADX than in sham-operated rats, while the serum FSH and LH were below the detection limit of 1 mIU/mL. The enzymatic activity was higher in ADX than in sham-operated rats. Epididymal zinc level increased whereas copper level decreased in ADX rats compared to the sham-operated. CONCLUSION: Adrenalectomy leads to degeneration of caput and cauda epididymidis epithelial cells as a result of decreased supply of testosterone.

关键词： Adrenalectomy Acid Phosphatase Alkaline Phosphatase Animals Arylsulfatases Cholesterol Comparative Study Copper Epididymis Follicle Stimulating Hormone L-Lactate Dehydrogenase Leucyl Aminopeptidase Luteinizing Hormone Male Organ Size Rats Rats, Sprague-Dawley Reference Values Research Support, Non-U.S. Gov't Testosterone Time Factors Zinc

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Cleavage of Supercoiled Circular Double-stranded DNA Induced by a Eukaryotic Cambialistic Superoxide Dismutase from Cinnamomum camphora

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 609-617页

作者： Bao-Zhong WANG, Xu-Bin WEI&, and Wang-Yi LIU* State Key Laboratory of Molecular biology, Institute of Biochemistry and cell biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Shanghai 200031, China State Key Laboratory of Molecular biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

A eukaryotic cambialistic superoxide dismutase (SOD) has been purified to homogeneity from mature seeds of the disease- and insect-resistant camphor tree (Cinnamomum camphora). Besides the known role of this SOD in protecting cells against oxidative stress, it can induce the cleavage of supercoiled double-stranded DNA into nicked and linear DNA. It can not cleave linear DNA or RNA, demonstrating there is no DNase or RNase in the purified cambialistic SOD. Furthermore, the SOD can linearize circular pGEM-4Z DNA that is relaxed by topoisomerase I. This result indicates that the DNA-cleaving activity re- quires substrates being topologically constrained. The supercoiled DNA-cleaving activity of the cambialistic SOD can be inhibited by either SOD inhibitor (azide) or catalase and hydroxyl radical scavengers (ethanol and mannitol). The chelator of iron, diethylenetriaminepentaacetic acid (DTPA), also inhibits the supercoiled DNA-cleaving activity. These results show that the dismutation activity is crucial for the supercoiled DNA cleavage. The modification of tryptophan residue of the cambialistic SOD with N-bromosuccinimide (NBS) shows that these two activities are structurally correlative. The reaction mechanism is proposed that the hydroxyl radical formed in a transition-metal-catalyzing Fenton-type reaction contributes to the DNA- cleaving activity. In addition, the cleavage sites in supercoiled pGEM-4Z DNA are random.

关键词： cambialistic SOD Fenton-type reaction hydroxyl radical relaxed circular duplex DNA supercoiled DNA cleavage

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Retinal ganglion cell neuroprotection by growth factors and exosomes:lessons from mesenchymal stem cells

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Neural Regeneration Research 2018年第2期13卷 228-229页

作者： Ben Mead Stanislav Tomarev Section of retinal ganglion cell biology laboratory of retinal cell and molecular biologynational eye institutenational institutes of healthBethesdaMDUSA

Retinal ganglions cells （RGCs） are responsible for propagating electrochemical information from the eye to the brain along their axons which make up the optic nerve. The loss of RGCs is characteristic in several conditions such as glaucoma and trau- matic optic neuropathy and leads to visual loss and blindness. While no therapy exists to directly treat RGCs, the use of bone marrow-derived mesenchymal stem cells （BMSCs） has shown promise in eliciting significant RGC neuroprotection.

关键词： BMSCs Retinal ganglion cell neuroprotection by growth factors and exosomes lessons from mesenchymal stem cells PDGFR

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PIWI proteins and their interactors in piRNA biogenesis,germline development and gene expression

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National Science Review 2014年第2期1卷 205-218页

作者： Hsueh-Yen Ku Haifan Lin Yale Stem cell Center and Department of cell biology Yale University School of Medicine

PIWI-interacting RNAs(piRNAs) are a complex class of small non-coding RNAs that are mostly 24–32nucleotides in length and composed of at least hundreds of thousands of species that speciically interact with the PIWI protein subfamily of the ARGONAUTE family. Recent studies revealed that PIWI proteins interact with a number of proteins, especially the TUDOR-domain-containing proteins, to regulate piRNA biogenesis and regulatory function. Current research also provides evidence that PIWI proteins and pi RNAs are not only crucial for transposon silencing in the germline, but also mediate novel mechanisms of epigenetic programming, DNA rearrangements, m RNA turnover, and translational control both in the germline and in the soma. hese new discoveries begin to reveal an exciting new dimension of gene regulation in the cell.

关键词： PIWI piRNA TDRD epigenetic RNA decay translational regulation

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Differential activation of intra-S-phase checkpoint in response to tripchlorolide and its effects on DNA replication

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cell Research 2004年第3期14卷 227-233页

作者： Yan REN, Jia Rui WU Laboratory of Molecular cell biology, Institute of Biochemistry and cell biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China. Laboratory of Molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China

DNA replication is tightly regulated during the S phase of the cell cycle, and the activation of the intra-S-phase checkpoint due to DNA damage usually results in arrest of DNA synthesis. However, the molecular details about the correlation between the checkpoint and regulation of DNA replication are still unclear. To investigate the connections between DNA replication and DNA damage checkpoint, a DNA-damage reagent, tripchlorolide, was applied to CHO (Chinese ovary hamster) cells at early- or middle-stages of the S phase. The early-S-phase treatment with TC significantly delayed the progression of the S phase and caused the phosphorylation of the Chkl checkpoint protein, whereas the middle-S-phase treatment only slightly slowed down the progression of the S phase. Furthermore, the analysis of DNA replication patterns revealed that replication pattern II was greatly prolonged in the cells treated with the drug during the early-S phase, whereas the late-replication patterns of these cells were hardly dete

关键词： intra-S-phase checkpoint DNA replication DNA damage tripchlorolide.

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Induction of mitochondrion-mediated apoptosis of CHO cells by tripchloro lide

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cell Research 2003年第4期13卷 295-300页

作者： YAN REN, LEI XIONG, JIA RUI WULaboratory of Molecular cell biology, Institute of Biochemistry and cell biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China 1Laboratory of Molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China 1Laboratory of Molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China 1Laboratory of Molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China

Tripchlorolide (TC) is a potent antitumor reagent purified from a Chinese herb Tripterygium Wilfordii Hook. f.. However, its cellular effects and mechanism of action are unknown. We showed here that TC induced apoptosis of Chinese Hamster Ovary (CHO) cells in time- and dose-dependent manners. TC resulted in the degradation of Bcl-2, the translocation of Bax from the cytosol to mitochondria, and the release of cytochrome c from mitochondria. Stable overexpression of human Bcl-2 could reduce the apoptosis of TC treated cells by blocking the translocation of Bax and the release of cytochrome c. These results indicate that TC induces apoptosis of CHO cell by activating the mitochondrion-mediated apoptotic pathway involving the proteins of Bcl-2 family and cytochrome c.

关键词： apoptosis mitochondrial pathway CHO cell.

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Sinoatrial node pacemaker cells: cardiomyocyte- or neuron-like cells?

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Protein & cell 2021年第7期12卷 518-519页

作者： Bin Zhou State Key Laboratory of cell biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China

The sinoatrial node(SAN)is the headquarter of heartbeat throughout our lifetime(Lakatta et al.,2010;Cingolani et al.t 2018;Peters et al.,2020).Every beat of the heart is triggered by a bioelectric pulse spontaneously released by SAN pacemaker cells(SANPCs)(Yaniv et al.,2014;Yavari et al.,2017).In adult human heart,the SAN is a crescent-shaped structure of 1-2 cm long and 0.5 cm wide,which is located at the junction of the superior vena cava and the right atrium and lies along the sulcus terminalis(John et al.,2016).However,the nature of SANPCs remains incompletely *** general,SANPCs have long been considered as specialized cardiomyocytes(Van Eif et al.,2018;Linscheid et al.,2019;Galang et al.,2020;).

关键词： incomplete specialized lifetime

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Growth induction of hepatic stimulator substance in hepatocytes through its regulation on EGF receptors

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cell Research 1999年第1期9卷 37-49页

作者： AN WEI XIAO JUN LIU TIAN GUANG LEI JIE DAI GUO GUANG DU(Department of cell biology, Capital University of Medical Sciences, Beijing 100054, China) 1Department of cell biology Capital University of Medical Sciences Beijing 100054 China 1Department of cell biology Capital University of Medical Sciences Beijing 100054 China 1Department of cell biology Capital University of Medical Sciences Beijing 100054 China 1Department of cell biology Capital University of Medical Sciences Beijing 100054 China 1Department of cell biology Capital University of Medical Sciences Beijing 100054 China

The cytosolic liver-specific growth factor-hepatic stimulator substance (HSS) has been shown to be able to amplify the rat hepatocyte proliferation responded to EGF. In order to get more insight into the mechanism, the regulatory effect of HSS on EGF-receptor(EGF-R) and the receptor phosphorylation at molecular level was studied. HSS partially purified from weanling rat liver was given to cultured hepatocytes and its influence on EGF-R specific binding and internalization as well as mRNA expression were investigated. The results showed that preincubation of hepatocytes with HSS could lead to an increase in [125I]-EGF binding to its receptors and inhibit EGFinduced receptor down-regulation. Furthermore, the overexpression of EGF-R mRNA stimulated by HSS was seen during 2-12 h after the incubation. Additionally, it was demonstrated with human hepatoma sMMC-7721 cells in Western blot that the EGF-R expression and the receptor autophosphorylation were increased with dose/timedependency after HSS treatment. These results strongly suggest that the mechanism of HSS action on hepatocyte growth might be related to its modulation on EGF-R and receptor-mediated signaling transduction.

关键词： EGF receptor hepatic stimulator substance hepatocyte proliferation

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Generation and application of human iPS cells

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Chinese Science Bulletin 2009年第1期54卷 9-13页

作者： CUI Chun RAO LingJun CHENG LinZhao XIAO Lei Laboratory of Molecular cell biology Key Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology the Cell Bank/Stem Cell bank Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Stem cell Program Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA

Human embryonic stem (ES) cells are capable of unlimited proliferation and maintenance of pluripo-tency in vitro;these properties may lead to potential applications in regenerative medicine. However,immune rejection hampers the allogenic application of human ES cells. Over-expression of several specific transcription factors has been used to reprogram human adult cells into induced pluripotent stem (iPS) cells,which are similar to hESCs in many aspects. The iPS technique makes it possible to produce patient-specific pluripotent stem cells for transplantation therapy without immune rejection. However,some challenges remain,including viral vector integration into the genome,the existence of exogenous oncogenic factors,and low induction efficiency. Here,we review recent advances in human iPS methodology,as well as remaining challenges and its potential applications.

关键词：胚胎干细胞多能干细胞分化移植

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