Growth induction of hepatic stimulator substance in hepatocytes through its regulation on EGF receptors

作     者：AN WEI XIAO JUN LIU TIAN GUANG LEI JIE DAI GUO GUANG DU(Department of Cell Biology, Capital University of Medical Sciences, Beijing 100054, China) 

作者机构：1Department of Cell Biology Capital University of Medical Sciences Beijing 100054 China 1Department of Cell Biology Capital University of Medical Sciences Beijing 100054 China 1Department of Cell Biology Capital University of Medical Sciences Beijing 100054 China 1Department of Cell Biology Capital University of Medical Sciences Beijing 100054 China 1Department of Cell Biology Capital University of Medical Sciences Beijing 100054 China 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：1999年第9卷第1期

页      面：37-49页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：国家自然科学基金 

主　　题：EGF receptor hepatic stimulator substance hepatocyte proliferation 

摘      要：The cytosolic liver-specific growth factor-hepatic stimulator substance (HSS) has been shown to be able to amplify the rat hepatocyte proliferation responded to EGF. In order to get more insight into the mechanism, the regulatory effect of HSS on EGF-receptor(EGF-R) and the receptor phosphorylation at molecular level was studied. HSS partially purified from weanling rat liver was given to cultured hepatocytes and its influence on EGF-R specific binding and internalization as well as mRNA expression were investigated. The results showed that preincubation of hepatocytes with HSS could lead to an increase in [125I]-EGF binding to its receptors and inhibit EGFinduced receptor down-regulation. Furthermore, the overexpression of EGF-R mRNA stimulated by HSS was seen during 2-12 h after the incubation. Additionally, it was demonstrated with human hepatoma sMMC-7721 cells in Western blot that the EGF-R expression and the receptor autophosphorylation were increased with dose/timedependency after HSS treatment. These results strongly suggest that the mechanism of HSS action on hepatocyte growth might be related to its modulation on EGF-R and receptor-mediated signaling transduction.