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Deconstructing brain-derived neurotrophic factor actions in adult brain circuits to bridge an existing informational gap in neuro-cell biology

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Neural Regeneration Research 2016年第3期11卷 363-367页

作者： Heather Bowling Aditi Bhattacharya Eric Klann Moses V.Chao Center for Neural Science New York University Center for Brain Development and Repair Institute for Stem Cell Biology and Regenerative Medicine departments of cell biology Physiologyand Neuroscience and PsychiatryKimmel Center for Biology and Medicine at the Skirball InstituteNew York University School of Medicine

Brain-derived neurotrophic factor（BDNF） plays an important role in neurodevelopment,synaptic plasticity,learning and memory,and in preventing *** decades of investigations into downstream signaling cascades and changes in cellular processes,the mechanisms of how BDNF reshapes circuits in vivo remain *** informational gap partly arises from the fact that the bulk of studies into the molecular actions of BDNF have been performed in dissociated neuronal cultures,while the majority of studies on synaptic plasticity,learning and memory were performed in acute brain slices or in vivo.A recent study by Bowling-Bhattacharya et al.,measured the proteomic changes in acute adult hippocampal slices following treatment and reported changes in proteins of neuronal and non-neuronal origin that may in concert modulate synaptic release and secretion in the *** this paper,we place these findings into the context of existing literature and discuss how they impact our understanding of how BDNF can reshape the brain.

关键词： BONLAC BDNF adult slice proteomics neuroproteomics SILAC BONCAT hippocampus proteinsynthesis

Pleiotropic attenuating effect of Ginkgo biloba against isoprenalineinduced myocardial infarction via improving Bcl-2/mTOR/ERK1/2/Na^(+) , K^(+) -ATPase activities

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Chinese Herbal Medicines 2024年第2期16卷 282-292页

作者： Jerome Ndudi Asiwe Abodunrin Adebayo Ojetola Nwoke Enekabokom Ekene Esthinsheen Osirim Anthony Chibuzor Nnamudi Benjamin Oritsemuelebi Jackson Erozueme Onuelu Nicholas Asiwe Harrison Ogheneochuko Eruotor Saviour Inegbenehi Department of physiology Delta State UniversityAbraka 1Nigeria Department of physiology University of IbadanIbadan 3017Nigeria Department of physiology Adeleke UniversityEde 250Nigeria Department of Pharmacology Rivers State UniversityNkpolu-Oroworukwo 5080Nigeria Department of physiology Redeemer’s UniversityEde 230Nigeria Department of Biochemistry PAMO University of Medical SciencesPort Harcourt 500211Nigeria Department of Pharmacology Delta State UniversityAbraka 1Nigeria Department of Anatomy University of Port HarcourtChoba 5323Nigeria Department of Medical Laboratory Sciences University of Port HarcourtChoba 5323Nigeria

Objective:Myocardial infarction(MI)is linked to an imbalance in the supply and demand of blood oxygen in the heart ***-blockers and calcium antagonists are just two of the common medications used to treat ***,these have reportedly been shown to be either ineffective or to have undesirable side *** of Ginkgo biloba leaves(GBE),a Chinese herbal product offers special compatibility benefits in therapeutic settings relating to inflammatory diseases and oxidative *** order to better understand how GBE affects MI in rats insulted by isoprenaline(ISO),the current study was ***:The heart weight index,serum lipid profile,cardiac marker enzymes,endogenous antioxidants[catalase(CAT),superoxide dismutase(SOD),glutathione(GSH),nitrites and malondialdehyde(MDA)],inflammatory mediators[tumour necrosis factor alpha(TNF-a)and interleukin-6(IL-6)],immunohistochemical expressions of B-cell lymphoma factor-2(Bcl-2),extracellular signal-regulated kinase(ERK1/2),and mammalian target of rapamycin(mTOR)and histopathological analysis were used to assess the cardioprotective properties of ***:The findings showed that GBE effectively attenuated myocardial infarction by boosting the body’s natural antioxidant defense system and reducing the release of inflammatory cytokines as well as heart injury marker *** expression of Bcl-2,ERK1/2 and mTOR was increased while the histomorphological alterations were ***:The cardioprotective effects of GBE may be due to a mechanism involving increased Bcl-2/mTOR/ERK1/2/Na^(+),K^(+)-ATPase activity.

关键词： antioxidants extracellular signal-regulated kinase Ginkgo biloba extract isoprenaline mammalian target of rapamycin myocardial infarction Na^(+),K^(+)-ATPase

Mechanical regulation of lipid and sugar absorption by Piezo1 in enterocytes

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3576-3590页

作者： Tian Tao Qing Shu Yawen Zhao Wenying Guo Jinting Wang Yuhao Shi Shiqi Jia Hening Zhai Hui Chen Cunchuan Wang Geyang Xu Department of physiology School of MedicineJinan UniversityGuangzhou 510632China Department of Metabolic and Bariatric Surgery the First Affiliated Hospital of Jinan UniversityGuangzhou 510630China Endoscopy Center the First Affiliated Hospital of Jinan UniversityGuangzhou 510630China Biotherapy Center Cell-gene Therapy Translational Medicine Research Centerthe Third Affiliated HospitalSun Yat-sen UniversityGuangzhou 510630China Key Laboratory of Viral Pathogenesis&Infection Prevention and Control(Jinan University) Ministry of EducationGuangzhou 510632China

Obesity is primarily caused by excessive intake as well as absorption of sugar and *** surge in distention pressure and intestinal motility accelerates the absorption of *** response of intestinal epithelial cells to mechanical stimulation is not fully ***1,a mechanosensitive ion channel,is widely expressed throughout the digestive ***,its function in intestinal nutrient absorption is not yet *** our study,excessive lipid deposition was observed in the duodenum of obese patients,while duodenal Piezo1-CaMKK2-AMPKa was decreased when compared to normal-weight *** high-fat diet condition,the Piezo1iKO mice exhibited abnormally elevated sugar and lipid absorption as well as severe lipid deposition in the duodenum and *** phenotypes were mainly caused by the inhibition of duodenal CaMKK2-AMPKa and the upregulation of SGLT1 and *** contrast,Yoda1,a Piezo1 agonist,was found to reduce intestinal lipid absorption in diet induced obese *** of Piezo1,stretch and Yoda1 inhibited lipid accumulation and the expression of DGAT2 and SGLT1,whereas knockdown of Piezo1 stimulated lipid accumulation and DGAT2 in Caco-2 *** study reveals a previously unexplored mechanical regulation of nutrient absorption in intestinal epithelial cells,which may shed new light on the therapy of obesity.

关键词： Piezo1 DGAT2 SGLT1 Lipid metabolism Obesity Mechanical sensing Intestinal lipid absorption Intestinal glucose absorption

Vanillylacetone attenuates cadmium chloride-induced hippocampal damage and memory loss through upregulation of nuclear factor erythroid 2-related factor 2 gene and protein expression

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Neural Regeneration Research 2024年第12期19卷 2750-2759页

作者： Fahaid H.A.L-Hashem Salah O.Bashir Amal F.Dawood Moutasem S.Aboonq Ismaeel Bin-Jaliah Abdulaiziz M.Al-Garni Mohamed D.Morsy Department of physiology College of MedicineKing Khalid UniversityAbhaSaudi Arabia Department of Basic Medical Sciences College of MedicinePrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh 11671Saudi Arabia Department of physiology College of MedicineTaibah UniversityAl-Madinah Al-MunawwarahSaudi Arabia Department of Medicine College of MedicineKing Khalid UniversityAbhaSaudi Arabia

Memory loss and dementia are major public health concerns with a substantial economic *** stress has been shown to play a crucial role in the pathophysiology of hippocampal damage-induced memory *** investigate whether the antioxidant and anti-inflammatory compound vanillyla cetone(zingerone) can protect against hippocampal damage and memory loss induced by cadmium chloride(CdCl_(2)) administration in rats,we explo red the potential involvement of the nuclear factor erythroid 2-related factor 2(Nrf2) signaling pathway,which is known to modulate oxidative stress and *** healt hy male Wistar rats were divided into five groups:vehicle-treated(control),vanillylacetone,CdCl_(2),vanillylacetone+ CdCl_(2),vanillylacetone+ CdCl_(2)+ brusatol(a selective pharmacological N rf2inhibitor) *** effectively attenuated CdCl_(2)-induced damage in the dental gyrus of the hippocampus and improved the memory function assessed by the Morris Water Maze ***,vanillylacetone markedly decreased the hippocampal tissue levels of inflammatory biomarkers(interleukin-6,tumor necrosis factor-α,intracellular cell adhesive molecules) and apoptosis biomarkers(Bax and cleaved caspase-3).The control and CdCl_(2)-treated groups treated with va nillylacetone showed reduced generation of reactive oxygen species,decreased malondialdehyde levels,and increased superoxide dismutase and glutathione activities,along with significant elevation of nuclear Nrf2 mRNA and protein expression in hippocampal *** the protective effects of vanillylacetone we re substantially blocked by the co-administration of brusatol(a selective N rf2 inhibitor).Va nillylacetone mitigated hippocampal damage and memory loss induced by CdCl_(2),at least in part, by activating the nuclear transcription factor ***,vanillylacetone exerted its potent antioxidant and antiinflammatory actions.

关键词： hippocampus neuroprotective Nrf2 gene oxidative stress vanillylacetone

Life after the birth of the mitochondrial Na^+/Ca^(2+) exchanger,NCLX

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Science China(Life Sciences) 2015年第1期58卷 59-65页

作者： NITA Lulia I. HERSHFINKEL Michal SEKLER Israel Department of physiology and cell biology Faculty of Health SciencesBen-Gurion University of the Negev

Powered by the mitochondrial membrane potential,Ca2+ permeates the mitochondria via a Ca2+ channel termed Ca2+ uniporter and is pumped out by a Na+/Ca2+ exchanger,both of which are located on the inner mitochondrial *** Ca2+ transients are critical for metabolic activity and regulating global Ca2+ *** the other hand,failure to control mitochondrial Ca2+ is a hallmark of ischemic and neurodegenerative *** their importance,identifying the uniporter and exchanger remains elusive and their inhibitors are *** review will focus on the mitochondrial exchanger,initially describing how it was molecularly identified and linked to a novel member of the Na+/Ca2+ exchanger superfamily termed *** control of NCLX expression provides a selective tool to determine its physiological role in a variety of cell *** lymphocytes,NCLX is essential for refilling the endoplasmic reticulum Ca2+ stores required for antigen-dependent *** of NCLX with the store-operated channel in astroglia controls Ca2+ influx and thereby neuro-transmitter release and cell *** refilling of the Ca2+ stores in the sarcoplasmic reticulum,which is controlled by NCLX,determines the frequency of action potential and Ca2+ transients in *** is emerging as a hub for integrating glucose-dependent Na+ and Ca2+ signaling in pancreatic β cells,and the specific molecular control of NCLX expression resolved the controversy regarding its role in neurons and β *** studies on an NCLX knockdown mouse model and identification of human NCLX mutations are expected to determine the role of mitochondrial Ca2+ efflux in organ activity and whether NCLX inactivation is linked to ischemic and/or neurodegenerative ***-function analysis and protein analysis will identify the NCLX mode of regulation and its partners in the inner membrane of the mitochondria.

关键词： NCLX MCU mitochondrial Ca^2+ signaling Na^+/Ca^2+ exchanger Na+ signaling

SPONTANEOUS POTENTIALS OF EMBRYONIC EPITHELIUM IN URODELES (CYNOPS ORIENTALIS)

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Science China Chemistry 1982年第11期25卷 1179-1185页

作者：戴荣禧孙以安 Shanghai Institute of physiology Academia Sinica Shanghai Institute of cell biology

The spontaneous potentials (SPs) of nerveless embryonic epithelium of Cynops orientaliswere observed by conventional intracellular micro-electrode technique. The amplitude and fre-quency of SPs of epithelial cells and the course of initiation and decline were recorded. TheSPs can occur repeatedly at the stages when the embryonic epithelium is able to conduct exci-tation. The SP is very similar to the evoked propagatable potential, but has shorter *** amplitudes and frenquencies of SPs differ in the different embryos and in differentepithelial cells in the same embryo. The excitable epithelial cells of the embryo may lose theirconductivity when the SPs come to a stable phase both in amplitude and frequency. And theconductivity can recover again after the subsiding of SPs. The SPs can be eliminated by tetro-dotoxin (TTX), but they are not affected by the treatment of cobalt chloride.

关键词： SPONTANEOUS POTENTIALS OF EMBRYONIC EPITHELIUM IN URODELES CYNOPS ORIENTALIS

Adrenomedullin and endocrine control of immune cells during pregnancy

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cellular & Molecular Immunology 2014年第5期11卷 456-459页

作者： Brooke C Matson Kathleen M Caron departments of cell biology & physiology University of North Carolina at Chapel Hill Chapel Hill NC USA Genetics University of North Carolina at Chapel Hill Chapel Hill NC USA

The immunology of pregnancy is complex and incompletely understood. Aberrant immune activity in the decidua and in the placenta is believed to play a role in diseases of pregnancy, such as infertility, miscarriage, fetal growth restriction and preeclampsia. Here, we briefly review the endocrine control of uterine natural killer cell populations and their functions by the peptide hormone adrenomedullin. Studies in genetic animal models have revealed the critical importance of adrenomedullin dosage at the maternal-fetal interface, with cells from both the maternal and fetal compartments contributing to essential aspects underlying appropriate uterine receptivity, implantation and vascular remodeling of spiral arteries. These basic insights into the crosstalk between the endocrine and immune systems within the maternal- fetal interface may ultimately translate to a better understanding of the functions and consequences of dysregulated adrenomedullin levels in clinically complicated pregnancies.

关键词： adrenomedullin preeclampsia pregnancy spiral artery remodeling uNK cells

The role of muscle LIM protein in the nervous system

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Neural Regeneration Research 2019年第11期14卷 1907-1908页

作者： Daniel Terheyden-Keighley Dietmar Fischer Department of cell physiology Faculty of Biology and Biotechnology Ruhr University Bochum

Unlike the peripheral nervous system (PNS), the central nervous system (CNS) has a low intrinsic regenerative capacity and has mechanisms that actively suppress axon regrowth, for example, glial scarring and myelin inhibition (Fischer, 2012). Even in the PNS, which has the principle ability to regenerate injured axons, functional recovery remains limited, particularly in cases where the nerve target has become unreceptive to re-innervation over time due to an insufficient axonal growth rate (Diekmann and Fischer, 2015). Progress towards robust neuroregenerative therapies depends upon an understanding of the relevant signaling and cytoskeletal proteins that drive and control axon extension. Muscle LIM protein (MLP), also known as cysteine and glycine-rich protein 3, was recently discovered to be one such protein that is expressed in regenerating rat neurons and whose overexpression can promote the axon regeneration of adult central, and peripheral neurons of different species (Levin et al., 2019).

关键词： LIM nervous system peripheral nervous system(PNS) central nervous system(CNS)

In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

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Microsystems & Nanoengineering 2024年第2期10卷 251-267页

作者： Tanja Pajić Katarina Stevanović Nataša V.Todorović Aleksandar J.Krmpot Miroslav Živić Svetlana Savić-Šević Steva M.Lević Marina Stanić Dejan Pantelić Brana Jelenković Mihailo D.Rabasović Institute of physiology and Biochemistry“Ivan Djaja” Faculty of BiologyUniversity of BelgradeStudentski trg 1611158BelgradeSerbia Institute for Biological Research“Siniša Stanković” University of BelgradeNational Institute of the Republic of SerbiaBulevar Despota Stefana 14211000BelgradeSerbia Institute of Physics Belgrade University of BelgradeNational Institute of the Republic of SerbiaPregrevica 11811080BelgradeSerbia University of Belgrade Faculty of AgricultureNemanjina Street 611080BelgradeSerbia Institute for Multidisciplinary Research University of BelgradeKneza Višeslava 111030BelgradeSerbia

Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing ***,their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell ***,we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi,which enabled patch-clamp measurements on protoplasts released from hyphae.A reproducible and highly precise(diffraction-limited,submicron resolution)method for obtaining viable released protoplasts was *** release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the *** plasma membrane of the obtained protoplasts formed tight and high-resistance(GΩ)contacts with the recording *** entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 *** to previous studies using heterologously expressed channels,this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.

关键词： patch recording laser