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Cardiac BIN1(cBIN1) is a regulator of cardiac contractile function and an emerging biomarker of heart muscle health

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Science China(Life Sciences) 2017年第3期60卷 257-263页

作者： Kang Zhou Tingting Hong Department of cardiovascular surgery 2nd XiangYa HospitalCentral South University Cedars-Sinai Heart Institute Cedars-Sinai Medical Center departments of Medicine Cedars-Sinai Medical Center and UCLA

In recent decades,a cardiomyocyte membrane scaffolding protein bridging integrator 1(BIN1) has emerged as a critical multifunctional regulator of transverse-tubule(t-tubule) function and calcium signaling in *** by a single gene with 20 exons that are alternatively spliced,more than ten BIN1 protein isoforms are expressed with tissue and disease *** recently discovered cardiac alternatively spliced isoform BIN1(cBIN1 or BIN1 +13 + 17)plays a crucial role in organizing membrane microfolds within cardiac *** cBIN1-induced microfolds form functional dyad microdomains by trafficking L-type calcium channels(LTCC) to t-tubule membrane and recruiting ryanodine receptors(RyR) to junctional sarcoplasmic reticulum *** cBIN1 is transcriptionally reduced as occurs in heart failure,cBIN1-microfolds are disrupted and fail to form LTCC and RyR *** a result,impaired dyad formation limits excitation-contraction coupling thus cardiac contractility,and accumulation of orphaned leaky RyRs outside of dyads increases ventricular *** myocardial BIN1 in heart failure is also detectable at the blood level,and plasma BIN1 level predicts heart failure progression and future arrhythmias in cardiomyopathy *** we will review the recent progress in BIN1-related cardiomyocyte biology studies and discuss the diagnostic and predictive values of cBIN1 in future clinical use.

关键词： heart failure cBIN1 t-tubules calcium transient arrhythmias

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Relationship between leukocyte count and angiographical characteristics of coronary atherosclerosis

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Acta Pharmacologica Sinica 2005年第9期26卷 1057-1062页

作者： En-zhi JIA~(2,5) Zhi-jian YANG~2 Biao YUAN~3 Xiao-ling ZANG~4 Rong-hu WANG~4 Tie-bing ZHU~2 Lian-sheng WANG~2 Bo CHEN~2 Wen-zhu MA~22 departments of cardiovascular Medicine,3 Chest surgery and4 Clinical Laboratory,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China departments of cardiovascular Medicine the First Affiliated Hospital of Nanjing Medical University Nanjing China Chest surgery the First Affiliated Hospital of Nanjing Medical University Nanjing China Clinical Laboratory the First Affiliated Hospital of Nanjing Medical University Nanjing China

Aim:To explore the relationship between differential leucocyte count and coro-nary ***:The study population consisted of 507 consecu-tive patients(376 male and 131 female)who underwent coronary angiography forsuspected or known coronary *** patients’ smoking and drink-ing habits were investigated,and anthropometric measurements,serummeasurements,and hematological measurements were conducted for every *** severity of coronary atherosclerosis was defined by using Gensini’s ***-way ANOVA,Spearman’s correlation analysis,and multivariatestepwise linear regression analysis were employed to explore the relationshipbetween differential leucocyte count and coronary ***:One-way ANOVA indicated that the diastolic blood pressure,glucose,urea,creatinine,leukocyte count,neutrophil count,monocyte count,hemoglobin,and plateletcount differed among the groups according to Gensini’s score,the tertile valuesof which were used as cutoff ***’s correlation analysis suggestedthat Gensini’s score was significantly correlated with age,diastolic blood pressure,glucose,urea,creatinine,leukocyte count,neutrophil count,monocyte count,hemoglobin,and erythrocyte count,*** stepwise linearregression analysis show that neutrophil count(β=0.247,P=0.000),age(β=0.141,P=0.001),glucose(β=0.173,P=0.000),creatinine(β=0.088,P=0.063),hemoglobin(β=-0.168,P=0.013)and sex(men were coded as 1 and women were coded as 2;β=-0.121,P=0.012)were significantly independently associated with the Gensini’***:The independent association of neutrophil count with theangiographical characteristics of coronary atherosclerosis,as estimated byGensini’s score,strongly suggests that granulocytosis may play a role in thedevelopment of coronary atherosclerosis.

关键词： leukocyte count lymphocyte count monocyte count neutrophils coronary atherosclerosis Gensini’s score

Comparison of skeletal and soft tissue pericytes identifies CXCR4+ bone forming mural cells in human tissues

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Bone Research 2020年第3期8卷 286-299页

作者： Jiajia Xu Dongqing Li Ching-Yun Hsu Ye Tian Leititia Zhang Yiyun Wang Robert J.Tower Leslie Chang Carolyn A.Meyers Yongxing Gao Kristen Broderick Carol Morris Jody E.Hooper Sridhar Nimmagadda Bruno Peault Aaron W.James departments of Pathology Johns Hopkins UniversityBaltimore 21205 MDUSA Department of Microbiology Wuhan University School of Basic Medical of ScienceWuhanHubeiP.R.China Department of Oral and Maxillofacial surgery School of StomatologyChina Medical UniversityShenyangLiaoningP.R.China departments of Orthopaedics Johns Hopkins UniversityBaltimore 21205 MDUSA departments of Plastic surgery Johns Hopkins UniversityBaltimore 21205 MDUSA departments of Autopsy Service Johns Hopkins UniversityBaltimoreMD 21205USA departments of Radiology and Radiological Science bonBaltimore 21205 MDUSA UCLA and Orthopaedic Hospital Department of Orthopaedic surgery and the Orthopaedic Hospital Research Center Los AngelesCA 90095USA Center for cardiovascular Science and MRC Center for Regenerative Medicine University of EdinburghEdinburghUK

Human osteogenic progenitors are not precisely defined,being primarily studied as heterogeneous multipotent cell populations and termed mesenchymal stem cells(MSCs).Notably,select human pericytes can develop into bone-forming ***,we sought to define the differentiation potential of CD146 f human pericytes from skeletal and soft tissue sources,with the underlying goal of defining cell surface markers that typify an osteoblastogenic ***146+CD31~CD45_pericytes were derived by fluorescence-activated cell sorting from human periosteum,adipose,or dermal *** CD146+CD31—CD45 cells retained canonical features of pericytes/*** pericytes demonstrated a striking tendency to undergo osteoblastogenesis in vitro and skeletogenesis in vivo,while soft tissue pericytes did not *** analysis revealed higher CXCR4 signaling among periosteal pericytes in comparison to their soft tissue counterparts,and CXCR4 chemical inhibition abrogated ectopic ossification by periosteal ***,enrichment of CXCR4+pericytes or stromal cells identified an osteoblastic/non-adipocytic precursor *** sum,human skeletal and soft tissue pericytes differ in their basal abilities to form *** exists in soft tissue pericytes,however,and CXCR4+pericytes represent an osteoblastogenic,non-adipocytic cell ***,enrichment for CXCR4-expressing stromal cells is a potential new tactic for skeletal tissue engineering.

关键词： CD146 CXCR4 pericyte

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Angioscopic Observation of an Endoluminal Stent Graft: CT Imaging versus Angioscopic Imaging

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World Journal of cardiovascular surgery 2016年第6期6卷 87-92页

作者： Yasuhiko Kobayashi Takahiro Yamaguchi Sei Komatsu Tomoki Ohara Junichi Yoshida Mitsuhiko Takewa Satoru Takahashi Chikao Yutani Kazuhisa Kodama Shizuo Yoshida departments of cardiovascular surgery Amagasaki Central Hospital Hyogo Japan departments of cardiovascular surgery Kawachi General Hospital Osaka Japan departments of Cardiology Amagasaki Central Hospital Hyogo Japan Ohara Internal Medicine and Cardiology Clinic Hyogo Japan departments of Pathology Amagasaki Central Hospital Hyogo Japan departments of surgery Amagasaki Central Hospital Hyogo Japan

We could observe the endoluminal stent graft (SG) following thoracic endovascular aneurysm repair (TEVAR) by a coronary artery angioscope and establish intravascular visualization of SG. The patient was a 70-year-old woman with the distal aortic arch aneurysm and the descending aortic aneurysm, and debranching TEVAR were performed. After 12 months follow up, urgent hospitalization was required for chest pain, and cardiac catheter examination with a coronary artery angioscope was performed. The endoluminal SG was observed. The observation in angioscope which is a video image is better than CT that is a still image, and observation in blood vessel or SG is possible. It may be possible to observe the endoluminal SG, allowing potential investigation of an endoleak, or the covering status of the SG with the native aortic vessel wall, or the state of intimal membrane formation in the endoluminal graft.

关键词： Intravascular Visualization Angioscopy Endoluminal Stent Graft Endovascular Aneurysm Repair

Current methods for the maturation of induced pluripotent stem cellderived cardiomyocytes

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World Journal of Stem Cells 2019年第1期11卷 33-43页

作者： Pranav Machiraju Steven C Greenway departments of Pediatrics and Cardiac Sciences Alberta Children’s Hospital Research Institute Libin Cardiovascular Institute of Alberta Cumming School of MedicineUniversity of Calgary departments of Pediatrics Cardiac Sciences Biochemistry & Molecular Biology Alberta Children’s Hospital Research Institute Libin Cardiovascular Institute of Alberta Cumming School of Medicine University of Calgary

Induced pluripotent stem cells(iPSCs) were first generated by Yamanaka and colleagues over a decade ago. Since then, iPSCs have been successfully differentiated into many distinct cell types, enabling tissue-, disease-, and patientspecific in vitro modelling. cardiovascular disease is the greatest cause of mortality worldwide but encompasses rarer disorders of conduction and myocardial function for which a cellular model of study is ideal. Although methods to differentiate iPSCs into beating cardiomyocytes(iPSC-CMs) have recently been adequately optimized and commercialized, the resulting cells remain largely immature with regards to their structure and function,demonstrating fetal gene expression, disorganized morphology, reliance on predominantly glycolytic metabolism and contractile characteristics that differ from those of adult cardiomyocytes. As such, disease modelling using iPSC-CMs may be inaccurate and of limited utility. However, this limitation is widely recognized, and numerous groups have made substantial progress in addressing this problem. This review highlights successful methods that have been developed for the maturation of human iPSC-CMs using small molecules,environmental manipulation and 3-dimensional(3 D) growth approaches.

关键词： Induced pluripotent stem cells Induced pluripotent stem cell-derived cardiomyocytes Regenerative medicine Stem cell biology Translational research

Mitral Valve Repair with Artificial Chordae for Posterior Leaflet Disease

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World Journal of cardiovascular surgery 2018年第7期8卷 117-125页

作者： Yohsuke Yanase Akihito Ohkawa Junji Nakazawa Toshiyuki Maeda Shuichi Naraoka Satomi Inoue Yukihiro Niida departments of cardiovascular surgery Hokkaido Prefectural Kitami Hospital Hokkaido Japan departments of Anesthesiology Hokkaido Prefectural Kitami Hospital Hokkaido Japan

Background: Recently, the concept of “Respect rather than resect” has been proposed in an attempt to restore mitral valve function. We investigated the results of mitral valve repair for posterior leaflet disease. Methods: Between April 2008 and July 2017, mitral valve repair was performed in 78 cases at our facility. Among them, 37 cases were analyzed. We divided patients into three groups according to the repair techniques used: artificial chordae technique, which uses the anchoring-technique and measured tube technique (Group A, 23 cases), resection and suture technique (Group R, 10 cases), and artificial chordae together with resection and suture technique (Group AR, 4 cases), and compared their postoperative outcomes. Results: Residual postoperative mitral regurgitation (MR) grade in groups A, R and AR at discharge were 0.3 ± 0.4, 0.8 ± 0.9 and 0.0 ± 0.0 (p = 0.07), respectively. Mitral valve areas (cm2) in groups A, R and AR were 3.2 ± 0.6, 2.9 ± 0.6 and 3.0 ± 0.6 (p = 0.47), respectively. Freedom from severe MR at 5 years postoperatively was seen in 91.7%, 90% and 100% (p = 0.92) in groups A, R and AR, respectively. Conclusions: There was no significant difference in postoperative outcomes, as assessed echocardiographically, between the artificial chordae technique and resection and suture technique. The results of all repair techniques were satisfactory.

关键词： Mitral Valve Regurgitation Mitral Valve Repair Artificial Chordae Heart Valve Diseases

Inhibition of STAT3 expression by siRNA suppresses growth and induces apoptosis in laryngeal cancer cells

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Acta Pharmacologica Sinica 2005年第3期26卷 377-383页

作者： Li-fang GAO~1 De-qi XU~2 Lian-ji WEN~3 Xing-yi ZHANG~4 Yue-ting SHAO~1 Xue-jian ZHAO~(1,5)1 Department of Pathophysiology,Basic School of Medicine,Jilin University,Changchun 130021,China 2 Laboratory of Enteric and Sexually Transmitted Diseases,Center for Biologics Evaluation and Researeh,Food and Drug Administration,Bethesda MD,20892,USA 3 departments of ENT4 Thoracic and cardiovascular surgery,the Second Hospital of Jilin University,Changchun 130021,China Department of Pathophysiology Basic School of Medicine Jilin University Changchun China Laboratory of Enteric and Sexually Transmitted Diseases Center for Biologics Evaluation and Research Food and Drug Administration Bethesda USA departments of ENT Changchun China Thoracic and cardiovascular surgery the Second Hospital of Jilin University Changchun China

Aim:To determine the inhibitory effect of the synthetic STAT3 siRNA on theexpression of STAT3 gene in human laryngeal cancer cell lines Hep2 and to inves-tigate the effect of STAT3 siRNA on growth and apoptosis in Hep2 ***:A pair of DNA templates coding siRNA against STAT3-mRNA was synthesized toreconstruct plasmid of pSilencer1.0-U6 ***2 cells were trans-fected with RPMI-1640 media (untreated),plasmid (empty),and STAT3 siRNA,*** blot and Western blot analysis of STAT3 and pTyr-STAT3expression in Hep2 cells and Western blot analysis of Bcl-2 expression in the Hep2cell was performed 72 h after ***,flow cytometry,and AO/EBassay were used for determination of cells proliferation and apoptosis in ***:pTyr-STAT3 was markedly expressed in untreated Hep2 cells andthe vector-treated Hep2 cells,whereas pTyr-STAT3 expression was significantlyreduced in STAT3 siRNA-transfected Hep2 cells,indicating that STAT3 siRNAinhibited the activity of *** of Hep2 cells with STAT3 siRNAsignificantly inhibited STAT3 expression at both mRNA and protein level in Hep2cells and the inhibition was characterized by time-dependent ***-ment of Hep2 cells with STAT3 siRNA resulted in dose-dependent growth inhibi-tion of Hep2,this significantly increased apoptotic cell rate,and decreased Bcl-2expression level in Hep2 ***3 siRNA had an effect on induction of eitherearly or late stage ***:This study demonstrates that STAT3siRNA effectively inhibits STAT3 gene expression in Hep2 cells leading to growthsuppression and induction of apoptosis in Hep2 *** use of siRNA tech-nique may provide a novel therapeutic approach to treat laryngeal cancer andother malignant tumors expressing constitutively activated STAT3.

关键词： STAT3 RNA interference laryngeal neoplasms apoptosis

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The Journal of Biomedical Research 2016年第1期30卷 60-66页

作者： Mingzi Song Mingming Fang Liming Yu Yong Xu departments of Pathophysiology and Nursing Jiangsu Jiankang Vocational University Key Laboratory of cardiovascular Disease Department of PathophysiologyNanjing Medical University

Expression of P-selectin in injured or activated endothelia cells serves as a permissive step towards leukocyte recruitment and perpetuation of inflammation in the pathogenesis of atherosclerosis.P-selectin can be induced by pro-inflammatory stimuli via the transcription factor NF-κB,but the epigenetic mechanisms remain incompletely *** we reported that myocardin-related transcription factor A（MRTF-A）mediates the transactivation of a slew of adhesion molecules by oxidized low-density lipoprotein（oxLDL）,likely through a crosstalk with brahma-related gene 1（BRGl）,a chromatin remodeling ***,we show that MRTF-A was both sufficient and necessary for the transactivation of P-selectin gene in endothelial cells treated with TNF-α.Depletion of MRTF-A using small interfering RNA（siRNA）abrogated the binding of BRGl on the P-selectin *** of BRG1 up-regulated the activity of P-selectin promoter activity while BRGl knockdown attenuated P-selectin ***,BRGl silencing suppressed the accumulation of acetylated histone H3 and methylated histone H3K4,and altered the binding of NF-κB on the P-selectin ***,our data demonstrate an essential role for MRTF-A and BRGl in P-selectin transactivation in endothelial cells.

关键词： myocardin-related transcription factor A(MRTF-A) brahma-related gene 1(BRG1) P-selectin endothelial cell

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CT确定的T1期肺腺癌的脏层胸膜侵犯与无病生存期无关

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国际医学放射学杂志 2019年第6期 732-733页

作者： H.Kim J.M.Goo Y.T.Kim C.M.Park 戚建晨 departments of Radiology and Thoracic and cardiovascular surgery Seoul National University College of Medicine

摘要目的验证并分析CT预测切除淋巴结阴性的肺腺癌病人脏层胸膜侵犯(p VPI)的准确性和预后价值。材料与方法本回顾性队列研究包括2009—2015年手术切除的临床

关键词：肺腺癌无病生存期 CT T1