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Identification of proteins involved in lignocellulose degradation using in gel zymogram analysis combined with mass spectroscopy-based peptide analysis of gut proteins from larval Asian Ionghorned beetles, Anoplophora glabripennis

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Insect Science 2010年第3期17卷 253-264页

作者： Scott M. Geib Ming Tien Kelli Hoover departments of biochemistry and Molecular Biology departments of Entomology The Pennsylvania State University University Park Pennsylvania USA

Enzyme activities toward lignocellulose substrates were analyzed in the gut of larval Asian longhorned beetle （Anoplophora glabripennis）. Total protein was extracted from gut contents of wild collected larvae from an invasive population in Worchester, MA, USA. From these protein extracts, lignocellulolytic activities were measured （β-1,4- endoglucanase,β-1,4-glucosidase and birch wood xylanase）.β-1,4-glucosidase activity was 0.075 μmol glucose/mg protein per min, endoglucanase activity was measured at 0.41/zmol glucose/mg protein per min and xylanase activity was 0.058μmol xylose/mg protein per min. To identify specific enzymes that may provide these activities, zymogram analysis was performed to detect enzymes active toward carboxymethyl cellulose （CMC）, 4-methylumbelliferyl-β-D-glueopyranoside and birch wood xylan. Three protein bands were found to be active toward CMC, three displayed β-1,4-glucosidase, and one displayed xylanase activity. Proteins from active bands from these zymograms were then identified by in-gel trypsin digestions followed by peptide identification by matrix-assisted laser desorption ionization - time of flight - time of flight mass spectrometry （MS）. A custom A. glabripennis transcriptome database was used for peptide identification, giving highly significant matches in all MS analyses. These matches were then searched against the National Center for Biotechnology Information database to provide annotation to the transcripts and provide possible classification. From these analyses, we were able to detect enzymes active toward cellulose and xylan, and proteins putatively involved in lignocellulose degradation in the gut of this wood-feeding insect. Future research will be focused on characterizing these enzymes through cloning and expression experiments and understanding how the lignocellulose degradation system functions in the gut of this insect.

关键词： beta-glucosidase cellulase Cerambycidae endoglucanase proteomics xylanase

Over 9% efficiency achieved for all-polymer solar cells processed by a green solvent

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Science China Chemistry 2017年第8期60卷 1109-1110页

作者： Guillermo C.Bazan departments of Chemistry ＆ biochemistry and Materials University of California Santa Barbara CA 93106 USA

Bulk-heterojunction polymer solar cells （PSCs） have at- tracted considerable attention owning to their potential for fabricating flexible, light-weight and large area solar cell panels via high-throughput roll-to-roll technologies. Compared with conventional PSCs comprising small mol- ecule acceptors, such as fullerenes, all-polymer solar cells （all-PSCs） containing blends of p-type/n-type polymers in the photoactive layer provide advantages including easily tunable absorption band, enhanced absorption coefficient,

关键词： PSCs on in is for efficiency achieved for all-polymer solar cells processed by a green solvent Over 9 by

Regrowth and neuronal protection are key for mammalian hibernation:roles for metabolic suppression

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Neural Regeneration Research 2020年第11期15卷 2027-2028页

作者： Samantha M.Logan Kenneth B.Storey Institute of biochemistry Departments of Biology and ChemistryCarleton UniversityOttawaONCanada

Thought experiment:you're starving,huddled in the fetal position in a hole in the ground,with no sense of the world around you,except that you are really,really *** fact,your internal temperature can go as low as–2.9... 详细信息

关键词： really fetal suppression

From Femtochemistry to 4D Microscopy

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Science China(Physics,Mechanics & Astronomy) 2010年第6期53卷 977-986页

作者： ZHONG Dongping departments of Physics,Chemistry,and biochemistry,Programs of Biophysics,Chemical Physics,and biochemistry,191 West Woodruff Avenue,The Ohio State University,Columbus,OH 43210,USA departments of Physics Chemistry and Biochemistry Programs of Biophysics Chemical Physics and Biochemistry The Ohio State University Columbus USA

To celebrate the 10th anniversary of the Nobel Prize for Femtochemistry,the field is overviewed with several classic examples and the new extension to Femtobiology. The revolutionary breakthrough in 4D electron microscopy is briefly introduced here and a new age to structural dynamics is rising on the horizon,an exciting time and a great opportunity for China and for the world.

关键词： femtosecond dynamics molecular coherence structural dynamics and complexity

Serum amyloid A induces WISH cell apoptosis

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Acta Pharmacologica Sinica 2007年第1期28卷 73-80页

作者： Seong-ho JO Jeanho YUN Jong-min KIM Suk-hwan BAEK Yoe-sik BAE Medical Research Center for Cancer Molecular Therapy College of MedicineDong-A University Busan 602714 Korea departments of biochemistry and Cell Biology College of MedicineDong-A UniversityBusan 602714Korea departments of biochemistry and Cell Biology College of MedicineDong-A UniversityBusan 602714Korea departments of Anatomy and Cell Biology College of MedicineDong-A UniversityBusan 602714Korea Department of biochemistry and Molecular Biology College of MedicneYeungnam UniversityDaegu 705717Korea departments of 3 biochemistry and Cell Biology College of MedicineDong-A UniversityBusan 602714Korea

Aim:Serum amyloid A(SAA)is an important mammalian acute ***,weaim to investigate the effect of SAA on apoptosis and its mechanism of action inhuman amniotic WISH ***:The expression of formyl peptide receptor(FPRL1),which is reported as a SAA receptor,was tested using RT-PCR andligand binding assay with radio-labeled FPRL1 *** effect of SAA onproliferating cell population was evaluated by thymidine incorporation *** protein phosphorylation levels and caspase-3 activity were detected by West-ern blot ***:SAA inhibits thymidine incorporation in human amnioticWISH cells.A SAA-induced decrease of proliferating cell population was accom-panied with nuclear condensation and caspase-3 activation in WISH cells,sug-gesting that SAA induces WISH cell *** FPRL1 has been reportedas a SAA receptor,we investigated the effects of several FRPL1 agonists on aproliferating cell population in WISH *** the tested FPRL1 agonists,only SAA induced a decrease of proliferating cell population in WISH *** downstream signaling of SAA,we found that SAA stimulated extracellularsignal-regulated kinase and p38 kinase,which were not inhibited by pertussistoxin(PTX),ruling out the role of PTX-sensitive *** a SAA-induced decrease of proliferating cell population was not affected by PTX,sug-gesting that SAA inhibits WISH cell apoptosis in a PTX-sensitive G-protein-independent manner.A SAA-induced decrease of a proliferating cell populationwas completely blocked by PD98059 and SB203580,suggesting that mitogen-activated protein kinase activities are essentially required for the ***:SAA is a novel inducer for WISH cell apoptosis,and the PTX-insen-sitive pathway is involved in the process.

关键词： serum amyloid A apoptosis caspase-3 ex tracellular signal regulated protein kinase p38 kinase

Is adiponectin level a predictor of nonalcoholic fatty liver disease in nondiabetic male patients?

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World Journal of Gastroenterology 2005年第37期11卷 5874-5877页

作者： Haluk Sargin Mehmet Sargin Hülya Gozu Asuman Orcun Gülcan Baloglu Murat Ozisik Mesut Seker Oya Uygur-Bayramicli departments of Endocrinology and Diabetes biochemistry Internal Medicine and Gastroenterology Kartal Education and Research Hospital Istanbul Turkey

AIM: To study the levels of adiponectin in nondiabetic patients with nonalcoholic fatty liver disease (NAFLD) in comparison with control ***: Thirty-five patients who had elevated serum aminotransferase levels with bright liver and 34 healthy volunteers without liver disease were evaluated. Age,gender and body mass index (BMI) were recorded. Fasting plasma glucose, insulin, adiponectin, proinsulin and lipid profile were measured. A standard oral glucose tolerance test (OGTT) with insulin response was performed and the index of insulin resistance was calculated according to the homeostasis model assessment (HOMA) ***: According to the OGTT results, none of the participants had diabetes. Serum adiponectin levels were statistically significantly lower in patients with NAFLD than in control group (8.14±3.4 μg/mL vs12.4±9.4 μg/mL,respectively, P＜0.01). A statistically significant correlation was found between adiponectin and BMI (r: -0.33, P＜0.01),HOMA (r: -0.26, P＜0.05), proinsulin (r: -0.32, P＜0.01),AST (r: -0.25, P＜0.05), ALT (r: -0.26, P＜0.05) or GGT (r: -0.22, P＜0.05). In multiple regression analysis models,adiponectin levels were the only predictor of NAFLD in males, whereas in female group it was the ***: Low adiponectin level might be a predictor of NAFLD especially in male nondiabetics.

关键词： Nonalooholic fatty liver disease Adiponectin Gender

Hypercholesterolemia,low density lipoprotein receptor and proprotein convertase subtilisin/kexin-type 9

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The Journal of Biomedical Research 2015年第5期29卷 356-361页

作者： Hong-mei Gu Da-wei Zhang departments of Pediatrics and biochemistry Group on the Molecular and Cell Biology of LipidsUniversity of Alberta

Atherosclerotic cardiovascular disease is the main cause of mortality and morbidity in the world. Plasma levels of low density lipoprotein cholesterol （LDL-C） are positively correlated with the risk of atherosclerosis. High plasma LDL concentrations in patients with hypercholesterolemia lead to build-up of LDL in the inner walls of the arteries, which becomes oxidized and promotes the formation of foam cells, consequently initiating atherosclerosis. Plasma LDL is mainly cleared through the LDL receptor （LDLR） pathway. Mutations in the LDLR cause familiar hyperch- olesterolemia and increase the risk of premature coronary heart disease. The expression of LDLR is regulated at the transcriptional level via the sterol regulatory element binding protein 2 （SREBP-2） and at the posttranslational levels mainly through proprotein convertase subtilisin/kexin-type 9 （PCSK9） and inducible degrader of the LDLR （IDOL）. In this review, we summarize the latest advances in the studies of PCSK9.

关键词： hypercholesterolemia low density lipoprotein receptor statin atherosclerosis proprotein convertasesubtilisin/kexin -type 9

Open and Closed： The Roles of Linker Histones in Plants and Animals

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Molecular Plant 2014年第3期7卷 481-491页

作者： Ryan S. Over Scott D. Michaels departments of Biology/Molecular and Cellular biochemistry Indiana University 915 E. Third Street Bloomington IN 47405 USA

Histones package DNA in all eukaryotes and play key roles in regulating gene expression. Approximately 150 base pairs of DNA wraps around an octamer of core histones to form the nucleosome, the basic unit of chromatin. Linker histones compact chromatin further by binding to and neutralizing the charge of the DNA between nucleosomes. It is well established that chromatin packing is regulated by a complex pattern of posttranslational modifications （PTMs） to core histones, but linker histone function is less well understood. In this review, we describe the current understand- ing of the many roles that linker histones play in cellular processes, including gene regulation, cell division, and devel- opment, while putting the linker histone in the context of other nuclear proteins. Although intriguing roles for plant linker histones are beginning to emerge, much of our current understanding comes from work in animal systems. Many unanswered questions remain and additional work is required to fully elucidate the complex processes mediated by linker histones in plants.

关键词： linker histone histone H1 chromatin gene regulation development differentiation imprinting posttrans-lational modifications DNA methylation high mobility group proteins.

Iron-handling solute carrier SLC22A17 as a blood-brain barrier target after stroke

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Neural Regeneration Research 2025年第11期20卷 3207-3208页

作者： Xueqi Ren Wenlu Li Key Laboratory of Neuropharmacology and Translational Medicine of Zhejiang Province School of Pharmaceutical SciencesZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Neuroprotection Research Laboratories Departments of Radiology and NeurologyMassachusetts General HospitalHarvard Medical SchoolBostonMAUSA

The pathophysiology of ischemic stroke is complex and multifactorial,involving various forms of cell death such as apoptosis,autophagy,and necrosis.A recent study suggests that oxidative and inflammatory stress can induce ferroptosis,a specialized form of cell death characterized by the accumulation of lipid peroxides dependent on intracellular iron overload(Li and Jia,2023).

关键词： death specialized barrier