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Studies on relationship between testicular capsule and sperm transport in rat testis

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Asian Journal of Andrology 2000年第3期2卷 191-198页

作者： Da-NianQIN MaryA.Lung departmentofphysiology ShantouUniversitySchoolofMedicineShantou515031China departmentofphysiology UniversityofHongKongHongKongChina

Aim: In SD rats, histological changes in the testis were observed after bilateral capsulotomy (of the tunica albug-inea) in order to investigate the physiological role of the testicular capsule on sperm transport. Methods: Bilaterallongitudinal capsulotomy was devised to disrupt the capsular contractile function. With this technique, only the tunicavaginalis and tunica albuginea were slit open, leaving the tunica vasculosa intact to embrace the underlying testicularparenchyma. After capsulotomy, the structural changes in the seminiferous tubules, the transitional distal seminiferoussegment, and the rete testis were observed. Results: In the capsulotomized testis, there was sperm retention at thetransitional seminiferous segment and progressive degenerative changes in seminiferous tubules. Conclusion: Theresults clearly indicated that an intact testicular capsule was required for normal sperm transport from the seminiferoustubules into the rete testis. This is the first attempt to study the physiological role of the testicular capsule in intact ***: In SD rats, histological changes in the testis were observed after bilateral capsulotomy (of the tunica albug-inea) in order to investigate the physiological role of the testicular capsule on sperm transport. Methods: Bilaterallongitudinal capsulotomy was devised to disrupt the capsular contractile function. With this technique, only the tunicavaginalis and tunica albuginea were slit open, leaving the tunica vasculosa intact to embrace the underlying testicularparenchyma. After capsulotomy, the structural changes in the seminiferous tubules, the transitional distal seminiferoussegment, and the rete testis were observed. Results: In the capsulotomized testis, there was sperm retention at thetransitional seminiferous segment and progressive degenerative changes in seminiferous tubules. Conclusion: Theresults clearly indicated that an intact testicular capsule was required for normal sperm transport from the seminif

关键词： testis testicular capsule capsulotomy sperm transport

Effect of testicular capsulotomy on fertility of rats

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Asian Journal of Andrology 2001年第1期3卷 21-25页

作者： Da-NianQIN MaryA.Lung departmentofphysiology ShantouUniversitySchoolofMedicineShantou515031China departmentofphysiology UniversityofHongKongHongKongChina

Aim: To demonstrate the effect of capsulotomy on the fertility of male rats. Methods: Testicular capsulotomywas carded out in immature (21 days) and adult (60 days) male rats. The fertility of them was assessed by cohabita-tion with proestrus females overnight and 20 days later, the females were examined for impregnation. Morphologicalchanges at the site of the capsulotomy were observed under light microscope. Results: In rats capsulotomized atDay 60, the fertility was gradually depressed and all the rats completely lost their fertility 2 months post-operation. Atthat time, a partial regeneration of the capsule at the site of capsulotomy was observed. Immature rats capsulotomized atDay 21 were found to possess normal fertility at maturity. The capsulotomy site was almost completely recovered 60days post-operation. Conclusion: In male rats, testicular capsulotomy at the age of Day 60 will damage ***, when capsulotomy is performed at Day 21, fertility is preserve.(Asian J Androl 2001 Mar; 3: 21-25)

关键词： testis testicular capsulotomy fertility

Morphometric study on Leydig cells in capsulotomized testis of rats

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Asian Journal of Andrology 2002年第1期4卷 49-53页

作者： QinDN LungMA departmentofphysiology ShantouUniversitySchoolofMedicineShantou515031Ching departmentofphysiology UniversityofHongKongKHSARChina

AIM: To further clarify the changes occurred in the testicular capsulotomized rats. METHODS: In testicular capsulotomized and sham-operated rats, the cross sectional area, the nucleus diameter and the number of Leydig cells were morphologically analyzed by the Vidas Image Processing System connected to a microscope. RESULTS: In the capsulotomized animals, the cross sectional area of Leydig cells was gradually increased from 30 days onwards. There was no obvious change in the nucleus diameter of Leydig cells. However, The Leydig cell number was significantly increased from day 30 onwards. CONCLUSION: In rats, testicular capsulotomy may induce hyperplasia/hypertrophy of Leydig cells in the testis.

关键词： Animals Cell Count Cell Nucleus Image Processing, Computer-Assisted Leydig Cells Male Rats Rats, Sprague-Dawley Testis

Effect of testicular capsulotomy on secretion of testosterone and gonadotrophins in rats

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Asian Journal of Andrology 2000年第4期2卷 257-261页

作者： Da-NianQIN MaryA.Lung departmentofphysiology ShantouUniversitySchoolofMedicineShantou515031China departmentofphysiology UniversityofHongKongHongKongChina

Aim: In order to clarify further the mechanisms underlying the effect of capsulotomy on testicular function, the lev-els of testosterone, LH and FSH were observed. Methods: Intratesticular testosterone levels and LH, FSH levelsin the peripheral blood of normal, sham-operated and capsulotomized rats were detected by RIA. Results: Aftertesticular capsulotomy, there was a progressive reduction in the testosterone level in the testicular venous blood togetherwith a progressive increase in the LH and FSH levels in the peripheral blood from approximately 30 days post-capsuloto-my. Morphological changes were observed at 5-10 days after capsulotomy, i. e., far ahead of the hormonal ***: The seminiferous tubular damage after testicular capsulotomy was not caused by the reduction in testos-terone, and on the contrary, the hormonal change might be secondary to the morphological alterations. The increase inLH level most likely resulted from a negative feedback influence from the lowered testosterone level, while the increasein FSH secretion may be a feedback signal of the damaged seminiferous tubules. (Asian J Androl 2000 Dec; 2: 257-261 )

关键词： testis testicular capsulotomy testosterone LH FSH

Relationship between atrial natriuretic peptide-immunoreactive cells and microvessels in rat gastric mucosa

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Acta Pharmacologica Sinica 2006年第2期27卷 205-211页

作者： Chun-hui LI~(2,3) Zong-wei YANG~3 Zheng-ri YIN~3 Zheng JIN~3 De-gang XING~3 Lian-hua PIAO~3 Yong-chul KIM~4 Wen-xie XU~(2,5) ~2College of Life Science and Biotechnology.Shanghai Jiaotong University,Shanghai 200240,China:~3Department of Physiology.Yanbian University College of Medicine.Yanji 133000.China ~4Department of Physiology,Chungbuk National University,College of Medicine.Cheongju.Chungbuk 361-763,Korea CollegeofLifeScienceandBiotechnology ShanghaiJiaotongUniversityShanghai200240China departmentofphysiology YanbianUniversityCollegeofMedicineYanji133000China departmentofphysiology ChungbukNationalUniversity

Aim:To investigate the ultrastructural localization of atrial natriuretic peptide(ANP)-synthesizing cells and the relationship between ANP-synthesizing cellsand microvessels in rat gastric ***:Immunohistochemistry tech-niques and postembedding immunoelectron microscopy techniques were used tovalidate the findings regarding the expression of ANP-synthesizing cells and theultrastructural localization of ANP-synthesizing cells in the gastric ***-tochemistry techniques and the tannic acid-ferric chloride method(TA-Fe stain-ing method)were used to reveal microvessel density and the distribution of ANP-synthesizing cells in different regions of the ***:Cells expressingANP were localized and ANP-synthesizing cells were identified as enterochroma-ffin(EC)cells in the gastric ***-synthesizing cells existed in differentregions of the *** percentage ANP-synthesizing cells in the mucosawas greatest in the fundus(46.7%e5.3%),intermediate in the antrum(40.1%±4.5%),and least in the body(21.6%±3.6%).There was a positive relationshipbetween the percentage of ANP-synthesizing cells and the density of microvesselsin the antral mucosa,but not in the fundus or body ***:ANP issynthesized by EC cells in rat gastric mucosa,and ANP-synthesizing cells aremost dense in the gastric *** may act not only as a regional autocrineand/or paracrine regulator,but also as an endocrine regulatory peptide in thegastrointestinal tract.

关键词： atrial natriuretic factor enterochromaffin cells blood vessels gastric mucosa

Store-operated calcium channels and pro-inflammatory signals

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Acta Pharmacologica Sinica 2006年第7期27卷 813-820页

作者： Wei-chiao CHANG~1 Department of Physiology,Anatomy and Genetics,University of Oxford,Oxford,UK departmentofphysiology AnatomyandGeneticsUniversityofOxfordOxfordUK

In non-excitable cells such as T lymphocytes,hepatocytes,mast cells,endotheliaand epithelia,the major pathway for calcium(Ca2+)entry is through store-oper-ated Ca2+channels in the plasma *** channels are activated by theemptying of intracellular Ca2+stores,however,neither the gating mechanism northe downstream targets of these channels has been clear ***,Ireview some of the proposed gating mechanisms of store-operated Ca2+channelsand the functional implications in regulating pro-inflammatory signals.

关键词： calcium channels store-operated Ca2+ entry capacitative Ca2+entry calcium signaling mast cells

Effect of resveratrol on L-type calcium current in rat ventricular myocytes

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Acta Pharmacologica Sinica 2006年第2期27卷 179-183页

作者： Li-ping ZHANG Jing-xiang YIN Zheng LIU Yi ZHANG Qing-shan WANG~1 Juan ZHAO Department of Physiology,Institute of Basic Medicine,Hebei Medical University.Shijiazhuang 050017.China departmentofphysiology InstituteofBasicMedicineHebeiMedicalUniversityShijiazhuang050017China

Aim:To study the effect of resveratrol on L-type calcium current(ICa-L)in isolatedrat ventricular myocytes and the mechanisms underlying these ***:ICa-Lwas examined in isolated single rat ventricular myocytes by using the wholecell patch-clamp recording ***:Resveratrol(10-40 μmol/L)re-duced the peak amplitude of ICa-Land shifted the current-voltage(I-V)curveupwards in a concentration-dependent ***(10,20,40 μmol/L)decreased the peak amplitude of ICa-Lfrom -14.2±1.5 pA/pF to -10.5±1.5 pA/pF(P<0.05),-7.5±2.4 pA/pF(P<0.01),and -5.2±1.2 pA/pF(P<0.01),***(40 μmol/L)shifted the steady-state activation curve of ICa-Lto theright and changed the half-activation potential(V0.5from -19.4+0.4 mV to-15.4±1.9 mV(P<0.05).Resveratrol at a concentration of 40 μmol/L did notaffect the steady-state inactivation curve of ICa-L,but did markedly shift the time-dependent recovery curve of ICa-Lto the right,and slow down the recovery ofICa-Lfrom *** orthovanadate(Na3VO4;1 mmol/L),a potentinhibitor of tyrosine phosphatase,significantly inhibited the effects of resveratrol(P<0.01).Conclusion:Resveratrol inhibited ICa-Lmainly by inhibiting the activa-tion of L-type calcium channels and slowing down the recovery of L-type cal-cium channels from *** inhibitory effect of resveratrol was medi-ated by the inhibition of protein tyrosine kinase in rat ventricular myocytes.

关键词： resveratrol patch-clamp techniques myocardium L-type calcium channels

Leptin: a multifunctional hormone

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Cell Research 2000年第2期10卷 81-92页

作者： HUANG Lu, CAI LI(Tel: (214)-648-3340 Fax: (214)-648-9191 E-mail: li03@utsw.swmed.edu)(1 Touchstone Center for Diabetes Research1 Departments of Physiology and,2 Departments of Internal MedicineThe University of Texas Southwestern Medical Center5323 H. departmentofphysiology TheUniversityofTexasSouthwesternMedicalCenterDallas75390-8854USA.

Leptin is the protein product encoded by the obese (ob)gene. It is a circulating hormone produced primarily by the adipose tissue. ob/ob mice with mutations of the gene encoding leptin become morbidly obese, infertile, hyperphagic, hypothermic,and diabetic. Since the cloning of leptin in 1994, our knowledge in body weight regulation and the role played by leptin has increased substantially. We now know that leptin signals through its receptor, OB-R, which is a member of the cytokine receptor superfamily. Leptin serves as an adiposity signal to inform the brain the adipose tissue mass in a negative feedback loop regulating food intake and energy expenditure. Leptin also plays important roles in angiogenesis, immune function, fertility and bone formation. Humans with mutations in the gene encoding leptin are also morbidly obese and respond to leptin treatment,demonstrating that enhancing or inhibiting leptin’s activities in vivo may have potential therapeutic benefits.

关键词： Leptin OB-R hypothalamus adipose tissue obesity diabetes cytokine receptor

Early association of electrocardiogram alteration with infarct size and cardiac function after myocardial infarction

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Journal of Zhejiang University Science 2004年第4期5卷 494-498页

作者：陶则伟黄元伟夏强傅军赵志宏陆贤 BRUCEI.C. DepartmentofCardiology FirstAffiliatedHospitalCollegeofMedicineZhejiangUniversityHangzhou310003China departmentofphysiology CollegeofMedicineZhejiangUniversityHangzhou310006China departmentofphysiology theUniversityofHongKongHongKongChina

Objective:Myocardial infarction (MI) is the main cause of heart failure, but the relationship between the extent of MI and cardiac function has not been clearly *** present study was undertaken to investigate early changes in the electrocardiogram associated with infarct size and cardiac function after MI. Methods: MI was induced by ligating the left anterior descending coronary artery in rats. Electrocardiograms, echocardiographs and hemodynamic parameters were assessed and myocardial infarct size was measured from mid-transverse sections stained with Masson's trichrome. Results:The sum of pathological Q wave amplitudes was strongly correlated with myocardial infarct size (r=0.920, P<0.0001), left ventricular ejection fraction (r=-0.868, P<0.0001) and left ventricular end diastolic pressure (r=0.835, P<0.0004).Furthermore, there was close relationship between MI size and cardiac function as assessed by left ventricular ejection fraction (r=-0.913, P<0.0001) and left ventricular end diastolic pressure (r=0.893, P<0.0001).Conclusion: The sum of pathological Q wave amplitudes after MI can be used to estimate the extent of MI as well as cardiac function.

关键词：心电图心脏功能心肌梗塞 MI 心力衰竭