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The　effects　of　PAOAP　on　atherosclerotic　vascular　remodeling

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中国组织化学与细胞化学杂志 2004年第3期13卷 369-370页

作者： QingChang CaixiaRen HailanTang HuameiHuang ZichengLi departmentofhistologyandembryology theFirstAffiliatedHospitalMedicalCollegeofJinanUniversityGuangdong510632P.R.ofChina DepartmentofCardiology theFirstAffiliatedHospitalMedicalCollegeofJinanUniversityGuangdong510632P.R.ofChina

Whole Cag A Gene Amplification of Helicobacter pylori and Its Fingerprinting by Restriction Fragment Length Polymorphism

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2002年第4期22卷 276-278页

作者：叶嗣颖敖杰男彭颖岳海峰廖芳胡国平徐杨张正茂 DepartmentofPathogenicOrganisms medicalcollegeofjinanuniversity CenterofCellularandMolecularBiology DepartmentofMicrobiology&InfectiousDiseases

To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to am plify the gene and Eco RΙ and Hind were used to generate the RFL P *** DNA fragm ents from 13of2 0 samples were successfully amplified and the relevant RFL P fingerprintings were *** is concluded thatthe m ethod can be used to amplify the whole Cag A gene and Cag A gene has apparent diversity of RFL P profile.

关键词： H elicobacter pylori cag A PCR RFL P

Effects of Mifepristone (RU_(486)) on Hypophysis, Ovary,Uterus and Placenta in Pregnant Rats

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Biomedical and Environmental Sciences 1998年第2期11卷 133-139页

作者： CHENHONG TONGSU－MING departmentofhistologyandembryology ShanghaiMedicalUniversityShanghai200032 DepartmentofHistologyandE

The effects of mifepristone (RU486) on the early and middle stage of pregnant rats were examined using light and electron microscopy. Two groups of pregnant rats were administered with RU486, at a dose of 1. 25 mg/rat on days 7 and 10 of pregnancy, respectively. Thereafter all the rats with middle stage had abortion with necroticdecidua at 48h post administration. However, uterus endometrium returns to normal. The number of the lipid droplets in the luteal cells increased. The level of serum progesterone was significantly lower than that of the control group. For rats with early stage pregnancy, the development of some embryos in two cases was similar to that of the control group at 48h post administration. The embryos of the remaining rats in the group were all disintegrated. Nevertheless, two rats had their uteri restored. There were no significant differences in the structure of corpus luteum, the levels of hypophyseal gonadotrophin nor the serum progesterone between the treated and the control groups. These results indicate that the anti-pregnant activity of RU486 is more effective during the middle stage pregnancy than in the early pregnancy in rats.

关键词： RU-486 PHASE

Expression and significance of Rap1A in testes ofazoospermic subjects

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Asian Journal of Andrology 2004年第1期6卷 35-40页

作者： BoYang HeWang Xiao-KangGao Bao-QiChen Yuan-QiangZhang He-LiangLiu YongWang Wei-JunQin Rong-LiangQin Guo-XingShao ChenShao DepartmentofUrology XijingHospital departmentofhistologyandembryology FourthMilitaryMedicalUniversityXi''an710033China

Aim: To evaluate the Rap1A mRNA expression and its significance in the testes of normal and azoospermic subjects. Methods: A cDNA microarray that contained Rap1A and some other genes such as RBM, EIF1 AY was used to identify the differential gene expression profiles between the normal and azoospermic testes. cDNA probes were prepared by labeling mRNA from azoospermic and normal testicular tissues through reverse transcription with Cy5-dUTP and Cy3-dUTP, respectively. The mixed cDNA probes were then hybridized with cDNA microarray (each containing 4096 unique human cDNA sequences). The fluorescent signals were scanned and the values of Cy5-dUTP and Cy3-dUTP on each spot were analyzed and calculated. In situ hybridization was employed to detect the expression of RaplA in the testes of 10 fertile and 39 azoospermic subjects. Results: One hundred and twenty-eight differentially expressed genes were found to be possibly related to azoospermia, of which 56 were up-regulated and 72, down-regulated genes. The mRNA expression of RaplA in the spermatogenic cells of azoospermic was stronger than that in those of the fertile testes. Conclusion: Rap1A may play certain roles in the development of azoospermia.

关键词： Rap1A azoospermia cDNA microarray

Anti-Tumor Effect of CDA-Ⅱ on a Human Glioma Cell

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Chinese Journal of Clinical Oncology 2005年第3期2卷 679-684页

作者： HongyanWang XueyunZhong FrankCLiu YanfangQin DepartmentofPathology MedicalCollegeofJinanUniversityGuangzhou510032China

OBJECTIVE To examine the effect of uroacitide (CDA-Ⅱ ), an extraction product from normal human urine, on proliferation and differentiation of human glioma SWO-38 cells. METHODS The effects of CDA-Ⅱ on cellular survival and colony formation were determined by MTT and colony-formation assays. The in vivo anti-tumor effect of CDA-Ⅱ was examined on transplanted SWO-38 cells in nude mice. In addition, the aterations in cell morphology induced by CDA-Ⅱ were observed by H&E staining. RESULTS Treatment of SWO-38 cells with 1-5 mg/ml of CDA-Ⅱ for 3 days, resulted in 39.49% ± 5.27%-65.05% ± 5.89% growth inhibition with an IC50 of 2.52 mg/ml. Based on the colony-formation assay, 10 days of CDA-Ⅱ treatment at a level of 0.3-2.1 mg/ml caused 23.45% ± 0.62%-96.22% ± 1.01% inhibition with an IC50 of 1.03 mg/ml. Furthermore, the inhibitory response was dose-dependent. CDA-Ⅱ at dosage of 500 mg/kg or 2,000 mg/kg per day for 4 weeks significantly suppressed the growth of human glioma SWO 38 cells in nude mice, with inhibition being 47.77% and 79.94%, respectively (P <0.05, n=10). H&E staining and light microscopy revealed that CDA-Ⅱ induced differentiation of the SWO-38 cells. CONCLUSION CDA-Ⅱ has a significant anti-tumor effect on the human glioma SWO-38 cells, and is a potential and natural anti-glioma therapeutic reagent.

关键词： CDA-Ⅱ 神经胶质瘤肿瘤细胞药物治疗治疗效果

REGULAR EXPRESSION OF DISCOIDIN DOMAIN RECEPTOR 2 IN THE IMPROVED ADJUVANT-INDUCED ANIMAL MODEL FOR RHEUMATOID ARTHRITIS

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Chinese Medical Sciences Journal 2005年第2期20卷 133-137,页

作者： WeiLi Yuan-qiangZhang Xin-pingLiu Li-boYao LanSun departmentofhistologyandembryology DepartmentofBiochemistryandMolecularBiology theFourthMilitaryUniversityXi＇an710032

Objective To investigate the expression of discoidin domain receptor 2 (DDR2) of fibroblast-like synovial cells in im- proved adjuvant-induced animal (AIA) model for rheumatoid arthritis (RA) and to provide evidence for DDR2’s antagonist use clinically. Methods AIA was modified by administrating 0.1 mL of complete Freund’s adjuvant (CFA, mixed with 5 mg Bacillus Calmette-Guerin vaccine/mL) into rats’ right hind paws and 0.125 mL tumor necrosis factor-α (2 U/mL) into right ankles and subpatellar fatty tissue. The expression of DDR2 in fibroblast-like synovial cells was assessed using immunohistochemistry, immunofluorescence histochemistry, and in situ hybridization methods. Levels of anti-collagen II antibody were measured using enzyme-linked immunosorbent assay. Results Given the terms mentioned above, we found a more practical rat model, apparently decreasing immunization time (average 3-5 days). DDR2 can be detected upon the 15th day of immunization; expression gradually increased with time going on, and reaching a peak 35 days after immunization before gradually decreasing. Serum anti-collagen II antibody showed similar expression patterns as DDR2, but reached peak later than DDR2, about 40 days after immunization. Conclusion Regular expression of DDR2 in animal models infers its important role in the pathological process of RA.

关键词： rheumatoid arthritis adjuvant-induced arthritis discoidin domain receptor 2 synovial tissue anti-collagen Ⅱ antibody

Neutral sulfate berberine modulates cytokine secretion and increases survival in endotoxemic mice

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Acta Pharmacologica Sinica 2006年第9期27卷 1199-1205页

作者： Fei LI Hua-dong WANG~2 Da-xiang LU Yan-ping WANG Ren-bin QI Yong-mei FU Chu-jie LI Department of Pathophysiology,Medical College of Jinan University,Guangzhou 510632,China DepartmentofPathophysiology MedicalCollegeofJinanUniversityGuangzhou510632China

Aim:Berberine is thought to be an immunomodulator,so the present study aimedto investigate the effect of berberine on mortality,lung and intestine injury inendotoxemic mice,and the mechanism of its ***:Mice were chal-lenged with lipopolysaccharide(LPS,28 mg/kg,ip),and neutral sulfate berbefinewas administrated *** was monitored every 12 h,and his-tology of the lungs and intestine as well as the plasma tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin-12(IL-12),IL-10,and nitric oxide(NO)levels were ***:Pretreatment with 50 mg/kg neutral sulfate ber-berine once a day for 5 days significantly decreased the mortality rate and attenu-ated tissue injury of the lungs and small intestine in mice challenged with *** stimulated a marked increase in plasma levels of TNF-α,IFN-γ,IL-12,IL-10,and *** administration of berberine significantly reduced plasma TNF-α,IFN-γ,and NO levels,but did not suppress plasma IL-12 levels in mice exposed ***,pretreatment with neutral sulfate berbefine augmented IL-10secretion stimulated by LPS in ***:Pretreatment with neutral sul-fate berberine attenuates tissue injury and improves survival in endotoxemic mice,which may be mediated,at least in part,by the inhibition of pro-inflammatorymediator production and upregulation of IL-10 *** findings mightprovide a new strategy for the treatment of endotoxemia.

关键词： lipopolysaccharide berberine tumor necrosis factor-α interleukin-10 interleukin-12 interferon-γ nitric oxide mice

Mutation of RET gene in Chinese patients with Hirschsprung's disease

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World Journal of Gastroenterology 2002年第6期8卷 1108-1111页

作者： Ji-ChengLi Shi-PingDing YingSong Min-JuLi Children’sHospital ZhejiangUniversityMedicalCollegeHangzhou310006ZhejiangProvinceChina DepartmentofLymphology DepartmentofHistologyandEmbryologyZhejiangUniversityMedicalCollegeHangzhou310031Zhe

AIM: To investigate the pathogenic mechanism of Hirschsprung's disease (HD) at the molecular level and to elucidate the relationship between RET oncogene and Chinese patients with ***: Exon 13 of RET oncogene from 20 unrelated HD patients was analyzed with polymerase chain reactionsingle strand conformation polymorphism (PCR-SSCP). The positive amplifying products were then sequenced. According to the results of SSCP and DNA sequence, SSCP was done as well for the samples from the family other members of some cases with mutated RET ***: SSCP analysis indicated that mobility abnormality existed in 4 unrelated HD patients. Direct DNA sequence analysis identified a missense mutation, T to G at the nucleotide 18 888 and a frameshift mutation at the nucleotide 18 926 insG. In a HD family, the sicked child and his father were the same heterozygous missense mutation (T to G at nucleotide 18 888).CONCLUSION: Among Chinese HD patients, RET gene mutations may exist in considerable proportion with different patterns. These new discoveries indicate that RET mutations may play an important role in the pathogenesis of unrelated HD in the Chinese population. PCR-SSCP combined with DNA sequence can be used as a tool in the genetic diagnosis of HD.

关键词： RET基因突变中国人巨结肠病分子病理

Expression and localization of Smadl, Smad2 and Smad4 proteins in rat testis during postnatal development

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Asian Journal of Andrology 2003年第1期5卷 51-55页

作者： JingHU Yuan-QiangZHANG Xin-PingLIU Rui-AnWANG YanJIN Ruo-JunXU departmentofhistologyandembryology DepartmentofBiochemistryandMolecularBiology DepartmentofOralHistologyandPathology SchoolofDentistryFourthMilitaryMedicalUniversityXi‘an710032China DepartmentofZoology UniversityofHongKongHongKongHKSARChina

Aim: To study the expression and regulation of Smadl, Smad2 and Smad4 proteins (intracellular signaling molecules of transforming growth factor-β family) in rat testis during postnatal development. Methods: The whole testes were collected from SD rats aged 3, 7, 14, 28 and 90 (adult) days. The cellular localization and developmental changes were examined by immunohistochemistry ABC method with the glucose oxidase-DAB-nickel enhancement technique. Quantitative analysis of the immunostaining was made by the image analysis system. The Smads proteins coexistence in the adult rat testis was tested by the double immune staining for CD14-Smad4 and Smad2-Smad4. The protein expression of Smad during rat testicular development was examined by means of Western blots. Results: Smadl, Smad2 and Smad4 were present throughout testicular development. The immunostaining of Smadl and Smad2 were present in spermatogenic cells. A positive immunoreactivity was located at the cytoplasm, but the nucleus was negative. Smadl was immunolocalized at the d14, d28 and adult testes, while Smad2, at the d7, d14, d28 and adult testis. There was positive irnmunoreaction in the Sertoli cells and Leydig cells as well. The immunolocalization of Smad4 was exclusively at the cytoplasm of Leydig cells and the nuclei were negative throughout the testicular development. No expression was detected in the germ cells. The results of image and statistical analysis showed that generally the expression of Smadl, Smad2 and Smad4 in the testis tended to increase gradually with the growth of the rat. Conclusion: The present data provide direct evidences for the molecular mechanism of TGF-βaction in rat testes during postnatal development and spermatogenesis.

关键词： TGF-β Smadl Smad2 Smad4 testis