Whole Cag A Gene Amplification of Helicobacter pylori and Its Fingerprinting by Restriction Fragment Length Polymorphism

作     者：叶嗣颖 敖杰男 彭颖 岳海峰 廖芳 胡国平 徐杨 张正茂 

作者机构：DepartmentofPathogenicOrganisms MedicalCollegeofJinanUniversity CenterofCellularandMolecularBiology DepartmentofMicrobiology&InfectiousDiseases 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2002年第22卷第4期

页      面：276-278页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：Thisprojectwassupported by a grant from the MinistryofPublicHealth(Serial No.:98- 1- 12 3) 

主　　题：H elicobacter pylori cag A PCR RFL P 

摘      要：To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to am plify the gene and Eco RΙ and Hind were used to generate the RFL P *** DNA fragm ents from 13of2 0 samples were successfully amplified and the relevant RFL P fingerprintings were *** is concluded thatthe m ethod can be used to amplify the whole Cag A gene and Cag A gene has apparent diversity of RFL P profile.