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The extract of Celtis choseniana Nakai alleviates testosterone-in-duced benign prostatic hyperplasia through inhibiting 5αreductase type 2 and the Akt/NF-κB/AR pathway

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Chinese Journal of Natural Medicines 2022年第7期20卷 518-526页

作者： HONG Geum-Lan KIM Tae-Won LEE Hui-Ju KIM Yae-Ji KIM Kyung-Hyun JUNG Ju-Young department of veterinary Medicine&Institute of veterinary science Chungnam National UniversityDaejeon 34134Republic of Korea

Benign prostatic hyperplasia(BPH)is a chronic male disease characterized by the enlarged prostate.Celtis choseniana Nakai(C.choseniana)is medicinally used to alleviate pain,gastric disease,and lung abscess.In this study,the effect of C.choseniana extract on BPH was investigated using testosterone-induced rats.Sprague Dawley rats were divided into five groups:control,BPH(testosterone 5 mg·kg^(−1)),Fina(finasteride 2 mg·kg^(−1)),and C.choseniana(50 and 100 mg·kg^(−1)).After four weeks of TP treatment with finasteride or C.choseniana,prostate weights and DHT levels were measured.In addition,the prostates were histopathologically examined and measured for protein kinase B(Akt)/nuclear factor-κB(NF-κB)/AR signaling,proliferation,apoptosis,and autophagy.Pro-state weight and epithelial thickness were reduced in the C.choseniana groups compared with that in the BPH group.The extract of C.choseniana acted as a 5αreductase inhibitor,reducing DHT levels in the prostate.Furthermore,the extract of C.choseniana blocked the activation of p-Akt,nuclear NF-κB activation and reduced the expression of AR and PSA compared with BPH.Moreover,the ex-pression of Bax,PARP-1,and p53 increased,while the expression of bcl-2 decreased.The present study demonstrated that C.choseni-ana extract alleviated testosterone-induced BPH by suppressing 5αreductase and Akt/NF-κB activation,reducing AR signaling and in-ducing apoptosis and autophagy in the prostate.These results suggested that C.choseniana probably contain potential herbal agents to alleviate BPH.

关键词： Apoptosis Androgen receptor Benign prostate hyperplasia Celtis choseniana Nakai 5α-Reductase type 2 NF-κB

Molecular survey on zoonotic tick—borne bacteria and chlamydiae in feral pigeons(Columba livia domestica)

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 316-319页

作者： Valentina Virginia Ebani Fabrizio Bertelloni Paolo Mani Department of Veterinary Science University of Pisa

Objective;To determine the presence of zoonotic tick-borne bacteria in feral pigeons(Columba lixia domestica) from urban areas.Methods:Spleen samples from 84 feral pigeons,found dead with traumatic injuries in urban areas,were examined by PCR to detect DNA of Anaplasma phagocytophilum,Bartonella spp.,Borrelia burgdorferi sensu lato.Coxiella burnetii.Rickettsia spp.,and Chlamydnphila spp.Results:Twenty(23.8%) pigeons were infected by tick-borne agents,in particular 2(2.38%) animals resulted positive for Bartonella spp.,5(5.95%) for Coxiella burnetii.5(5.95%) for Rickettsia spp.,13(15.47%) for Borrelia burgdorferi sensu lato.All birds scored negative for anaplasma phagocytophilum.Moreover,17(20.23%) pigeons were positive for Chlamydophila spp.and among them 10(11.9%) for Chlamydophila psittaci,Mixed infections by two or three agents were detected in 8(9.52%) animals.Conclusions:Feral pigeons living in urban and periurban areas are a hazard for the human health as source of several pathogens.The obtained results confirm pigeons as reservoirs of chlamydial agents and suggest that they may be involved in the epidemiology of zoonotic tick-borne infections too.

关键词： Pigeon(Columba livia domestica) Tick-borne bacteria Chlamydophila psittaci PCR

Molecular survey of tick-borne pathogens in Ixodid ticks collected from hunted wild animals in Tuscany, Italy

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Asian Pacific Journal of Tropical Medicine 2015年第9期8卷 702-705页

作者： Valentina Virginia Ebani Fabrizio Bertelloni Barbara Turchi Dario Filogari Domenico Cerri Department of Veterinary Science University of Pisa

Objective: To determine the prevalence of zoonotic tick-borne bacteria in feeding ticks removed from hunted wild animals. Methods: PCR was executed on DNA extracted from 77 tick pools to detect Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi sensu lato, Coxiella burnetii and Rickettsia spp. Results: A total of 432 ticks were collected: 30(6.94%) Haemaphysalis punctata, 72(16.7%) Dermacentor marginatus and 330(76.38%) Ixodes ricinus. For each animal one or two pools of 3 ticks of the same species was constituted. Seventy-seven tick pools were examined by PCR: 58(75.32%) resulted infected and among them 14(18.18%) showed co-infections. In particular, 29(37.66%) pools were positive for Bartonella spp., 23(29.87%) for Anaplasma phagocytophilum, 16(20.78%) for Rickettsia spp., and 5(6.49%) for Borrelia burgdorferi s.l. All samples were negative for Coxiella burnetii. Conclusions: The results demonstrate the presence of several zoonotic tick-borne pathogens in the studied area, and underline the risk of exposure to infections for hunters not only during the outdoor activity, but also when they manipulate hunted animals infested by infected ticks.

关键词： Ixodid ticks Tick-borne bacteria Wild animals PCR Hunters

Beta-nerve growth factor gene therapy alleviates pyridoxine-induced neuropathic damage by increasing doublecortin and tyrosine kinase A in the dorsal root ganglion

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Neural Regeneration Research 2020年第1期15卷 162-168页

作者： Hyun-Kee Cho Woosuk Kim Kwon-Young Lee Jin-Ok Ahn Jung Hoon Choi In Koo Hwang Jin-Young Chung department of veterinary Internal Medicine and Institute of veterinary science College of Veterinary MedicineKangwon National UniversityChuncheonKangwon-doSouth Korea department of Anatomy and Cell Biology College of Veterinary MedicineResearch Institute for Veterinary ScienceSeoul National UniversitySeoulSouth Korea department of Anatomy College of Veterinary MedicineKangwon National UniversityChuncheonKangwon-doSouth Korea

Beta-nerve growth factor(β-NGF)is known to be a major leading cause of neuronal plasticity.To identify the possible action mechanisms ofβ-NGF gene therapy for sciatic nerve recovery,experimental dogs were randomly divided into control,pyridoxine,and pyridoxine+β-NGF groups.We observed chronological changes of morphology in the dorsal root ganglia in response to pyridoxine toxicity based on cresyl violet staining.The number of large neurons positive for cresyl violet was dramatically decreased after pyridoxine intoxication for 7 days in the dorsal root ganglia and the neuron number was gradually increased after pyridoxine withdrawal.In addition,we also investigated the effects ofβ-NGF gene therapy on neuronal plasticity in pyridoxine-induced neuropathic dogs.To accomplish this,tyrosine kinase receptor A(TrkA),βIII-tubulin and doublecortin(DCX)immunohistochemical staining was performed at 3 days after the last pyridoxine treatment.TrkA-immunoreactive neurons were dramatically decreased in the pyridoxine group compared to the control group,but strong TrkA immunoreactivity was observed in the small-sized dorsal root ganglia in this group.TrkA immunoreactivity in the dorsal root ganglia was similar betweenβ-NGF and control groups.The numbers ofβⅢ-tubulin-and DCX-immunoreactive cells decreased significantly in the pyridoxine group compared to the control group.However,the reduction ofβⅢ-tubulin-and DCX-immunoreactive cells in the dorsal root ganglia in theβ-NGF group was significantly ameliorated than that in the pyridoxine group.These results indicate thatβ-NGF gene therapy is a powerful treatment of pyridoxine-induced neuropathic damage by increasing the TrkA and DCX levels in the dorsal root ganglia.The experimental protocol was approved by the Institutional Animal Care and Use Committee(IACUC)of Seoul National University,South Korea(approval No.SNU-060623-1,SNU-091009-1)on June 23,2006 and October 9,2009,respectively.

关键词： β-nerve growth factor βⅢ-tubulin doublecortin gene therapy neuron-glial antigen 2 neuropathy pyridoxine

Glucose metabolism and neurogenesis in the gerbil hippocampus after transient forebrain ischemia

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Neural Regeneration Research 2016年第8期11卷 1254-1259页

作者： Dae Young Yoo Kwon Young Lee Joon Ha Park Hyo Young Jung Jong Whi Kim Yeo Sung Yoon Moo-Ho Won Jung Hoon Choi In Koo Hwang department of Anatomy and Cell Biology College of Veterinary Medicineand Research Institute for Veterinary ScienceSeoul National University department of Anatomy College of Veterinary Medicine and Institute of Veterinary ScienceKangwon National University department of Neurobiology School of MedicineKangwon National University

Recent evidence exists that glucose transporter 3（GLUT3） plays an important role in the energy metabolism in the brain.Most previous studies have been conducted using focal or hypoxic ischemia models and have focused on changes in GLUT3 expression based on protein and m RNA levels rather than tissue levels.In the present study,we observed change in GLUT3 immunoreactivity in the adult gerbil hippocampus at various time points after 5 minutes of transient forebrain ischemia.In the sham-operated group,GLUT3 immunoreactivity in the hippocampal CA1 region was weak,in the pyramidal cells of the CA1 region increased in a time-dependent fashion 24 hours after ischemia,and in the hippocampal CA1 region decreased significantly between 2 and 5 days after ischemia,with high level of GLUT3 immunoreactivity observed in the CA1 region 10 days after ischemia.In a double immunofluorescence study using GLUT3 and glial-fibrillary acidic protein（GFAP）,we observed strong GLUT3 immunoreactivity in the astrocytes.GLUT3 immunoreactivity increased after ischemia and peaked 7 days in the dentate gyrus after ischemia/reperfusion.In a double immunofluorescence study using GLUT3 and doublecortin（DCX）,we observed low level of GLUT3 immunoreactivity in the differentiated neuroblasts of the subgranular zone of the dentate gyrus after ischemia.GLUT3 immunoreactivity in the sham-operated group was mainly detected in the subgranular zone of the dentate gyrus.These results suggest that the increase in GLUT3 immunoreactivity may be a compensatory mechanism to modulate glucose level in the hippocampal CA1 region and to promote adult neurogenesis in the dentate gyrus.

关键词： nerve regeneration transient forebrain ischemia glucose transporter 3 pyramidal cells astrocytes neuroblasts neural regeneration

Aldehyde dehydrogenase activity helps identify a subpopulation of murine adipose-derived stem cells with enhanced adipogenic and osteogenic differentiation potential

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World Journal of Stem Cells 2017年第10期9卷 179-186页

作者： Harumichi Itoh Shimpei Nishikawa Tomoya Haraguchi Yu Arikawa Shotaro Eto Masato Hiyama Toshie Iseri Yoshiki Itoh Munekazu Nakaichi Yusuke Sakai Kenji Tani Yasuho Taura Kazuhito Itamoto department of Small Animal Clinical science Joint Faculty of Veterinary Medicine Yamaguchi University department of veterinary Surgery Joint Faculty of Veterinary Medicine Yamaguchi University department of veterinary Radiology Joint Faculty of Veterinary Medicine Yamaguchi University department of veterinary Pathology Joint Faculty of Veterinary Medicine Yamaguchi University

AIM To identify and characterize functionally distinct subpopulation of adipose-derived stem cells(ADSCs).METHODS ADSCs cultured from mouse subcutaneous adipose tissue were sorted fluorescence-activated cell sorter based on aldehyde dehydrogenase(ALDH) activity, a widely used stem cell marker. Differentiation potentials were analyzed by utilizing immunocytofluorescece and its quantitative analysis.RESULTS Approximately 15% of bulk ADSCs showed high ALDH activity in flow cytometric analysis. Although significant difference was not seen in proliferation capacity, the adipogenic and osteogenic differentiation capacity was higher in ALDHHi subpopulations than in ALDHLo. Gene set enrichment analysis revealed that ribosome-related gene sets were enriched in the ALDHHi subpopulation. CONCLUSION High ALDH activity is a useful marker for identifying functionally different subpopulations in murine ADSCs. Additionally, we suggested the importance of ribosome for differentiation of ADSCs by gene set enrichment analysis.

关键词：脂肪质导出 stem/stromal 房间醛脱氢酶活动流动 cytometry Subpopulation 核糖体

The ubiquitin ligase Nedd4-2 mediates the regulation of PepT2 by mTORC1 in bovine mammary epithelial cells

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Animal Nutrition 2022年第3期10卷 12-18页

作者： Caihong Wang Fengqi Zhao Jianxin Liu Hongyun Liu College of Animal science Zhejiang UniversityHangzhou310058China department of Animal science and veterinary sciences University of VermontBurlingtonVT05405USA

Peptide transporter 2(PepT2)transports short peptides from the blood into bovine mammary epithelial cells(BMEC)to stimulate milk protein synthesis.Despite the fact that the effect of PepT2 is acknowledged in BMEC,little is known about its regulation.This study was completed to investigate the role of mammalian target of the rapamycin(mTOR)signaling in regulating the expression and function of PepT2 in BMEC.The regulation of PepT2 by mTOR in BMEC was studied in vitro using peptide transport assay,gene silencing,Western blot.The membrane expression of PepT2 and the uptake of b-Ala-Lys-N-7-amino-4-methylcoumarin-3-acetic acid(b-Ala-Lys-AMCA),a model dipeptide,in BMEC were reduced by rapamycin(a mTOR inhibitor)and silencing of either mTOR complex 1(mTORC1)or mTOR complex 2(mTORC2),stimulated by DEP domain-containing mTOR-interacting protein(DEPTOR,endogenous inhibitor of mTORC1 and mTORC2)silencing.The trafficking of PepT2 to the membrane and the uptake of b-Ala-Lys-AMCA was promoted by neuronal precursor cell-expressed developmentally down-regulated 4 isoform 2(Nedd4-2)silencing.The effects of knockdown of mTORC1,but not mTORC2,on cell membrane expression and transport activity of PepT2 was abolished by Nedd4-2 silencing.With immunofluorescence staining,PepT2 was identified to be interacting with Nedd4-2.The Nedd4-2 expression and the interaction between PepT2 and Nedd4-2 was increased through mTORC1 knockdown,indicating an increased ubiquitination of PepT2.The results revealed that mTORC1 can regulate the expression and function of PepT2 through Nedd4-2 in BMEC.

关键词： mTORC1 mTORC2 Nedd4-2 PepT2 Peptide uptake BMEC

Lactobacillus rhamnosus GR-1 attenuates foodborne Bacillus cereus-induced NLRP3 inflammasome activity in bovine mammary epithelial cells by protecting intercellular tight junctions

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Journal of Animal science and Biotechnology 2023年第1期14卷 307-321页

作者： Qiang Shan Ning Liu Xue Wang Yaohong Zhu Jinhua Yin Jiufeng Wang department of veterinary Clinical sciences College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China. College of Animal science and Technology Tarim UniversityAlar 843300China.

Background:Bacillus cereus is an important pathogen that causes human food poisoning,specifically diarrhea and vomiting.B.cereus can also induce mastitis in dairy cows and has a strong survival ability in milk,as it cannot be inactivated by high-temperature short-time pasteurization.Therefore,B.cereus can enter the market through pasteurized milk and other dairy products,imposing enormous hidden dangers on food safety and human health.Results:In this study,B.cereus 2101(BC)was isolated from milk samples of cows with mastitis.BC grew rapidly with strong hemolysis,making it difficult to prevent mastitis and ensure food security.MAC-T cells were treated with BC and/or Lactobacillus rhamnosus GR-1(LGR-1).Pretreatment with LGR-1 protected the integrity of tight junctions and the expression of zonula occludens-1(ZO-1)and occludin destroyed by BC.Furthermore,LGR-1 pretreatment reduced the expression of NOD-like receptor family member pyrin domain-containing protein 3(NLRP3),caspase recruitment and activation domain(ASC),Caspase-1 p20,gasdermin D(GSDMD)p30,inflammatory factors(interleukin(IL)-1βand IL-18),and cell death induced by BC.Moreover,LGR-1 pretreatment reduced NLRP3 inflammasome activity and increased expressions of ZO-1 and occludin induced by lipopolysaccharides(LPS)+ATP stimulation.MAC-T cells were transfected with NLRP3 si RNA or MCC950 and/or treated with BC and/or LGR-1.NLRP3-si RNA transfection and MCC950 attenuated BC-induced NLRP3 inflammasome activity.Expression of inflammatory cytokines and cell death suggested that the inflammatory pathway might play an important role in the induction of the NLRP3 inflammasome by BC and the protection of LGR-1.Conclusions:These results suggest that LGR-1 might be a probiotic alternative to antibiotics and could be administered to prevent mastitis in dairy cows,thus ensuring food security.

关键词： Bacillus cereus Intercellular tight junctions Lactobacillus rhamnosus GR-1 NLRP3 inflammasome

In-depth analysis of the genome of Trypanosoma evansi, an etiologic agent of surra

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science China(Life sciences) 2019年第3期62卷 406-419页

作者： Lili Zheng Ning Jiang Xiaoyu Sang Naiwen Zhang Kai Zhang Hongyu Chen Na Yang Ying Feng Ran Chen Xun Suo Qijun Chen Key Laboratory of Zoonosis College of Animal Science and Veterinary Medicine Shenyang Agricultural University department of Food science Shenyang Agricultural University department of Parasitology College of Veterinary Medicine Chinese Agricultural University

Trypanosoma evansi is the causative agent of the animal trypanosomiasis surra, a disease with serious economic burden worldwide. The availability of the genome of its closely related parasite Trypanosoma brucei allows us to compare their genetic and evolutionarily shared and distinct biological features. The complete genomic sequence of the T. evansi YNB strain was obtained using a combination of genomic and transcriptomic sequencing, de novo assembly, and bioinformatic analysis. The genome size of the T. evansi YNB strain was 35.2 Mb, showing 96.59% similarity in sequence and 88.97% in scaffold alignment with T. brucei. A total of 8,617 protein-coding genes, accounting for 31% of the genome, were predicted. Approximately 1,641 alternative splicing events of 820 genes were identified, with a majority mediated by intron retention, which represented a major difference in post-transcriptional regulation between T. evansi and T. brucei. Disparities in gene copy number of the variant surface glycoprotein, expression site-associated genes, microRNAs, and RNA-binding protein were clearly observed between the two parasites. The results revealed the genomic determinants of T. evansi, which encoded specific biological characteristics that distinguished them from other related trypanosome species.

关键词： Trypanosoma trypanosomiasis genome annotation function