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Study on Distribution of Four Pseudomonas Species in Living Environment Using Multiplex PCR

Open Journal of Stomatology 2024年第2期14卷 77-86页

作者： Sachiyo Hayashi Koji Umezawa Osamu Tsuzukibashi Akira Fukatsu Mana Fuchigami Chiaki Komine Hiroshi Yamamoto Mio Hagiwara-Hamano Yukiko Iizuka Satoshi Uchibori Masanobu Wakami Hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Takato Nomoto department of special needs dentistry Nihon University School of Dentistry at Matsudo Chiba Japan department of Laboratory Medicine for dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan department of Oral Surgery Nihon University School of Dentistry at Matsudo Chiba Japan department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.

关键词： Genus Pseudomonas Multiplex PCR Living Environment

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牙齿修复新纪元:原位生长

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Science Bulletin 2024年第1期69卷 17-21页

作者：邓菁菁于唯一卫彦邓旭亮 Beijing Laboratory of Biomedical Materials Department of Geriatric DentistryPeking University School and Hospital of StomatologyBeijing 100081China

Suffering from various pathological risks in daily life,such as cariogenic bacteria,traumatic force,and extreme pH values,tooth enamel defect has a stunning global prevalence of over 85%.Even worse,the enamel defect in most vertebrates cannot be self-regenerated to restore both exquisite hierarchical structures and exceptional mechanical properties throughout their lifetime[1].As such,a series of esthetic and functional complications caused by enamel defects exist persistently,such as dysfunction of the oral and maxillofacial system,reduced chewing efficiency,dentine hypersensitivity,periodontitis,*** and sustainable enamel repair techniques are urgently needed to improve life quality and ease the financial burden[2].

关键词： enamel exceptional defect

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Study on myocardial infarction based on nationwide inpatient sample database:a bibliometric analysis from 2000 to 2022

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Emergency and Critical Care Medicine 2024年第1期4卷 22-27页

作者： Tianyi Zhang Xue Yang Qian Zhuang Yuhua Liu Huajuan Bai Jianchao Liu Zhouheng Ye Institution of Hospital Management Department of Medical Innovation and ResearchChinese PLA General HospitalBeijingChina Outpatient department The Second Medical Center and National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalBeijingChina department of special Operations Medicine The Sixth Medical CenterChinese PLA General HospitalBeijingChina.

Background:Studies on myocardial infarction(MI)based on large medical databases have become popular in recent *** influence of the National Inpatient Sample(NIS),the largest collection of administrative healthcare data across the United States,on the field of MI has not been well *** study aimed to quantify the contribution of NIS to MI research using bibliometric ***:We searched the Web of Science Core Collection database to identify publications on MI using NIS from 2000 to *** indicators,such as the number of publications,citations,and Hirsch index(H-index),were summarized by years,authors,organizations,and *** and CiteSpace software were used to analyze the keywords and trends of the hot ***:A total of 342 articles on MI based on NIS were included.A significant growth in outputs related to MI using the NIS from 2000 to 2020 was *** publications were mainly from the United *** Mayo Clinic was the most prolific institution and had the most citations and the highest *** American Journal of Cardiology ranked first among journals with the highest number of publications,citations,and *** and healthcare management are the main focuses of this *** risks and care are receiving increased ***:This study suggests that NIS significantly contributes to high-quality output in MI *** efforts are needed to improve the impact of knowledge gained from the NIS on MI.

关键词： Administrative healthcare database Bibliometrics Big data Myocardial infarction National Inpatient Sample

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牙齿修复新纪元:原位生长（英文）

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Science Bulletin 2024年第1期 17-21页

作者：邓菁菁于唯一卫彦邓旭亮 Beijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology

Suffering from various pathological risks in daily life,such as cariogenic bacteria,traumatic force,and extreme pH values,tooth enamel defect has a stunning global prevalence of over 85%.Even worse,the enamel defect in most vertebrates cannot be self-regenerated to restore both exquisite hierarchical structures and exceptional mechanical properties throughout their lifetime [1].As such,a series of esthetic and functional complications caused by enamel defects exist persistently,such as dysfunction of the oral and maxillofacial system,reduced chewing efficiency,dentine hypersensitivity,periodontitis,*** and sustainable enamel repair techniques are urgently needed to improve life quality and ease the financial burden [2].

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Cell membrane vesicles derived from hBMSCs and hUVECs enhance bone regeneration

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Bone Research 2024年第2期12卷 361-371页

作者： Dandan Wang Yaru Guo Boon Chin Heng Xuehui Zhang Yan Wei Ying He Mingming Xu Bin Xia Xuliang Deng department of Pediatric dentistry Peking University School and Hospital of StomatologyBeijing 100081P.R.China department of geriatric dentistry Peking University School and Hospital of StomatologyNational Center for StomatologyNational Clinical Research Center for Oral DiseasesNational Engineering Research Center of Oral Biomaterials and Digital Medical DevicesBeijing 100081P.R.China department of Dental Materials&Dental Medical Devices Testing Center Peking University School and Hospital of StomatologyNational Engineering Research Center of Oral Biomaterials and Digital Medical DevicesBeijing 100081P.R.China

Bone tissue renewal can be enhanced through co-transplantation of bone mesenchymal stem cells(BMSCs)and vascular endothelial cells(ECs).However,there are apparent limitations in stem cell-based therapy which hinder its clinic ***,we investigated the potential of alternative stem cell substitutes for facilitating bone *** this study,we successfully prepared cell membrane vesicles(CMVs)from BMSCs and *** results showed that BMSC-derived cell membrane vesicles(BMSC-CMVs)possessed membrane receptors involved in juxtacrine signaling and growth factors derived from their parental ***-derived cell membrane vesicles(EC-CMVs)also contained BMP2 and VEGF derived from their parental ***-CMVs enhanced tube formation and migration ability of hUVECs,while EC-CMVs promoted the osteogenic differentiation of hBMSCs in *** a rat skull defect model,we found that co-transplantation of BMSC-CMVs and EC-CMVs could stimulate angiogenesis and bone formation in ***,our research might provide an innovative and feasible approach for cell-free therapy in bone tissue regeneration.

关键词： hinder apparent vesicles

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Identification of tumor-suppressor genes in lung squamous cell carcinoma through integrated bioinformatics analyses

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Oncology Research 2024年第1期32卷 187-197页

作者： HENG LI YOUMING LEI GAOFENG LI YUNCHAO HUANG The 2nd department of Thoracic Surgery The Third Affiliated Hospital of Kunming Medical UniversityYunnan Tumor HospitalKunming650118China The 1st department of Thoracic Surgery The Third Affiliated Hospital of Kunming Medical UniversityYunnan Tumor HospitalKunming650118China department of geriatric Thoracic Surgery The First Affiliated Hospital of Kunming Medical UniversityKunming650032China

Lung cancer is a prevalent malignancy,and fatalities of the disease exceed 400,000 cases *** squamous cell carcinoma(LUSC)has been recognized as the most common pathological form of lung *** comprehensive understanding of molecular features related to LUSC progression has great significance in LUSC prognosis assessment and clinical *** this study,we aim to identify a panel of signature genes closely associated with LUSC,which can provide novel insights into the progression of *** expression profiles were retrieved from public resources including gene expression omnibus(GEO)and the cancer genome atlas(TCGA)*** expressed genes(DEGs)between LUSC specimens and normal lung tissues were identified by bioinformatics analyses.A total of 66 DEGs were identified based on two cohorts of *** plugin of Cytoscape software was utilized for the further analyses of the top 10 candidate hub genes including OGN,ABI3BP,MAMDC2,FGF7,FAM107A,SPARCL1,DCN,COL14A1,and MFAP4 and CHRDL1,which showed significant downregulation in *** LUSC cell lines were used to validate the functions of CHRDL1 and FAM107A through overexpression ***,our data revealed novel candidate tumor-suppressor genes in LUSC,suggesting previously unappreciated mechanisms in the progression of LUSC.

关键词： LUSC DEGs WGCNA PPI network Tumor-suppressor

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The Oral Health Status of Person with special needs in Kumasi

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Open Journal of Stomatology 2024年第1期14卷 14-31页

作者： Ama Agyeibea Amuasi Alexander Oti-Acheampong Victoria N. A. Boadu Lord J. J. Gowans The department Child Oral Health & Orthodontics School of Medicine and Dentistry College of Health Sciences Kwame Nkrumah University of Science and Technology Kumasi Ghana The department of Oral & Maxillofacial Sciences School of Medicine and Dentistry College of Health Sciences Kwame Nkrumah University of Science and Technology Kumasi Ghana The department of Community dentistry School of Medicine and Dentistry College of Health Sciences Kwame Nkrumah University of Science and Technology Kumasi Ghana

Background: The most prevalent non-communicable disease worldwide is oral health-related disease, with dental caries and periodontal conditions being common. Oral health status significantly impacts overall health and quality of life. Barriers to dental care affect children with special needs. This study evaluates the oral health status and treatment requirements of people with special needs. Methodology: This descriptive cross-sectional study in Kumasi, Ghana examined oral health status among people, 121 children with special needs over two months, in June-July 2022. Dental caries, periodontal changes, malocclusion, and trauma were assessed. The data was analyzed using SPSS 20.0. Results: The oral health status among people with special needs in this study was generally moderate, with a range from very good to very poor. The prevalence of dental caries was 37%, with a mean DMFT score of 2.82. Periodontal health showed unsatisfactory indicators such as mobile teeth, poor gingival health, high plaque scores, and the presence of halitosis. Conclusion: This descriptive study aimed to provide baseline data on the oral health status of special needs students in Kumasi, addressing the lack of knowledge in this area. It highlighted the importance of dental care in maintaining good oral health and overall well-being in individuals with special needs. Coordinated efforts by dental professionals are needed to provide dental health education and preventive interventions for these children.

关键词： special needs Oral Health Dental Caries Periodontal Diseases Malocclusion

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Next-generation sequencing reveals relapse and leukemia-free survival risks in newly diagnosed acute myeloid leukemia treated with CAG regimen combined with decitabine

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Cancer Pathogenesis and Therapy 2024年第2期2卷 112-120页

作者： Sai Huang Peng Chen Lu Wang Lingmin Xu Nan Wang Fei Li Liping Dou Daihong Liu department of Hematology Senior Department of HematologyThe Fifth Medical Center of PLA General HospitalBejing 100039China National Clinical Research Center of geriatric Diseases Chinese PLA General HospitalBejing 100853China Chinese PLA Medical School Beijing 100853China department of Hematology The First Medical Center of PLA General HospitalBejing 100853China

Background:Acute myeloid leukemia(AML)is a heterogeneous hematopoietic malignancy whose prognosis is associated with several ***,a deoxyribonucleic acid(DNA)methyltransferase(DNMT)in-hibitor,combined with cytarabine,aclarubicin hydrochloride,and granulocyte colony-stimulating factor(DCAG),has been used in patients newly diagnosed with *** regimen has been especially used in older and fragile patients who are immunocompromised or have co-morbidities,as well as those with specific gene ***,the integration of molecular risk stratification and treatment guidance for the DCAG regimen has not been well ***,this study aimed to investigate the genetic mutations associated with AML and establish appropriate treatment strategies for patients newly diagnosed with ***:This study analyzed the clinical data and genetic mutations based on next-generation sequencing(NGS)in 124 newly diagnosed patients with AML who received the DCAG regimen at the People's Liberation Army(PLA)General Hospital from January 2008 to August *** associated with the cumulative incidence of relapse(CIR)and leukemia-free survival(LFS)in patients newly diagnosed with AML were ***:The most adverse prognosis of DCAG-treated patients was observed in those with FLT3-ITD,KIT,PTPN11,GATA2,or IDH1 mutations during univariable analysis,whereas PTPN11 mutation was solely significant in multivariable analysis,with an increased likelihood of CIR(P=0.001)and reduced LFS duration(P=0.077).Hyperleukocytosis was maintained as an independent risk factor for increased CIR risk(P=0.044)and decreased LFS duration(P=0.042)in multivariable *** this study,we validated the risk classification of patients with AML receiving an epigenetic modifier-based induction regimen across a broad age ***:NGS demonstrated a dismal overall outcome in patients with the rare PTPN11 mutations,indicating the need for new therapies that target this h

关键词： Acute myeloid leukemia Next-generation sequencing Prognosis DNA methyltransferase Chemotherapy

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Expert consensus on difficulty assessment of endodontic therapy

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International Journal of Oral Science 2024年第1期16卷 15-25页

作者： Dingming Huang Xiaoyan Wang Jingping Liang Junqi Ling Zhuan Bian Qing Yu Benxiang Hou Xinmei Chen Jiyao Li Ling Ye Lei Cheng Xin Xu Tao Hu Hongkun Wu Bin Guo Qin Su Zhi Chen Lihong Qiu Wenxia Chen Xi Wei Zhengwei Huang Jinhua Yu Zhengmei Lin Qi Zhang Deqin Yang Jin Zhao Shuang Pan Jian Yang Jiayuan Wu Yihuai Pan Xiaoli Xie Shuli Deng Xiaojing Huang Lan Zhang Lin Yue Xuedong Zhou State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&department of Operative dentistry and Endodontics West China Hospital of StomatologySichuan UniversityChengduChina department of Cariology and Endodontology Peking University School and Hospital of Stomatology&National Center of Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology&Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health&NMPA Key Laboratory for Dental MaterialsBeijingChina department of Endodontics Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Clinical Research Center for Oral DiseasesNational Center for StomatologyShanghai Key Laboratory of StomatologyShanghaiChina department of Operative dentistry and Endodontics Hospital of StomatologyGuanghuaSchool of StomatologySun Yat-Sen University&Guangdong Provincial Key Laboratory of StomatologyGuangzhouChina The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of StomatologyWuhan UniversityWuhanChina department of Operative dentistry&Endodontics School of StomatologyThe Fourth Military Medical UniversityXi’anChina department of Endodontics Beijing Stomatological HospitalSchool of StomatologyCapital Medical UniversityBeijingChina State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&department of Preventive dentistry West China Hospital of StomatologySichuan UniversityChengduChina State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&department of geriatric dentistry West China Hospital of Stomatology

Endodontic diseases are a kind of chronic infectious oral disease. Common endodontic treatment concepts are based on the removal of inflamed or necrotic pulp tissue and the replacement by gutta-percha. However, it is very essential for endodontic treatment to debride the root canal system and prevent the root canal system from bacterial reinfection after root canal therapy(RCT). Recent research, encompassing bacterial etiology and advanced imaging techniques, contributes to our understanding of the root canal system’s anatomy intricacies and the technique sensitivity of RCT. Success in RCT hinges on factors like patients, infection severity, root canal anatomy, and treatment techniques. Therefore, improving disease management is a key issue to combat endodontic diseases and cure periapical lesions. The clinical difficulty assessment system of RCT is established based on patient conditions, tooth conditions, root canal configuration, and root canal needing retreatment, and emphasizes pre-treatment risk assessment for optimal outcomes. The findings suggest that the presence of risk factors may correlate with the challenge of achieving the high standard required for RCT. These insights contribute not only to improve education but also aid practitioners in treatment planning and referral decision-making within the field of endodontics.

关键词： anatomy difficulty treatment

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Two cases on primary bone marrow lymphoma

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Cancer Pathogenesis and Therapy 2024年第1期2卷 58-61页

作者： Zining Wang Lu Sun Yue Wang Haoran Chen Hongbin Pu Bo Yang Xuechun Lu Medical School of Chinese PLA Beijing 100853China department of Hematology The Second Medical Center&National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalBeijing 100853China department of Pathology The First Medical CenterChinese PLA General HospitalBeijing 100853China National Institutes for Food and Drug Control Beijing 102629China Management School Shanxi Medical UniversityTaiyuanShanxi 030604China

Managing Editor:Peng Lyu Primary bone marrow lymphoma(PBML)is a rare lymphoma accounting for 1.23%of non-Hodgkin lymphoma(NHL)*** is characterized by tumor cells invading only the bone marrow without systemic lymphadenopathy or extranodal organ *** to the lack of standardized treatment regimens,most patients are treated using rituximab with cyclophosphamide,doxorubicin,vincristine,and prednisone(R-CHOP)regimens;however,their efficacy needs to be further investigated.

关键词： lymphoma cases involvement

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