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Individual stages of bacterial dichloromethane degradation mapped by carbon and chlorine stable isotope analysis

Journal of Environmental Sciences 2019年第4期31卷 147-160页

作者： Maria L.Torgonskaya Anatoly M.Zyakun Yuri A.Trotsenko Kestutis S.Laurinavichius Steffen Kümmel Stéphane Vuilleumier Hans H.Richnow Laboratory of Radioactive Isotopes G.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences Laboratory of Mass Spectrometry G.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences department of Isotope Biogeochemistry Helmholtz Centre for Environmental Research-UFZ Université de Strasbourg CNRS

The fractionation of carbon and chlorine stable isotopes of dichloromethane(CH_2Cl_2,DCM)upon dechlorination by cells of the aerobic methylotroph Methylobacterium extorquens DM4 and by purified DCM dehalogenases of the glutathione S-transferase family was *** effects for individual steps of the multi-stage DCM degradation process,including transfer across the cell wall from the aqueous medium to the cell cytoplasm,dehalogenase binding,and catalytic reaction,were *** observed carbon and chlorine isotope fractionation accompanying DCM consumption by cell supensions and enzymes was mainly determined by the breaking of C\Cl bonds,and not by inflow of DCM into *** isotope effects of DCM dehalogenation were initially masked in high density cultures,presumably due to inverse isotope effects of non-specific DCM oxidation under conditions of oxygen *** cofactor supply remarkably affected the correlation of variations of DCM carbon and chlorine stable isotopes(Δδ^(13)C/Δδ^(37)Cl),increasing corresponding ratio from 7.2–8.6 to 9.6–10.5 under conditions of glutathione *** suggests that enzymatic reaction of DCM with glutathione thiolate may involve stepwise breaking and making of bonds with the carbon atom of DCM,unlike the uncatalyzed reaction,which is a one-stage process,as shown by quantum-chemical modeling.

关键词： Dichloromethane(DCM) Microbial degradation Aerobic methylotrophic bacteria Dichloromethane dehalogenase Carbon and chlorine isotopic fractionation

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Relationship between Ethanol and 2-phenylethanol Stress Tolerance and Fatty Acid Compositions of Saccharomyces cerevisiae

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Journal of Food Science and Engineering 2013年第2期3卷 71-78页

作者： Olga Mameeva Valentin Podgorsky department of physiology of industrial microorganisms Zabolotny Institute of Microbiology and Virology of National Academy ofSciences of Ukraine Kiev D03680 Ukraine

In the present study, we investigated of the ethanol （Eth）, 2-phenylethanol （2-PE） and ethanol/2-phenylethanol （Eth/2-PE） stress tolerance and established relationship between stress tolerance and fatty acid compositions of wine yeast strain S. cerevisiae UCM Y-524 in relation to different cultivation factors. Changes in cell membrane fatty acid profiles studied under different temperature and media. The active oxygenation, semi-aerobic cultivation supplemented Tween 80 conditions were used in different combinations. The concentrations of 2-PE and Eth, the fatty acids methyl esters （FAMES） in the media were measured by GC-MS analyses. 2-PE, Eth, FAMES were identified by NIST02 MS database at the 2-PE and Eth standard solution （Merck, Germany）, bacterial FAMES standard （Supelco）. Palmitoleic and oleic acid were dominated for S. cerevisiae UCM Y-524. The unsaturated/saturated fatty acid （UFA/SFA） ratio was higher than five for S. cerevisiae UCM Y-524 at 14 ~C in simple medium and about more than three for other conditions. It has been found that yeast cells grown in the presence of the 2-PE, Eth and Eth/2-PE appear to increase the amount of unsaturated fatty acids, especially oleic acid, in cellular lipids especially under active oxygenation supplemented with Tween 80. S. cerevisiae UCM Y-524 has a higher tolerance under 2-PE, Eth and Eth/2-PE stress at 28 ~C in a simple medium under aerobic conditions. A higher concentration of unsaturated fatty acids in the membrane S. cerevisiae UCM Y-524 and a lower temperature under aerobic conditions, has been considered to have a positive influence on the excretion of 2-phenylethanol.

关键词： Yeasts 2-phenylethanol ethanol stress tolerance fatty acids.

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Mechanisms of phototherapy of Alzheimer’s disease during sleep and wakefulness:the role of the meningeal lymphatics

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Frontiers of Optoelectronics 2023年第3期16卷 57-76页

作者： Semyachkina‑Glushkovskaya Oxana Shirokov Alexander Blokhina Inna Fedosov Ivan Terskov Andrey Dubrovsky Alexander Tsoy Maria Elovenko Daria Adushkina Viktoria Evsukova Arina Telnova Valeria Tzven Anna Krupnova Valeria Manzhaeva Maria Dmitrenko Alexander Penzel Thomas Kurths Jürgen Institute of Physics Humboldt UniversityBerlin 12489Germany department of Biology Saratov State UniversitySaratov 410012Russia Institute of Biochemistry and physiology of Plants and microorganisms Russian Academy of SciencesSaratov 410049Russia Charité–Universitätsmedizin Berlin Berlin 10117Germany department of Complexity Scienc Potsdam Institute for Climate Impact ResearchPotsdam 14473Germany

With the increase in the aging population,the global number of people with Alzheimer’s disease(AD)progressively increased *** situation is aggravated by the fact that there is no the efective pharmacological therapy of ***(PBM)is non-pharmacological approach that has shown very promising results in the therapy of AD in pilot clinical and animal ***,the mechanisms of therapeutic efects of PBM for AD are poorly *** this study on mice,we demonstrate that photodynamic efects of 5-aminolevulenic acid and laser 635 nm cause reduction of network of the meningeal lymphatic vessels(MLVs)leading to suppression of lymphatic removal of beta-amyloid(Aβ)from the right lateral ventricle and the *** the original protocol of PBM under electroencephalographic monitoring of wakefulness and sleep stages in non-anesthetized mice,we discover that the 7-day course of PBM during deep sleep *** provides better restoration of clearance of Aβfrom the ventricular system of the brain and the *** results shed light on the mechanism of PBM and show the stimulating efects of PBM on the brain lymphatic drainage that promotes transport of Aβvia the lymphatic *** efects of PBM on the brain lymphatics in sleeping brain open a new niche in the study of restorative functions of sleep as well as it is an important informative platform for the development of innovative smart sleep technologies for the therapy of AD.

关键词： Alzheimer’s disease Photobiomodulation Brain lymphatics Sleep Mechanisms

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Interactive Effect of GA_3 ,N and P Ameliorate Growth, Seed and Fibre Yield by Enhancing Photosynthetic Capacity and Carbonic Anhydrase Activity of Linseed: A Dual Purpose Crop

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Journal of Integrative Agriculture 2013年第7期12卷 1183-1194页

作者： Mohammad N Khan Firoz Mohammad department of Biology Faculty of Science University of Tabuk Plant physiology Section Department of Botany Aligarh Muslim University

Linseed （Linum usitatissimum L.） is an important dual-purpose, industrial crop. Its seeds are used for the extraction of oil and stem for fibres. However, the production of linseed is not going parallel with the increasing demand of its products. The present work was carried out with an aim to find out whether exogenous application of gibberellic acid （GA3） with or without graded levels of nitrogen （N） and phosphorus （P） could improve the performance of three linseed genotypes Parvati, Shekhar and Shubhra together with minimizing the costly fertilizer input and losses. Four combinations of N and P, viz., 0 mg N＋0 mg P kg-1 soil （N0P0）, N13.4 P4.46 , N26.8P8.94 and N40.2P13.4 were constituted. Half dose of each combination was applied basally at the time of sowing and remaining half dose was given at 40 d after sowing （DAS） as foliar spray along with 10-6 mol L-1 GA3 . Prior to sowing, the seeds of each linseed genotype were grouped in to two, one group of seeds was soaked in 0 mol L-1 GA3 （control） and the other group was soaked in 10-6 M GA3 solution, each for 8 hours. Treatments were comprised of （i） 0 mol L-1 GA3＋N0P0 （T0 , control）, （ii） 10-6 mol L-1 GA3 ＋ N13.4P4.46 （T1）, （iii） 10-6 mol L-1 GA 3 ＋N 26.8 P 8.94 （T2） and （iv） 10-6 mol L-1 GA3＋N40.2P13.4 （T3）. The crop performance was assessed in terms of growth, physiological and biochemical parameters at 60 and 75 DAS and yield attributes at harvest （175 DAS）. The results showed a parallel increase in most of the parameters with increasing levels of N and P. However, application of 10-6 mol L-1 GA3 in association with N26.8P8.94 proved best, it enhanced seed yield, oil yield and fibre yield by 83.3, 97.3 and 78.7%, respectively accompanied with increase in net photosynthetic rate, carbonic anhydrase activity and dry matter accumulation. Among the genotypes tested, Shubhra performed best, while Parvati the least for most of the parameters studied. Thus, combined application of 10-6 mol L-1 GA3 plus N26.8P8.94 pro

关键词： gibberellin photosynthesis crbonic anhydrase linseed

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Energy model based on solar potential and the production of electrical energy through living plants’photosynthesis

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Clean Energy 2024年第3期8卷 116-126页

作者： Raymond Daniel Rodriguez Martinez department of industrial Production Faculty of EngineeringUniversidad Metropolitana1073 CaracasVenezuela

Developing a sustainable energy model is imperative considering the current trend towards decarbonizing sectors *** this purpose,Venezuela was used as a reference to propose an energy model focused on taking advantage of plant photosynthesis through microbial-vegetable fuel cells together with an agro-photovoltaic system to enhance energy and agricultural *** production from the cells was estimated using an average power density of 264 mW/m^(2) over 4%of the areas destined for crops in the entire Venezuelan region,obtaining an annual production of 19.889 GWh/*** contrast,the energy production of the agro-photovoltaic system was modelled using PVsyst software on 50%of the area used for the cells distributed throughout the states of Anzoátegui,Guárico,Monagas and Portuguesa according to their meteorological conditions,solar irradiation and agricultural activity,resulting in 3703417 GWh/*** resulting whole system proved to be able to cover>10 times the installed electricity generation capacity at a national level and,together with the tremendous scalability of the microbial fuel cells,it shows that Venezuela has a high potential for the production and distribution of clean energy.

关键词： ecosystems microbial fuel cell microorganisms renewable sustainability

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Doxycycline blocks gastric ulcer by regulating matrix metalloproteinase-2 activity and oxidative stress

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World Journal of Gastroenterology 2011年第28期17卷 3310-3321页

作者： Laishram Pradeepkumar Singh Amartya Mishra Debjit Saha Snehasikta Swarnakar department of physiology Drug Development Diagnostics and Biotechnology DivisionIndian Institute of Chemical Biology

AIM: To examine the effect of doxycycline on the activity of matrix metalloproteinases (MMPs) and oxidative stress in gastric tissues of rats following gastric ***: Gastric ulcers were generated in rats by administration of 70% ethanol,and activity of doxycycline was tested by administration 30 min prior to ***,the effect of doxycycline was tested in an indomethacin-induced gastric ulcer *** activities and expression of MMPs were examined by zymography and Western blot ***: Gastric injury in rats as judged by elevated ulcer indices following exposure to ulcerogen,either indomethacin or ethanol,was reversed significantly by ***-induced ulcerated gastric tissues exhibited about 12-fold higher proMMP-9 activity and about 5-fold higher proMMP-3 activity as compared to control ***,ethanol induced about 22-fold and about 6-fold higher proMMP-9 and proMMP-3 activities,respectively,in rat gastric *** proMMP-9 and MMP-3 activities were markedly decreased by doxycycline in ulcerogen treated rat gastric *** contrast,the reduced MMP-2 activity in ulcerated tissues was increased by doxycycline during ulcer *** the other hand,doxycycline inhibited significantly proMMP-9,-2 and -3 activities in *** addition,doxycycline reduced oxidative load in gastric tissues and scavenged H2O2 in *** results suggest a novel regulatory role of doxycycline on MMP-2 activity in addition to inhibitory action on MMP-9 and MMP-3 during prevention of gastric ***: This is the first demonstration of dual action of doxycycline,that is,regulation of MMP activity and reduction of oxidative stress in arresting gastric injury.

关键词： Doxycycline Extracellular matrix Matrix metalloproteinases Reactive oxygen species Tissue inhibitor of metalloproteinase

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Evaluation of the anti-diabetic properties of Mucuna pruriens seed extract

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Asian Pacific Journal of Tropical Medicine 2011年第8期4卷 632-636页

作者： Stephen O Majekodunmi Ademola A Oyagbemi Solomon Umukoro Oluwatoyin A Odeku department of Pharmaceutics and Pharmaceutical Technology Faculty of PharmacyUniversity of UyoUyoNigeria department of Pharmaceutics and industrial Pharmacy Faculty of PharmacyUniversity of IbadanIbadanNigeria department of Veterinary physiology Biochemistry & PharmacologyUniversity of IbadanNigeria department of Pharmacology and Therapeutics College of MedicineUniversity of IbadanIbadanNigeria

Objective:To explore the antidiabetic properties of Mucuna pruriens(***).Methods: Diabetes was induced in Wistar rats by single intravenous injection of 120 mg/kg of alloxan monohydrate and different doses of the extract were administered to diabetic *** blood glucose level was determined using a glucometer and results were compared with normal and untreated diabetic *** acute toxicity was also determined in albino ***:Results showed that the administration of 5,10,20,30,40,50,and 100 mg/kg of the crude ethanolic extract of *** seeds to alloxan-induced diabetic rats(plasma glucose > 450 mg/dL) resulted in 18.6%,24.9%,30.8%,41.4%,49.7%,53.1%and 55.4%reduction,respectively in blood glucose level of the diabetic rats after 8h of treatment while the administration of glibenclamide (5 mg/kg/day) resulted in 59.7%*** administration of the extract resulted in a significant dose dependent reduction in the blood glucose level(P<0.001).It also showed that the antidiabetic activity of *** seeds resides in the methanolic and ethanolic fractions of the *** toxicity studies indicated that the extract was relatively safe at low doses,although some adverse reactions were observed at higher doses(8-32 mg/kg body weight),no death was ***,oral administration of *** seed extract also significandy reduced the weight loss associated with ***:The study clearly supports the traditional use of *** for the treatment of diabetes and indicates that the plant could be a good source of potent antidiabetic drug.

关键词： Antidiabetic Mucuna pruriens Hypoglycemic activity Toxicity Diabetes mellitus

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The relationship among amyloid-βdeposition,sphingomyelin level,and the expression and function of P-glycoprotein in Alzheimer’s disease pathological process

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Neural Regeneration Research 2023年第6期18卷 1300-1307页

作者： Zi-Kang Xing Li-Sha Du Xin Fang Heng Liang Sheng-Nan Zhang Lei Shi Chun-Xiang Kuang Tian-Xiong Han Qing Yang State Key Laboratory of Genetic Engineering School of Life SciencesShanghai Engineering Research Center of Industrial MicroorganismsFudan UniversityShanghaiChina Shanghai Key Lab of Chemical Assessment and Sustainability School of Chemical Science and EngineeringTongji UniversityShanghaiChina department of Traditional Chinese Medicine Tenth People’s Hospital of Tongji UniversityShanghaiChina

In Alzheimer’s disease,the transporter P-glycoprotein is responsible for the clearance of amyloid-βin the ***-βcorrelates with the sphingomyelin metabolism,and sphingomyelin participates in the regulation of *** amyloid cascade hypothesis describes amyloid-βas the central cause of Alzheimer’s disease *** understanding of the change of P-glycoprotein and sphingomyelin along with amyloid-βand their potential association in the pathological process of Alzheimer’s disease is ***,we found that the expression of P-glycoprotein in APP/PS1 mice tended to increase with age and was significantly higher at 9 and 12 months of age than that in wild-type mice at comparable *** functionality of P-glycoprotein of APP/PS1 mice did not change with age but was significantly lower than that of wild-type mice at 12 months of *** sphingomyelin levels,increased ceramide levels,and the increased expression and activity of neutral sphingomyelinase 1 were observed in APP/PS1 mice at 9 and 12 months of age compared with the levels in wild-type *** results were observed in the Alzheimer’s disease mouse model induced by intracerebroventricular injection of amyloid-β1-42 and human cerebral microvascular endothelial cells treated with amyloid-β*** human cerebral microvascular endothelial cells,neutral sphingomyelinase 1 inhibitor interfered with the changes of sphingomyelin metabolism and P-glycoprotein expression and functionality caused by amyloid-β1-42 *** sphingomyelinase 1 regulated the expression and functionality of P-glycoprotein and the levels of sphingomyelin and ***,these findings indicate that neutral sphingomyelinase 1 regulates the expression and function of P-glycoprotein via the sphingomyelin/ceramide *** studies may serve as new pursuits for the development of anti-Alzheimer’s disease drugs.

关键词： Alzheimer’s disease amyloid-β APP/PS1 mice ceramide ezrin-radixin-moesin human cerebral microvascular endothelial cells neutral sphingomyelinase 1 P-glycoprotein sphingomyelin synthase sphingomyelin

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SUPPRESSIVE EFFECT OF STRESS ON THE RELEASE OF HYDROGEN PEROXIDE FROM RAT MACROPHAGES

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Chinese Science Bulletin 1989年第4期34卷 340-345页

作者：范少光丁玄宙丁桂凤韩济生 department of physiology Beijing Medical University department of Immunology Beijing Medical University

It is known that exposure to stress reduces the activity of T lymphocytes and natural killer cells. The effect of stress on B lymphocyte is more complicated. It may reduce or enhance the activity, depending on the nature and intensity of the stimulus, as well as the biological characteristics of the anima. Macrophages are versatile cells which play a central role in the stimulation and expression of immune system. Hydrogen peroxide （H2O2）, superoxide anion and hydroxyl radicals produced by macrophages are involved in the killing of microorganisms and tumor cells.

关键词： stress macrophage hydrogen peroxide β-endorphin naltrexone.

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New Extracellular Bacillus subtilis Lectins with Sialic Acid-Specificity

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Journal of Agricultural Science and Technology(A) 2014年第6期4卷 541-546页

作者： Valentin Podgorsky Emma Kovalenko Iryna Karpova Elena Sashchuk Ekaterina Getman department of physiology of industrial microorganisms Zabolotny Institute of Microbiology and Virology National Academy ofSciences of Ukraine Kyiv D03680 Ukraine department of Human Genetics Institute of Molecular Biology and Genetics NatiGnal Academy of Sciences of Ukraine KyivD03143 Ukraine

The purpose of this work is to perform a detailed study of carbohydrate specificity of the new extracellular bacilli lectins which is considered to determine mechanisms of the lectins action. Sources of lectins were bacterial strains from Ukrainian collection of microorganisms. The optimized protocol of bacilli lectins isolation and purification included precipitation with ammonium sulfate with subsequent gel filtration chromatography on Sepharose CL-6B. Hemagglutinating activity of bacilli lectins and their fine carbohydrate specificity to sialic acids and their derivatives as well as sialic asid-containing and asialic glycoconjugates were studied. The ability of extracellular bacilli lectins to discriminate a- and 13-conformation of carbohydrate molecule and the type of connection between the monomers was determined. Studied lectins showed the most affinity to glycoconjugates containing both types of sialic acids （N-acetylneuraminic acid （Neu5Ac） and N-glycolylneuraminic acid （NeuGc）） and it is supposed to be a basis of their diagnostic and analytical potential.

关键词： Bacillus subtilis lectins sialic acids specificity.

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