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Disruption of interstitial cells of Cajal networks after massive small bowel resection

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World Journal of Gastroenterology 2013年第22期19卷 3415-3422页

作者： Jie Chen Lei Du Yong-Tao Xiao Wei Cai department of pediatric surgery Xin Hua Hospital Shanghai Jiaotong University School of Medicine department of pediatric surgery School of Medicine Affiliated Hospital of Nantong University Shanghai Key laboratory of pediatric Gastroenterology and Nutrition Shanghai Institute of Pediatric Research Shanghai Jiaotong University

AIM: To investigate the disruptions of interstitial cells of Cajal (ICC) in the remaining bowel in rats after massive small bowel resection (mSBR). METHODS: Thirty male Sprague-Dawley rats fitting entry criteria were divided randomly into three experimental groups (n = 10 each): Group A rats underwent bowel transection and re-anastomosis (sham) and tissue samples were harvested at day 7 post-surgery. Group B and C rats underwent 80% small bowel resection with tissue harvested from Group B rats at day 7 post-surgery, and from Group C rats at day 14 postsurgery. The distribution of ICC at the site of the resid-ual small bowel was evaluated by immunohistochemical analysis of small intestine samples. The ultrastructural changes of ICC in the remnant ileum of model rats 7 and 14 d after mSBR were analyzed by transmission electron microscopy. Intracellular recordings of slow wave oscillations were used to evaluate electrical pacemaking. The protein expression of c-kit, ICC phenotypic markers, and membrane-bound stem cell factor (mSCF) in intestinal smooth muscle of each group were detected by Western blotting. RESULTS: After mSBR, immunohistochemical analysis indicated that the number of c-kit-positive cells was dramatically decreased in Group B rats compared with sham tissues. Significant ultrastructural changes in ICC with associated smooth muscle hypertrophy were also observed. Disordered spontaneous rhythmic contractions with reduced amplitude (8.5 ± 1.4 mV vs 24.8 ± 1.3 mV, P = 0.037) and increased slow wave frequency (39.5 ± 2.1 cycles/min vs 33.0 ± 1.3 cycles/min, P = 0.044) were found in the residual intestinal smooth muscle 7 d post mSBR. The contractile function and electrical activity of intestinal circular smooth muscle returned to normal levels at 14 d post mSBR (amplitude, 14.9 ± 1.6 mV vs 24.8 ± 1.3 mV; frequency, 30.7 ± 1.7 cycles/min vs 33.0 ± 1.3 cycles/min). The expression of Mscf and c-kit protein was decreased at 7 d (P = 0.026), but gradually returne

关键词： Interstitial cells of Cajal c-kit Slow wave Massive small bowel resection Intestinal dysfunction

Autologous Transplantation for Parkinson's Disease Patients:Feasibility and Challenge

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Neuroscience Bulletin 2021年第7期37卷 1085-1086页

作者： Qi Zhang Yanlin Wang Yukai Wang Han Liu Huifang Sun Zhuoya Wang Changhe Shi Jing Yang Yuming Xu department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Academy of Medical Sciences Zhengzhou UniversityZhengzhou 450052China State Key laboratory of stem cell and Reproductive biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for stem cell and Regeneration Chinese Academy of SciencesBeijing 100101China department of National stem cell Resource Center Institute of ZoologyChinese Academy of SciencesBeijing 100101China Beijing Institute for stem cell and Regenerative Medicine Beijing 100101China

Parkinson’s Disease(PD),second only to Alzheimer’s disease,is a neurodegenerative disease,most commonly occurring in people over the age of 65 years and is mostly caused by loss of dopamine neurons[1].Clinically,motor symptoms such as resting tremor,motor retardation,muscular rigidity,and disturbance of postural balance are the main symptoms,followed by non-motor symptoms such as cognitive impairment,autonomic nervous system dysfunction,depression,and sleep disorder[2].In 2016,>6.1 million people were affected with PD globally,2.4 times the number in *** large number of affected people,coupled with the high mortality and disability rates,has placed a great burden on society[3].Traditional treatment methods mainly include drugs and surgery and are supplemented by physical therapy.

关键词： drugs mortality Clinical

All-trans retinoic acid inhibits the malignant behaviors of hepatocarcinoma cells by regulating ferroptosis

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Genes & Diseases 2022年第6期9卷 1742-1756页

作者： Yanting Sun Yun He Jishuang Tong Daijiang Liu Haodong Zhang Tongchuan He Yang Bi stem cell biology and Therapy laboratory Children’s Hospital of Chongqing Medical UniversityNational Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChongqing Key Laboratory of PediatricsChongqing 400014PR China department of pediatric surgery Children’s Hospital of Chongqing Medical UniversityChongqing 400014PR China department of Gastroenterology Chongqing Emergency Medical CentreChongqing 400014PR China Molecular Oncology laboratory Department of SurgeryThe University of Chicago Medical CentreChicagoIL 60637USA

All-trans retinoic acid(ATRA)can reverse the malignant behaviors of hepatocellular carcinoma(HCC)cells,thereby exerting anti-HCC effect;however,the underlying mechanism is yet to be *** study aimed to demonstrate that ATRA is vital to ferroptosis in ***-related genes exhibit different expression in patients with HCC compared to that in healthy individuals.A total of 20 amino acid products were detected in HepG2 cells,the expression level of 5 was decreased after ATRA *** improved the levels of lipid ROS,MDA,and NAPDt/NADPH,and reduced the mt-DNA copy number and changed the structure of mitochondria,in HepG2 and Hep3B *** found the expression of genes positively correlated with ferroptosis to increase and those negatively correlated to decrease with ATRA *** of ferroptosis by Ferrostatin-1 reversed ATRA-inhibited proliferation of HCC cells,along with cell migration and *** synthesis was blocked by ATRA,accompanied by decreased cystine content and increased glutamate content,and downregulation of the expression of GSH synthesis-related *** findings suggested that ATRA inhibited the malignancy of HCC cells by improving ferroptosis,and that inhibition of GSH synthesis contributed to ATRA-induced ferroptosis.

关键词： All-trans retinoic acid Ferroptosis Hepatocarcinoma Invasion Migration

NIR-II live imaging study on the degradation pattern of collagen in the mouse model

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Regenerative Biomaterials 2023年第1期10卷 271-279页

作者： Huizhu Li Xinxian Meng Huaixuan Sheng Sijia Feng Yuzhou Chen Dandan Sheng Liman Sai Yueming Wang Mo Chen Yan Wo Shaoqing Feng Hossein Baharvand Yanglai Gao Yunxia Li Jun Chen department of Sports Medicine Sports Medicine Institute of Fudan UniversityHuashan HospitalFudan UniversityShanghai 200040China department of Plastic and Reconstructive surgery School of MedicineShanghai Jiao Tong UniversityShanghai Ninth People’s HospitalShanghai 200011China department of Physics Shanghai Normal UniversityShanghai 200234China department of Anatomy and Physiology School of MedicineShanghai Jiao Tong UniversityShanghai 200025China department of stem cells and Developmental biology Cell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyACECRTehran ***Iran department of Developmental biology School of Basic Sciences and Advanced Technologies in BiologyUniversity of Science and CultureTehran ***Iran Hexi College ZhangyeGansu 73400China

The degradation of collagen in different body parts is a critical point for designing collagen-based biomedical ***,three kinds of collagens labeled by second near-infrared(NIR-II)quantum dots(QDs),including collagen with low crosslinking degree(LC),middle crosslinking degree(MC)and high crosslinking degree(HC),were injected into the subcutaneous tissue,muscle and joints of the mouse model,respectively,in order to investigate the in vivo degradation pattern of collagen by NIR-II live *** results of NIR-II imaging indicated that all tested collagens could be fully degraded after 35 days in the subcutaneous tissue,muscle and joints of the mouse ***,the average degradation rate of subcutaneous tissue(k=0.13)and muscle(k=0.23)was slower than that of the joints(shoulder:k=0.42,knee:k=0.55).Specifically,the degradation rate of HC(k=0.13)was slower than LC(k=0.30)in muscle,while HC showed the fastest degradation rate in the shoulder and knee *** summary,NIR-II imaging could precisely identify the in vivo degradation rate of ***,the degradation rate of collagen was more closely related to the implanted body parts rather than the crosslinking degree of collagen,which was slower in the subcutaneous tissue and muscle compared to the joints in the mouse model.

关键词： collagen degradation rate NIR-II live imaging in vivo crosslinking degree

New evidence for the epigenetic regulation of skeletal stem cells

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Science Bulletin 2024年第13期69卷 2004-2006页

作者： Liming Zhao Jun Xiao Charles K.F.Chan Institute for stem cell biology and Regenerative Medicine Stanford University School of MedicineStanford CA 94305USA department of surgery Division of Plastic and Reconstructive SurgeryStanford University School of MedicineStanford CA 94305USA department of Orthopaedic surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Skeletal stem cells(SSCs)are crucial for skeletal development and regeneration,yet the epigenetic regulation of these cells remains poorly *** studies have delineated the epigenetic landscape across the developmental trajectory of SSCs,highlighting the involvement of Ptip in regulating SSC *** skeletal system undergoes dynamic remodeling throughout *** stem cells(SSCs),residing within the skeleton and lineage-restricted,serve as a cellular reserve to foster development and maintain skeletal *** categorized within the broader mesenchymal stromal/stem cells(MSCs).

关键词： skeletal involvement maintain

PIP5k1β controls bone homeostasis through modulating both osteoclast and osteoblast differentiation

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Journal of Molecular cell biology 2020年第1期12卷 55-70页

作者： Xiaoying Zhao Penglei Cui Guoli Hu Chuandong Wang Lei Jiang Jingyu Zhao Jiake Xu Xiaoling Zhang department of Orthopedic surgery Xinhua HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200092China The Key laboratory of stem cell biology Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200025China School of Pathology and laboratory Medicine University of Western AustraliaPerthWestern Australia6009Australia Key laboratory of Tibetan Medicine Research Northwest Plateau Institute of BiologyChinese Academy of SciencesXining 810001China

PIP5k1βis crucial to the generation of phosphotidylinosotol(4,5)***5k1βparticipates in numerous cellular activities,such as B cell and platelet activation,cell phagocytosis and endocytosis,cell apoptosis,and cytoskeletal *** the present work,we aimed to examine the function of PIP5k1βin osteoclastogenesis and osteogenesis to provide promising strategies for osteoporosis prevention and *** discovered that PIP5k1β deletion in mice resulted in obvious bone loss and that PIP5k1β was highly expressed during both osteoclast and osteoblast *** of the gene was found to enhance the proliferation and migration of bone marrow-derived macrophage-like cells to promote osteoclast ***5k1β-/-osteoclasts exhibited normal cytoskeleton architecture but stronger resorption ***5kip deficiency also promoted activation of mitogen-activated kinase and Akt signaling,enhanced TRAF6 and c-Fos expression,facilitated the expression and nuclear translocation of NFATC1,and upregulated Grb2 expression,thereby accelerating osteoclast differentiation and ***,PIP5k1β enhanced osteoblast differentiation by upregulating master gene expression through triggering smad1/5/8 ***,PIP5k1βmodulates bone homeostasis and remodeling.

关键词： PIP5k1β osteoclast differentiation osteoblast differentiation proliferation migration NFATC1

A tunable, rapid, and precise drug control of protein expression by combining transcriptional and post-translational regulation systems

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Journal of Genetics and Genomics 2020年第11期47卷 705-712页

作者： Li Li Hongyi Meng Jianmin Zhang Yang Liu Qingjian Zou Yi Gao Huaqiang Yang Liangxue Lai department of Hepatobiliary surgery II Guangdong Provincial Research Center for Artificial Organ and Tissue EngineeringInstitute of Regenerative MedicineZhujiang HospitalSouthern Medical UniversityGuangzhou510282China CAS Key laboratory of Regenerative biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineSouth China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou510530China School of Biotechnology and Health Sciences Wuyi UniversityJiangmen529020China College of Animal Science South China Agricultural UniversityGuangzhou510642China

Rapid,precise,and tunable regulation of protein abundance would be significantly useful in a variety of biotechnologies and biomedical ***,we describe a system that allows tunable and rapid drug control of gene expression for either gene activation or inactivation in mammalian *** construct the system by coupling Tet-on 3 G and small molecule-assisted shutoff systems,which can respectively induce transcriptional activation and protein degradation in the presence of corresponding small *** dual-input drug inducer regulation system facilitates a bidirectional control of gene *** gene of interest can be precisely controlled by dual small molecules in a broad dynamic range of expression from overexpression to complete silence,allowing gene function study in a comprehensive expression *** results reveal that the bidirectional control system enables sensitive dosage-and time-dependent regulation for either turn-on or shutoff of gene *** also apply this system for inducible genome editing and gene activation mediated by clustered regularly interspaced short palindromic *** system provides an integrated platform for studying multiple biological processes by manipulating gene expression in a more flexible way.

关键词： Protein abundance Tet-on 3G SMASh Gene expression CRISPR Small molecule

Myogenesis controlled by a long non-coding RNA 1700113A16RIK and post-transcriptional regulation

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cell Regeneration 2022年第1期11卷 120-130页

作者： Xin Fu Sheng Li Minzhi Jia Bo Xu Lele Yang Ruimiao Ma Hong Cheng Wenjun Yang Ping Hu Spine Center Department of Pediatric OrthopedicsXin Hua Hospital Affiliated to Shanghai Jiao Tong UniversitySchool of MedicineShanghai 200092China State Key laboratory of cell biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China Guangzhou laboratory Guangzhou 510700GuangdongChina State Key laboratory of Molecular biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China Institute for stem cell and Regeneration Chinese Academy of SciencesBeijing 100101China

Long non-coding(lnc)RNA plays important roles in many cellular *** function of the vast majority of lncRNAs remains *** we identified that lncRNA-1700113A16RIK existed in skeletal muscle stem cells(MuSCs)and was significantly elevated during MuSC *** of 1700113A16RIK inhibits the differentiation of muscle stem *** contrast,overexpression of 1700113A16RIK promotes the differentiation of muscle stem *** study shows the muscle specific transcription factor Myogenin(MyoG)positively regulates the expression of 1700113A16RIK by binding to the promoter region of ***,1700113A16RIK may regulate the expression of myogenic genes by directly binding to 3’UTR of an important myogenic transcription factor MEF2D,which in turn promotes the translation of *** together,our results defined 1700113A16RIK as a positive regulator of MuSC differentiation and elucidated a mechanism as to how 1700113A16RIK regulated MuSC differentiation.

关键词： Long non-coding RNA(lncRNA) 1700113A16RIK Muscle stem cell(MuSC)differentiation Myocyte-specific enhancer binding factor 2(MEF2D)

Comparison of perioperative outcomes between laparoscopic and open partial splenectomy in children and adolescents

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World Journal of Gastrointestinal surgery 2021年第9期13卷 979-987页

作者： Mohamed Makansi Martin Hutter Till-Martin Theilen Henning C Fiegel Udo Rolle Stefan Gfroerer department of pediatric surgery and pediatric Urology University Hospital FrankfurtFrankfurt am Main 60590Germany department of pediatric surgery and pediatric Urology Helios Clinic Berlin-BuchBerlin 13125Germany

BACKGROUND In order to avoid consequences of total splenectomy,partial splenectomy(PS)is increasingly *** purpose of this study was to compare perioperative outcomes of laparoscopic PS(LPS)and open PS(OPS)in children and *** To compare perioperative outcomes of patients with LPS and *** After institutional review board approval,a total of 26 patients that underwent LPS or OPS between January 2008 and July 2018 were identified from the database of our tertiary referral *** total,10 patients had LPS,and 16 patients underwent *** loss was calculated by Mercuriali’s *** scores,analgesic requirements and complications were *** Wilcoxon rank sum test was used for *** compare categorical variables,Fisher’s exact test was *** LPS was performed in 10 patients;16 patients had ***(except for body mass index and duration of follow-up),indicating primary disease,preoperative spleen size and postoperative spleen volume,perioperative hematological parameters,postoperative pain scores,analgesic requirements,adverse events according to the Clavien-Dindo classification and the comprehensive complication index,median time from operation to initiation of feeds,median time from operation to full feeds,median time from operation to mobilization and median length of hospital stay did not differ between LPS and ***(range)operative time(min)was longer in LPS compared to the OPS group[185(135-298)vs 144(112-270),respectively;P=0.048].Calculated perioperative blood loss(mL of red blood cell count)was higher in the LPS group compared to OPS[87(-45-777)vs-37(-114-553),respectively;P=0.039].CONCLUSION This is the first study that compared outcomes of LPS and *** operative approaches had comparable perioperative *** appears to be a viable alternative to OPS.

关键词： Laparoscopic vs open Laparoscopy Partial splenectomy Perioperative outcome Children Adolescents