NIR-II live imaging study on the degradation pattern of collagen in the mouse model

作     者：Huizhu Li Xinxian Meng Huaixuan Sheng Sijia Feng Yuzhou Chen Dandan Sheng Liman Sai Yueming Wang Mo Chen Yan Wo Shaoqing Feng Hossein Baharvand Yanglai Gao Yunxia Li Jun Chen 

作者机构：Department of Sports MedicineSports Medicine Institute of Fudan UniversityHuashan HospitalFudan UniversityShanghai 200040China Department of Plastic and Reconstructive SurgerySchool of MedicineShanghai Jiao Tong UniversityShanghai Ninth People’s HospitalShanghai 200011China Department of PhysicsShanghai Normal UniversityShanghai 200234China Department of Anatomy and PhysiologySchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of Stem Cells and Developmental BiologyCell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyACECRTehran ***Iran Department of Developmental BiologySchool of Basic Sciences and Advanced Technologies in BiologyUniversity of Science and CultureTehran ***Iran Hexi CollegeZhangyeGansu 73400China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2023年第10卷第1期

页      面：271-279页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：supported by National Key R&D Program of China(2021YFA1201303) National Natural Science Foundation of China(82172511,81972121,81972129,82072521,82011530023 and 82111530200) Sanming Project of Medicine in Shenzhen(SZSM201612078) the Introduction Project of Clinical Medicine Expert Team for Suzhou(SZYJTD201714) Shanghai Talent Development Funding Scheme(2020080) Shanghai Sailing Program(21YF1404100 and 22YF1405200) Shanghai Committee of Science and Technology(22DZ2204900) Medical Engineering Joint Fund of Fudan University(YG2022-14) 

主　　题：collagen degradation rate NIR-II live imaging in vivo crosslinking degree 

摘      要：The degradation of collagen in different body parts is a critical point for designing collagen-based biomedical ***,three kinds of collagens labeled by second near-infrared(NIR-II)quantum dots(QDs),including collagen with low crosslinking degree(LC),middle crosslinking degree(MC)and high crosslinking degree(HC),were injected into the subcutaneous tissue,muscle and joints of the mouse model,respectively,in order to investigate the in vivo degradation pattern of collagen by NIR-II live *** results of NIR-II imaging indicated that all tested collagens could be fully degraded after 35 days in the subcutaneous tissue,muscle and joints of the mouse ***,the average degradation rate of subcutaneous tissue(k=0.13)and muscle(k=0.23)was slower than that of the joints(shoulder:k=0.42,knee:k=0.55).Specifically,the degradation rate of HC(k=0.13)was slower than LC(k=0.30)in muscle,while HC showed the fastest degradation rate in the shoulder and knee *** summary,NIR-II imaging could precisely identify the in vivo degradation rate of ***,the degradation rate of collagen was more closely related to the implanted body parts rather than the crosslinking degree of collagen,which was slower in the subcutaneous tissue and muscle compared to the joints in the mouse model.