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Psychological Impacts of COVID-19 Pandemic on the Brazilian Population: Occupational Analysis

World Journal of Neuroscience 2021年第2期11卷 145-160页

作者： Lucas Emmanuel Pedro de Paiva Teixeira Renato Leonardo de Freitas Alberto Abad Juliana Almeida da Silva Mayra Antonelli-Ponti Cláudia Helena Cerqueira Mármora Luis Antonio Monteiro Campos Scheila Paiva Sandra Bastos José Aparecido Da Silva Institute of Motricity Sciences Federal University of Alfenas (UNIFAL-MG) Alfenas Brazil Behavioural Neurosciences Institute (INeC) Ribeir&#227o Preto Brazil Biomedical Sciences Institute Federal University of Alfenas (UNIFAL) Alfenas Brazil Laboratory of Neurosciences of Pain & Emotions and Multi-User Centre of neuroelectrophysiology Department of Surgery and Anatomy Ribeir&#227o Preto Medical School of the University of S&#227o Paulo Ribeir&#227o Preto Brazil. Psychology Graduate Program Federal University of Juiz de Fora (UFJF) Juiz de Fora Brazil Laboratory of Studies and Research in Social Economy University of S&#227o Paulo at Ribeir&#227o Preto Ribeir&#227o Preto Brazil department of the Old Adult and Maternal-infant School of Physical Therapy Graduate Program in Psychology Federal University of Juiz de Fora (UFJF) Juiz de Fora Brazil Master’s Program in Psychology Catholic University of Petrópolis Petrópolis Brazil. Department of Psychology Pontifical Catholic University of Rio de Janeiro Rio de Janeiro Brazil Psychology Graduate Program Federal University of Juiz de Fora (UFJF) Juiz de Fora Brazil. Otorhinolaryngology Institute (ISBO) S&#227o Paulo Brazil Laboratory of Psychophysics Perception Psychometrics and Pain University of S&#227o Paulo at Ribeir&#227o Preto Ribeir&#227o Preto Brazil

Context and objectives :The unexpected pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) affected several activities and exposed workers, students and the large mass of unemployed people to an economic and psychological pressure that could lead to various problems, such as anxiety, fear, depression and insomnia, consequently causing impairment of quality of life. Method: A cross-sectional study was conducted using the COVID-19 Peri-Traumatic Distress Index (CPDI) and the Fear of COVID-19 Scale (FCV-19S) to measure peri-traumatic stress and fear, such as psychological reactions during the 2019 coronavirus disease (COVID-19) pandemic. An online spreadsheet was used as a data collection tool to send the questionnaire and scales to a sample of 1,844 participants. Data were analyzed in groups of health professionals, students and teachers, and an analysis was performed between unemployed and employed people in order to evaluate the impact of employment on the population’s mental health. Results: All groups presented from moderate to high CPDI and FCV-19S scores, and students and unemployed people had higher levels of stress and fear. Conclusion: COVID-19 pandemic had a significant impact on the Brazilian population, with students and the unemployed showing the highest rates of stress and fear.

关键词： Mental Health Fear Peri-Traumatic Stress COVID-19 Student Unemployed

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Nikethamide affects inspiratory neuron discharge in the nucleus retrofacialis medial region in brain slices from neonatal rats

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Neural Regeneration Research 2010年第4期5卷 287-290页

作者： Zhibin Qian Mingli Ji Zhonghai Wu department of Physiology Southern Medical University Guangzhou 510515 Guangdong Province China College of Basic Medical Science Xinxiang Medical University Xinxiang 453003 Henan Province China

BACKGROUND： Nikethamide, a respiratory center stimulant, is widely used in China. However, its effects on the central nervous system and medullary respiratory center remain poorly understood. OBJECTIVE： To investigate the influence of nikethamide on inspiratory neuron discharge in the medial region of the nucleus retrofacialis in neonatal rats, based on the observations addressing rhythmic respiratory discharge generated by the basic medullary respiratory center and various respiration neuron discharges in brain slices. DESIGN, TIME AND SETTING： A controlled, observational study utilizing in vitro neuroelectrophysiology was performed at the department of Physiology in Southern Medical University between September and December in 2007. MATERIALS： Nikethamide was purchased from Sigma, USA; BL-420E biological signal collection and manaclement system was provided by Chengdu TME Technology, ***： Isolated medulla-spinal cord preparations were collected from neonatal Sprague Dawley rats, aged 1-3 days. Tissues were divided to include the medial region of the nucleus retrofacialis, ventral respiratory, and dorsal respiratory groups. Subsequently, modified Kreb＇s solution and 5 μg/mL nikethamide-containing modified Kreb＇s solution were consecutively perfused into the medial region of the nucleus retrofacialis in neonatal rat brain slices. MAIN OUTCOME MEASURES： Hypoglossal nerve root respiratory-related rhythmic discharge activities and inspiratory neuron discharges were recorded with an adsorption electrode and microelectrode. RESULTS Nikethamide resulted in prolonged inspiratory neuron discharge time, shortened respiratory cycle and expiratory time. Nikethamide intervention resulted in enhanced integral amplitude of some inspiratory neurons with no changes in discharge frequency or increased discharge frequency in remaining inspiratory neurons with no changes in integral amplitude. CONCLUSION： Nikethamide excites inspiratory neurons in the basic rhythmic respirat

关键词： nikethamide medial region of nucleus retrofacialis brainstem slices inspiratory neurons

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Microelectrode Array-evaluation of Neurotoxic Effects of Magnesium as an Implantable Biomaterial

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Journal of Materials Science & Technology 2016年第1期32卷 89-96页

作者： Ting Huang ZhonghaiWang Lina Wei Mark Kindy Yufeng Zheng Tingfei Xi Bruce Z. Gao Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China department of Bioengineering Clemson University Clemson SC29634 USA departments of Neurosciences and Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston SC29466 USA Ralph H. Johnson VA Medical Center Charleston SC29403 USA department of Materials Science and Engineering College of Engineering Peking University Beijing 100871 China Shenzhen Key Laboratory of Human Tissue Regeneration and Repair Shenzhen Institute Peking University Shenzhen 518057 China

Magnesium （Mg）-based biomaterials have shown great potential in clinical applications. However, the cytotoxic effects of excessive Mg2. and the corrosion products from Mg-based biomaterials, particularly their effects on neurons, have been little studied. Although viability tests are most commonly used, a functional evaluation is critically needed. Here, both methyl thiazolyl tetrazolium （MTT） and lactate de- hydrogenase （LDH） assays were used to test the effect of Mg2. and Mg-extract solution on neuronal viability. Microelectrode arrays （MEAs）, which provide long-term, real-time recording of extracellular electro- physiological signals of in vitro neuronal networks, were used to test for toxic effects. The minimum effective concentrations （ECmin） of Mg2. from the MTr and LDH assays were 3 mmol/L and 100 mmol/L respec- tively, while the ECmin obtained from the MEA assay was 0.1 mmol/L MEA data revealed significant loss of neuronal network activity when the culture was exposed to 25% Mg-extract solution, a concentra- tion that did not affect neuronal viability. For evaluating the biocompatibility of Mg-based biomaterials with neurons, MEA electrophysiological testing is a more precise method than basic cell-viability testing.

关键词： Magnesium Microelectrode array neuroelectrophysiology Neuron viability Neuronal network

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Intraoperative neurophysiological monitoring in selective dorsal rhizotomy (SDR)

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Brain Science Advances 2020年第1期6卷 56-67页

作者： Wenbin Jiang Qijia Zhan Junlu Wang Rong Mei Bo Xiao department of Neurosurgery Shanghai Children’s HospitalShanghai Jiaotong UniversityShanghai 200062China

For decades,intraoperative neurophysiological monitoring(IONM)has been used to guide selective dorsal rhizotomy(SDR)for the treatment of spastic cerebral palsy(CP).Electromyography(EMG)interpretation methods,which are the core of IONM,have never been fully discussed and addressed,and their importance and necessity in SDR have been questioned for ***,outcomes of CP patients who have undergone IONM-guided SDR have been favorable,and surgery-related complications are extremely *** this paper,we review the history of evolving EMG interpretation methods as well as their neuroelectrophysiological basis.

关键词： spasticity selective dorsal rhizotomy(SDR) rootlet selection neuroelectrophysiology electromyography(EMG)interpretation

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靳三针疗法治疗卒中后认知障碍的临床研究

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Journal of Acupuncture and Tuina Science 2020年第1期18卷 40-46页

作者：姚睿巩尊科张凯维 Xuzhou Hospital Affiliated to Nanjing University of Chinese Medicine Jiangsu 221000China department of Rehabilitation Science of Xuzhou Central Hospital Jiangsu 221000China 不详

目的:观察靳三针治疗卒中后认知障碍的临床疗效,以及对神经电生理(事件相关电位)的影响.方法:选取卒中后认知障碍患者60例,按照随机数字表法分为治疗组和对照组,每组30例.对照组患者予常规治疗,治疗组患者在常规治疗基础上加靳三针疗法... 详细信息

目的:观察靳三针治疗卒中后认知障碍的临床疗效,以及对神经电生理(事件相关电位)的影响.方法:选取卒中后认知障碍患者60例,按照随机数字表法分为治疗组和对照组,每组30例.对照组患者予常规治疗,治疗组患者在常规治疗基础上加靳三针疗法,两组均连续治疗4周.比较治疗前后两组简易精神状态检查量表(MMSE)和蒙特利尔认知评估量表(MoCA)评分,以及P300波幅和潜伏期.结果:治疗前两组MMSE和MoCA评分、P300潜伏期和P300波幅差异均无统计学意义(均P>0.05).治疗4周后,两组MMSE和MoCA评分及P300波幅均较治疗前提高,P300潜伏期较治疗前缩短,组内及组间差异均有统计学意义(均P<0.05).结论:常规治疗基础上加靳三针疗法治疗卒中后认知障碍疗效可靠.其机制可能是通过调控患者的神经电生理改善患者的认知状态.

关键词：针刺疗法靳三针头针刺激区中风后遗症认知障碍精神病状态评定量表神经心理学量表事件相关电位,P300

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