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Differentiation of Wharton's jelly mesenchymal stem cells into neurons in alginate scaffold

Neural Regeneration Research 2015年第8期10卷 1312-1316页

作者： Seyed Mojtaba Hosseini Attiyeh Vasaghi Newsha Nakhlparvar Reza Roshanravan Tahereh Talaei-khozani Zahra Razi Student Research Committee Shiraz University of Medical Sciences Cell and molecular Medicine Student Research Group Medical School Shiraz University of Medical Sciences Stem Cell Laboratory Department of Anatomy Shiraz University of Medical Sciences Colorectal Research Center Department of Surgery Shiraz University of Medical Science Tissue Engineering Laboratory Department of Tissue Engineering School of Advanced Medical Science and Technology Shiraz University of Medical Sciences Laboratory for Stem Cell Research Department of Anatomy Medical School Shiraz University of Medical Sciences department of Medical Physics Shiraz University of Medical Sciences

Alginate scaffold has been considered as an appropriate biomaterial for promoting the differentiation of embryonic stem cells toward neuronal cell lineage. We hypothesized that alginate scaffold is suitable for culturing Wharton’s jelly mesenchymal stem cells（WJMSCs） and can promote the differentiation of WJMSCs into neuron-like cells. In this study, we cultured WJMSCs in a three-dimensional scaffold fabricated by 0.25% alginate and 50 m M Ca Cl2 in the presence of neurogenic medium containing 10 μM retinoic acid and 20 ng/m L basic fibroblast growth factor. These cells were also cultured in conventional two-dimensional culture condition in the presence of neurogenic medium as controls. After 10 days, immunofluorescence staining was performed for detecting β-tubulin（marker for WJMSCs-differentiated neuron） and CD271（motor neuron marker）. β-Tubulin and CD271 expression levels were significantly greater in the WJMSCs cultured in the three-dimensional alginate scaffold than in the conventional two-dimensional culture condition. These findings suggest that three-dimensional alginate scaffold cell culture system can induce neuronal differentiation of WJMSCs effectively.

关键词： nerve regeneration Wharton’s jelly mesenchymal stem cells mesenchymal stem cells neurons motor neurons alginate 3D scaffold neural regeneration

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Subchronic toxicological effects of aquatic nitrobenzene on Medaka and Chinese rare minnow

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Science China Chemistry 2007年第5期50卷 707-717页

作者： ZHOU QunFang1, FU JianJie1,2, MENG HaiZhen1,2, ZHU XueYan1,2, JIANG GuiBin1, ZHANG JianBin1, LIU JieMin2 & SHI GuoQing2 1 State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental sciences, Chinese Academy of sciences, Beijing 100085, China 2 department of Chemistry, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China State Key Laboratory of Environmental Chemistry and Ecotoxicology ResearchCenter for Eco-environmental SciencesChinese Academy of SciencesBeijing 100085China department ofChemistry School of Applied ScienceUniversity of Science and Technology B

Based on the simulative exposure experiments of nitrobenzene to two small experimental fishes, Medaka (Oryzias latipes) and Chinese Rare Minnow (Gobiocypris rarus), it was found that nitrobenzene could decrease in a linear way in the static aquatic system. Both fishes could accumulate dose-related levels of nitrobenzene quickly and eliminate the compound rapidly when they were transferred to clean water. The Chinese rare minnow showed more sensitivity to the acute toxicity exposure of nitrobenzene than Medaka. Typical molecular biomarkers in oxidative defense system including superoxide dismu- tase (SOD), catalase (CAT) showed the chemical induced alterations. The changes of acetylcholi- nesterase (AChE) activities indicated that nitrobenzene might affect the normal neural function. Nitro- benzene exposure could also lead to obvious damaging effects on the target organs, such as gill and liver by hitopathological studies.

关键词： nitrobenzene, Medaka, Chinese rare minnow, bioaccumulation, elimination, molecular biomarkers, histopathological effects

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Metallothionein 1E Alleviates Cadmium-induced Renal Cytotoxicity through Promoting Mitochondrial Functional Recovery

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Biomedical and Environmental sciences 2024年第1期37卷 117-121页

作者： WEN Si Hui LI Lu Bei HUANG Hui Dan XIE Ying LUO Lei Key Laboratory of molecular Epidemiology of Hunan Province School of MedicineHunan Normal UniversityChangsha 410081HunanChina department of Occupational health Changsha Centre for Disease Control and PreventionChangsha 410004HunanChina The Key Laboratory of Model Animals and Stem Cell Biology in Hunan Province Hunan Normal University School of MedicineChangsha 410013HunanChina

The pervasive utilization of industrial substances has escalated human exposure to cadmium(Cd),a metal associated with long-term negative health outcomes such as renal dysfunction,neurological disorders,and various cancers^([1]).Once ingested by humans,Cd interacts with cysteine-rich metallothioneins(MTs)which have metal-binding and antioxidant properties and is subsequently transported to the kidney^([2]).

关键词： Renal kidney cadmium

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LipR functions as an intracellular pH regulator in Bacillus thuringiensis under glucose conditions

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mLife 2023年第1期2卷 58-72页

作者： Xia Cai Jiaxin Qin Xuelian Li Taoxiong Yuan Bing Yan Jun Cai department of Microbiology College of Life SciencesNankai UniversityTianjinChina School of Life Science and Engineering Lanzhou University of TechnologyLanzhouChina Key Laboratory of molecular Microbiology and Technology Ministry of EducationTianjinChina Tianjin Key Laboratory of Microbial Functional Genomics TianjinChina

Intracellular pH critically affects various biological processes,and an appropriate cytoplasmic pH is essential for ensuring bacterial *** is the preferred carbon source for most heterotrophs;however,excess glucose often causes the accumulation of acidic metabolites,lowering the intracellular pH and inhibiting bacterial *** thuringiensis can effectively cope with glucose-induced stress;unfortunately,little is known about the regulators involved in this ***,we document that the target of the dual-function sRNA YhfH,the lipR gene,encodes a LacI-family transcription factor LipR as an intracellular pH regulator when *** BMB171 is suddenly exposed to *** glucose conditions,lipR deletion leads to early growth arrest by causing a rapid decrease in intracellular pH(~5.4).Then,the direct targets and a binding motif(GAWAWCRWTWTCAT)of LipR were identified based on the electrophoretic mobility shift assay,the DNase-I footprinting assay,and RNA sequencing,and the gapN gene encoding a key enzyme in glycolysis was directly inhibited by ***,Ni^(2+)is considered a possible effector for *** addition to YhfH,the lipR expression was coregulated by itself,CcpA,and *** study reveals that LipR plays a balancing role between glucose metabolism and intracellular pH in *** subjected to glucose stress.

关键词： glucose intracellular pH LacI‐type transcription factor LipR

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The influence of bacteria on multitrophic interactions among plants, psyllids, and pathogen

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Insect Science 2017年第6期24卷 961-974页

作者： Cecilia Tamborindeguy Ordom Brian Huot Freddy Ibanez Julien Levy department of Entomology Texas A&M University College Station Texas USA department of Horticultural sciences Texas A&M University College Station Texas USA

The recent emergence ofseveralplant diseases caused by psyllid-borne bacterial pathogens worldwide （Candidatus Liberibaeter spp.） has created renewed interest on the interaction between psyllids and bacteria. In spite of these efforts to understand psyllid association with bacteria, many aspects of their interactions remain poorly understood. As more organisms are studied, subtleties on the molecular interactions as well as on the effects of the bacteria on the psyllid host are being uncovered. Additionally, psyllid-borne bacterial phytopathogens can also affect the host plant, which in turn can impact psyllid physiology and behavior. Here, we review the current literature on different aspects of the influence of bacteria on multitrophic interactions among plants, psyllids, and pathogens. We then highlight gaps that need to be addressed to advance this field, which can have significant implications for controlling these newly emergent and other plant diseases.

关键词： bacteria interaction Liberibacter microbiome phytoplasma psyllid

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Warburg Effects in Cancer and Normal Proliferating Cells:Two Tales of the Same Name

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Genomics, Proteomics & Bioinformatics 2019年第3期17卷 273-286页

作者： Huiyan Sun Liang Chen Sha Cao Yanchun Liang Ying Xu The China-Japan Union Hospital Jilin UniversityChangchun 130033China MOE Key Laboratory of Symbolic Computation and Knowledge Engineering College of Computer Science and TechnologyJilin UniversityChangchun 130012China Computational Systems Biology Lab Department of Biochemistry and Molecular Biology and Institute of BioinformaticsUniversity of GeorgiaAthensGA 30602USA Faculty of health sciences University of MacaoTaipaMacao SAR 999078China department of Biostatistics School of MedicineIndiana UniversityIndianapolisIN 46202USA Zhuhai Laboratory of MOE Key Laboratory of Symbolic Computation and Knowledge Engineering Zhuhai College of Jilin UniversityZhuhai 519041China

It has been observed that both cancer tissue cells and normal proliferating cells(NPCs)have the Warburg *** goal here is to demonstrate that they do this for different *** accomplish this,we have analyzed the transcriptomic data of over 7000 cancer and control tissues of 14 cancer types in TCGA and data of five NPC types in *** analyses reveal that NPCs accumulate large quantities of ATPs produced by the respiration process before starting the Warburg effect,to raise the intracellular pH from 6.8 to 7.2 and to prepare for cell division *** cell cycle starts,the cells start to rely on glycolysis for ATP generation followed by ATP hydrolysis and lactic acid release,to maintain the elevated intracellular pH as needed by cell division since together the three processes are pH *** cells go back to the normal respirationbased ATP production once the cell division phase *** comparison,cancer cells have reached their intracellular pH at 7.4 from top down as multiple acid-loading transporters are up-regulated and most acid-extruding ones except for lactic acid exporters are *** cells use continuous glycolysis for ATP production as way to acidify the intracellular space since the lactic acid secretion is decoupled from glycolysis-based ATP generation and is pH balanced by increased expressions of acid-loading ***-expression analyses suggest that lactic acid secretion is regulated by external,non-pH related ***,our data strongly suggest that the two cell types have the Warburg effect for very different reasons.

关键词： Cancer Warburg effect Fenton reaction Cell proliferation pH homeostasis

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Spontaneous xenogeneic GvHD in Wilms'tumor Patient-Derived xenograft models and potential solutions

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Animal Models and Experimental Medicine 2022年第4期5卷 389-396页

作者： Seyed Mostafa Monzavi Ahad Muhammadnejad Maryam Behfar Amir Arsalan Khorsand Samad Muhammadnejad Abdol-Mohammad Kajbafzadeh department of Applied Cell sciences School of Advanced Technologies in MedicineTehran University of Medical SciencesTehranIran Pediatric Urology and Regenerative Medicine Research Center Tehran University of Medical SciencesTehranIran Gene Therapy Research Center Digestive Diseases Research InstituteTehran University of Medical SciencesTehranIran Cancer Biology Research Center Cancer Institute of IranTehran University of Medical SciencesTehranIran PDX Platform Biomarker Evaluation and Supervision Team for Personalized MedicineMolecular Tumor BoardCancer Institute of IranTehran University of Medical SciencesTehranIran Pediatric Cell and Gene Therapy Research Center Tehran University of Medical SciencesTehranIran

Severely immunocompromised ***-PrkdcIl2rg(NOG)mice are among the ideal animal recipients for generation of human cancer *** of human solid tumors having abundant tumor-i nfiltrating lymphocytes(TILs)can induce xenogeneic graft-versus-host disease(xGvHD)following engraftment and expansion of the TILs inside the animal ***’tumor(WT)has not been recognized as a lymphocyte-predominant ***,3 consecutive generations of NOG mice bearing WT patient-derived xenografts(PDX)xenotransplanted from a single donor showed different degrees of inflammatory symptoms after transplantation before any therapeutic *** the initial generation,dermatitis,auto-amputation of digits,weight loss,lymphadenopathy,hepatitis,and interstitial pneumonitis were *** antibiotic treatment,no response was noticed,and thus the animals were prematurely euthanized(day 47 posttransplantation).Laboratory and histopathologic evaluations revealed lymphoid infiltrates positively immunostained with anti-human CD3 and CD8 antibodies in the xenografts and primary tumor,whereas no microbial infection or lymphoproliferative disorder was *** of the next generation that lived longer(91 days)developed sclerotic skin changes and more severe *** symptoms were milder in the last *** xenografts of the last 2 generations also contained TILs,and lacked lymphoproliferative *** systemic immunoinflammatory syndrome in the absence of microbial infection and posttransplant lymphoproliferative disorder was suggestive of *** there are few reports of xGvHD in severely immunodeficient mice xenotransplanted from lymphodominant tumor xenografts,this report for the first time documented serial xGvHD in consecutive passages of WT PDX-bearing models and discussed potential solutions to prevent such an undesired complication.

关键词： graft-versus-host disease patient-derived xenograft models tumor-infiltrating lymphocytes Wilms’tumor

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Thermal Transport in Methane Hydrate by molecular Dynamics and Phonon Inelastic Scattering

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Chinese Physics Letters 2015年第10期32卷 72-75页

作者：王照亮苑昆鹏唐大伟 Energy and Power department China University of Petroleum Qingdao 266580 Institute of Engineering Thermophysics Chinese Academy of Sciences Beijing 100190

The heat conduction and thermal conductivity for methane hydrate are simulated from equilibrium molecular dynamics. The thermal conductivity and temperature dependence trend agree well with the experimental results. The nonmonotonic temperature dependence is attributed to the phonon inelastic scattering at higher temperature and to the confinement of the optic phonon modes and low frequency phonons at low temperature. The thermal conductivity scales proportionally with the van der Waals interaction strength, The conversion of a crystal-like nature into an amorphous one oecurs at higher strength. Both the temperature dependence and interaction strength dependence are explained by phonon inelastic scattering.

关键词： Thermal Transport in Methane Hydrate by molecular Dynamics and Phonon Inelastic Scattering THz

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Enhancing metformin efficacy with cholecalciferol and taurine in diabetes therapy:Potential and limitations

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World Journal of Diabetes 2025年第1期16卷 22-26页

作者： Gehan El-Akabawy Nabil Eid department of Basic Medical sciences College of MedicineAjman UniversityAjman 346United Arab Emirates department of Anatomy Division of Human BiologySchool of MedicineIMU UniversityKuala Lumpur 57000Malaysia

Diabetes mellitus,particularly type 2 diabetes mellitus(T2DM),poses a significant global health *** management strategies primarily focus on glycemic control;however,there is a growing need for comprehensive approaches addressing the complex pathophysiology of diabetes *** recent study by Attia et al explores the potential of a novel therapy combining metformin with cholecalciferol(vitamin D3)and taurine to mitigate T2DM-related complications in a rat *** findings indicate that this treatment combination improves glycemic control and reduces oxidative stress,inflammation,and lipid ***,the study is limited by a lack of safety profile data and in-depth molecular mechanism *** editorial critically highlights the study's strengths and weaknesses,compares it against other combination therapy research in T2DM,and underscores the need to explore further the mechanisms underpinning the observed therapeutic effects and investigate the safety profile of this novel approach.

关键词： Diabetes Metformin Cholecalciferol Taurine Hyperglycemia Oxidative stress Triglycerides

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Biological evaluation of antibody-maytansinoid conjugates as a strategy of RON targeted drug delivery for treatment of non-small cell lung cancer

Biological evaluation of antibody-maytansinoid conjugates as...

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浙江省免疫学会第十次学术大会

作者： Liang Feng Hang-Ping Yao Yong-Qing Zhou Jianwei Zhou Ruiwen Zhang Ming-Hai Wang State Key Laboratory for Diagnosis & Treatment of Infectious Diseases and Collaborative Innovation Center for Diagnosis & Treatment of Infectious Diseases First Hospital of Zhejiang University School of Medicine department of Biomedical sciences Texas Tech University Health Sciences Center School of Pharmacy1406 Coulter Street department of Neurosurgery First Hospital of Zhejiang University School of Medicine department of molecular Cell Biology & Toxicology Nanjing Medical University School of Public Health department of Pharmaceutical sciences Texas Tech University Health Sciences Center School of Pharmacy

Background:Aberrant expression of the RON receptor tyrosine kinase,a member of the MET proto-oncogene family,in breast cancer and non-small cell lung cancer(NSCLC) has therapeutic *** we evaluated the efficacy of a novel anti-RON antibody-drug maytansinoid conjugate Zt/g4-DM1 for treatment of breast and NSCLC xenograft tumors in mouse models and explored a treatment strategy by combination of Zt/g4-DM1 with chemotherapeutics to achieve the maximal therapeutic ***:Mouse monoclonal antibody Zt/g4(IgG1 a/κ) specific to human RON was conjugated to DM1 via thioether linkage to form Zt/g4-DM1 with a drug-antibody ratio of 4:*** breast cancer and NSCLC cell lines,expressing different levels of RON,were used as the *** was used to determine Zt/g4-induced RON *** cytometric analysis and cell viability assay were used to determine the effect of Zt-g4-DM1 on cell cycle and *** xenograft NSCLC models were used in vivo to determine the therapeutic efficacy of Zt/g4-DM1 alone or in combination with ***:In vitro,Zt/g4 treatment of breast cancer and NSCLC cells rapidly induced cell surface RON internalization,which results in intracellular delivery of DM1 sufficient to arrest cell cycle at G2/M phase,reduce cell viability,and cause massive cell *** mouse tumor xenograft models,Zt/g4-DM1 at 20 mg/kg in a Q12 × 2 regimen effectively blocked breast cancer and NSCLC cell-mediated tumor *** than 95%inhibition of tumor growth among three tumor xenograft models tested was achieved according to the measured tumor *** minimal dose to balance the tumor growth and inhibition(tumoristatic concentration) was estabhshed at 2.02 mg/kg for H2228,1.94 mg/kg for H358 cell,and 6.25 mg/kg for T-47 D cell-mediated xenograft ***:Zt/g4 is highly effective in RON-directed drug delivery for targeted inhibition of NSCLC cell-derived tumor growth in mouse xenograft ***

关键词： Antibody-drug conjugate Breast cancer Lung cancer Receptor tyrosine kinase Therapeutic efficacy Combination therapy

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