检索结果-南通市图书馆

CRISPR-mediated mutagenesis of the odorant receptor co-receptor(Orco)gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis

在线全文

学校读者我要写书评

暂无评论

insect Science 2022年第5期29卷 1275-1286页

作者： Li Xu Hong-Bo Jiang Kai-Yue Tang Ying Yan Marc F.Schetelig Jin-Jun Wang Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control Academy of Agricultural SciencesSouthwest UniversityChongqingChina Department of Insect Biotechnology in Plant Protection Justus-Liebig-University GiessenInstitute for Insect BiotechnologyGiessenGermany

Olfaction plays an essential role in insect behavior such as host location,foraging,mating,and oviposition.The odorant receptor co-receptor(Orco)is an obligatory odorant receptor and indispensable in odor perception.Here,we characterized the Orco gene from the oriental fruit fly,Bactrocera dorsalis(Hendel),a notorious agriculture pest.The olfactory deficiency mutants were generated by editing the BdorOrco gene using the CRISPR/Cas9 system.Electroantennograms(EAG)and olfactory preference assays confirmed that BdorOrco^(−/−)mutant flies had reduced perception of methyl eugenol,β-caryophyllene,and ethyl acetate.Oviposition bioassays showed that the eggs laid by BdorOrco^(−/−)females mediated by benzothiazole and 1-octen-3-ol were significantly decreased.In addition,BdorOrco^(−/−)mutant flies took a significantly longer time to locate the food source compared with wild type(WT)flies.Altogether,our data indicated that Orco is essential for multiple physiological processes in B.dorsalis,and it expands our understanding of the function of insect Orco.

关键词： CRISPR/Cas9 foraging olfaction oriental fruit fly oviposition

Chloroplast immunity: A cornerstone of plant defense

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular plant 2024年第5期17卷 686-688页

作者： Jie Liu Pan Gong Ruobin Lu Rosa Lozano-Duran Xueping Zhou Fangfang Li State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhouZhejiang 310058China department of plant Biochemistry Centre for Plant Molecular Biology(ZMBP)Eberhard Karls UniversityTubingen 72076Germany

Increasing evidence supports the notion that pattern-recognition-receptor-mediated immunity goes beyond plasma membrane-to-nucleus signaling.With the findings of the pathways linking plasma membrane and chloroplasts and the functions of stromules and perinuclear chloroplast clustering(PCC)in plant defense,chloroplast immunity has emerged as a cornerstone of plant defense and a target of plant pathogens.

关键词： plant immunity corners

抗虫抗草甘膦转基因玉米分子的特征及功能评价（英文）

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2018年第8期19卷 610-619页

作者： Miao-miao LIU Xiao-jing ZHANG Yan GAO Zhi-cheng SHEN Chao-yang LIN Institute of insect Science Department of Plant Protection Zhejiang University

目的:研究抗虫抗草甘膦玉米转化体ZD12-6外源基因的分子特征,对ZD12-6的抗虫和抗草甘膦性状进行综合评价,为ZD12-6产业化提供基础信息。创新点:抗虫抗草甘膦转基因玉米ZD12-6是聚合了双抗虫基因和抗草甘膦基因的转化体,多基因聚合有利... 详细信息

目的:研究抗虫抗草甘膦玉米转化体ZD12-6外源基因的分子特征,对ZD12-6的抗虫和抗草甘膦性状进行综合评价,为ZD12-6产业化提供基础信息。创新点:抗虫抗草甘膦转基因玉米ZD12-6是聚合了双抗虫基因和抗草甘膦基因的转化体,多基因聚合有利于后期品种转育和多性状叠加。对该转化体的分子特征信息分析和功能评价是评判其产业化价值的重要依据。方法:利用DNA印迹法(Southern blot)研究外源基因插入拷贝数;利用高效热不对称交错聚合酶链反应(hiTAIL-PCR)方法对外源基因的插入位点进行定位;通过蛋白质印迹法(Western blot)对外源蛋白进行定性分析;利用酶联免疫吸附测定(ELISA)分析外源蛋白表达量;通过生物测定对抗虫和草甘膦的抗性水平进行评估。结论:抗虫抗草甘膦玉米转化体ZD12-6中外源基因单拷贝插入,插入位点位于玉米1号染色体。ZD12-6对玉米上的主要害虫具有良好抗性,同时对草甘膦具有较强耐受性,具有产业化推广潜力。

关键词：转基因玉米苏云金芽孢杆菌抗虫抗草甘膦

SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants

在线全文

学校读者我要写书评

暂无评论

Molecular plant 2023年第3期16卷 632-642页

作者： Linhao Ge Buwei Cao Rui Qiao Hongguang Cui Shaofang Li Hongying Shan Pan Gong Mingzhen Zhang Hao Li Aiming Wang Xueping Zhou Fangfang Li State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBejingChina Key Laboratory of Green Prevention and Control of Tropical plant Diseases and Pests Ministry of Education and College of Plant ProtectionHainan UniversityHaikouHainanChina London Research and Development Centre Agriculture and Agri-Food CanadaLondonONCanada department of Biology Western UniversityLondonONCanada state Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhouZhejiangChina

RNA quality control nonsense-mediated decay is involved in viral restriction in both plants and animals.However,it is not known whether two other RNA quality control pathways,nonstop decay and no-go decay,are capable of restricting viruses in plants.Here,we show that the evolutionarily conserved Pelota–Hbs1 complex negatively regulates infection of plant viruses in the family Potyviridae(termed potyvirids),the largest group of plant RNA viruses that accounts for more than half of the viral crop damage worldwide.Pelota enables the recognition of the functional G1-2A6-7 motif in the P3 cistron,which is conserved in almost all potyvirids.This allows Pelota to target the virus and act as a viral restriction factor.Furthermore,Pelota interacts with the SUMO E2-conjugating enzyme SCE1 and is SUMOylated in planta.Blocking Pelota SUMOylation disrupts the ability to recruit Hbs1 and inhibits viral RNA degradation.These findings reveal the functional importance of Pelota SUMOylation during the infection of potyvirids in plants.

关键词： SUMOylation Pelota-Hbs1 G1-2A6-7 motif Potyviruses

Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular plant 2021年第2期14卷 253-266页

作者： Jiyang Wang Ruyi Wang Hong Fang Chongyang Zhang Fan Zhang Zeyun Hao Xiaoman You Xuetao Shi Chan Ho Park Kangyu Hua Feng He Maria Bellizzi Kieu Thi Xuan Vo Jong-Seong Jeon Yuese Ning Guo-Liang Wang State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China department of plant Pathology Ohio State UniversityColumbusOH 43210USA Graduate School of biotechnology and Crop Biotech Institute Kyung Hee UniversityYongin 17104Korea

Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant immunity.However,how NLRs are regulated and activate defense signaling is not fully understood.The rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10(APIP10)negatively regulates Piz-t accumulation.In this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR plant ONEZINC FINGER 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome pathway.OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta.The osvoz1 and osvoz2 single mutants display modest but opposite M.oryzae resistance in the non-Piz-t background.However,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to M.oryzae in the non-Piz-t background.Both OsVOZ1 and OsVOZ2 interact with Piz-t.Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to M.oryzae containing AvrPiz-t.Taken together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity.

关键词： rice E3 ligase VOZ transcription factor NLR protein fungal pathogen Magnaporthe oryzae cell death

Integrated pest management programme for cereal blast fungus Magnaporthe oryzae

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第12期21卷 3420-3433页

作者： ZHANG Hai-feng Tofazzal ISLAM LIU Wen-de department of plant Pathology College of Plant ProtectionNanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests of Ministry of EducationNanjing 210095P.R.China Institute of biotechnology and Genetic Engineering Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur 1706Bangladesh State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Magnaporthe oryzae,the causal agent of blast diseases,is a destructive filamentous fungus that infects many plants including most economically important food crops,rice,wheat,pearl millet and finger millet.Magnaporthe oryzae has numerous pathotypes because of its high host-specificity in the field.The Oryza pathotype(MoO)of M.oryzae is the most devastating pathogen of rice,causing 10–30%yield loss in the world.On the other hand,the Triticum pathotype(MoT)causes blast disease in wheat,which is now a serious threat to wheat production in some South American countries,Bangladesh and Zambia.Because of low fungicide efficacy against the blast diseases and lack of availability of resistant varieties,control of rice and wheat blast diseases is difficult.Therefore,an integrated management programme should be adopted to control these two diseases in the field.Here,we introduced and summarized the classification,geographical distribution,host range,disease symptoms,biology and ecology,economic impact,and integrated pest management(IPM)programme of both rice and wheat blast diseases.

关键词： rice blast wheat blast Magnaporthe oryzae integrated pest management

Monoclonal Antibody-Based Serological Detection of Rice Stripe Mosaic Virus Infection in Rice plants or Leafhoppers

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2020年第2期35卷 227-234页

作者： Liqian Guo Jiayu Wu Rui Chen Jian Hong Xueping Zhou Jianxiang Wu State Key Laboratory of Rice Biology Institute of Biotechnology.Zhejiang UniversityHangzhou 310058China department of Applied Biological Science College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China

Rice stripe mosaic virus(RSMV) is a rhabdovirus recently found in southern part of China and can cause severe reduction in rice production. To establish serological methods for RSMV epidemiological studies and to establish a control strategy for this virus, we first purified RSMV virions from infected rice plants and then used them as an immunogen to produce four RSMV-specific monoclonal antibodies(MAbs)(i.e.,1D4, 4A8, 8E4 and 11F11). With these MAbs, we have developed a highly specific and sensitive antigen-coated plate enzyme-linked immunosorbent assay(ACP-ELISA), a Dot-ELISA and a Tissue print-ELISA for rapid detections of RSMV infection in rice plants or in leafhoppers. Our results showed that RSMV can be readily detected in RSMV-infected rice plant tissue crude extracts diluted at 1:20,971,520(w/v, g/m L)through ACP-ELISA or diluted at 1:327,680(w/v, g/m L) through Dot-ELISA. Both ACP-ELISA and Dot-ELISA can also be used to detect RSMV infection in individual RSMV viruliferous leafhopper(Recilia dorsalis) homogenate diluted at 1:307,200 and 1:163,840(individual leafhopper/l L), respectively. Detection of RSMV infection in field-collected rice samples or in RSMV viruliferous leafhoppers indicated that the three serological methods can produce same results with that produced by RT-PCR(19 of the 33 rice samples and 5 of the 16 leafhoppers were RSMV-positive). We consider that the four MAbs produced in this study are very specific and sensitive, and the three new serological methods are very useful for detections of RSMV infection in rice plants or in leafhoppers and the establishment of the disease control strategies.

关键词： Rice stripe mosaic virus(RSMV) Serological test(ACP-ELISA) Dot-ELISA Tissue print-ELISA

Co-evolved plant and blast fungus ascorbate oxidases orchestrate the redox state of host apoplast to modulate rice immunity

在线全文

学校读者我要写书评

暂无评论

Molecular plant 2022年第8期15卷 1347-1366页

作者： Jiexiong Hu Muxing Liu Ao Zhang Ying Dai Weizhong Chen Fang Chen Wenya Wang Danyu Shen Mary Jeanie Telebanco-Yanoria Bin Ren Haifeng Zhang Huanbin Zhou Bo Zhou Ping Wang Zhengguang Zhang department of plant Pathology College of Plant ProtectionNanjing Agricultural Universityand Key Laboratory of Integrated Management of Crop Diseases and PestsMinistryof EducationNanjingChina The Key Laboratory of plant Immunity Nanjing Agricultural UniversityNanjingChina Genetics and biotechnology Division International Rice Research InstituteLos BanosLaguna 4031Philippines State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China department of Microbiology Immunologyand ParasitologyLouisiana State University Health Sciences CenterNew OrleansLAUSA

Apoplastic ascorbate oxidases(AOs)play a critical role in reactive oxygen species(RoS)-mediated innate host immunity by regulating the apoplast redox state.To date,little is known about how apoplastic effectors of the riceblast fungus Magnaportheoryzaemodulate the apoplast redox state of rice to subvert plant immunity.In this study,we demonstrated that M.oryzae MoAo1 is an Ao that plays a role in virulence by modulating the apoplast redox status of rice cells.We showed that MoAo1 inhibits the activity of rice OsAO3and OsAO4,which also regulate the apoplast redox status and plant immunity.In addition,we found that MoAo1,OsAO3,andOsAO4 allexhibit polymorphic variations whosevaried interactions orchestrate pathogen virulence and rice immunity.Taken together,our results reveal a critical role for extracellular redox enzymes during rice blast infection and shed light on the importance of the apoplast redox state anditsregulation inplant-pathogeninteractions.

关键词： apoplast redox state RoS ascorbate oxidase polymorphism rice blast host immunity

Achieving broad-spectrum resistance against rice bacterial blight through targeted promoter editing and pathogen population monitoring

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

aBIOTECH 2020年第2期1卷 119-122页

作者： Jiyang Wang Yuese Ning Irene N.Gentzel Guo-Liang Wang State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China department of plant Pathology The Ohio State UniversityColumbusOH 43210USA

plant diseases severely reduce crop yields and threaten global food security.Broad-spectrum resistance(BSR)is a desirable trait because it confers resistance against more than one pathogen species or the majority of races/strains of the same pathogen.To control plant diseases,breeders have selected BSR to reduce disease occurrence and prolong the life-span of newly released cultivars in the last several decades(Mundt,Phytopathology 108(7):792–802,2018).Although effective,breeding of BSR cultivars in crop plants is still time-consuming and technically challenging.Recently,new gene-editing technologies such as CRISPR/Cas9 have dramatically accelerated the process of plant breeding and provided an approach for rapidly creating new varieties with BSR and other beneficial traits(Borrelli et al.,Front plant Sci 9:1245,2018).In addition,close surveillance of pathogen populations in the field can provide useful information for the deployment of appropriate resistance genes in the target regions.In this mini-review,we focus on the significance and application of the exciting results from two recent companion papers published in Nature biotechnology that provide new strategies to develop crop plants with BSR against pathogens through targeted promoter editing of susceptibility genes in plants as well as pathogen population monitoring.

关键词： CRISPR/Cas9 Broad-spectrum resistance Rice bacterial blight Stacking editing Pathogen population monitoring