SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants
作者机构：State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBejingChina Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and PestsMinistry of Education and College of Plant ProtectionHainan UniversityHaikouHainanChina London Research and Development CentreAgriculture and Agri-Food CanadaLondonONCanada Department of BiologyWestern UniversityLondonONCanada state Key Laboratory of Rice BiologyInstitute of BiotechnologyZhejiang UniversityHangzhouZhejiangChina
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2023年第16卷第3期
页 面：632-642页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：funded by the National Key Research and Development Program of China(2021YFD1400400) National Natural Science Foundation of China(31972244)to F.L.
主 题：SUMOylation Pelota-Hbs1 G1-2A6-7 motif Potyviruses
摘 要：RNA quality control nonsense-mediated decay is involved in viral restriction in both plants and animals.However,it is not known whether two other RNA quality control pathways,nonstop decay and no-go decay,are capable of restricting viruses in plants.Here,we show that the evolutionarily conserved Pelota–Hbs1 complex negatively regulates infection of plant viruses in the family Potyviridae(termed potyvirids),the largest group of plant RNA viruses that accounts for more than half of the viral crop damage worldwide.Pelota enables the recognition of the functional G1-2A6-7 motif in the P3 cistron,which is conserved in almost all potyvirids.This allows Pelota to target the virus and act as a viral restriction factor.Furthermore,Pelota interacts with the SUMO E2-conjugating enzyme SCE1 and is SUMOylated in planta.Blocking Pelota SUMOylation disrupts the ability to recruit Hbs1 and inhibits viral RNA degradation.These findings reveal the functional importance of Pelota SUMOylation during the infection of potyvirids in plants.
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