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SARS-CoV-2 Spike protein promotes vWF secretion and thrombosis via endothelial cytoskeleton-associated protein 4(CKAP4)

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Signal Transduction and Targeted Therapy 2022年第10期7卷 3655-3657页

作者： Kan Li Liu Yao Jin Wang Hao Song Yan-hong Zhang Xue Bai Kai Zhang Dong-ming Zhou Ding Ai Yi Zhu The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics Department of Physiology and PathophysiologyTianjin Medical UniversityTianjinChina department of Biochemistry and Molecular biology Tianjin Medical UniversityTianjinChina department of pathogen biology Tianjin Medical UniversityTianjinChina

Dear Editor,Although progressive respiratory failure is the primary cause of death in patients with COVID-19,thromboembolic complications further increased the mortality *** dysfunction,a pro-inflammatory and pro-coagulant state characterized by increased interaction with leukocytes and platelets,is reported to play a key role in COVID-19-associated thrombosis;1 however,its underlying mechanism remains unclear.

关键词： thrombosis mortality respiratory

Long noncoding RNAs in innate immunity

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Cellular & Molecular immunology 2016年第2期13卷 138-147页

作者： Yuan Zhang Xuetao Cao National Key Laboratory of Medical Molecular biology & department of immunology Institute of Basic Medical Sciences Chinese Academy of MedicalSciences Beijing 100005 China

Long noncoding RNAs （IncRNAs） have been shown to play important roles in immune cell development and immune responses through different mechanisms, such as dosage compensation, imprinting, enhancer function, and transcriptional regulation. Although the functions of most IncRNAs are unclear, some IncRNAs have been found to control transcriptional or post-transcriptional regulation of the innate and adaptive immune responses via new methods of protein-protein interactions or pairing with DNA and RNA. Interestingly, increasing evidence has elucidated the importance of IncRNAs in the interaction between hosts and pathogens. In this review, an overview of the IncRNAs modes of action, as well as the important and diversified roles of IncRNAs in immunity, are provided, and an emerging paradigm of IncRNAs in regulating innate immune responses is highlighted.

关键词： host-pathogen interaction innate immunity long noncoding RNA

Intravitreal bevacizumab versus triamcinolone acetonide for macular edema due to branch retinal vein occlusion： a matched study

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Chinese Medical Journal 2009年第22期122卷 2695-2699页

作者： HOU Jing TAO Yong JIANG Yan-rong LI Xiao-xin GAO Lei department of Ophthalmology Peking University People's Hospital Beijing 100044 China Institute of pathogen biology Chinese Academy of Medical.Sciences & Peking Union Medical College Beijing 100730 China

Background Branch retinal vein occlusion （BRVO） is a common retinal vascular disorder of the elderly and both intravitreal triamcinolone acetonide （TA） and intravitreal bevacizumab were reported to be effective. The purpose of this study was to compare intravitreal bevacizumab with intravitreal TA for the treatment of macular edema resulting from BRVO. Methods The retrospectively comparative interventional study included a bevacizumab group of 34 BRVO patients （1.25 mg bevacizumab） and a TA group of 34 BRVO patients （4.0 mg TA）, and the two groups were matched by baseline best corrected visual acuity （BCVA）. Examinations were designed to be carried out at 1 day, 3 days, 1 month, 2 months, 3 months, 6 months and 1 year after each injection. The mean follow-up was （148.43±130.56） days. Main outcome parameters were BCVA and morphometric measurements of the macula obtained by optical coherence tomography. Results In all follow-ups, the mean changes of BCVA （LogMAR） between two groups were not significantly different （P 〉0.10）. Similarly, the rates of patients who got BCVA improvement ≥2 lines or lost BCVA ≥2 lines were not significantly different, either （P 〉0.10）. In both groups, compared with baseline, the mean central macular thickness （CMT） got reduction from 4 weeks to 1 year after initial injection, however, which lost statistical significance at 6-month follow-up in TA group （P=0.25） and lost significance at 3-month and 6-month follow-up in bevacizumab group （P=0.07, 0.21）. The mean CMT between two groups differed at 3-month follow-up （P 〈0.01）, while almost kept parallel in other follow-ups （all P 〉0.40）. In TA group, retinal pigment epithelium tear occurred in 1 eye at 8 weeks after initial injection and 12 eyes （35.3%） got intraocular pressure 〉21 mmHg. In bevacizumab group, no severe complications were observed. Conclusion For BRVO, intravitreal bevacizumab versus intravitreal TA causes a similar increase in visual acuity and reduction of macular edema （excep

关键词： bevacizumab branch retinal vein occlusion triamcinolone acetonide

Small holes,big impact:Stomata in plant–pathogen–climate epic trifecta

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Molecular Plant 2024年第1期17卷 26-49页

作者： Shuguo Hou Olivier Rodrigues Zunyong Liu Libo Shan Ping He Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agriculture Sciences in WeifangWeifangShandong 261325China School of Municipal&Environmental Engineering Shandong Jianzhu UniversityJinanShandong 250101China Unitéde Recherche Physiologie Pathologie et Génétique VégétalesUniversitéde Toulouse Midi-PyrénéesINP-PURPAN31076 ToulouseFrance department of Molecular Cellularand Developmental BiologyUniversity of MichiganAnn ArborMI 48109USA

The regulation of stomatal aperture opening and closure represents an evolutionary battle between plants and pathogens,characterized by adaptive strategies that influence both plant resistance and pathogen *** ongoing climate change introduces further complexity,affecting pathogen invasion and host *** review delves into recent advances on our understanding of the mechanisms governing immunity-related stomatal movement and patterning with an emphasis on the regulation of stomatal opening and closure dynamics by pathogen patterns and host *** addition,the review explores how climate changes impact plant–pathogen interactions by modulating stomatal *** light of the pressing challenges associated with food security and the unpredictable nature of climate changes,future research in this field,which includes the investigation of spatiotemporal regulation and engineering of stomatal immunity,emerges as a promising avenue for enhancing crop resilience and contributing to climate control strategies.

关键词： stomatal movement stomatal patterning plant resistance pathogen virulence apoplastic immunity and climate change

Inhibition of HBV Replication by Delivering the Dual-gene Expression Vector pHsa-miR16-siRNA in HepG2.2.15 Cells

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2017年第6期37卷 828-832页

作者：魏巍汪速飞余冰倪明 department of pathogen biology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and Technology department of Infectious Disease Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

This study aimed to construct the dual-gene expression vector p Hsa-miR16-siRNA which can express human miR-16 and HBV X siRNA, and examine its regulatory effect on HBV gene expression in the HepG2.2.15 cell line. The expression vectors siR-1583 and pHsa-miR16-siRNA were designed and constructed. Hep G2.2.15 cells were transfected with the empty vector, siR-1583, pmiR-16 and pHsa-miR16-siRNA, respectively. ELISA was performed to measure the expression of HBsAg and HBeAg in the culture supernatant 48 and72 h post transfection. Fluorescence quantitative PCR was used to measure the HBV mRNA degradation efficiency and HBV DNA copy number. The results showed that the expression of HBV genes was significantly inhibited in Hep G2.2.15 cells transfected with siR-1583, pmiR-16 and pHsa-miR16-siRNA, respectively, when compared with that in cells transfected with the empty vectors, with the inhibitory effect of pHsa-miR16-siRNA being the most significant. ELISA showed that the inhibitory rates of HBs Ag and HBeAg in pHsa-miR16-siRNA transfected cells were correspondingly 87.3% and 85.0% at 48 h, and 88.6% and 86.5% at 72 h post transfection（P〈0.01 vs. control group）. RT-PCR showed that the level of HBV mRNA decreased by 80.2%（t=–99.22, P〈0.01）, the genomic HBV DNA by 92.8%（t=–73.06, P〈0.01）, and the supernatant of HBV DNA copy number by 89.8%（t=–47.13, P〈0.01） in pHsa-miR16-siRNA transfected group. It was suggested that the dual-gene expression vector pHsa-miR16-siRNA can inhibit the replication of HBV more efficiently than a single-gene expression vector.

关键词： dual expression vector HBV DNA HBV mRNA gene expression

An HBV-encoded miRNA activates innate immunity to restrict HBV replication

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Journal of Molecular Cell biology 2020年第4期12卷 263-276页

作者： Xiaoqing Zhao Lu Sun Ting Mu Jianying Yi Chaoqun Ma Hong Xie Min Liu Hua Tang Tianjin Life Science Research Center Tianjin Key Laboratory of Inflammation BiologyCollaborative Innovation Center of Tianjin for Medical EpigeneticsDepartment of Pathogen BiologySchool of Basic Medical SciencesTianjin Medical UniversityTianjin 300070China department of Laboratory Medicine General HospitalTianjin Medical UniversityTianjin 300070China

We previously identified that hepatitis B virus(HBV)encodes a microRNA(HBV-miR-3)that restrains HBV replication by targeting the HBV ***,whether HBV-miR-3 affects host innate immunity to modulate HBV replication remains ***,we examined the vital functions of HBV-miR-3 in the innate immune response after HBV *** found that HBV-miR-3 expression gradually increased in a dose-and time-dependent manner in HBV-infected HepG2-NTCP ***-miR-3 activated the JAK/STAT signaling pathway by downregulating SOCS5 in hepatocytes,thereby enhancing the IFN-induced anti-HBV *** addition,HBVmiR-3 in exosomes facilitated the Ml polarization of ***,exosomes containing HBV-miR-3 enhanced the secretion of IL-6 via inhibiting the SOCS5-mediated ubiquitination of *** short,these results demonstrate that HBV-miR-3 activates the innate immune response to restrain HBV replication by multiple pathways,which may suppress HBV-induced acute liver cell injury and affect the progression of persistent HBV infection.

关键词： hepatitis B virus HBV-miR-3 IFN M1 polarization IL-6

Tomato CYP94C1 inactivates bioactive JA-lle to attenuate jasmonate-mediated defense during fruit ripening

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Molecular Plant 2024年第4期17卷 509-512页

作者： Tianxia Yang Lei Deng Qinyang Wang Chuanlong Sun Muhammad Ali Fangming Wu Huawei Zhaj Qian Xu Peiyong Xin Shujing Cheng Jinfang Chu Tingting Huang Chang-Bao Li Chuanyou Li Key Laboratory of Seed Innovation Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China College of Life Sciences Shandong Agricultural UniversityTai'an 271018China Taishan Academy of Tomato Innovation Tai'an 271018China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China College of Horticulture Science and Engineering Shandong Agricultural UniversityTaian 271018China Institute of Vegetable Qingdao Academy of Agricultural SciencesQingdao 266100China Key Laboratory of biology and Genetic Improvement of Horticultural Crops(North China) Ministry of AgricultureBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry SciencesBejing 100097China Frontiers Science Center for Molecular Design Breeding(MOE) Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China

Dear Editor,Fruit rot caused by necrotrophic pathogens results in substantial reductions in fruit yield and revenues worldwide(Petrasch et al.,2019).A widespread phenomenon in fleshy fruit species is the susceptibility of ripe fruits to necrotrophs(Silva et al.,2021),which facilitates seed dispersal(Forlani et al.,2019)but causes severe post-harvest losses in *** most of the nutritional and sensory qualities of fruits are elaborated at the ripening stage(Liu et al.,2015),balancing fruit ripening and pathogen resistance to maintain fruit quality has proven to be challenging.A deeper understanding of the mechanisms underlying the increased susceptibility of fruits to necrotrophs during ripening could lead to new strategies for producing necrotrophy-resistant fruits without compromising ripening-relatedquality.

关键词： susceptibility maintain fruit

Alternative splicing patterns of doublesex reveal a missing link between Nix and doublesex in the sex determination cascade of Aedes albopictus

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Insect Science 2021年第6期28卷 1601-1620页

作者： Binbin Jin Yijie Zhao Yunqiao Dong Peiwen Liu Yan Sun Xiaocong Li Xin Zhang Xiao-Guang Chen Jinbao Gu department of pathogen biology Guangdong Provincial Key Laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical UniversityGuangzhou510515China Reproductive Medical Centre of Guangdong Women and Children Hospital Guangzhou511442China

Sexual development in insects is regulated by a complicated hierarchical cascade of sex *** primary signals are diverse,whereas the central nexus doublesex(dsx)gene is relatively conserved within the ***(Stegomyia)albopictus is an important vector with an extensive worldwide *** previously reported that *** dsx(Aalbdsx)yields one male-(Aalbdsx^(M))and three female-specific isoforms(Aalbdsx^(F1-3));however,the spatiotemporal expression profiles and mechanisms regulating sex-specific alternative splicing require further *** this study,we demonstrated that the Aalbdsx^(M) messenger RNA(mRNA)represents the default pattern when analyzed in human foreskin fibroblasts and HeLa *** combined reverse transcription polymerase chain reaction with RNA immunoprecipitation using specific antibodies against tagged *** male-determining factor AalNix and confirmed that AalNix indirectly regulates dsx pre-mRNA and regulates its alternative *** the early embryo stage(0-2 and 4-8 h),maternal dsx^(F) and default splicing dsx^(M) were detected in both sexes;the expression of dsx^(M) then decreased until sufficient AalNix transcripts accumulated in male embryos at 20-24 *** findings suggest that one or more potential dsx splicing enhancers can shift dsx^(M) to dsx^(F) in both sexes;however,the presence of Nix influences the function of this unknown splicing enhancer and ultimately leads to the formation of dsx^(M) in ***,our results provide important insight into the regulatory mechanism of dsx alternative splicing in the mosquito.

关键词： Aedes albopictus alternative splicing Doublesex\mosquito control sex determination

Synergistic and feedback signaling mechanisms in the regulation of inflammation in respiratory infections

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Cellular & Molecular immunology 2012年第2期9卷 131-135页

作者： Wenzhuo Y Wang Jae Hyang Lim Jian-Dong Li Center for Inflammation Immunity and Infection and Department of Biology Georgia State University Atlanta GA USA department of Microbiology and immunology University of Rochester Medical Center Rochester NY USA

Pneumonia, the most typical and frequent lower respiratory tract infection （LRTI）, is a leading cause of health problems in the United States. Bacteria represent the most prevailing cause of pneumonia in both children and adults. Although pneumonia with a single bacterial infection is common, a significant portion of patients with pneumonia is polymicrobial. This infection is often complexed with other physiological factors such as cytokines and growth factors. Nontypeable Haemophilus influenzae （NTHi） is the most frequently recovered Gram-negative bacterial pathogen in the respiratory system and induces strong inflammatory responses. NTHi also synergizes with other respiratory pathogens, such as Streptococcus pneumoniae and respiratory viruses and pro-inflammatory cytokines, such as tumor necrosis factor-alpha （TNF-α）. It is noteworthy that NTHi not only synergizes with growth factors such as transforming growth factor-beta （TGF-β）, but also utilizes growth factor receptors such as TGF-β receptor and epidermal growth factor receptor （EGFR）, to enhance inflammatory responses. Although appropriate inflammation is a protective response against invading pathogens, an uncontrolled inflammatory response is often detrimental to the host. Thus, inflammation must be tightly regulated. The human immune system has evolved strategies for controlling overactive inflammatory response. One such important mechanism is via regulation of negative feedback regulators for inflammation. CYLD, a multifunctional deubiquitinase, was originally reported as a tumor suppressor, but was recently identified as a negative regulator for nuclear factor-kappa B （NF-κB） signaling. It is induced by NTHi and TNF-α via a NF-κB-dependent mechanism, thereby serving as an inducible negative feedback regulator for tightly controlling inflammation in NTHi infection.

关键词： CYLD negative regulation NF-κB nontypeable Haemophilus influenzae synergistic regulation