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Dynamic changes in neuronal autophagy and apoptosis in the ischemic penumbra following permanent ischemic stroke

Neural Regeneration Research 2016年第7期11卷 1108-1114页

作者： Yi-hao Deng Hong-yun He Li-qiang Yang Peng-yue Zhang Department of Human Anatomy and Histoembryology Medical SchoolKunming University of Science and Technology

The temporal dynamics of neuronal autophagy and apoptosis in the ischemic penumbra following stroke remains unclear.Therefore,in this study,we investigated the dynamic changes in autophagy and apoptosis in the penumbra to provide insight into potential therapeutic targets for stroke.An adult Sprague-Dawley rat model of permanent ischemic stroke was prepared by middle cerebral artery occlusion.Neuronal autophagy and apoptosis in the penumbra post-ischemia were evaluated by western blot assay and immunofluorescence staining with antibodies against LC3-Ⅱ and cleaved caspase-3,respectively.Levels of both LC3-Ⅱ and cleaved caspase-3 in the penumbra gradually increased within 5 hours post-ischemia.Thereafter,levels of both proteins declined,especially LC3-Ⅱ.The cerebral infarct volume increased slowly 1–4 hours after ischemia,but subsequently increased rapidly until 5 hours after ischemia.The severity of the neurological deficit was positively correlated with infarct volume.LC3-Ⅱ and cleaved caspase-3 levels were high in the penumbra within 5 hours after ischemia,and after that,levels of these proteins decreased at different rates.LC3-Ⅱ levels were reduced to a very low level,but cleaved caspase-3 levels remained high 72 hours after ischemia.These results indicate that there are temporal differences in the activation status of the autophagic and apoptotic pathways.This suggests that therapeutic targeting of these pathways should take into consideration their unique temporal dynamics.

关键词： neuronal autophagy permanent infarct deficit caspase neurological targeting occlusion unclear

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Developmental distribution pattern of metabotropic glutamate receptor 5 in prenatal human hippocampus

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Neuroscience Bulletin 2012年第6期28卷 704-714页

作者： Pengbo Yang Junfeng Zhang Lingyu Zhao Qian Jiao Hui Jin Xinli Xiao Haixia Zhang Ming Hu HaiXia Lu Yong Liu Institute of Neurobiology Key Laboratory for Environment and Genes Related to Diseases of the Ministry of Education Department of Human Anatomy and Histoembryology Xi 'an Jiaotong University College of Medicine Department of Human Anatomy and Histoembryology Xi'an Jiaotong University College of MedicineXi 'an 710061China Department of Human Anatomy and Histoembryology Xi'an Jiaotong University College of Medicine

Objective Metabotropic glutamate receptor 5 （mGluR5） is concentrated in zones of active neurogenesis in the prenatal and postnatal rodent brain and plays an important role in the regulation of neurogenesis. However, little is known about mGluR5 in the prenatal human brain. Here, we aimed to explore the expression pattern and cellular distribution of mGluR5 in human fetal hippocampus. Methods Thirty-four human fetuses were divided into four groups according to gestational age： 9-11, 14-16, 22-24 and 32-36 weeks. The hippocampus was dissected out and prepared. The protein and mRNA expression of mGluR5 were evaluated by Western blot and immunohistochemistry or real-time PCR. The cellular distribution of mGluR5 was observed with double-labeling immunofluorescence. Results Both mGluR5 mRNA and protein were detected in the prenatal human hippocampus by real-time PCR and immunoblotting, and the expression levels increased gradually over time. The immunohistochemistry results were consistent with immunoblotting and showed that mGluR5 immunoreactivity was mainly present in the inner marginal zone （IMZ）, hippocampal plate （HP） and ventricular zone （VZ）. The double-labeling immunoftuorescence showed that mGluR5 was present in neural stem cells （nestin-positive）, neuroblasts （DCX-positive） and mature neurons （NeuN-positive）, but not in typical astrocytes （GFAP- positive）. The cells co-expressing mGluR5 and nestin were mainly located in the IMZ, HP and subplate at 11 weeks, all layers at 16 weeks, and CA 1 at 24 weeks. As development proceeded, the number of mGluR5/nestin double-positive cells decreased gradually so that there were only a handful of double-labeled cells at 32 weeks. However, mGluR5/DCX double-positive cells were only found in the HP, IZ and IMZ at 11 weeks. Conclusion The pattern ofmGluR5 expression by neural stern/progenitor cells, neuroblasts and neurons provides important anatomical evidence for the role of mGluR5 in the regulation of human hippocampal development.

关键词： metabotropic glutamate receptor 5 neurogenesis hippocampus human fetus

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Toxic effect of acrylamide on the development of hippocampal neurons of weaning rats

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Neural Regeneration Research 2017年第10期12卷 1648-1654页

作者： Sheng-min Lai Zi-ting Gu Meng-meng Zhao Xi-xia Li Yu-xin Ma Li Luo Jing Liu Department of Human Anatomy and Histoembryology School of Basic CoursesGuangdong Pharmaceutical UniversityGuangzhouGuangdong ProvinceChina

Although numerous studies have examined the neurotoxicity of acrylamide in adult animals,the effects on neuronal development in the embryonic and lactational periods are largely unknown.Thus,we examined the toxicity of acrylamide on neuronal development in the hippocampus of fetal rats during pregnancy.Sprague-Dawley rats were mated with male rats at a 1：1 ratio.Rats were administered 0,5,10 or 20 mg/kg acrylamide intragastrically from embryonic days 6–21.The gait scores were examined in pregnant rats in each group to analyze maternal toxicity.Eight weaning rats from each group were also euthanized on postnatal day 21 for follow-up studies.Nissl staining was used to observe histological change in the hippocampus.Immunohistochemistry was conducted to observe the condition of neurites,including dendrites and axons.Western blot assay was used to measure the expression levels of the specific nerve axon membrane protein,growth associated protein 43,and the presynaptic vesicle membrane specific protein,synaptophysin.The gait scores of gravid rats significantly increased,suggesting that acrylamide induced maternal motor dysfunction.The number of neurons,as well as expression of growth associated protein 43 and synaptophysin,was reduced with increasing acrylamide dose in postnatal day 21 weaning rats.These data suggest that acrylamide exerts dose-dependent toxic effects on the growth and development of hippocampal neurons of weaning rats.

关键词： nerve regeneration acrylamide hippocampus neurons developmental toxicity growth associated protein 43 synaptophysin weaning rats dentate gyrus protein developmental neurobiology neural regeneration

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Intellectual disability-related chromatin regulator Brpf1 affects electrophysiological feature of hippocampal neurons and related pathways

Intellectual disability-related chromatin regulator Brpf1 af...

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中国解剖学会2021年年会

作者： Xian Weiwei Yuan Xiangshan Cao Jingli Zhou Guomin You Linya department of human anatomy & histoembryology School of Basic Medical SciencesFudan University

Intellectual disability (ID) is a prevalent neurodevelopmental disorder,affecting 1%-2%of children.Recent researches revealed that haploinsufficiency of Brpfl could result in ID.Hippocampus plays important roles in learning and memory which are impaired in people with ID.To study the roles of Brpf1 in hippocampus, we investigated the morphology,electrophysiology and molecular mechanism in both cultured mouse hippocampal neurons by infecting with AAV-shBrpfl virus and in mouse model by injecting into hippocampal CA1 area with AAV-shBrpfl virus.

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The effects and mechanisms of TOX3 and TIGAR on the proliferation and differentiation of neural stem cells

The effects and mechanisms of TOX3 and TIGAR on the prolifer...

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中国解剖学会2021年年会

作者： Zhou Wenjuan Zhao Tiantian Du Jingyi Ji Guangyu Wu Dong Zhao Man Hao Aijun Department of Human Anatomy and Histoembryology School of Basic Medical ScienceShandong University

The formation of cerebral cortex is a highly ordered and strictly regulated development process,involving the production of various neural cells and the formation of nerve networks.The proliferation,differentiation,migration and stratification of NSCs are the cytological basis of cerebral cortex development.It is important to understand the potential molecular mechanism of proliferation and differentiation of NSCs.

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Brpf1 regulates the function of MGE-derived GABAergic interneurons

Brpf1 regulates the function of MGE-derived GABAergic intern...

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中国解剖学会2021年年会

作者： Jingli Cao Weiwei Xian Guomin Zhou Linya You department of human anatomy & histoembryology School of Basic Medical SciencesFudan University

Intellectual disability is a broadspectrum neurodevelopmental disorder characterized by marked impairment of intelligence and adaptive function.Impaired GABA neurotransmission is associated with mental disorders such as epilepsy and mental retardation,and patients with BRPF1 mutations were mentally retarded and often accompanied by epilepsy.Our research is to investigate the role of Brpf1 in the development and function of GABAergic interneurons.

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TIGAR promotes neural stem cell differentiation through acetyl-CoA-mediated histone acetylation

TIGAR promotes neural stem cell differentiation through acet...

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中国解剖学会2019年年会

作者： Wenjuan Zhou Tiantian Zhao Jingyi Du Guangyu Ji Xinyue Li Shufang Ji Wenyu Tian Xu Wang Aijun Hao Department of Human Anatomy and Histoembryology School of Basic Medical ScienceShandong University

＜正＞The metabolic transition from aerobic glycolysis to oxidative phosphorylation has been regarded as a hallmark of neuronal differentiation.Understanding what triggers metabolism reprogramming and how glucose meta...

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Effects of maternal high leptin levels on neurodevelopment of rat offspring

Effects of maternal high leptin levels on neurodevelopment o...

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中国解剖学会2019年年会

作者： Hu Xiaoxuan Ge Qian Cai Zhenlu Tan Ruolan Ma Ning Sun Meiqi Lu Haixia Institute ofNeurobiology Xi’an Jiaotong University department of human anatomy ＆ histoembryology Xi’an Jiaotong University

＜正＞Leptin plays a role in promoting angiogenesis,neurotrophy and neuroprotection.High level of leptin induced by maternal high fat （HF） diet during pregnancy and lactation may have profound impact on offspring’s...

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Analysis of cellular diversity and transcriptional regulatory network in human basal ganglia by single cell and bulk RNA sequencing

Analysis of cellular diversity and transcriptional regulator...

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中国解剖学会2021年年会

作者： Li Fengjiao Mohammadi H.B.Asad Yuan Xiangshan Xian Weiwei Liu Qiong Li Wensheng Zhou Guomin Wang Edwin You Linya department of human anatomy & histoembryology School of Basic Medical SciencesFudan University University of Calgary Cumming School of Medicine

The basal ganglia is a critical subcortical nuclei in several parallel and closed circuits,which mainly modulate thalamus-cortex activity.Dysfunction in any one of these loops connecting cortex can lead to movement and/or mood disorders.To understand cellular complexity,differential gene expression and transcriptional regulatory network of human basal ganglia under normal condition,

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Understandingnormal and diseased human basal ganglia using combinatory indexing single nuclei RNA sequencing

Understandingnormal and diseased human basal ganglia using c...

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中国解剖学会2021年年会

作者： Li Fengjiao Li Peilong Yuan Xiangshan Xian Weiwei Liu Qiong Li Wensheng Zhu Ying You Linya department of human anatomy & histoembryology School of Basic Medical SciencesFudan University Institutes of Brain Sciences Fudan University

With the advent of single cell technology and its rapid applications in the biomedical field,it becomes possible to identify,categorize and characterize each single cell in the brain.Shanghai Brain Bank is accumulating donorbrains very fast (estimately 80 brains till now) from people with normal aging or various neurodegenerative diseases.

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