检索结果-南通市图书馆

Two intestinal microbiota-derived metabolites, deoxycholic acid and butyrate, synergize to enhance host defense peptide synthesis and alleviate necrotic enteritis

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2024年第3期15卷 1158-1172页

作者： Dohyung M.Kim Jing Liu Melanie A.Whitmore Isabel Tobin Zijun Zhao Guolong Zhang department of Animal and food sciences Oklahoma State UniversityStillwaterOKUSA

Background Necrotic enteritis(NE)is a major enteric disease in poultry,yet effective mitigation strategies remain *** acid(DCA)and butyrate,two major metabolites derived from the intestinal microbiota,have independently been shown to induce host defense peptide(HDP)***,the potential synergy between these two compounds remains *** To investigate the possible synergistic effect between DCA and butyrate in regulating HDP synthesis and barrier function,we treated chicken HD11 macrophage cells and jejunal explants with DCA and sodium butyrate(NaB),either individually or in combination,for 24 ***,we performed RNA isolation and reverse transcrip-tion-quantitative PCR to analyze HDP genes as well as the major genes associated with barrier *** further determine the synergy between DCA and NaB in enhancing NE resistance,we conducted two independent trials with Cobb broiler *** each trial,the diet was supplemented with DCA or NaB on the day-of-hatch,followed by NE induction through sequential challenges with Eimeria maxima and Clostridium perfringens on d 10 and 14,*** recorded animal mortality after infection and assessed intestinal lesions on d *** impact of DCA and NaB on the microbiota in the ileum and cecum was evaluated through bacterial 16S rRNA gene *** We found that the combination of DCA and NaB synergistically induced multiple HDP genes in both chicken HD11 cells and jejunal ***,the gene for claudin-1,a major tight junction protein,also exhibited synergistic induction in response to DCA and ***,dietary supplementation with a combination of 0.75 g/kg DCA and 1 g/kg NaB led to a significant improvement in animal survival and a reduction in intestinal lesions compared to either compound alone in a chicken model of ***,the cecal microbiota of NE-infected chickens showed a marked decrease in SCFA-producing bacteria such as Bacteroides,F

关键词： Bile acids Host defense peptides Metabolites Microbiome Necrotic enteritis Short-chain fatty acids

Phenolic Profile and Volatiles of in vitro Propagated Lavandula angustifolia ***

在线全文

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2024年第3期93卷 427-444页

作者： Mir Abdullatif Yahya Fatma Tunali Dilek Killi Atalay Sökmen Biotechnology department Konya Food and Agriculture UniversityKonya42080Türkiye Plant Production and technologies department Konya Food and Agriculture UniversityKonya42080Türkiye

An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller,a medicinal aromatic plant that is a prominent source of volatile organic compounds(VOCs).Murashige and Skoog media were supplemented with various concentrations of Plant Growth Regulators(PGRs),and the growth parameters of the nodal segments were *** explants formed callus when they were supplemented with 2 mg/L of 6-Benzylaminopurine(BAP).The superior hormonal concentration of Murashige and Skoog(MS)media for the proliferation of shoots from callus cultures(39.33%)was 5 mg/L of 2-Isopentenyl adenine(2iP),and the favorable media for the growth of *** callus cultures was 1 and 2 mg/L of BAP,with a 98%for-mation rate in each *** callus cultures and in vitro propagated *** seedlings obtained from var-ious PGR concentrations of MS media were exposed to qualitative and quantitative analysis in terms of phenolic proﬁles,ﬂavonoids,High-performance liquid chromatography(HPLC)analysis of phenolic acids,and headspace-SPME analysis for *** analysis revealed that micropropagated seedlings grown in media containing 1 mg/L of 6-Furfurylaminopurine(KIN)accumulated the highest yield(11.95±0.01 mg GAE/g)of phenolic *** contrast,the lowest concentration(2.17±0.04 mg GAE/g)was detected in 0.5 mg/L of BAP+0.5 mg/L of Naphthaleneacetic acid(NAA)*** plantlets grown in 0.5 mg/L of BAP+0.5 mg/L of NAA media showed the highestﬂavonoid yield(31.67±0.06μg/g QE/g).In contrast,callus samples exhibited the lowest yield(11.59±0.02μg/g QE/g)ofﬂavonoids in MS media supplemented with a concentration of 0.5 mg/L of *** analysis revealed the variability of phenolic acid contents within the callus cultures as well as plantlets,with gallic acid,4-OH benzoic acid,chlorogenic acid,vanillic acid,caffeic acid,cinnamic acid,and rosmarinic acid being the prominent *** presence of twenty-two chemicals was revealed by headspace-SPME ***

关键词： Lavender indirect organogenesis micropropagation This

Utilization of pH-driven methods to fortify nanoemulsions with multiple polyphenols

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

food Science and Human Wellness 2024年第4期13卷 1943-1950页

作者： Hualu Zhou Bingjing Zheng David Julian McClements department of food Science and Technology College of Agricultural and Environmental SciencesUniversity of GeorgiaGriffinGA 30223USA Biopolymers and Colloids Laboratory Department of Food ScienceUniversity of MassachusettsAmherstMA 01003USA department of food Science&Bioengineering Zhejiang Gongshang UniversityHangzhou 310018China

Simple but effective methods are required to incorporate multiple bioactive polyphenols into delivery systems to increase their dispersibility,stability and *** developed and tested three p Hdriven protocols for creating nanoemulsions loaded with multiple lipophilic *** protocols differed in how the different polyphenols were incorporated into the *** impact of these three methods on the formation,properties,and gastrointestinal fate of nanoemulsions loaded with curcumin,resveratrol,and quercetin was *** three methods produced nanoemulsions with similar initial particle properties:droplet diameters(0.15,0.16,and 0.15μm)and zeta-potentials(–59,–58,and–58 m V),***,the average encapsulation efficiencies(82%,88%,and 61%),gastrointestinal stabilities(83%,97%,and 29%)and bioaccessibilities(77%,90%,and 73%)for curcumin,resveratrol,and quercetin were somewhat *** particular,more quercetin degradation occurred using the approach that held it under alkaline conditions for extended *** general,the p H-driven method provides researchers with a versatile approach of incorporating multiple polyphenols with different characteristics into functional food and beverages using a simple and inexpensive method.

关键词： Polyphenols pH-Driven encapsulation Nanoemulsions Gastrointestinal fate Functional foods

Methionine sources and genotype affect embryonic intestinal development, antioxidants, tight junctions, and growth-related gene expression in chickens

在线全文

学校读者我要写书评

暂无评论

Animal Nutrition 2024年第1期16卷 218-230页

作者： James K.Lugata Sawadi F.Ndunguru Gebrehaweria K.Reda Xénia E.Ozsváth Eszter Angyal Levente Czeglédi Gabriella Gulyás Renáta Knop János Oláh Zoltán Mészár Rita Varga Brigitta Csernus Csaba Szabó department of Animal Nutrition and Physiology Faculty of Agriculture and Food Sciences and Environmental Management University of Debrecen Faculty of Agriculture and food sciences and Environmental Management Doctoral School of Animal Science University of Debrecen department of Animal Husbandry Faculty of Agriculture and Food Sciences and Environmental Management University of Debrecen department of Evolutionary Zoology and Human Biology Faculty of Science and Technology University of Debrecen Institutes for Agricultural Research and Educational Farm University of Debrecen department of Anatomy Histology and Embryology Faculty of Medicine University of Debrecen

Methionine(Met) is an essential and first limiting amino acid in the poultry diet that plays a significant role in chicken embryonic development and growth. The present study examined the effect of in ovo injection of DL-Met and L-Met sources and genotypes on chicken embryonic-intestinal development and health. Fertilized eggs of the two genotypes, TETRA-SL layer hybrid(TSL)—commercial layer hybrid and Hungarian Partridge colored hen breed(HPC)—a native genotype, were randomly distributed into four treatments for each genotype. The treatment groups include the following: 1) control non-injected eggs(NoIn); 2) saline-injected(SaIn); 3) DL-Met injected(DLM); and 4) L-Met injected(LM). The in ovo injection was carried out on 17.5 d of embryonic development; after hatching, eight chicks per group were sacrificed, and the jejunum was extracted for analysis. The results showed that both DLM and LM groups had enhanced intestinal development as evidenced by increased villus width, villus height, and villus area(P < 0.05) compared to the control. The DLM group had significantly reduced crypt depth, glutathione(GSH) content, glutathione S-transferase 3 alpha(GST3), occludin(OCLN) gene expression and increased villus height to crypt depth ratio in the TSL genotype than the LM group(P < 0.05). The HPC genotype has overexpressed insulin-like growth factor 1(IGF1) gene, tricellulin(MD2), occludin(OCLN),superoxide dismutase 1(SOD1), and GST3 genes than the TSL genotype(P < 0.05). In conclusion, these findings showed that in ovo injection of Met enhanced intestinal development, and function, with genotypes responding differently under normal conditions. Genotypes also influenced the expression of intestinal antioxidants, tight junction, and growth-related genes.

关键词： DL-Methionine L-Methionine In ovo injection Intestinal development Poultry

Intestinal anti-inflammatory effect of a plant sterol food supplement in experimental murine colitis and cell co-culture models

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

food Science and Human Wellness 2024年

作者： Mussa Makran Antonio Cilla Rosa María Giner María Carmen Recio Luisa Tesoriere Alessandro Attanzio Guadalupe Garcia-Llatas Nutrition and food Science Area， Faculty of Pharmacy and food sciences， University of Valencia.Av.Vicente Andrés Estellés s/n department of Pharmacology， Faculty of Pharmacy and food sciences， University of Valencia.Av.Vicente Andrés Estellés s/n department of Biological， Chemical and Pharmaceutical sciences and technologies， University of Palermo

The potential of a plant sterol food supplement （PS-FS） in addressing intestinal inflammation in vivo and in vitro was evaluated. As in vivo model， C57BL/6J mice were exposed to 1.5% dextran sulfate sodium in three 5-day periods， with 10-day rest intervals in between， daily reciving PS-FS （35 mg PS/kg）. The in vitro approach involved a bi-cameral system with 8-day-differentiated Caco-2 （apical） and RAW264.7 cells （basolateral）. The bioaccessible fraction of PS-FS， obtained after INFOGEST 2.0 simulated gastrointestinal digestion， was added to the apical part （90 min）， followed by stimulation with lipopolysaccharide （1 μg/mL， 24 h）. PS-FS alleviated rectal bleeding and rebalanced pro- (tumor necrosis factor α， interleukin （IL）-6， and IL-8) and anti-inflammatory cytokines （IL-10）. Additionally， PS-FS ameliorated histopathological damage and enhancing occludin expression. A reduction in oxidative stress was evidenced by decreased myeloperoxidase activity and reactive oxygen species production. The anti-inflammatory mechanism included suppressing cyclooxygenase 2 expression， reducing prostaglandin E2production， and inhibiting the phosphorylation and nuclear translocation of the nuclear factor κB p65 subunit. This study reveals the potential of PS-FS as a therapeutic intervention for colitis. The alignment between in vivo and in vitro outcomes substantiates the appropriateness of the co-culture model to evaluate the anti-inflammatory activity of foods.

关键词：

Exergy Analysis of Charge and Discharge Processes of Thermal Energy Storage System with Various Phase Change Materials:A Comprehensive Comparison

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Thermal Science 2024年第2期33卷 509-521页

作者： Mojtaba TAHERI Fathollah POURFAYAZ Roghayeh HABIBI Akbar MALEKI department of Renewable Energies and Environment Faculty of New Sciences and TechnologiesUniversity of TehranTehran***Iran Faculty of Mechanical Engineering Shahrood University of TechnologyShahrood***Iran

Thermal energy storage(TES) is of great importance in solving the mismatch between energy production and *** this regard,choosing type of Phase Change Materials(PCMs) which are widely used to control heat in latent thermal energy storage systems,plays a vital role as a means of TES ***,this field suffers from lack of a comprehensive investigation on the impact of various PCMs in terms of *** address this issue,in this study,in addition to indicating the melting temperature and latent heat of various PCMs,the exergy destruction and exergy efficiency of each material are estimated and compared with each ***,in the present work the impact of PCMs mass and ambient temperature on the exergy efficiency is *** results proved that higher latent heat does not necessarily lead to higher exergy ***,to obtain a suitable exergy efficiency,the specific heat capacity and melting temperature of the PCMs must also be *** to the results,LiF-CaF_(2)(80.5%:19.5%,mass ratio) mixture led to better performance with satisfactory exergy efficiency(98.84%) and notably lower required mass compared to other ***,the highest and lowest exergy destruction are belonged to GR25 and LiF-CaF_(2)(80.5:19.5) mixture,respectively.

关键词： exergy analysis phase change materials thermal energy storage exergy efficiency

Evaluation of ruminal methane and ammonia formation and microbiota composition as affected by supplements based on mixtures of tannins and essential oils using Rusitec

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2024年第4期15卷 1607-1622页

作者： Giulia Foggi Melissa Terranova Matteo Daghio Sergej L.Amelchanka Giuseppe Conte Simon Ineichen Monica Agnolucci Carlo Viti Alberto Mantino Arianna Buccioni Michael Kreuzer Marcello Mele department of Agriculture Food and Environment(DAFE)University of Pisa56124 PisaItaly AgroVet‑Strickhof ETH ZurichLindauSwitzerland department of Agriculture FoodEnvironment and ForestryUniversity of FlorenceFlorence 50144Italy Centro Di Ricerche Agro‑Ambientali“E.Avanzi” University of PisaPisa 56122Italy School of Agricultural Forest and Food Sciences HAFLBern University of Applied SciencesZollikofenSwitzerland Institute of Agricultural sciences ETH ZurichLindauSwitzerland

Background Dietary supplements based on tannin extracts or essential oil compounds(EOC)have been repeatedly reported as a promising feeding strategy to reduce the environmental impact of ruminant husbandry.A previous batch culture screening of various supplements identified selected mixtures with an enhanced potential to mitigate ruminal methane and ammonia *** these,Q-2(named after quebracho extract and EOC blend 2,composed of carvacrol,thymol,and eugenol)and C-10(chestnut extract and EOC blend 10,consisting of oregano and thyme essential oils and limonene)have been investigated in detail in the present study with the semi-continuous rumen simulation technique(Rusitec)in three independent *** this purpose,Q-2 and C-10,dosed according to the previous study,were compared with a non-supplemented diet(negative control,NC)and with one supplemented with the commercial EOC-based Agolin^(R) Ruminant(positive control,PC).Results From d 5 to 10 of fermentation incubation liquid was collected and analysed for pH,ammonia,protozoa count,and gas *** residues were collected for the determination of ruminal *** d 10,samples of incubation liquid were also characterised for bacterial,archaeal and fungal communities by high-throughput sequencing of 16S rRNA and 26S ribosomal large subunit gene *** of the duration of the fermentation period,Q-2 and C-10 were similarly efficient as PC in mitigating either ammonia(-37%by Q-2,-34%by PC)or methane formation(-12%by C-10,-12%by PC).The PC was also responsible for lower feed degradability and bacterial and fungal richness,whereas Q-2 and C-10 effects,particularly on microbiome diversities,were limited compared to *** All additives showed the potential to mitigate methane or ammonia formation,or both,in vitro over a period of 10 ***,several differences occurred between PC and Q-2/C-10,indicating different mechanisms of *** pronounced defaunation caused by PC a

关键词： Additive Bacteria Continuous culture Degradability Fermentation Fungi Greenhouse gas In vitro Microbiome Rumen

Impact of umbelliprenin-containing niosome nanoparticles on VEGF-A and CTGF genes expression in retinal pigment epithelium cells

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2024年第1期17卷 7-15页

作者： Farzad Dastaviz Akram Vahidi Teymoor Khosravi Ayyoob Khosravi Mehdi Sheikh Arabi Abouzar Bagheri Mohsen Rashidi Morteza Oladnabi Stem Cell Research Center Golestan University of Medical SciencesGorgan ***Iran department of Medical Genetics School of Advanced Technologies in MedicineGolestan University of Medical SciencesGorgan ***Iran Student Research Committee Golestan University of Medical SciencesGorgan ***Iran department of Molecular Medicine Faculty of Advanced Medical TechnologiesGolestan University of Medical SciencesGorgan ***Iran department of Medical Nanotechnology Faculty of Advanced Medical TechnologiesGolestan University of Medical SciencesGorgan ***Iran department of Clinical Biochemistry and Genetics Faculty of MedicineMazandaran University of Medical SciencesSari ***Iran department Pharmacology Faculty of MedicineMazandaran University of Medical SciencesSari ***Iran Gorgan Congenital Malformations Research Center Golestan University of Medical SciencesGorgan ***Iran

AIM:To investigate the impact of niosome nanoparticles carrying umbelliprenin(UMB),an anti-angiogenic and anti-inflammatory plant compound,on the expression of vascular endothelial growth factor(VEGF-A)and connective tissue growth factor(CTGF)genes in a human retinal pigment epithelium(RPE)-like retina-derived cell ***:UMB-containing niosomes were created,optimized,and ***-like cells were treated with free UMB and UMB-containing *** IC_(50)values of the treatments were determined using an MTT *** expression of VEGF-A and CTGF was evaluated using real-time polymerase chain reaction after RNA extraction and cDNA ***’characteristics,including drug entrapment efficiency,size,dispersion index,and zeta potential were *** UMB had an IC_(50)of 96.2μg/mL,while UMB-containing niosomes had an IC_(50)of 25μg/***:Treatment with UMB-containing niosomes and free UMB resulted in a significant reduction in VEGF-A expression compared to control cells(P=0.001).Additionally,UMB-containing niosomes demonstrated a significant reduction in CTGF expression compared to control cells(P=0.05).However,there was no significant reduction in the expression of both genes in cells treated with free ***:Both free UMB and niosome-encapsulated UMB inhibits VEGF-A and CTGF genes ***,the latter demonstrates significantly greater efficacy,potentially due to the lower UMB dosage and gradual *** findings have implications for anti-angiogenesis therapeutic approaches targeting age-related macular degeneration.

关键词： umbelliprenin niosome age-related macular degeneration vascular endothelial growth factor-A connective tissue growth factor

Effect of in ovo feeding of xylobiose and xylotriose on plasma immunoglobulin, cecal metabolites production, microbial ecology, and metabolic pathways in broiler chickens

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2024年第4期15卷 1578-1589页

作者： Razib Das Pravin Mishra Birendra Mishra Rajesh Jha department of Human Nutrition Food and Animal SciencesCollege of Tropical Agriculture and Human ResourcesUniversity of Hawaii at ManoaHonoluluHI 96822USA

Background Dietary supplementation of xylooligosac charides(XOS) has been found to influence gut health by manipulating cecal microbiota and producing microbe-origin *** no study investigated and compared the effect of in ovo feeding of xylobiose(XOS2) and xy lotriose(XOS3) in *** study investigated the effect of in ovo feeding of these XOS compounds on post-hatch gut health parameters in chickens.A total of 144 fertilized chicken eggs were divided into three groups:a) non-injected control(CON),b) XOS2,and c) *** the 17^(th) embryonic day,the eggs of the XOS2 and XOS3 groups were injected with 3 mg of XOS2 and XOS3 diluted in 0.5 mL of 0.85% normal saline through the amniotic *** hatching,the chicks were raised for 21 *** was collected on d 14 to measure plasma *** digests were collected for measuring short-chain fatty acids(SCFA) on d 14 and 21,and for microbial ecology and microbial metabolic pathway analyses on d 7 and *** The results were considered significantly different at P<*** quantified plasma IgA and IgG on d 14chickens,revealing no differences among the *** chromatography results showed no significant differences in the concentrations of cecal SCFAs on d 14 but significant differences on d ***;the SCFA concentrations were lower in the XOS3 than in the CON group on d *** cecal metagenomics data showed that the abundance of the family Clostridiaceae significantly decreased on d 7,and the abundance of the family Oscillospiraceae increased on d 21 in the XOS2 compared to the *** was a reduction in the relative abundance of genus Clostridium sense stricto 1 in the XOS2 compared to the CON on d 7 and the genus Ruminococcus,torques in both XOS2 and XOS3 groups compared to the CON on d *** XOS2 and XOS3 groups reduced the genes for chondroitin sulfate degradation Ⅰ and L-histidine degradation Ⅰpathways,which contribute to improved gut health,respectivelyc in the micr

关键词： Broiler In ovo Metagenomics Prebiotic Xylooligosaccharides