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Purines Change at Acupoints along the Pericardium Meridian in Healthy and Myocardial Ischemic Rats

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Chinese Journal of Integrative Medicine 2019年第4期25卷 285-291页

作者： ZHOU Yu-mei ZHUANG Yi CAI Ding-jun LV Pei-ran ZHOU Jie WAN Min REN Yu-lan LIANG Fan-rong department of Acupuncture and Tuina School Chengdu University of Traditional Chinese MedicineChengdu(610075)China The Second clinical Medical College of Nanjing University of Chinese Medicine Nanjing(210046)China

Objective: To quantify the purine concentrations of the acupoints along the pericardium and nonpericardium meridians under healthy and myocardial ischemia conditions to investigate the relationship between acupoint purine change and body functional status in ***: A total of 70 rats underwent an operation for myocardial ischemia,while 40 of them *** were randomly assigned to the following 5 subgroups:Neiguan(PC 6),Quze(PC 3),Tianquan(PC 2),Quchi(LI 11),and Jianyu(LI 15).Simultaneously,another40 healthy rats were also randomized into the same 5 subgroups as the control *** tissue ?uids at the acupoints were collected by microdialysis for 30 ***,the concentration of adenosine triphosphate(ATP),adenosine diphosphate(ADP),adenosine monophosphate(AMP),and adenosine(ADO) were quanti?ed using the high-performance liquid chromatography ***: Compared with the healthy group,the ADO at PC 6(P=0.012),PC 3(P=0.038),PC 2(P=0.024),and LI 15(P=0.042) obviously increased in the model group,while no signi?cant difference was observed at LI 11(P=0.201).However,ATP,ADP,and AMP manifested no significant changes in these areas,except for ATP at LI 15(P=0.036).Conclusions: Myocardial ischemia could induce an increase in ADO at acupoints of the upper arm and shoulder area,suggesting that the body functional status could affect the responsiveness of *** status of these acupoints could be pathogenically activated by disease,and distribution following some speci?c courses.

关键词： acupoint myocardial ischemia pericardium meridian purine adenosine microdialysis high-performance liquid chromatography

Spinal cord conduits for spinal cord injury regeneration

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Engineered Regeneration 2023年第1期4卷 68-80页

作者： Yanyu Ma Quanchi Chen Wenhan Li Haiwen Su Song Li Yitong Zhu Jie Zhou Zhenhua Feng Zhen Liu Saihu Mao Yong Qiu Huan Wang Zezhang Zhu Division of Spine Surgery Department of Orthopedic SurgeryAffiliated Drum Tower HospitalMedical SchoolNanjing UniversityNanjing210008China Division of Spine Surgery Department of Orthopedic SurgeryAffiliated Drum Tower Hospital Clinical College of Nanjing University of Chinese MedicineNanjing210008China department of Nephrology Affiliated Drum Tower Hospital Clinical College of Nanjing University of Chinese MedicineNanjing210008China Division of Spine Surgery Department of Orthopedic SurgeryNanjing Drum Tower Hospital Clinical College of Nanjing Medical UniversityNanjing210008China The Eighth Affiliated Hospital Sun Yat-Sen UniversityShenzhen518033China

Spinal cord injury(SCI),which causes irreversible damage in both sensory and motor function,is considered an insurmountable challenge in the field of *** previous researchers have developed many kinds of biomaterials for SCI,and spinal cord conduits(SCCs)are an important classification for bridging spinal cord tissues while performing their corresponding *** this review,we first describe the original sources of the different polymers that determine the properties of the different ***,we focus on the different types of crosslinking methods used for preparing ***,various practical applications and therapeutic effects of SCCs are summarized and ***,we conclude the existing limitations of current *** hope that this paper will serve as a further inspiration for the development of SCCs in future.

关键词： Spinal cord injury Neuron regeneration Hydrogel Spinal cord conduit Tissue engineering

Epalrestat protects against diabetic peripheral neuropathy by alleviating oxidative stress and inhibiting polyol pathway

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Neural Regeneration Research 2016年第2期11卷 345-351页

作者： Qing-rong Li Zhuo Wang Wei Zhou Shou-rui Fan Run Ma Li Xue Lu Yang Ya-shan Li Hong-li Tan Qi, ng-hua Shao Hong-ying Yang Second Affiliated Hospital of Kunming Medical University Department of Clinical Laboratory Kunming Yunnan Province China Third People's Hospital of Yunnan Province Kunming Yunnan Province China

Epalrestat is a noncompetitive and reversible aldose reductase inhibitor used for the treatment of diabetic neuropathy. This study assumed that epalrestat had a protective effect on diabetic peripheral nerve injury by suppressing the expression of aldose reductase in peripheral nerves of diabetes mellitus rats. The high-fat and high-carbohydrate model rats were established by intraperitoneal injection of streptozotocin. Peripheral neuropathy occurred in these rats after sustaining high blood glucose for 8 weeks. At 12 weeks after streptozotocin injection, rats were intragastrically administered epalrestat 100 mg/kg daily for 6 weeks. Transmission electron microscope revealed that the injuries to myelinated nerve fibers, non-myelinated nerve fibers and Schwann cells of rat sciatic nerves had reduced compared to rats without epalrestat administuation. Western blot assay and immunohistochemical results demonstrated that after intervention with epalrestat, the activities of antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase gradually increased, but aldose reductase protein expression gradually diminished. Results confirmed that epalrestat could protect against diabetic peripheral neuropathy by relieving oxidative stress and suppressing the polyol pathway.

关键词： nerve regeneration peripheral nerve injury streptozotocin reactive oxygen species diabetic neuropathy oxidative stress aldosereductase antioxidant enzymes polyol pathway aldose reductase inhibitor superoxide dismutase catalase glutathione peroxidase rats NSFCgrant neural regeneration

Trigonella foenum-graecum seed extract modulates expression of lipid metabolism-related genes in HepG2 cells

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Asian Pacific Journal of Tropical Biomedicine 2019年第6期9卷 240-248页

作者： Maryam Mohammad-Sadeghipour Mehdi Mahmoodi Soudeh Khanamani Falahati-pour Alireza Khoshdel Mohammad Ali Fahmidehkar Mohammad Reza Mirzaei Mojgan Noroozi Karimabad Mohammad RezaHajizadeh department of clinical Biochemistry Faculty of MedicineRafsanjan University of Medical SciencesRafsanjanIran Molecular Medicine Research Center Inistitute of Basic Medical Sciences ResearchRafsanjan University of Medical SciencesRafsanjanIran department of clinical Biochemistry Afzalipoor Faculty of MedicineKerman University of Medical SciencesKermanIran Pistachio Safety Research Center Rafsanjan University of Medical SciencesRafsanjanIran department of clinical Biochemistry Faculty of Medicineand Pistachio Safety Research CenterRafsanjan University of Medical SciencesRafsanjanIran department of Laboratory Sciences School of Paramedical SciencesTorbat Heydariyeh University of Medical SciencesTorbat HeydariyehIran

Objective:To investigate anti-dyslipidemic effects of hydroalcoholic fenugreek seed extracts,diosgenin,and 4-OH-Ile on HepG2 cell ***:HepG2 cells were treated with hydroalcoholic fenugreek seed extracts,diosgenin,4-OH-Ile,and ***20 was calculated using the MTT *** cells were then pretreated with IC20 concentrations for 24 and 48 *** time PCR was employed to measure expression of liver X receptor alpha(LXR a),sterol regulatory element-binding protein-1 C(SREBP-1 C),acetyl-CoA carboxylase(ACC),fatty acid synthase(FAS),fibroblast growth factor 21(FGF21),peroxisome proliferator-activated receptor gamma(PPAR γ),and lowdensity lipoprotein receptor(LDLR).Results:The results showed that LXR α(P=0.003,P<0.001).,SREBP-1 C(P<0.001,P<0.001),ACC(P=0.002,P=0.006),and FAS(P<0.001,P<0.001)were downregulated significantly,while FGF21(P<0.001,P<0.001),PPAR γ(P=0.004,P<0.001),and LDLR(P<0.001,P<0.001)were upregulated significantly in HepG2 cells treated with the IC20 of hydroalcoholic fenugreek seed extracts,diosgenin,4-OH-Ile,and orlistat in 24 and 48 h,***:Hydroalcoholic fenugreek seed extracts,diosgenin,and 4-OH-Ile significantly modulate the expression of some important lipid metabolism related genes,which is similar to *** foenum.-graecum seed extract or its derivatives should be further investigated for their dyslipidemia effects and its complications.

关键词： Fenugreek seed extract Diosgenin 4-Hydroxy-L-isoleucine Dyslipidemia Obesity

Hydrogen sulfide suppresses transforming growth factor-β1-induced differentiation of human cardiac fibroblasts into myofibroblasts

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Science China(Life Sciences) 2015年第11期58卷 1126-1134页

作者： ZHANG YouEn WANG JiaNing LI Hua YUAN LiangJun WANG Lei WU Bing GE JunBo Institute of clinical Medicine and department of Cardiology Renmin Hospital Hubei University of Medicine Shanghai Institute of Cardiovascular Diseases Zhongshan Hospital Fudan University Institutes of Biomedical Sciences Fudan University

In heart disease, transforming growth factor-β1 （TGF-β1） converts fibroblasts into myofibroblasts, which synthesize and se- crete fibrillar type I and III collagens. The purpose of the present study was to investigate how hydrogen sulfide （HzS） sup- presses TGF-~l-induced differentiation of human cardiac fibroblasts to myofibroblasts. Human cardiac fibroblasts were se- rum-starved in fibroblast medium for 16 h before exposure to TGF-β1 （10 ng mL-1） for 24 h with or without sodium hydrosul- fide （NariS, 100 μmol L-1, 30 min pretreatment） treatment. NariS, an exogenous HzS donor, potently inhibited the prolifera- tion and migration of TGF-β1-induced human cardiac fibroblasts and regulated their cell cycle progression. Furthermore, NariS treatment led to suppression of fibroblast differentiation into myofibroblasts, and reduced the levels of collagen, TGF-β1, and activated Smad3 in TGF-β1-induced human cardiac fibroblasts in vitro. We therefore conclude that H2S sup- presses TGF-β1-stimulated conversion of fibroblasts to myofibroblasts by inhibiting the TGF-β1/Smad3 signaling pathway, as well as by inhibiting the proliferation, migration, and cell cycle progression of human cardiac myofibroblasts. These effects of H2S may play significant roles in cardiac remodeling associated with heart failure.

关键词： human cardiac fibroblasts hydrogen sulfide transforming growth factor β1 myofibroblasts

Osteopontin(OPN)alleviates the progression of osteoarthritis by promoting the anabolism of chondrocytes

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Genes & Diseases 2023年第4期10卷 1714-1725页

作者： Wei Luo Zili Lin Yuhao Yuan Ziyi Wu Wei Zhong Qing Liu department of Orthopaedics Xiangya HospitalCentral South UniversityChangshaHunan 410008China National clinical Research Center for Geriatric Disorders Xiangya HospitalChangshaHunan 410008China

Osteoarthritis(OA)is a chronic debilitating joint disease,characterized by degen-eration of the cartilage and loss of the cartilage matrix,and it is clinically manifested as joint ***(OPN)is a glycoprotein that is abnormally expressed in the bone and carti-lage tissues and plays a vital role in various pathological processes such as the osteoarthritic inflammatory response and endochondral *** focus of our study is to investigate the therapeutic potential and specific role of OPN in *** morphological comparisons,we found that the cartilage was severely worn-out and there was a significant loss of the cartilage matrix in ***,CD44,and hyaluronic acid(HA)synthase 1(HAS1)were highly expressed,and the anabolism of HA was significantly higher in the OA chondrocytes than in the control ***,we treated the OA chondrocytes with small interfering RNA(siRNA)targeting OPN,recombinant human OPN(rhOPN),and a combination of rhOPN and anti-CD44 ***,in vivo experiments were performed in *** found that OPN up-regulated the expression of downstream HAS1 and increased the anabolism of HA through CD44 protein expression in OA mice compared with those in control ***,intra-articular injection of OPN in mice with OA significantly inhibited OA *** summary,OPN ini-tiates an intracellular cascade via CD44 which results in an anabolic increase in HA levels,thereby inhibiting OA ***,OPN is a promising therapeutic agent in precision treatment of OA.

关键词： Cartilage degeneration CD44 Hyaluronic acid Osteoarthritis Osteopontin

Exogenous acid fibroblast growth factor inhibits ischemia-reperfusion-induced damage in intestinal epithelium via regulating P53 and P21WAF-1 expression

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World Journal of Gastroenterology 2005年第44期11卷 6981-6987页

作者： Wei Chen Xiao-Bing Fu Shi-Li Ge Tong-Zhu Sun Zhi-Yong Sheng Key Research Laboratory of Wound Repair Burns Institute 304^th Clinical Department General Hospital of PLABeijing 100037 China Institute of Radiation Medicine Beijing 100850 China

AIM： To detect the effect of acid fibroblast growth factor （aFGF） on P53 and P21WAF-1 expression in rat intestine after ischemia-reperfusion （I-R） injury in order to explore the protective mechanisms of aFGF. METHODS： Hale rats were randomly divided into four groups, namely intestinal ischemia-reperfusion group （R）, aFGF treatment group （A）, intestinal ischemia group （I）, and sham-operated control group （C）. In group I, the animals were killed after 45 min of superior mesenteric artery （SHA） occlusion. In groups R and A, the rats sustained for 45 min of SHA occlusion and were treated with normal saline （0.15 mL） and aFGF （20 μg/kg, 0.15 mL）, then sustained at various times for up to 48 h after reperfusion. In group C, SHA was separated, but without occlusion. Apoptosis in intestinal villi was determined with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling technique （TUNEL）. Intestinal tissue samples were taken not only for RT- PCR to detect P53 and P21WAF-1 gene expression, but also for immunohistochemical analysis to detect P53 and P21WAF-1 protein expression and distribution. RESULTS： In histopathological study, ameliorated intestinal structures were observed at 2, 6, and 12 h after reperfusion in A group compared to R group. The apoptotic rates were （41.17±3.49）%, （42.83±5.23）%, and （53.33±6.92）% at 2, 6, and 12 h after reperfusion, respectively in A group, which were apparently lower than those in R group at their matched time points （50.67±6.95）%, （54.17±7.86）%, and （64.33±6.47）%, respectively, （P〈0.05））. The protein contents of P53 and P21WAF-1 were both significantly decreased in A group compared to R group （P〈0.05） at 2-12 h after reperfusion, while the mRNA levels of P53 and P21VVAF-1 in A group were obviously lower than those in R group at 6-12 h after reperfusion （P〈0.05）. CONCLUSION： P53 and P21WAF-1 protein accumulations are associated with intestinal barrier injury induced by I-R insult, while intravenous aFGF can all

关键词： Acid fibroblast growth factor Ischemia Reperfusion P53 gene P21WAF-1 gene

初级保健实验医学中的病例高血压患者的肾功能监测

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英国医学杂志中文版 2007年第1期10卷 56-58页

作者： Una Martin Jamie J Coleman 龙泉 department of clinical pharmacology Division of Medical SciencesUniversity of BirminghamBirmingham B15 2TH senior lecturer in clinical pharmacology department of clinical pharmacology Division of Medical SciencesUniversity of BirminghamBirmingham B15 2TH specialist registrar in clinical pharmacology

本文要点在高血压治疗前、治疗中监测肾功能和电解质可以为潜在的问题提供警示,有助于避免副反应生化参数的早期改变可能很重要。

关键词：肾功能监测高血压患者实验医学初级保健病例肾小球滤过率高血压治疗药物副作用

The role of transcriptional factor D-site-binding protein in circadian CCL2 gene expression in anti-Thy1 nephritis

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Cellular & Molecular Immunology 2019年第9期16卷 735-745页

作者： Yang Lu Yan Mei Lei Chen Lingling Wu Xu Wang Yingjie Zhang Bo Fu Xizhao Chen Yuansheng Xie Guangyan Cai Xueyuan Bai Qinggang Li Xiangmei Chen department of Nephrology Chinese PLA General HospitalChinese PLA Institute of NephrologyState Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney DiseasesBeijingChina

Mesangial proliferative glomerulonephritis(MsPGN)is an inflammatory disease,but both the nature of disease progression and its regulation remain *** the present study,we monitored the course of anti-Thy1 nephritis from days 1 to 5 and established gene expression profiles at each time point using microarrays to explore the development of *** to the gene expression profiles,macrophage infiltration(triggered by CCL2 activation)was evident on day 1 and enhanced inflammation over the next few *** screened for genes with expression levels similar to CCL2 and found that the upregulation of the circadian gene albumin D-site-binding protein(DBP)was involved in CCL2 activation in mesangial *** importantly,CCL2 expression showed oscillatory changes similar to DBP,and DBP induced peak CCL2 expression at 16:00 a clock on day 1 in the anti-Thy1 nephritis *** knocked down DBP through transfection with a small interfering RNA(siRNA)and used RNA sequencing to identify the DBP-regulated TNF-α-CCL2 *** performed chromatin immunoprecipitation sequencing(ChIP-Seq)and the dual luciferase assay to show that DBP bound to the TRIM55 promoter,regulating gene expression and in turn controlling the TNF-α-CCL2 *** conclusion,DBP-regulated circadian CCL2 expression by the TRIM55-TNF pathway in injured mesangial cells at an early stage,which promoted macrophage recruitment and in turn triggered infiltration and inflammation in a model of anti-Thy1 nephritis.

关键词： Mesangial proliferative glomerulonephritis DBP CCL2 Trim 55 Macrophage