Trigonella foenum-graecum seed extract modulates expression of lipid metabolism-related genes in HepG2 cells

作     者：Maryam Mohammad-Sadeghipour Mehdi Mahmoodi Soudeh Khanamani Falahati-pour Alireza Khoshdel Mohammad Ali Fahmidehkar Mohammad Reza Mirzaei Mojgan Noroozi Karimabad Mohammad RezaHajizadeh 

作者机构：Department of Clinical BiochemistryFaculty of MedicineRafsanjan University of Medical SciencesRafsanjanIran Molecular Medicine Research CenterInistitute of Basic Medical Sciences ResearchRafsanjan University of Medical SciencesRafsanjanIran Department of Clinical BiochemistryAfzalipoor Faculty of MedicineKerman University of Medical SciencesKermanIran Pistachio Safety Research CenterRafsanjan University of Medical SciencesRafsanjanIran Department of Clinical BiochemistryFaculty of Medicineand Pistachio Safety Research CenterRafsanjan University of Medical SciencesRafsanjanIran Department of Laboratory SciencesSchool of Paramedical SciencesTorbat Heydariyeh University of Medical SciencesTorbat HeydariyehIran 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2019年第9卷第6期

页      面：240-248页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the RUMS by the grant number 20.1094 

主　　题：Fenugreek seed extract Diosgenin 4-Hydroxy-L-isoleucine Dyslipidemia Obesity 

摘      要：Objective:To investigate anti-dyslipidemic effects of hydroalcoholic fenugreek seed extracts,diosgenin,and 4-OH-Ile on HepG2 cell ***:HepG2 cells were treated with hydroalcoholic fenugreek seed extracts,diosgenin,4-OH-Ile,and ***20 was calculated using the MTT *** cells were then pretreated with IC20 concentrations for 24 and 48 *** time PCR was employed to measure expression of liver X receptor alpha(LXR a),sterol regulatory element-binding protein-1 C(SREBP-1 C),acetyl-CoA carboxylase(ACC),fatty acid synthase(FAS),fibroblast growth factor 21(FGF21),peroxisome proliferator-activated receptor gamma(PPAR γ),and lowdensity lipoprotein receptor(LDLR).Results:The results showed that LXR α(P=0.003,P0.001).,SREBP-1 C(P0.001,P0.001),ACC(P=0.002,P=0.006),and FAS(P0.001,P0.001)were downregulated significantly,while FGF21(P0.001,P0.001),PPAR γ(P=0.004,P0.001),and LDLR(P0.001,P0.001)were upregulated significantly in HepG2 cells treated with the IC20 of hydroalcoholic fenugreek seed extracts,diosgenin,4-OH-Ile,and orlistat in 24 and 48 h,***:Hydroalcoholic fenugreek seed extracts,diosgenin,and 4-OH-Ile significantly modulate the expression of some important lipid metabolism related genes,which is similar to *** foenum.-graecum seed extract or its derivatives should be further investigated for their dyslipidemia effects and its complications.