检索结果-南通市图书馆

Astrocytic effect of low molecular weight heparin-superoxide dismutase conjugate in interleukin-6 overexpressing mice following local cerebral ischemia

引用

Neural Regeneration Research 2009年第2期4卷 148-151页

作者： Yizhao Li Guixiang Cui Qingde Wang Hongxia Liu Xiaoxia Zhang Fengshan Wang Keqin Xie Shandong Provincial Disabled Persons Rehabilitation Center Jinan 250001 Shandong Province China department of Rehabifitation Qilu Hospital of Shandong University Jinan 250012 Shandong Province China department of Neurology Hospital of Shandong Aluminum Company Zibo 255069 Shandong Province China The Third People's Hospital of Heze City Heze 274000 Shandong Province China College of Basic Medical Sciences Shandong University of Traditional Chinese Medicine Jinan 250014 Shandong Province China Biochemical Pharmaceutics Institute School of Pharmaceutical Sciences of Shandong University Jinan 250012 Shandong Province China Toxicology Institute School of Public Health Shandong University Jinan 250012 Shandong Province China

BACKGROUND： Studies have shown that low molecular weight heparin-superoxide dismutase conjugate exhibits a remarkable neuroprotective effect. OBJECTIVE： To investigate the effect of low molecular weight heparin-superoxide dismutase conjugate on astrocytes in an interleukin-6 （IL-6） overexpressing mice following local cerebral ischemia. DESIGN, TIME AND SETTING： Randomized, cytological, controlled, animal study was performed in the department of physiology and Neuroscience, Neurology and Biochemistry and molecular Biology, Medical University of South Carolina from January 2005 to March 2005. MATERIALS： Nine IL-6 transgenic mice, irrespective of gender, were randomly divided into three groups： sham-operated, model, and treatment, with three mice in each group. With exception of the sham-operated group, right middle cerebral artery occlusion was induced in the mice. Expression of glial fibrillary acidic protein, an astrocyte marker, was determined by immunohistochemistry. Low molecular weight heparin-superoxide dismutase conjugate was purchased from Biochemistry and Biotechnique Institute, Shandong University. METHODS： Two minutes prior to ischemia induction, 0.5 mL/kg saline or 20 000 U/kg low molecular weight heparin-superoxide dismutase conjugate were administrated via the femoral artery in the model group and treatment group, respectively. The sham-operated group underwent the same protocols, with the exception of occlusion and treatment. MAIN OUTCOME MEASURES： The number of glial fibrillary acidic protein-positive cells was quantified under light microscopy （x200）. RESULTS： In the sham-operated group, there were a large number of astrocytes in the IL-6 transgenic mice. However, the cell bodies were small, and the branches were few and thin. The number of astrocytes in the model group was remarkably less than the sham-operated group. Compared to the model and sham-operated groups, the number of astrocytes significantly increased, and the cell body becam

关键词： cerebral ischemia astrocytes low molecular weight heparin-superoxide dismutase conjugate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Qu-2,a robust poplar suspension cell line for molecular biology

引用

Journal of Forestry Research 2021年第2期32卷 733-740页

作者： Caixia Liu Kailong Li Meng Wang Erqin Fan Chuanping Yang Junhui Wang Pengyue Fu Xiaolan Ge Heike W.Sederoff Ronald RSederoff Vincent LChiang Sui Wang Guanzheng Qu State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040People’s Republic of China State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of State Forestry AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China department of Plant and Microbial Biology North Carolina State UniversityRaleighNC 27695USA Forest Biotechnology Group Department of Forestry and Environmental ResourcesNorth Carolina State UniversityRaleighNC 27695USA Key Laboratory of Soybean Biology in Chinese Ministry of Education Northeast Agricultural UniversityHarbin 150030People’s Republic of China

Populus *** long been used as model woody plant species for molecular biology ***,tissues of poplar are often recalcitrant to experimental procedures for molecular *** generated a hormone autotrophic poplar suspension cell line from a hybrid of Populus alba×***‘Yinzhong’,named ***-2 cells are suitable as a model biological system for studying woody ***-2 cells have many advantages over suspension cell lines derived so far from any other woody ***-2 cells are very easy to cultivate and can grow on several common plant culture media without the addition of any plant *** show exceptionally high growth rates,reaching an approximately 150-fold increase in biomass after one week of *** important unique characteristic of Qu-2 cells is that they can be cryopreserved and readily ***-2 cells are suitable for molecular manipulations such as protoplast production,transient transformation,and RNA-seq ***,Qu-2 cells have the great potential to be an excellent model cell line in tree molecular biological research,ranging from physiology to gene *** Qu-2 cells will be made available to the plant community for research.

关键词： Qu-2 cell line Suspension cell Poplar Protoplast isolation Transient transformation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

ATP binding cassette proteins ABCG37 and ABCG33 function as potassium-independent cesium uptake carriers in Arabidopsis roots

引用

molecular Plant 2021年第4期14卷 664-678页

作者： Mohammad Arif Ashraf Takashi Akihiro Keita Ito Sayaka Kumagai Ryohei Sugita Keitaro Tanoi Abidur Rahman United Graduate School of Agricultural Sciences Iwate UniversityMoriokaIwate 020-8550Japan Faculty of Lite and Environmental Science Shimane UniversityMatsueShimane 690-8504Japan Faculty of Agriculture Department of Plant Bio SciencesIwate UniversityMoriokaIwate 0208550Japan Graduate School of Agricultural and Life Sciences.The University of Tokyo Tokyo 113-8654.Japan PRESTO Japan Science and Technology Agency(JST)4-1-8 HonchoKawaguchiSaitama 332-0012Japan Agr-Innovation Iwate UniversityMoriokaIwate 020-8550Japan These authors contributed equally Present address:Biology department University of MassachusettsAmherstMA USA

Radiocesium accumulated in the soil by nuclear accidents is a major environmental *** transport process of cesium(Cs^(+))is tightly linked to the indispensable plant nutrient potassium(K^(+))as they both belong to the group I alkali metals with similar chemical *** of the transporters that had been characterized to date as Cs*transporters are directly or indirectly linked to K^(+).Using a combinatorial approach of physiology,genetics,cell biology,and root uptake assay,here we identified two ATP-binding cassette(ABC)proteins,ABCG37 and ABCG33,as facilitators of Cs^(+)influx.A gain-of-function mutant of ABCG37(abcg37-1)showed increased sensitivity to Cs^(+)-induced root growth inhibition,while the double knockout mutant of ABCG33 and ABCG37(abcg33-1abcg37-2)showed resistance,whereas the single loss-of-function mutants of ABCG33 and ABCG37 did not show any alteration in Cs^(+)*** planta short-term radioactive Cs^(+)-uptake assay along with growth and uptake assays in a heterologous system confirmed ABCG33 and ABCG37 as Cs^(+)-uptake *** response and content were unaffected in the double-mutant background and yeast cells lacking potassium-uptake carriers transformed with ABCG33 and ABCG37 failed to grow in the absence of K^(+),confirming that Cs^(+)uptake by ABCG33 and ABCG37 is independent of K^(+).Collectively,this work identified two ABC proteins as new Cs^(+)-influx carriers that act redundantly and independent of the K^(+)-uptake pathway.

关键词： cesium transport ABC proteins uptake Arabidopsis root biology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Drought-responsive genes expressed predominantly in root tissues are enriched with homotypic cis-regulatory clusters in promoters of major cereal crops

引用

The Crop Journal 2017年第3期5卷 195-206页

作者： Muhammad Ramzan Khan Imran Khan Zahra Ibrar Jens Léon Ali Ahmed Naz Institute of Crop Science and Resource Conservation(INRES) Department of Crop Genetics and BiotechnologyRheinische Friedrich-Wilhelms University of BonnGermany National Centre for Bioinformatics(NCB) Quaid-i-Azam UniversityIslamabadPakistan National Institute for Genomics and Advanced Biotechnology(NIGAB) National Agricultural Research CentrePark RoadIslamabadPakistan

The root appears to be the most relevant organ for breeding drought stress ***, our knowledge about temporal and spatial regulation of drought-associated genes in the root remains fragmented, especially in crop plants. We performed a meta-analysis of expression divergence of essential drought-inducible genes and analyzed their association with cis-elements in model crops and major cereal crops. Our analysis of42 selected drought-inducible genes revealed that these are expressed primarily in roots,followed by shoot, leaf, and inflorescence tissues, especially in wheat. Quantitative real-time RT-PCR analysis confirmed higher expression of TaDREB2 and TaAQP7 in roots,correlated with extensive rooting and drought-stress tolerance in wheat. A promoter scan up to 2 kb upstream of the translation start site using phylogenetic footprinting revealed708 transcription factor binding sites, including drought response elements(DREs), auxin response elements(Aux REs), MYCREs/MYBREs, ABAREs, and ERD1 in 19 selected ***, these elements were organized into clusters of overlapping transcription factor binding sites known as homotypic clusters(HCTs), which modulate drought physiology in plants. Taken together, these results revealed the expression preeminence of major drought-inducible genes in the root, suggesting its crucial role in drought adaptation. The occurrence of HCTs in drought-inducible genes highlights the putative evolutionary modifications of crop plants in developing drought adaptation. We propose that these DNA motifs can be used as molecular markers for breeding drought-resilient cultivars, particularly in the cereal crops.

关键词： Gene expression Promoter DREB cis-regulatory elements Phylogeny Drought adaptation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Myorelaxation in the training process of skilled athletes

引用

Open Journal of molecular and Integrative physiology 2013年第2期3卷 83-86页

作者： Yuriy P. Denisenko Yuriy V. Vysochin Leonid G. Yatsenko department of Physical Education St. Petersburg State Technological University of Vegetative Polymers St. Petersburg Russia department of Theory and Methods of Sports Games Branch of Povolzhskaya State Academy of Physical Culture Sports and Tourism Naberezhnye Chelny Russia

At the present time, a number of various ways of sportspersons’ special physical capability (SPC) based mainly on training and competitive loadings ramp up. They are effective enough to reach the main goal, but none of them provides sportspersons’ health safety. Moreover, with the increase in volume and intensity of the loadings, which in sport have almost reached their limits, the sport traumatism and morbidity rate grow progressively. Proceeding from this, there was an evident necessity for the search of conceptually new ways for a simultaneous solution of these two the most complex and, in the opinion of many research workers, almost incompatible problems—the problem of achieving the highest levels of special physical working capacity, and the problem of sportspersons’ health maintenance and improving-associated by us into one general problem of human motor activity efficiency enhancement. Therefore it is necessary to physiologically substantiated the basic methods and principles of special relaxation training, directed on increase of efficiency of process of training of sportsmen at all stages of the development of athletic skills. Under the efficiency of the motor activity, we understand achieve the highest levels of the special physical performance while maintaining sports longevity and health of athletes.

关键词： Extreme Conditions Protection Functional System Muscle Relaxation Rate Physical Efficiency Central Nervous System Relaxation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

青少年应激引起腹侧海马长时程增强的持续增加：星形细胞谷氨酰胺合成酶的作用

引用

神经损伤与功能重建 2019年第11期14卷 F0003-F0003页

作者： Ivens S ?al??kan G Papageorgiou I Cesetti T Malich A Kann O Heinemann U Stork O Albrecht A 杜一星 department of Psychiatry and Psychotherapy Charité-Universit?tsmedizin Berlin Institute for Neurophysiology Charité-Universit?tsmedizin Berlin Institute of Biology Otto-von-Guericke-University Magdeburg Center for Behavioral Brain Sciences Institute of physiology and Pathophysiology University of Heidelberg Institute of Radiology Suedharz Hospital Nordhausen Institute of molecular and Cell Biology University of Applied Sciences Mannheim

童年期创伤是引发与压力有关的精神病的最重要风险因素之一,例如创伤后压力障碍或抑郁。尽管已证实星形胶质细胞在调节递质释放和突触可塑性中发挥作用,然而目前尚不清楚星形胶质递质代谢在这种应激诱导的精神病理学中所起的作用。在啮... 详细信息

童年期创伤是引发与压力有关的精神病的最重要风险因素之一,例如创伤后压力障碍或抑郁。尽管已证实星形胶质细胞在调节递质释放和突触可塑性中发挥作用,然而目前尚不清楚星形胶质递质代谢在这种应激诱导的精神病理学中所起的作用。在啮齿动物,可以通过青少年应激暴露来模拟童年逆境,从而导致焦虑加剧以及成年后应对压力的能力下降。我们描述了这种幼年应激大鼠,无论成年期是否有其他应激,都会导致腹侧CA1(v CA1)Schaffer侧支的突触效力降低,而高频刺激后突触传递的长时程增强(LTP)增加。我们通过阻断星形胶质谷氨酸降解酶谷氨酰胺合成酶(GS)测试谷氨酸-谷氨酰胺循环是否引起青少年应激后可塑性的持久变化。用蛋氨酸亚砜亚胺对正常大鼠的切片中GS的药理抑制的确模仿青少年应激对vCA1-LTP的影响,而提供谷氨酰胺足以使LTP正常化。评估在青少年、成年期或青少年/成年联合应激后,vCA1辐射层中的稳态mRNA水平揭示了GS、星形细胞谷氨酸和谷氨酰胺转运蛋白表达的明显变化。尽管青少年应激后GS mRNA表达水平持续降低,但GS蛋白水平保持稳定。本研究结果表明,星形胶质细胞和谷氨酸-谷氨酰胺循环在介导青少年应激对vCA1(与焦虑和情绪记忆处理有关的区域)的可塑性的长期影响中起关键作用。

关键词：星形胶质细胞谷氨酸-谷氨酰胺循环谷氨酰胺合成酶青少年应激长时程增强腹侧海马

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Notes,outline and divergence times of Basidiomycota

引用

Fungal Diversity 2019年第6期99卷 105-367页

作者： Mao-Qiang He Rui-Lin Zhao Kevin D.Hyde Dominik Begerow Martin Kemler Andrey Yurkov Eric H.C.McKenzie Olivier Raspe Makoto Kakishima Santiago Sanchez-Ramırez Else C.Vellinga Roy Halling Viktor Papp Ivan V.Zmitrovich Bart Buyck Damien Ertz Nalin N.Wijayawardene Bao-Kai Cui Nathan Schoutteten Xin-Zhan Liu Tai-Hui Li Yi-Jian Yao Xin-Yu Zhu An-Qi Liu Guo-Jie Li Ming-Zhe Zhang Zhi-Lin Ling Bin Cao Vladimir Antonin Teun Boekhout Bianca Denise Barbosa da Silva Eske De Crop Cony Decock Balint Dima Arun Kumar Dutta Jack W.Fell Jozsef Geml Masoomeh Ghobad-Nejhad Admir J.Giachini Tatiana B.Gibertoni Sergio P.Gorjon Danny Haelewaters Shuang-Hui He Brendan P.Hodkinson Egon Horak Tamotsu Hoshino Alfredo Justo Young Woon Lim Nelson Menolli Jr Armin Mesic Jean-Marc Moncalvo Gregory M.Mueller La szlo G.Nagy RHenrik Nilsson Machiel Noordeloos Jorinde Nuytinck Takamichi Orihara Cheewangkoon Ratchadawan Mario Rajchenberg Alexandre G.S.Silva-Filho Marcelo Aloisio Sulzbacher Zdenko Tkalcec Ricardo Valenzuela Annemieke Verbeken Alfredo Vizzini Felipe Wartchow Tie-Zheng Wei Michael WeiB Chang-Lin Zhao Paul M.Kirk State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101People’s Republic of China department of Entomology and Plant Pathology Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand College of Life Sciences University of Chinese Academy of SciencesHuairou DistrictBeijing 100408People’s Republic of China Ruhr-Universitat Bochum AG GeobotanikND03UniversitatsstraBe 15044801 BochumGermany Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures InhoffenstraBe 7B38124 BrunswickGermany Manaaki Whenua-Landcare Research Private Bag 92170AucklandNew Zealand Research department Meise Botanic GardenNieuwelaan 381860 MeiseBelgium Service General de l'Enseignement superieur et de la Recherche scientifique Federation Wallonie-BruxellesLavallee 11080 BrusselsBelgium University of Tsukuba TsukubaIbaraki 305-8572Japan department of Ecology and Evolutionary Biology University of Toronto25 Willcocks St.TorontoON M5S 3B2Canada Berkeley USA Institute of Systematic Botany The New York Botanical GardenBronxNY 10458-5126USA department of Botany Szent Istvan University1518 BudapestHungary Komarov Botanical Institute Russian Academy of SciencesProfessor Popov st.2Saint PetersburgRussia 197376 Institut pour Systematique Ecologie Biodiversite(ISYEB UMR 7205) Sorbonne universiteMuseum National d’Histoire Naturelle CP 39CNRSDepartement Origines et Evolution12 Rue Buffon75005 ParisFrance Institute of Microbiology Beijing Forestry UniversityBeijing 100083People’s Republic of China department of Biology Research Group MycologyGhent UniversityK.L.Ledeganckstraat 359000 GhentBelgium State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbial Culture Collection and ApplicationGuangdong Institute of MicrobiologyGuangzhou 510070People’s Republic of China department of Botany Moravian Mu

The Basidiomycota constitutes a major phylum of the kingdom Fungi and is second in species numbers to the *** present work provides an overview of all validly published,currently used basidiomycete genera to date in a single *** outline of all genera of Basidiomycota is provided,which includes 1928 currently used genera names,with 1263 synonyms,which are distributed in 241 families,68 orders,18 classes and four *** provide brief notes for each accepted genus including information on classification,number of accepted species,type species,life mode,habitat,distribution,and sequence ***,three phylogenetic analyses with combined LSU,SSU,5.8s,rpb1,rpb2,and ef1 datasets for the subphyla Agaricomycotina,Pucciniomycotina and Ustilaginomycotina are conducted,*** time estimates are provided to the family level with 632 species from 62 orders,168 families and 605 *** study indicates that the divergence times of the subphyla in Basidiomycota are 406-430 Mya,classes are 211-383 Mya,and orders are 99-323 Mya,which are largely consistent with previous *** this study,all phylogenetically supported families were dated,with the families of Agaricomycotina diverging from 27-178 Mya,Pucciniomycotina from 85-222 Mya,and Ustilaginomycotina from 79-177 *** times as additional criterion in ranking provide additional evidence to resolve taxonomic problems in the Basidiomycota taxonomic system,and also provide a better understanding of their phylogeny and evolution.

关键词： Classification molecular clock Fungi Systematics Taxonomy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Within-litter variation in birth weight: impact of nutritional status in the sow

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第6期16卷 417-435页

作者： Tao-lin YUAN Yu-hua ZHU Meng SHI Tian-tian LI Na LI Guo-yao WU Fuller W.BAZER Jian-jun ZANG Feng-lai WANG Jun-jun WANG State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural University department of Animal Science Texas A&M UniversityCollege Station

Accompanying the beneficial improvement in litter size from genetic selection for high-prolificacy sows, within-litter variation in birth weight has increased with detrimental effects on post-natal growth and survival due to an increase in the proportion of piglets with low birth-weight. Causes of within-litter variation in birth weight include breed characteristics that affect uterine space, ovulation rate, degree of maturation of oocytes, duration of time required for ovulation, interval between ovulation and fertilization, uterine capacity for implantation and placentation, size and efficiency of placental transport of nutrients, communication between conceptus/fetus and maternal systems, as well as nutritional status and environmental influences during gestation. Because these factors contribute to within-litter variation in birth weight, nutritional status of the sow to improve fetal-placental development must focus on the following three important stages in the reproductive cycle： pre-mating or weaning to estrus, early gestation and late gestation. The goal is to increase the homogeneity of development of oocytes and conceptuses, decrease variations in conceptus development during implantation and placentation, and improve birth weights of newborn piglets. Though some progress has been made in nutritional regulation of within-litter variation in the birth weight of piglets, additional studies, with a focus on and insights into molecular mechanisms of reproductive physiology from the aspects of maternal growth and offspring development, as well as their regulation by nutrients provided to the sow, are urgently needed.

关键词： Within-litter variation Pig Mortality Morbidity Growth Sow nutrition

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Join us on an amazing journey towards next-generation treatments for CNS disorders:Launch of Neuroprotection,a new high-quality journal in translational neuroscience

引用

Neuroprotection 2023年第1期1卷 5-8页

作者： Xunming Ji Piotr Walczak Heleen M.M.van Beusekom Ana I.Casas Andrew Clarkson Tracy Farr Jukka Jolkkonen Yajie Liang Michel M.Modo Paulo H.Rosado-de-Castro Karsten Ruscher Yan-Jiang Wang Haitao Wu Marietta Zille Shen Li Johannes Boltze Beijing Institute of Brain Disorders Capital Medical UniversityBeijingChina department of Diagnostic Radiology and Nuclear Medicine Center for Advanced Imaging ResearchUniversity of MarylandBaltimoreMarylandUSA department of Cardiology Erasmus MC RotterdamUniversity Medical Center RotterdamRotterdamThe Netherlands department of Neurology Center for Translational Neuro‐and Behavioral Sciences(C‐TNBS)University Clinics EssenEssenGermany department of Anatomy Brain Health Research Center and Brain Research New ZealandDunedinNew Zealand Medical School Queen's Medical CentreUniversity of NottinghamNottinghamUK A.I.Virtanen Institute for molecular Sciences University of Eastern FinlandKuopioFinland McGowan Institute for Regenerative Medicine University of PittsburghPittsburghPennsylvaniaUSA department of Radiology University of PittsburghPittsburghPennsylvaniaUSA department of Bioengineering University of PittsburghPittsburghPennsylvaniaUSA department of Anatomy and department of Radiology School of MedicineInstitute of Biomedical SciencesFederal University of Rio de JaneiroRio de JaneiroBrazil Laboratory for Experimental Brain Research University of LundLundSweden department of Neurology Daping HospitalCenter for Clinical NeuroscienceChongqingChina department of Neurobiology Beijing Institute of Basic Medical SciencesBeijingChina department of Pharmaceutical Sciences Division of Pharmacology and ToxicologyUniversity of ViennaViennaAustria department of Neurology and Psychiatry Beijing Shijitan HospitalCapital Medical UniversityBeijingChina School of Life Sciences University of WarwickCoventryUK

Translational medicine in neurodegenerative and neurovascular diseases is approaching a breakthrough *** years have led to dramatic progress in both experimental and clinical *** on a much better and continuously increasing understanding of disease mechanisms,progression and pathophysiology,new therapies with an improved translational potential to protect tissue either against acute or chronic degeneration and even approaches potentially capable of repairing damaged brain tissue are emerging.

关键词： cytoprotection nervous system repair neurodegeneration neuroprotection neurovasculardiseases translational research

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of diets supplemented with linseed alone or combined with vitamin E and selenium or with plant extracts,on Longissimus thoracis transcriptome in growing-finishing Italian Large White pigs

引用

Journal of Animal Science and Biotechnology 2019年第1期10卷 159-171页

作者： Rubina Sirri Marika Vitali Paolo Zambonelli Giulia Giannini Martina Zappaterra Domenico Pietro Lo Fiego Dalal Sami Roberta Davoli Interdepartmental Centre for Industrial Agrifood Research (CIRI- AGRO) University of Bologna department of Agricultural and Food Sciences (DISTAL) University of Bologna department of Life Sciences University of Modena and Reggio Emilia Interdepartmental Research Centre for Agri-Food Biological Resources Improvement and Valorisation (BIOGEST-SITEIA) University of Modena and Reggio Emilia

Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat *** composition has been also demonstrated to influence the gene expression with effect on biological processes and ***,the knowledge on the effect of nutrients at the molecular level is *** particular,studies on the effects of antioxidants and polyphenols dietary supplementation have been investigated mainly in rodents,and only scarcely in farm animals so ***-Seq with next-generation sequencing is increasingly the method of choice for studying changes in the transcriptome and it has been recently employed also in pig nutrigenomics studies to identify diet-induced changes in gene *** present study aimed to investigate the effect of diets enriched with functional ingredients(linseed,vitamin E and plant extracts) on the transcriptome of pig Longissimus thoracis to elucidate the role of these compounds in influencing genes involved in muscle physiology and metabolism compared to a standard ***: Eight hundred ninety-three significant differentially expressed genes(DEGs)(FDR adjusted P-value ≤ 0.05)were detected by RNA-Seq analysis in the three diet comparisons(D2-D1,D3-D1,D4-D1).The functional analysis of DEGs showed that the diet enriched with n-3 PUFA from linseed(D2) mostly downregulated genes in pathways and biological processes(BPs) related to muscle development,contraction,and glycogen metabolism compared to the standard *** diet supplemented with linseed and vitamin E/Selenium(D3) showed to mostly downregulate genes linked to oxidative *** few genes involved in extracellular matrix(ECM)organization were upregulated by the ***,the comparison D4-D1 showed that the diet supplemented with linseed and plant extracts(D4) upregulated the majority of genes compared to D1 that were involved in a complex network of pathways and BPs all connected by hub *** particular,IGF2 wa

关键词： Diet supplementation Functional analysis Gene expression Linseed Muscle transcriptome n-3 PUFA Pigs Plant extracts Polyphenols Vitamin E

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：