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Journal of Energy Chemistry 2024年第10期97卷 120-127页

作者： Mahammad Rafi Shaik Yongmin Park Young-Kwang Jung Won Bin Im Division of Materials Science and engineering Hanyang University department of Chemical engineering and biotechnology University of Cambridge

Anode materials for rechargeable electric car batteries are obtained from Li-metal owing to their extremely high specific capacity and low redox ***,safety concerns related to dendrite formation on the anode surface caused by the uneven distribution of Li-ions during the discharge process interfere with the use of Li-metal in industrial *** this study,methyl vinyl sulfone（MVS）,a sulfone-based functional electrolyte additive,is used in an additive engineering strategy to control Lielectrolyte interactions and address the aforementioned *** dendrite growth may be restricted,and transition metal degradation on the surface of the cathode can be reduced by the MVS-derived functional electrolyte additive interfacial *** electrochemical performance of an ethylene carbonate/dimethyl carbonate（EC/DMC）+1 wt% MVS Li-metal anode of a Li||Li symmetric cell exhibits remarkable cycle stability,maintaining a low overvoltage for over 750 h at 1 mA cm-2,and capacity of 1 mA h ***,LiNi0.8Co0.1Mn0.1O2（NCM811） full cells with the MVS additive exhibit enhanced electrochemical stability for 250 cycles at a current density of 100 mA *** study provides an innovative approach for stabilizing the metal-electrolyte interfacial layer that may be used for practical applications in metal-based rechargeable batteries.

关键词： Bifunctional electrolyte Dendrite-free Electrolyte additive Interfacial layer Lithium rechargeable battery

Enhanced extracellular production of alpha-lactalbumin from Bacillus subtilis through signal peptide and promoter screening

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food Science and Human Wellness 2024年第4期13卷 2310-2316页

作者： Yuqi Zhu Pengdong Sun Chunjian Li Yu Zhang Yu Wang Jingyuan Li Yanfeng Liu Jian Chen Yang Deng College of food Science and engineering Qingdao Agricultural UniversityQingdao 266109China Key Laboratory of Special food Processing(Co-construction by Ministry and Province) Ministry of Agriculture Rural AffairsQingdao Agricultural UniversityQingdao 266109China Qingdao Special food Research Institute Qingdao 266109China Science Center for Future foods Engineering Research Center of Ministry of Education on Food Synthetic BiotechnologyJiangsu Province Engineering Research Center of Food Synthetic BiotechnologyJiangnan UniversityWuxi 214122China Key Laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Alpha-lactalbumin(α-LA)is a major whey protein found in breast milk and plays a crucial role in the growth and development of *** this study,Bacillus subtilis RIK1285 harboring AprE signal peptide(SP)was selected as the original strain for the production ofα-*** was found thatα-LA was identified in the pellet after ultrasonic disruption and centrifugation instead of in the fermentation *** original strain most likely only producedα-LA intracellular,but not *** improve the expression and secretion ofα-LA in RIK1285,a library of 173 homologous SPs from the *** 168 genome was fused with target LALBA gene in the pBE-S vector and expressed extracellularly in *** YjcN was determined to be the best signal *** in supernatant were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and purified by nickel column to calculate the highest yield signal *** addition,different promoters(P_(aprE),P_(43),and P_(glv))were compared and *** results indicated that the strain RIK1285-pBE-P_(glv)-YjcN-LALBA had the highestα-LA yield,reaching 122.04μg/*** study demonstrates successful expression and secretion of humanα-LA in *** and establishes a foundation for simulating breast milk for infant formulas and developing bioengineered milk.

关键词： Bacillus subtilis Alpha-lactalbumin Bioengineering milk Signal peptide Promoter screening

Constructing a biofunctionalized 3D-printed gelatin/sodium alginate/chitosan tri-polymer complex scaffold with improvised biological andmechanical properties for bone-tissue engineering

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Bio-Design and Manufacturing 2024年第1期7卷 57-73页

作者： Amit Kumar Singh Krishna Pramanik Amit Biswas Centre of Excellence in Tissue engineering Department of Biotechnology and Medical EngineeringNational Institute of TechnologyRourkela 769008India

Sodium alginate(SA)/chitosan(CH)polyelectrolyte scaffold is a suitable substrate for tissue-engineering *** present study deals with further improvement in the tensile strength and biological properties of this type of scaffold to make it a potential template for bone-tissue *** experimented with adding 0%–15%(volume fraction)gelatin(GE),a protein-based biopolymer known to promote cell adhesion,proliferation,and *** resulting tri-polymer complex was used as bioink to fabricate SA/CH/GEmatrices by three-dimensional(3D)*** studies using scanning electron microscopy revealed the microfibrous porous architecture of all the structures,which had a pore size range of 383–419μm.X-ray diffraction and Fourier-transform infrared spectroscopy analyses revealed the amorphous nature of the scaffold and the strong electrostatic interactions among the functional groups of the polymers,thereby forming polyelectrolyte complexes which were found to improve mechanical properties and structural *** scaffolds exhibited a desirable degradation rate,controlled swelling,and hydrophilic characteristics which are favorable for bone-tissue *** tensile strength improved from(386±15)to(693±15)kPa due to the increased stiffness of SA/CH scaffolds upon addition of *** enhanced protein adsorption and in vitro bioactivity(forming an apatite layer)confirmed the ability of the SA/CH/GE scaffold to offer higher cellular adhesion and a bone-like environment to cells during the process of tissue *** vitro biological evaluation including the MTT assay,confocal microscopy analysis,and alizarin red S assay showed a significant increase in cell attachment,cell viability,and cell proliferation,which further improved biomineralization over the scaffold *** addition,SA/CH containing 15%gelatin designated as SA/CH/GE15 showed superior performance to the other fabricated 3D structures,demonstrating its pote

关键词： Scaffold Biomaterial Sodium alginate Chitosan Gelatin 3D printing Tissue engineering

Fatty acids derived from oviposition systems guide female black soldier flies(Hermetia illucens)toward egg deposition sites

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Insect Science 2024年第4期31卷 1231-1248页

作者： Patrick Klüber Emna Arous Jeanny Jerschow Marco Fraatz Daniel Bakonyi Martin Rühl Holger Zorn department Bioresources Fraunhofer Institute for Molecular Biology and Applied EcologyGiessenGermany Institute of food Chemistry and food biotechnology Justus Liebig University GiessenGiessenGermany

The black soldier fly,Hermetia illucens,comes with big promises for industrial purposes since its larvae feed polyphagously on a broad spectrum of organic ***,research focusing on adult flies is scarce,which is inconsistent with their reproductive relevance within the rearing *** particular,directed oviposition is a challenge in artificial ***,decomposing organic matter is commonly used as oviposition substrate,which has extensive potential for improvement in view of the lack of standardization and the risk of microbial ***,we identified three fatty acids and one fatty acid methyl ester derived from the surface of old oviposition sites and targeted to elucidate their effect on preference behavior and oviposition site selection using Y-olfactometry and prepared oviposition sites,*** to tetradecanoic acid attracted gravid females and stimulated oviposition most strongly,while decanoic acid demonstrated a repulsive *** kept in mixed-sex populations were attracted by tetradecanoic acid,resulting in a higher egg mass found in the compound box(3.0–11.4 fold),a≥2.3 fold reduction of nonspecifically deposited eggs,and the highest total egg ***,decanoic and dodecanoic acid caused females to lay a greater proportion of eggs nonspecifically outside both *** data suggest that fatty acids,especially tetradecanoic acid,are important cues for oviposition site selection in black soldier *** order to achieve a directed oviposition behavior,the role of further short-and long-chain fatty acids as attractants should be examined.

关键词： black soldier fly Hermetia illucens fatty acids insects as food and feed oviposition oviposition site selection tetradecanoic acid

Phenolic Profile and Volatiles of in vitro Propagated Lavandula angustifolia ***

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 427-444页

作者： Mir Abdullatif Yahya Fatma Tunali Dilek Killi Atalay Sökmen biotechnology department Konya Food and Agriculture UniversityKonya42080Türkiye Plant Production and Technologies department Konya Food and Agriculture UniversityKonya42080Türkiye

An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller,a medicinal aromatic plant that is a prominent source of volatile organic compounds(VOCs).Murashige and Skoog media were supplemented with various concentrations of Plant Growth Regulators(PGRs),and the growth parameters of the nodal segments were *** explants formed callus when they were supplemented with 2 mg/L of 6-Benzylaminopurine(BAP).The superior hormonal concentration of Murashige and Skoog(MS)media for the proliferation of shoots from callus cultures(39.33%)was 5 mg/L of 2-Isopentenyl adenine(2iP),and the favorable media for the growth of *** callus cultures was 1 and 2 mg/L of BAP,with a 98%for-mation rate in each *** callus cultures and in vitro propagated *** seedlings obtained from var-ious PGR concentrations of MS media were exposed to qualitative and quantitative analysis in terms of phenolic proﬁles,ﬂavonoids,High-performance liquid chromatography(HPLC)analysis of phenolic acids,and headspace-SPME analysis for *** analysis revealed that micropropagated seedlings grown in media containing 1 mg/L of 6-Furfurylaminopurine(KIN)accumulated the highest yield(11.95±0.01 mg GAE/g)of phenolic *** contrast,the lowest concentration(2.17±0.04 mg GAE/g)was detected in 0.5 mg/L of BAP+0.5 mg/L of Naphthaleneacetic acid(NAA)*** plantlets grown in 0.5 mg/L of BAP+0.5 mg/L of NAA media showed the highestﬂavonoid yield(31.67±0.06μg/g QE/g).In contrast,callus samples exhibited the lowest yield(11.59±0.02μg/g QE/g)ofﬂavonoids in MS media supplemented with a concentration of 0.5 mg/L of *** analysis revealed the variability of phenolic acid contents within the callus cultures as well as plantlets,with gallic acid,4-OH benzoic acid,chlorogenic acid,vanillic acid,caffeic acid,cinnamic acid,and rosmarinic acid being the prominent *** presence of twenty-two chemicals was revealed by headspace-SPME ***

关键词： Lavender indirect organogenesis micropropagation This

Lactobacillus plantarum CCFM1180 attenuates obesity induced by estrogen deficiency by activating estrogen receptor alpha in abdominal adipose tissue and regulating gut microbiota-derived metabolites

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food Science and Human Wellness 2024年第2期13卷 765-777页

作者： Qian Chen Chunxia Mei Min Guo Botao Wang Haiqin Chen Jianxin Zhao Gang Wang Wei Chen State Key Laboratory of food Science and Resources Jiangnan UniversityWuxi 214122China School of food Science and Technology Jiangnan UniversityWuxi 214122China National engineering Research Center for Functional food Jiangnan UniversityWuxi 214122China (Yangzhou)Institute of food biotechnology Jiangnan UniversityYangzhou 225004China Bloomage biotechnology Co. Ltd.Jinan 250000China

Lipid metabolism disorders commonly occur during *** deficiency has been shown to lead to excessive energy intake and abnormal lipid metabolism in ovariectomized rats,resulting in *** exhibit anti-obesity properties,and their underlying mechanism has been widely *** this study,we demonstrated the metabolic benefits of Lactobacillus plantarum CCFM1180 in suppressing appetite,controlling body weight,correcting obesity-induced abnormalities,enhancing liver lipid metabolism,and protecting liver function in estrogen-deficient *** mechanisms associated with the anti-obesity and anti-dyslipidemia effects of CCFM1180 on estrogen-deficient rats were *** results showed that CCFM1180 dramatically reduced food intake by activating the expression of estrogen receptor alpha(ERα)and increasing the level of leptin in abdominal adipose *** changes,combined with the increased butyrate concentration and recovered bile acid structure,helped enhance lipid ***,CCFM1180 treatment was found to be safer than exogenous estrogen ***,*** CCFM1180 could be considered a new therapeutic strategy for preventing and alleviating menopausal lipid abnormalities.

关键词： Bile acid Gut microbiota Menopause Ovariectomy Short chain fatty acid

Combined Insights from Leachate Structure and Microstructure Characteristics for Eating Quality of Convenience Rice Processed by Super-Heated and Pressurized Steam Technologies

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Rice science 2024年第4期31卷 475-488,I0035,I0036页

作者： Mingyo HA Hyo-Young JEONG Ju Hun LEE Hyun-Jung CHUNG Division of food and Nutrition Chonnam National UniversityGwangju 61186South Korea department of biotechnology College of Life Sciences and BiotechnologyKorea UniversitySeoul 02841South Korea Division of food Science&Culinary Arts Shinhan UniversityGyeonggi 11644South Korea

Convenience rice has become widely popular due to its easy availability for cooking. This study investigated the starch structure and composition of leachate and the microstructure of reheated convenience rice using novel processing technologies: super-heated steaming(SHS), auto-electric cooking(AEC), and pressurized-steam cooking(PSC). Additionally, the effect of two different target water contents(58% and 63%) was also evaluated. The PSC_63% sample had the highest total solids and amylopectin amount in the leachate. The amylopectin amount in the leachate differed significantly based on the targeted water content. Morphological characterization revealed that the swelling of starch and the coated layer on the surface of rice grains were most pronounced in the PSC_63% sample due to the pressure processing. The textural hardness of the AEC_58% sample was much higher than that of the other samples. The PSC_63% sample had the highest textural adhesiveness value, which can be attributed to the highest amylopectin amount in the leachate. Sensory characterization showed that the PSC_63% sample had the highest glossiness, whiteness, moistness, and overall acceptability. The principal component analysis score plots presented substantial differences in the leachate and textural and sensory characteristics of reheated convenience rice among the different processing technologies.

关键词： reheated convenience rice starch structure leachate multi-scale structure eating quality processing technology

Technological advancements in millet dehulling and polishing process: An insight into pretreatment methods, machineries and impact on nutritional quality

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Grain & Oil Science and Technology 2024年第3期7卷 186-195页

作者： Shagolshem Mukta Singh Jayasree Joshi T P.Srinivasa Rao Agricultural and food engineering department Indian Institute of Technology Kharagpur

Millets are widely recognized for their nutritional significance; however, the methods employed for their processing are currently lacking. This article primarily focuses on the advanced technologies and progressions in millet dehulling and polishing. These technologies operate based on the fundamental principles of compression-shearing, abrasion-friction, and centrifugal-impact forces. Processing of millets can be challenging because of the physical characteristics and tight attachment of hull and bran to the endosperm. However, several dehullers have been designed to solve this problem for different kinds of millets. In addition, the nutritional and anti-nutritional characteristics undergo alterations due to both dehulling and polishing processes. These alterations are thoroughly examined and discussed in this article. Specifically, anti-nutrients such as tannins and phytate are predominantly found in the outer pericarp of the grain and experience a reduction after undergoing dehulling and polishing. The nutritional properties are also subjected to a reduction; however, this reduction can be mitigated by subjecting the grains to certain pretreatments before dehulling and polishing. These treatments serve to enhance dehulling efficiency and nutrient digestibility while simultaneously reducing the presence of anti-nutrients. Novel thermal and non-thermal methodologies such as microwave, hydrothermal, high-pressure processing, and ohmic heating can be employed for processing millets, thereby diminishing the loss of nutrients. Additional research can be carried out to investigate their impact on the dehulling and polishing of millets.

关键词： Dehulling Millets Novel thermal and non-thermal techniques Polishing Pretreatment

Environmental enrichment in combination with Bifidobacterium breve HNXY26M4 intervention amplifies neuroprotective benefits in a mouse model of Alzheimer's disease by modulating glutamine metabolism of the gut microbiome

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food Science and Human Wellness 2024年第2期13卷 982-992页

作者： Guangsu Zhu Min Guo Jianxin Zhao Hao Zhang Gang Wang Wei Chen State Key Laboratory of food Science and Resources Jiangnan UniversityWuxi 214122China School of food Science and Technology Jiangnan UniversityWuxi 214122China (Yangzhou)Institute of food biotechnology Jiangnan UniversityYangzhou 225004China National engineering Center of Functional food Jiangnan UniversityWuxi 214122China

The gut microbiota-brain axis has emerged as a novel target for Alzheimer's disease(AD),a neurodegenerative disease characterised by behavioural and cognitive ***,most previous microbiome-based intervention studies have focused on single factors and yielded only modest cognitive ***,we proposed a multidomain intervention strategy that combined Bifidobacterium breve treatment with environmental enrichment(EE)*** this study,we found that compared with EE or *** treatment alone,*** intervention combined with EE amplified its neuroprotective effects on AD mice,as reflected by improved cognition,inhibited neuroinflammation and enhanced synaptic ***,using microbiome and metabolome profiling,we found that the combination of *** and EE treatment restored AD-related gut microbiota dysbiosis and reversed microbial metabolite ***,by integrating behavioural and neurological data with metabolomic profiles,we revealed that the underlying mechanism may involve the modulation of microbiota-derived glutamine metabolism via gut-brain ***,combined *** intervention with EE treatment can alleviate AD-related cognitive impairment and improve brain function by regulating glutamine metabolism of the gut *** findings provide a promising multidomain intervention strategy,with a combination of dietary microbiome-based and lifestyle-targeted interventions,to promote brain function and delay the progression of AD.

关键词： Alzheimer’s disease Bifidobacterium breve Environmental enrichment Glutamine metabolism Microbiota-gut-brain axis

The modulatory effect of oolong tea polyphenols on intestinal flora and hypothalamus gene expression in a circadian rhythm disturbance mouse model

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food Science and Human Wellness 2024年第2期13卷 748-764页

作者： Ruonan Yan Chi-Tang Ho Yanan Liu Shengnan Zhan Zufang Wu Xin Zhang department of food Science and engineering Ningbo UniversityNingbo 315211China department of food Science Rutgers UniversityNew BrunswickNew Jersey 08901USA

The interaction between host circadian rhythm and gut microbes through the gut-brain axis provides new clues for tea polyphenols to improve host *** present research showed that oolong tea polyphenols(OTP)improved the structural disorder of the intestinal flora caused by continuous darkness,thereby modulating the production of metabolites related to pyruvate metabolism,glycolysis/gluconeogenesis,and tryptophan metabolism to alleviate the steady-state *** fecal microbiota transplantation from the OTP group,the single-cell transcriptomic analysis revealed that OTP significantly increased the number of hypothalamus cell clusters,up-regulated the number of astrocytes and fibroblasts,and enhanced the expression of circadian rhythm genes Cry2,Per3,Bhlhe41,Nr1d1,Nr1d2,Dbp and Rorb in hypothalamic *** results confirmed that OTP can actively improve the intestinal environmental state as well as internal/peripheral circadian rhythm disorders and cognitive impairment,with potential prebiotic functional characteristics to notably contribute to host health.

关键词： Gut microbiota Circadian rhythm Oolong tea polyphenols Single-cell sequencing Metabolism