检索结果-南通市图书馆

Visceral leishmaniasis: An immunological viewpoint on asymptomatic infections and post kala azar dermal leishmaniasis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2018年第2期11卷 98-108页

作者： Neeraj Tiwari Dhiraj Kishore Surabhi Bajpai Rakesh K.Singh department of Biochemistry Institute of ScienceBanaras Hindu University department of Medicine Institute of Medical SciencesBanaras Hindu University department of bioscience and biotechnology Banasthali University

Elimination of visceral leishmaniasis is a priority programme in Indian *** World Health Organization has set a new target to eliminate kala-azar by the year 2020 as previous target elimination year(2015) has *** elimination programme has successfully curbed the rate of infection in endemic regions; however, there are still few challenges in its *** current drug control regime is extremely limited and comprises only two(amphotericin B and miltefosine) drugs, which are also susceptible for parasites ***, these drugs do not produce sterile cure, and cured patients may develop post kala-azar dermal leishmaniasis even after a decade of cure leaving behind a potent source of parasitic reservoirs for further disease transmission.A significant proportion of endemic population remain seropositive but aymptomatic for many years without any clinical symptom that serve as latent parasitic *** lack of tools to identify live parasites in asymptomatic infections and there association in disease transmission, parameters of sterile cure along with post kala-azar dermal leishmaniasis progression remain a major threat in its *** this review, we discuss the potential of host immune inhibitory mechanisms to identify immune correlates of protective immunity to understand the mystery of asymptomatic infections, sterile cure and post kala azar dermal leishmaniasis.

关键词： Visceral leishmaniasis Immune response Asymptomatic infection Sterile cure Post kala-azar dermal leishmaniasis

Complementation and Expression Analysis of SoRablA and SoRab2A in Sugarcane Demonstrates Their Functional Diversification

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2006年第12期48卷 1450-1457页

作者： Jia-Ming Zhang Anne W Sylvester Ding-Qin Li Xue-Piao Sun State Key biotechnology Laboratory for Tropical Crops Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China department of Botany University of Wyoming Laramie Wyoming 82071 USA

Mammalian and plant Rabl and Rab2 are small GTPases that regulate vesicle trafficking in the endoplasmic reticulum （ER） to Golgi compartments. Little is known about their functional diversification or potential interaction. We cloned sugarcane （Saccharum officinarum L.） Rab1A and Rab2A genes and studied their functional differences by expression and complementation experiments. We found differential expression of the two genes during sugarcane leaf development： SoRab2A expression declined from the dividing base to the maturing tip of the growing leaves, whereas SoRab1A was constitutively expressed, suggesting that SoRab2A is required for cell division and expansion and SoRablA is required for cells at all developmental stages. We used a yeast temperature sensitive ypt1-A 136D mutant strain to further investigate these shared and unique functions. Yptl is a small GTPase that regulates vesicle transport in the same cellular location as Rabl and Rab2. Neither SoRab1A nor SoRab2A alone could restore the growth of the mutant at restrictive temperatures when SoRab1A and SoRab2A were transformed separately. However, SoRab1A transformants maintained normal morphology and viability at non-permissive temperature, and resumed growth when returned to permissive temperature, whereas SoRab2A transformants died at non-permissive temperature, suggesting that SoRablA function is required for a cell＇s viability. Mutant growth was fully restored when SoRab1A and SoRab2A were co-transformed, indicating that SoRablA and SoRab2A complement each other and they both are needed to restore the function of ypt1-A136D. These results demonstrate that SoRab1A and SoRab2A serve distinct but overlapping functions, mostly by regulating the transportation of different sets of proteins.

关键词： complementation co-transformation functional diversification Rab GTPase sugarcane temperature sensitivemutant

RNAi of the N-glycosylation-related genes confirms their importance in insect development and α-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2022年第1期29卷 91-99页

作者： Qun Yang Kristof De Schutter Pengyu Chen Els J.M.Van Damme Guy Smagghe Laboratory of Agrozoology Department of Plants and CropsFaculty of Bioscience EngineeringGhent UniversityCoupure links 653Ghent9000Belgium Laboratory of Biochemistry and Glycobiology Department of BiotechnologyFaculty of Bioscience EngineeringGhent UniversityCoupure links 653Ghent9000Belgium

Recently N-glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N-glycosylation in the development of hemimetabolous insects is unknown. To further elucidate the role of N-glycosylation in the development of insects, a functional characterization of the N-glycosylation-related genes (NGRGs) was performed in a model insect for hemimetabolous development, namely the brown planthopper Nilaparvata lugens. In this project, we report the effects of RNAi-mediated silencing of 15 NGRGs on the postembryonic development of N. lugens. Two major observations were made. First, interruption of the early steps of N-glycan processing led to a lethal phenotype during the transition from nymph to adult as was observed in T. castaneum. Second, we report here on an essential function for the α-1,6-fucosyl transferase in the ecdysis event of N. lugens between nymphal instars, since gene-silencing by RNAi led to failure of ecdysis and subsequent mortality of the treated insect.

关键词： development glycosylation Nilaparvata lugens N-glycan postembryonic RNA interference

Recombinant expressed vasoactive intestinal peptide analogue ameliorates TNBS-induced colitis in rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2018年第6期24卷 706-715页

作者： Chun-lan Xu Yu Guo lei Qiao li Ma Yi-Yi Cheng The Key Laboratory for Space bioscience and biotechnology School of Life Sciences Northwestern Polytechnical University

AIM To investigate the modulatory effect of recombinantexpressed vasoactive intestinal peptide(VIP) analogue(rVIPa) on trinitrobenzene sulfonic acid(TNBS)-induced colitis in rats. METHODS Forty-eight rats were randomized into six groups: normal control group(Control), model control group(TNBS), ethanol treatment group(ETOH), and VIP treatment groups with different dosage(rVIPa_(1nmol), rVIPa_(2nmol), rVIPa_(4nmol)). Diarrhea and bloody stool were observed. Colonic damage was evaluated histologically. The levels of tumor necrosis factor-α( TNF-α), interleukin-10(Il-10), myeloperoxidase(MPO) and endotoxin in colonic tissue and serum were determined by enzyme-linked immunosorbent assay(ElISA). The expression of occludin, ZO-1, Toll-like receptor 4(TlR4),and nuclear factor-kappa B p65(NF-κBp65), IκBα, and p-IκBα were detected by Western blot. RESULTS Administration with 2 nmol rVIPa prevented TNBSinduced necrosis, hyperemia, swelling, inflammation, etc., pathologic changes observed in the inner surface of colon in experimental rats. Moreover, rVIPa significantly decreased colonic TNF-α level(P < 0.001), MPO activity(P < 0.001) and serum endotoxin level(P < 0.01), and remarkably increased colonic Il-10 content(P < 0.001) in rats with TNBS-induced colitis. Furthermore, compared to the TNBS-induced colitis group, 2 nmol rVIPa treatment up-regulated the levels of occludin(P < 0.05) and ZO-1(P < 0.05), NF-κB p65(P < 0.01) and IκBα(P < 0.001), and down-regulated the levels of TlR4. CONCLUSION rVIPa ameliorates TNBS-induced colonic injury and inflammation and effectively protected the intestinal mucosal barrier function in rats. The mechanism may be related to TlR4/NF-κB-mediated signaling pathway. rVIPa could be used as a new alternative therapy for intestinal inflammatory disorders.

关键词： Vasoactive intestinal peptide Intestinal mucosal barrier Tight junction Toll-like receptors Recombinant expression

Secretive expression of Aspergillus fumigatus phytase in tobacco improves phosphorus nutrition in plant

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

High Technology Letters 2007年第4期13卷 436-440页

作者：王严 Gao Xiaorong Su Qiao An Lijia department of bioscience and biotechnology School of Environment and Life Dalian University of Technology Dalian 116024 P.R. China

To generate transgenic plants capable of utilizing exogenous phytate, an Aspersgillus fumigatus phytase gene （fphyA） was constitutively expressed in tobacco and recombinant enzyme was secreted from plant roots into the rhizosphere using the signal sequence from tobacco calreticulin. After 40 days of plant growth in hydroponic media, phytase activities in leaves, stems, roots and growth media of transgenic plants were 8.6-fold, 7.4-fold, 12.6-fold and 14.3-fold higher than those of wild-type plants. Signifi- cant improvements in plant growth and phosphorus （P） utilization were observed in the transgenic plants. When phytate was supplied as the sole P source, 45-day-old transgenic tobaccos accumulated 1.0-fold and 0.5-fold more shoot and root biomass than wild-type tobaccos, with a concomitant of l. 7-fold increase in total P concentration. These results indicate that secretive expression of the A. fumigatus phytase improves acquisition and use of P from phytate in plants.

关键词： Aspergillus fumigatus phosphorus phytate phytase gene tobacco

KoNA:Korean Nucleotide Archive as A New Data Repository for Nucleotide Sequence Data

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2024年第1期22卷 161-167页

作者： Gunhwan Ko Jae Ho Lee Young Mi Sim Wangho Song Byung-Ha Yoon Iksu Byeon Bang Hyuck Lee Sang-Ok Kim Jinhyuk Choi Insoo Jang Hyerin Kim Jin Ok Yang Kiwon Jang Sora Kim Jong-Hwan Kim Jongbum Jeon Jaeeun Jung Seungwoo Hwang Ji-Hwan Park Pan-Gyu Kim Seon-Young Kim Byungwook Lee Korea Bioinformation Center Korea Research Institute of Bioscience&BiotechnologyDaejeon 34141Republic of Korea

During the last decade,the generation and accumulation of petabase-scale high-throughput sequencing data have resulted in great challenges,including access to human data,as well as transfer,storage,and sharing of enormous amounts of *** promote data-driven biological research,the Korean government announced that all biological data generated from government-funded research projects should be deposited at the Korea BioData Station(K-BDS),which consists of multiple databases for individual data ***,we introduce the Korean Nucleotide Archive(KoNA),a repository of nucleotide sequence *** of July 2022,the Korean Read Archive in KoNA has collected over 477 TB of raw next-generation sequencing data from national genome *** ensure data quality and prepare for international alignment,a standard operating procedure was adopted,which is similar to that of the International Nucleotide Sequence Database *** standard operating procedure includes quality control processes for submitted data and metadata using an automated pipeline,followed by manual *** ensure fast and stable data transfer,a high-speed transmission system called GBox is used in ***,the data uploaded to or downloaded from KoNA through GBox can be readily processed using a cloud computing service called *** seamless coupling of KoNA,GBox,and Bio-Express enhances the data experience,including submission,access,and analysis of raw nucleotide *** not only satisfies the unmet needs for a national sequence repository in Korea but also provides datasets to researchers globally and contributes to advances in *** KoNA is available at https://***/kona/.

关键词： Korea BioData Station Nucleotide sequence Next-generation sequencing repository Genomics Deposition and access of big data

Protective effect of glutamine on intestinal injury and bacterial community in rats exposed to hypobaric hypoxia environment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2014年第16期20卷 4662-4674页

作者： Chun-Lan Xu Rui Sun Xiang-Jin Qiao Cui-Cui Xu Xiao-Ya Shang Wei-Ning Niu The Key Laboratory for Space bioscience and biotechnology School of Life SciencesNorthwestern Polytechnical University

AIM: To investigate the protective effect of glutamine (Gln) on intestinal injury and the bacterial community in rats exposed to hypobaric hypoxia environment.

关键词： Hypobaric hypoxia Glutamine Intestinal mucosa Immunomodulation Bacterial community

Science Letters:Optimization of soybean (Glycine max (L.) Merrill) in planta ovary transformation using a linear minimal gus gene cassette

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2009年第12期10卷 870-876页

作者： Ming LIU Jun YANG Yun-qing CHENG Li-jia AN department of bioscience and biotechnology Dalian University of Technology Dalian 116024 China Institute of Eco-environmental Sciences Jilin Normal University Siping 136000 China

Soybean transformation by ovary-drip was improved by optimizing the length of the transformation pathway by cutting the styles. These modifications facilitated soybean transformation manipulation and improved transformation reproducibility and efficiency. Using a linear minimal gus gene cassette as the foreign DNA, a maximum transformation frequency of 11% was obtained in flowers of the soybean cultivar ‘Liaodou 14’ with their styles mostly removed, whereas removal of only the stigma, partial style cutting and partial ovary cutting gave transformation frequencies of 0%, 1%, and 2%, respectively. An average transformation frequency of 8.2% was obtained when 619 flowers from three soybean cultivars (‘Liaodou 14’, ‘Liaodou 13’, and ‘Tiefeng 29’) were transformed by this optimized method. Southern blotting analysis showed that the gus reporter gene (encoding β-glucuronidase) was stably inherited with a simple pattern. Reverse transcription-polymerase chain reaction (RT-PCR) and GUS staining confirmed the expression of the gus gene in transgenic plants.

关键词： Soybean Glycine max (L.) Merrill In planta ovary-drip transformation Optimization Style cutting Minimal gus renorter gene cassette

Desulfurization of dibenzothiophene(DBT) by a novel strain Lysinibacillus sphaericus DMT-7 isolated from diesel contaminated soil

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2011年第6期23卷 975-982页

作者： Ashutosh Bahuguna Madhuri K.Lily Ashok Munjal Ravindra N.Singh Koushalya Dangwal department of biotechnology Modern Institute of Technology(MIT)DhalwalaRishikesh 249201UttarakhandIndia department of bioscience and biotechnology Banasthali UniversityBanasthali 304022RajasthanIndia department of Biochemistry S.B.S.P.G.Institute of Biomedical Sciences and ResearchBalawalaDehradun 248001UttarakhandIndia

A new bacterial strain DMT-7 capable of selectively desulfurizing dibenzothiophene（DBT） was isolated from diesel contaminated *** DMT-7 was characterized and identified as Lysinibacillus sphaericus DMT-7（NCBI GenBank Accession ***496620） using 16S rDNA gene sequence *** desulfurized product of DBT,2-hydroxybiphenyl（2HBP）,was identified and confirmed by high performance liquid chromatography analysis and gas chromatography-mass spectroscopy analysis *** desulfurization kinetics revealed that DMT-7 started desulfurization of DBT into 2HBP after the lag phase of 24 hr,exponentially increasing the accumulation of 2HBP up to 15 days leading to approximately 60% desulfurization of the ***,further growth resulted into DBT *** induced culture of DMT-7 showed shorter lag phase of 6 hr and early onset of stationary phase within 10 days for desulfurization as compared to that of non-induced culture clearly indicating the inducibility of the desulfurization pathway of *** addition,Lysinibacillus sphaericus DMT-7 also possess the ability to utilize broad range of substrates as sole source of sulfur such as benzothiophene,3,4-benzo DBT,4,6-dimethyl DBT,and 4,6-dibutyl ***,Lysinibacillus sphaericus DMT-7 could serve as model system for efficient biodesulfurization of diesel and petrol.

关键词： biodesulfurization colony forming units .dibenzothiophene high performance liquid chromatography Lysinibacillus sphaericus DMT-7