RNAi of the N-glycosylation-related genes confirms their importance in insect development and α-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens
作者机构：Laboratory of AgrozoologyDepartment of Plants and CropsFaculty of Bioscience EngineeringGhent UniversityCoupure links 653Ghent9000Belgium Laboratory of Biochemistry and GlycobiologyDepartment of BiotechnologyFaculty of Bioscience EngineeringGhent UniversityCoupure links 653Ghent9000Belgium
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2022年第29卷第1期
页 面：91-99页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
主 题：development glycosylation Nilaparvata lugens N-glycan postembryonic RNA interference
摘 要：Recently N-glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N-glycosylation in the development of hemimetabolous insects is unknown. To further elucidate the role of N-glycosylation in the development of insects, a functional characterization of the N-glycosylation-related genes (NGRGs) was performed in a model insect for hemimetabolous development, namely the brown planthopper Nilaparvata lugens. In this project, we report the effects of RNAi-mediated silencing of 15 NGRGs on the postembryonic development of N. lugens. Two major observations were made. First, interruption of the early steps of N-glycan processing led to a lethal phenotype during the transition from nymph to adult as was observed in T. castaneum. Second, we report here on an essential function for the α-1,6-fucosyl transferase in the ecdysis event of N. lugens between nymphal instars, since gene-silencing by RNAi led to failure of ecdysis and subsequent mortality of the treated insect.
维普期刊数据库