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Food Bioscience 2023年第3期53卷 2516-2524页

作者： Do Hyun Kim Chang Hee Jeong Seo Gu Han Hyun Su Jung Sung Gu Han department of Food Science and Biotechnology of Animal Resources Konkuk UniversitySeoul05029Republic of Korea department of bioresource industrialization Honam National Institute of Biological Resource(HNIBR)Mokpo58762Republic of Korea

Yogurt or fermented milk(FM)stimulates the production of mucin in the colon epithelium,which helps protect against *** is a natural sweetener containing functional compound,*** the present study,we aimed to investigate the effect of stevia leaves extract(ST)on FM and the health effect of ST-added FM on human colon ***,we investigated the impact of the extracts from stevia leaves on the fermentation and quality characteristics of *** addition,we assessed the impact of ST-supplemented FM on mucin production and its anti-inflammatory properties against Escherichia coli O157:H7 in human colon epithelial cells(HT-29/MTX).The FM was enriched with ST(0-0.5%w/v)and fermented using a mixture of lactic acid bacteria(LAB).A control FM supplemented with 0.5%*** results demonstrated that ST significantly increased the fermentation rate of FM by promoting the growth of *** supplementation resulted in an increase in the titratable acidity,water-holding capacity,total solid content,and viscosity of *** of HT-29/MTX cells with STFM supernatants led to higher mucus production,as evidenced by an increase in the mRNA expression of MUC2,CDX2,and ***,ST-added FM resulted in decreased mRNA levels of TNF-α,IL-1β,and IL-6,and adhesion of *** to HT-29/MTX *** results suggest that consuming ST-added FM may help reinforce the intestinal mucus layer and prevent inflammations related to pathogenic bacteria.

关键词： Stevia Yogurt Fermented milk Lactic acid bacteria Mucin Bacterial adhesion

Effect of biochar on fate and transport of manure-borne estrogens in sandy soil

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Journal of Environmental Sciences 2018年第11期30卷 162-176页

作者： Sanaz Alizadeh Shiv O.Prasher Eman ElSayed Zhiming Qi Ramanbhai M.Patel department of bioresource Engineering McGill University department of Plant Protection Faculty of AgricultureZagazig University

The feasibility of using two types of biochars to reduce steroid hormone pollution from poultry and swine manure application on agricultural land was evaluated. The sorption affinity and desorption resistance of softwood and hardwood biochars were also determined for two estrogen hormones, 17β-estradiol （E2） and its primary metabolite estrone （E1）. The softwood and hardwood biochars demonstrated high retention capacity for both estrogens. The effective distribution coefficient （Kedff） of soil-softwood-derived biochar （SBS450） was significantly higher than soil-hardwood-derived biochar （SBH750）, indicating the stronger sorption affinity of SBS450 for estrogens. To validate the laboratory results, a field lysimeter experiment was conducted to study the fate and transport of E2 and E1 in soil and leachate in the presence of 1% softwood-biochar （BS450） in topsoil and to compare it with soil without any amendments. The spatio-temporal distribution of both estrogens was monitored at four depths over a 46-day period. The lysimeters, in which the surface layer of soil was amended with biochar, retained significantly higher concentrations of both estrogen hormones. Although they leached through the soil and were detected in leachates, collected at 1.0 m depth, the concentrations were significantly lower in the leachate collected from biochar-amended lysimeters. The result confirmed the efficacy of biochar amendment as a remediation technique to alleviate the manure-borne hormonal pollution of groundwater.

关键词： Estrogen Sorption Desorption Biodegradation Biochar Manure Transport

Three metabolites from an entomopathogenic bacterium, Xenorhabdus nematophila, inhibit larval development of Spodoptera exigua （Lepidoptera： Noctuidae） by inhibiting a digestive enzyme, phospholipase A2

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Insect Science 2011年第3期18卷 282-288页

作者： Jaehyun Kim Yonggyun Kim department of bioresource Sciences Andong National University Andong Korea

An entomopathogenic bacterium, Xenorhabdus nematophila, has been known to induce significant immunosuppression of target insects by inhibiting immune-associated phospholipase A2 （PLA2）, which subsequently shuts down biosynthesis of eicosanoids that are critical in immune mediation in insects. Some metabolites originated from the bacterial culture broth have been identified and include benzylideneacetone, proline-tyrosine and acetylated phenylalanine-glycine-valine, which are known to inhibit enzyme activity of PLA2 extracted from hemocyte and fat body. This study tested their effects on digestive PLA2 of the beet armyworm, Spodoptera exigua. Young larvae fed different concentrations of the three metabolites resulted in significant adverse effects on larval development even at doses below 100 #g/mL. In particular, they induced significant reduction in digestive efficiency of ingested food. All three metabolites significantly inhibited catalytic activity of digestive PLA2 extracted from midgut lumen of the fifth instar larvae at a low micromolar range. These results suggest that the inhibitory activities of the three bacterial metabolites on digestive PLA2 of S. exigua midgut may explain some of their oral toxic effects.

关键词： digestion midgut phospholipase A2 Spodoptera exigua Xenorhabdus nematophila

Accumulation of Pathogenesis-related Type-5 Like Proteins in Phytoplasma-infected Garland Chrysanthemum Chrysanthemum coronarium

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 773-779页

作者： Bo-Xiong ZHONG and Yan-Wei SHENdepartment of bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China department of bioresource Science College of Animal Science Zhejiang UniversityHangzhou 310029 China

Soluble proteins extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum plants infected by onion yellows phytoplasma were analyzed by two-dimensional gel electrophoresis. Computerized matching analysis revealed that at least six soluble proteins were accumulated specifically in phytoplasma-infected garland chrysanthemum. N-terminal amino acids sequences of these soluble

关键词： phytoplasma garland chrysanthemum Chrysanthemum coronarium pathogenesis-related proteins thaumatin osmotin

Immunosuppression induced by expression of a viral RNase enhances susceptibility of Plutella xylostella to microbial pesticides

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Insect Science 2012年第1期19卷 47-54页

作者： Bokri Park Yonggyun Kim department of bioresource Sciences Andong National University Andong South Korea

Polydnaviruses are a group of insect DNA viruses and are characterized in their segmented genome that is located in the chromosome（s） of host wasps. A polydnavirus, Cotesiaplutellae bracovirus （CpBV）, encodes a viral ribonuclease （RNase） T2 in a specific segment #3 （CpBV-S3）. This study tested its effect on gene expression associated with host immune responses in the diamondback moth, Plutella xylostella. Micro-inj ection of CpBV- $3 into nonparasitized larvae induced expression of its two encoded genes, CpBV-ORF301 （= CpBV-RNase T2） and CpBV-ORF302. In response to a bacterial challenge, four antimi- crobial peptide genes （hemolin, gloverin, cecropin and lysozyme） and six phenoloxidase （PO）-associated genes （proPO-activating proteinase, PO, serine proteinase homolog and serpins 1-3） were up-regulated in their expressions. However, the transient expression of CpBV-S3 suppressed the expressions of cecropin, PO and serpin 1. Double-stranded RNA specific to the viral RNase T2 could specifically knockdown the viral gene expression and restored the three gene expressions suppressed in the larvae injected with CpBV-S3. The inhibitory activity of the viral RNase T2 on the target genes was further proven by the suppression of PO activation in response to bacterial challenge in the larvae injected with CpBV-S3. This immunosuppression by the expression of the viral RNase T2 resulted in significant increase of pathogen susceptibility ofP. xylostella against Bacillus thuringiensis or baculovirus infection.

关键词： CpBV immune Plutella xylostella polydnavirus RNase T2 RNA interference transient expression

Role of phenolics as antioxidants,biomolecule protectors and as anti-diabetic factors-Evaluation on bark and empty pods of Acacia auriculiformis

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Asian Pacific Journal of Tropical Medicine 2012年第10期5卷 757-765页

作者： Arumugam Sathya Perumal Siddhuraju bioresource Technology Lab Department of Environmental SciencesBharathiar University

Objective:To search for an efficient and inexpensive source of phytoconstituents with antioxidant potential and health promoting traits from bark and empty pods of Acacia auriculiformis （***）.Methods:Samples of bark and empty pod extracts were analyzed for bioactives（phenolics,flavonoids and proanthocyanidins） and subjected to free radical scavenging activity on DPPH*,ABTS?,OH?,O2*,and NO along with the determination of reducing power, iron chelating activity and peroxidation *** action of extracts on biomolecules and cell membranes were evaluated by DNA nicking assay and haemolysis inhibition assay respectively,α-amylase andα-glucosidase inhibitory potentials were also determined. Results:All the bioactives analyzed were higher in bark（B） than empty pods（EP）[TPC:B （574.51±16.11）;EP（96.80±3.45） mg GAE/***:B（94.71±7.65）;EP（24（?）87±20.45） mg RE/g. Proanthocyanidins:B（2.81±0.31）;EP（1.25±0.01） mg LE/100 g DM]except *** the extracts showed higher quenching capacity on DPPH and ABTS（DPPH:B（0.21±0.01）;EP（1.51±0.17） g extract/g ***:B（111 519.14±79 340.91）;EP（80 232.55±32 894.12） mmol TE/g） with the FRAP of B（84 515.63±3 350.69） and EP（47 940.79±1 257.60） mmol Fe（（?））/*** chelation was not *** addition,they showed lower quenching activity on OH?（B（48.95±1.72）;EP（34.94±1.62）%） and equivalent quenching on O2?（B（53.47±3.92）;EP（24.41±2.61）%）,NO（B（49.04±5.04）; EP（51.00±5.13）%）,peroxidation inhibition（B（67.50±5.50）;EP（55.l±2.3）%） and antihaemolytic potential（B（87.60±6.84）%） towards authentic antioxidant ***,Empty pod extracts are devoid of antihaemolytic *** the extracts showed dose dependent DNA *** this,bark and empty pod extracts exhibited dual inhibiting potential againstα-amylase andα-glucosidase ***:On summarization,it insinuated that both bark and empty pods can be us

关键词： Phenolics Bark Empty pods Acacia auriculiformis Antioxidant activity TypeⅡdiabetes

Baculoviral p94 homologs encoded in Cotesia plutellae bracovirus suppress both immunity and development of the diamondback moth, Plutellae xylostella

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Insect Science 2016年第2期23卷 235-244页

作者： Yonggyun Kim Rahul Hepat department of bioresource Sciences Andong National University Andong 760-749 Korea

Polydnaviruses （PDVs） are a group of insect DNA viruses, which exhibit a mutual symbiotic relationship with their specific host wasps. Moreover, most encapsidated genes identified so far in PDVs share homologies with insect-originated genes, but not with virus-originated genes. In the meantime, PDVs associated with 2 wasp genera Cotesia and Glytapanteles encode some genes presumably originated from other viruses. Cotesia plutellae bracovirus （CpBV） encodes 4 genes homologous to baculoviral p94： CpBV- E94k1, CpB V-E94k2, CpB V-E94k3, and CpB V-E94k4. This study was conducted to predict the origin of CpB V-E94ks by comparing their sequences with those ofbaculoviral orthologs and to determine the physiological functions by their transient expressions in nonparasitized larvae and subsequent specific RNA interference. Our phylogenetic analysis indicated that CpBV-E94ks were clustered with other E94ks originated from different PDVs and shared high similarity with betabaculoviral p94s. These 4 CpBV genes were expressed during most developmental stages of the larvae of Plutella xylostella parasitized by C. plutellae. Expression of these 4 E94ks was mainly detected in hemocytes and fat body. Subsequent functional analysis by in vivo transient expression showed that all 4 viral genes significantly inhibited both host immune and developmental processes. These results suggest that CpB 11- E94ks share an origin with betabaculoviral p94s and play parasitic roles in suppressing host immune and developmental processes.

关键词： baculovirus Cotesia plutellae CpBV-E94k Plutella xylostella polyd-navirus

Insights into salvianolic acid B biosynthesis from chromosome-scale assembly of the Salvia bowleyana genome

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Journal of Integrative Plant Biology 2021年第7期63卷 1309-1323页

作者： Xuehai Zheng Duo Chen Binghua Chen Limin Liang Zhen Huang Wenfang Fan Jiannan Chen Wenjin He Huibin Chen Luqiang Huang Youqiang Chen Jinmao Zhu Ting Xue The Public Service Platform for industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration Fujian Key Laboratory of Special Marine Bioresource Sustainable UtilizationSouthern Institute of OceanographyCollege of Life SciencesFujian Normal UniversityFuzhou 350117China

Salvia bowleyanais a traditional Chinese medic-inal plant that is a source of nutritional supple-ments rich in salvianolic acid B and a potentialexperimental system for the exploration ofsalvianolic acid B biosynthesis in the ***,we report a high‐quality chromosome‐scalegenome assembly of *** covering 462.44 Mb,with a scaffold N50 value of 57.96 Mband 44,044 annotated protein‐coding *** analysis revealed an estimateddivergence time *** its close *** of~3.94 *** also observed evidence of a whole‐genome duplication in *** analysis showed that SbPAL1(PHENYLALANINE AMMONIA‐LYASE1)ishighlyexpressed in roots relative to stem and leaves,paralleling the location of salvianolic acid *** laccase gene family in *** anaoutnumbered their counterparts *** and Arabidopsis thaliana,suggesting that the gene family has undergoneexpansion *** laccasegenes were also highly expressed in roots,wheretheir encoded proteins may catalyze the oxida-tive reaction from rosmarinic acid to salvianolicacid *** provide an invaluable ge-nomic resource for understanding salvianolicacid B biosynthesis and its regulation,and will beuseful for exploring the evolution of the Labiatae.

关键词： chromosomal assembly gene family genomeevolution Salvia bowleyana salvianolic acid B whole‐genome

Rapid progress of DNA replication studies in Archaea,the third domain of life

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Science China(Life Sciences) 2012年第5期55卷 386-403页

作者： ISHINO Yoshizumi ISHINO Sonoko department of Bioscience and Biotechnology Graduate School of Bioresource and Bioenvironmental Sciences Kyushu University

Archaea,the third domain of life,are interesting organisms to study from the aspects of molecular and evolutionary *** cells have a unicellular ultrastructure without a nucleus,resembling bacterial cells,but the proteins involved in genetic information processing pathways,including DNA replication,transcription,and translation,share strong similarities with those of ***,archaea provide useful model systems to understand the more complex mechanisms of genetic information processing in eukaryotic ***,the hyperthermophilic archaea provide very stable proteins,which are especially useful for the isolation of replisomal multicomplexes,to analyze their structures and *** review focuses on the history,current status,and future directions of archaeal DNA replication studies.

关键词： Archaea DNA replication DNA polymerase replication fork replisome hyperthermophile

Cloning,expression in Escherichia coli,and enzymatic properties of laccase from Aeromonas hydrophila WL-11

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Journal of Environmental Sciences 2010年第4期22卷 635-640页

作者： Jing Wu Kyoung-Sook Kim Jai-Heon Lee Young-Choon Lee department of Biotechnology Dong-A University Busan 604-714 South Korea. Brain Korea 21 Center for Silver-Bio industrialization Dong-A University Busan 604-714 South Korea

A strain WL-11 with high laccase activity was isolated from activated sludge collected from the effluent treatment plant of a textile and dyeing industry. It was identified as Aeromonas hydrophila by physiological test and 16S rDNA sequence analysis. A gene encoding of laccase from a newly isolated Aeromonas hydrophila WL-11 was cloned and characterized. Nucleotide sequence analysis showed an open reading frame of 1605 bp encoding a polypeptide comprised of 534 amino acids. The primary structure of the enzyme predicted the structural features characteristic of other laccases, including the conserved regions of four histidine-rich copper-binding sites. The predicted amino acid sequence showed a high homology （more than 60%） with bacterial laccases in the genome and protein databases and the highest degree of similarity （61% identity） was observed with the multicopper oxidase of KlebsieUa sp. 601. When expressed in Escherichia coli, the recombinant enzyme was overproduced in the cytoplasm as soluble and active form. The purified enzyme had an optimum pH of 2.6 and 8.0 for ABTS （2,2＇-azino-bis（3-ethylbenzthiazolinesulfonic acid） and DMP （2,6-dimethoxyphenol）, respectively. The kinetic study on ABTS revealed a higher affinity of this enzyme to this substrate than DMP.

关键词： cloning expression enzymatic properties laccase Aeromonas hydrophila