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Constructing a biofunctionalized 3D-printed gelatin/sodium alginate/chitosan tri-polymer complex scaffold with improvised biological andmechanical properties for bone-tissue engineering

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Bio-Design and Manufacturing 2024年第1期7卷 57-73页

作者： Amit Kumar Singh Krishna Pramanik Amit Biswas Centre of Excellence in Tissue engineering Department of Biotechnology and Medical EngineeringNational Institute of TechnologyRourkela 769008India

Sodium alginate(SA)/chitosan(CH)polyelectrolyte scaffold is a suitable substrate for tissue-engineering application.The present study deals with further improvement in the tensile strength and biological properties of this type of scaffold to make it a potential template for bone-tissue regeneration.We experimented with adding 0%–15%(volume fraction)gelatin(GE),a protein-based biopolymer known to promote cell adhesion,proliferation,and differentiation.The resulting tri-polymer complex was used as bioink to fabricate SA/CH/GEmatrices by three-dimensional(3D)printing.Morphological studies using scanning electron microscopy revealed the microfibrous porous architecture of all the structures,which had a pore size range of 383–419μm.X-ray diffraction and Fourier-transform infrared spectroscopy analyses revealed the amorphous nature of the scaffold and the strong electrostatic interactions among the functional groups of the polymers,thereby forming polyelectrolyte complexes which were found to improve mechanical properties and structural stability.The scaffolds exhibited a desirable degradation rate,controlled swelling,and hydrophilic characteristics which are favorable for bone-tissue engineering.The tensile strength improved from(386±15)to(693±15)kPa due to the increased stiffness of SA/CH scaffolds upon addition of gelatin.The enhanced protein adsorption and in vitro bioactivity(forming an apatite layer)confirmed the ability of the SA/CH/GE scaffold to offer higher cellular adhesion and a bone-like environment to cells during the process of tissue regeneration.In vitro biological evaluation including the MTT assay,confocal microscopy analysis,and alizarin red S assay showed a significant increase in cell attachment,cell viability,and cell proliferation,which further improved biomineralization over the scaffold surface.In addition,SA/CH containing 15%gelatin designated as SA/CH/GE15 showed superior performance to the other fabricated 3D structures,demonstrating its potential for use in bone-tissue engineering.

关键词： Scaffold Biomaterial Sodium alginate Chitosan Gelatin 3D printing Tissue engineering

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Design of a Novel Electrochemical Aptasensor Based on Molybdenum Disulfide Nanosheets for Lysozyme Detection

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Journal of Analysis and Testing 2024年第1期8卷 16-27页

作者： Tavoos Beiki Ghasem Najafpour-Darzi Maedeh Mohammadi Mohsen Shakeri biotechnology Research Laboratory Department of Chemical EngineeringBabol Noshirvani University of TechnologyBabol 47148-71167Iran department of Mechanical engineering Babol Noshirvani University of TechnologyBabol 47148-71167Iran

The precise detection of lysozyme plays a crucial role in early diagnosis of kidney failure in leukemia patients.Herein,we designed an electrochemical aptasensor to detect lysozyme using a glassy carbon electrode(GCE)modified with molybdenum disulfide(MoS_(2))nanosheets decorated with chitosan(Ch)and gold nanoparticles(Au).In the next steps of aptasensor preparation,aptamer(Apt)strands were immobilized on MoS_(2)-Au-Ch/GCE surface via electrostatic interactions,and bovine serum albumin(BSA)solution was applied to cover unbonded areas on Apt/MoS_(2)-Au-Ch/GCE.The MoS_(2)-Au-Ch nanocomposite can warrant enhanced immobilization of aptamers and strong signals because of wrinkle-like structure and base planes in MoS nanosheets,presence of abundant amino and carboxyl functional groups in chitosan and Au nanoparticles.Differential pulse voltammetry(DPV)was used to evaluate the electrochemical behavior of the aptasensor at each preparation step and its detection performance.The developed electrochemical aptasensor based on MoS nanosheets showed remarkable performance for lysozyme detection in the range from 1 pmol/L to 10 nmol/L with a limit of detection of 2.2 fmol/L.The proposed aptasensor provided excellent selectivity against BSA,uric acid,cytochrome C,lysozyme and their mixture.In addition,it displayed great stability of about 99.58%of the initial signal upon 21-day storage.Tests on human serum and urine samples yielded promising recoveries ranging from 98 to 105%.Hence,MoS_(2)-Au-Ch nanocomposite-based aptasensor could be an outstanding candidate for medical device applications and protein quantification.

关键词： Electrochemical aptasensor Lysozyme MoS_(2)nanosheets Apt/MoS_(2)-Au-Ch/GCE

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Metal-organic framework hybrid materials of ZIF-8/RGO for immobilization of D-amino acid dehydrogenase

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Nano Research 2024年第1期17卷 290-296页

作者： Hangbin Lei Qian Zhang Xiaoyan Xiang Liang Jiang Shiyan Wang Lingxuan Duan Shizhen Wang department of Chemical and Biochemical engineering College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China Xiamen Key Laboratory of Synthetic biotechnology Xiamen UniversityXiamen 361005China

Immobilization of D-amino acid dehydrogenase(DAADH)by the assembly of peptide linker was studied for the biosynthesis of Dphenylalanine.Hybrid material of zeolitic imidazolate framework-8(ZIF-8)combined with reduced graphene oxide(RGO)was applied for the immobilization of DAADH from Ureibacillus thermosphaericus.The recovery rate of DAADH/ZIF-8/RGO was 165.6%.DAADH/ZIF-8/RGO remained 53.4%of its initial activity at 50°C for 10 h while the free enzyme was inactivated.DAADH/ZIF-8/RGO maintained 70.5%activity in hyperalkaline solution with pH 12.Kinetic parameters indicated that DAADH/ZIF-8/RGO had greater affinity of phenylpyruvate as V_(max)/K_(m)of DAADH/ZIF-8/RGO was 1.27-fold than free enzyme.After seven recycles,the activity of DAADH/ZIF-8/RGO remained 64.3%.Furthermore,one-step separation and in situ immobilization of DAADH by ZIF-8/RGO/Ni was carried out with 1.5-fold activity enhancement.Combining peptide linker and metal-organic framework(MOF)immobilization,thermostability and activity of the immobilized DAADH were significantly improved.

关键词： D-Amino acid dehydrogenase immobilization metal-organic frameworks zeolitic imidazolate framework-8(ZIF-8) reduced graphene oxide

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Managing the surge:A comprehensive review of the entire disposal framework for retired lithium-ion batteries from electric vehicles

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Journal of Energy Chemistry 2024年第5期92卷 648-680页

作者： Ruohan Guo Feng Wang M.Akbar Rhamdhani Yiming Xu Weixiang Shen department of engineering Technologies School of ScienceComputing and Engineering TechnologiesSwinburne University of TechnologyMelbourne VIC 3122Australia department of Chemistry and biotechnology School of ScienceComputing and Engineering TechnologiesSwinburne University of TechnologyMelbourne VIC 3122Australia department Mechanical and Product Design engineering School of EngineeringSwinburne University of TechnologyMelbourne VIC 3122Australia

Anticipating the imminent surge of retired lithium-ion batteries(R-LIBs)from electric vehicles,the need for safe,cost-effective and environmentally friendly disposal technologies has escalated.This paper seeks to offer a comprehensive overview of the entire disposal framework for R-LIBs,encompassing a broad spectrum of activities,including screening,repurposing and recycling.Firstly,we delve deeply into a thorough examination of current screening technologies,shifting the focus from a mere enumeration of screening methods to the exploration of the strategies for enhancing screening efficiency.Secondly,we outline battery repurposing with associated key factors,summarizing stationary applications and sizing methods for R-LIBs in their second life.A particular light is shed on available reconditioning solutions,demonstrating their great potential in facilitating battery safety and lifetime in repurposing scenarios and identifying their techno-economic issues.In the realm of battery recycling,we present an extensive survey of pre-treatment options and subsequent material recovery technologies.Particularly,we introduce several global leading recyclers to illustrate their industrial processes and technical intricacies.Furthermore,relevant challenges and evolving trends are investigated in pursuit of a sustainable end-of-life management and disposal framework.We hope that this study can serve as a valuable resource for researchers,industry professionals and policymakers in this field,ultimately facilitating the adoption of proper disposal practices.

关键词： Lithium-ion battery Battery reproposing and recycling Miaieiials recovery technologies Techno-economic issues End-of-life management Disposal framework

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Investigating the impact of dynamic structural changes of Au/rutile catalysts on the catalytic activity of CO oxidation

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Carbon Energy 2024年第4期6卷 222-232页

作者： Xiaochun Hu Qianwenhao Fan Mingwu Tan Yuqing Luo Xianyue Wu Manoel Y.Manuputty Jie Ding Tej SChoksi Markus Kraft Rong Xu Zhiqiang Sun Wen Liu School of Energy Science and engineering Central South UniversityChangshaChina School of Chemistry Chemical Engineering and BiotechnologyNanyang Technological UniversitySingaporeSingapore Cambridge Centre for Advanced Research and Education SingaporeSingapore Institute of Sustainability for Chemicals Energy and EnvironmentJurong IslandSingapore College of Chemistry and Chemical engineering Central South UniversityChangshaChina department of Chemical engineering and biotechnology University of CambridgeCambridgeUK

The surface properties of oxidic supports and their interaction with the supported metals play critical roles in governing the catalytic activities of oxide‐supported metal catalysts.When metals are supported on reducible oxides,dynamic surface reconstruction phenomena,including strong metal–support interaction(SMSI)and oxygen vacancy formation,complicate the determination of the structural–functional relationship at the active sites.Here,we performed a systematic investigation of the dynamic behavior of Au nanocatalysts supported on flame‐synthesized TiO_(2),which takes predominantly a rutile phase,using CO oxidation above room temperature as a probe reaction.Our analysis conclusively elucidated a negative correlation between the catalytic activity of Au/TiO_(2) and the oxygen vacancy at the Au/TiO_(2) interface.Although the reversible formation and retracting of SMSI overlayers have been ubiquitously observed on Au/TiO_(2) samples,the catalytic consequence of SMSI remains inconclusive.Density functional theory suggests that the electron transfer from TiO_(2) to Au is correlated to the presence of the interfacial oxygen vacancies,retarding the catalytic activation of CO oxidation.

关键词： CO oxidation electronic metal-support interactions flame‐synthesis metal-support interactions oxygen vacancy strong metal-support interaction

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Experimental measurement and numerical modeling of deformation behavior of breast cancer cells passing through constricted microfluidic channels

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Microsystems & Nanoengineering 2024年第1期10卷 129-147页

作者： Pouyan Keshavarz Motamed Hesam Abouali Mahla Poudineh Nima Maftoon department of Systems Design engineering University of WaterlooWaterlooON N2L 3G1Canada Center for Bioengineering and biotechnology University of WaterlooWaterlooON N2L 3G1Canada department of Electrical and Computer engineering University of WaterlooWaterlooON N2L 3G1Canada

During the multistep process of metastasis,cancer cells encounter various mechanical forces which make them deform drastically.Developing accurate in-silico models,capable of simulating the interactions between the mechanical forces and highly deformable cancer cells,can pave the way for the development of novel diagnostic and predictive methods for metastatic progression.Spring-network models of cancer cell,empowered by our recently proposed identification approach,promises a versatile numerical tool for developing experimentally validated models that can simulate complex interactions at cellular scale.Using this numerical tool,we presented spring-network models of breast cancer cells that can accurately replicate the experimental data of deformation behavior of the cells flowing in a fluidic domain and passing narrow constrictions comparable to microcapillary.First,using high-speed imaging,we experimentally studied the deformability of breast cancer cell lines with varying metastatic potential(MCF-7(less invasive),SKBR-3(medium-high invasive),and MDA-MB-231(highly invasive)in terms of their entry time to a constricted microfluidic channel.We observed that MDA-MB-231,that has the highest metastatic potential,is the most deformable cell among the three.Then,by focusing on this cell line,experimental measurements were expanded to two more constricted microchannel dimensions.The experimental deformability data in three constricted microchannel sizes for various cell sizes,enabled accurate identification of the unknown parameters of the spring-network model of the breast cancer cell line(MDA-MB-231).Our results show that the identifed parameters depend on the cell size,suggesting the need for a systematic procedure for identifying the size-dependent parameters of spring-network models of cells.As the numerical results show,the presented cell models can simulate the entry process of the cell into constricted channels with very good agreements with the measured experimental data.

关键词： strict passing numerical

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Recent Advances in Catalytic Carbonylation Reactions in Alternative Reaction Media

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Chinese Journal of Chemistry 2024年第4期42卷 406-429页

作者： Csaba Árvai László T.Mika department of Chemical and Environmental Process engineering Faculty of Chemical Technology and BiotechnologyBudapest University of Technology and EconomicsMűegyetem rkp.3H-1111 BudapestHungary

Since the discovery of the hydroformylation(oxo-synthesis or Roelen reaction)and the Reppe-reaction,the transition metal-catalyzed carbonylation reactions,providing versatile,facile,and even atom-economic methods for the selective incorporation of C=O functionality to various skeletons,have gained tremendous importance in synthetic organic chemistry from laboratories to industrial applications.The carbonylation of carbon-carbon multiple bonds,aromatic halides,triflates,etc.,in the presence of various nucleophiles has led the way to produce aldehydes,carboxylic acids,esters,amides,etc.,in the fine chemical industry.However,these protocols usually proceed in conventional,fossil-based,and usually toxic reaction environments.Thus,several attempts have been directed to developing efficient carbonylation methods in alternative,less harmful,non-fossil-based and even in renewable reaction media.In this review,we overview the recent applications of alternative solvents such as water,biomass-based alcohols,γ-valerolactone(GVL),2-methyltetrahydrofuran(2-MeTHF),ionic liquids(ILs),deep eutectic solvents(DES),alkyl levulinates,limonene,α-pinene,and dimethyl carbonate as well as fluorous media to improve efficiency,safety and environmentally benign nature of carbonylation protocols.

关键词： Carbonylation Catalysis Solvents Green chemistry Carbon monoxide Transition metals Homogeneous catalysis Palladium

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Efficient ammonia production over e_(g)-occupancy-optimized perovskite electrocatalysts

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Nano Research 2024年第6期17卷 4682-4686页

作者： Mingfa Chen Yu Zhang Fulong Liu Zhenbao Zhang Yuming Dong Yongfa Zhu Heqing Jiang Jiawei Zhu Key Laboratory of Synthetic and Biological Colloids Ministry of EducationSchool of Chemical and Material EngineeringJiangnan UniversityWuxi 214122China Qingdao Institute of Bioenergy and bioprocess Technology Chinese Academy of SciencesQingdao 266101China School of Chemistry and Chemical engineering Linyi UniversityLinyi 276005China department of Chemistry Tsinghua UniversityBeijing 100084China

Renewable-energy-driven nitrate(NO_(3)^(−))electroreduction to ammonia(NH_(3))(NERA)has been an attractive technology for decarbonizing NH_(3)production and wastewater treatment.Improving NERA efficiency requires electrocatalysts that are earth-abundant and show fantastic performance.Here we report a semiempirical activity descriptor of eg occupancy(of surface B-site cations)for identifying inexpensive perovskite oxides with extremely high efficacy toward NERA.We establish the descriptor by systematic investigations of more than 10 perovskite oxides.These investigations demonstrate that their intrinsic NERA activities display a volcano-shaped dependence on eg occupancy and the optimized intrinsic activities are accessible at near-1 eg occupancies.This could plausibly be attributed to the favorable overlaps between surface adsorbates and vertically-oriented eg orbitals.More importantly,utilizing this descriptor,we predict a highly active,selective,and durable NERA electrocatalyst with a composition of Ba_(0.5)Sr_(0.5)Co_(0.8)Fe_(0.2)O_(3−δ)(BSCF).Because of its close-to-1 e_(g)occupancy(i.e.~1.2),the BSCF features a superior NH_(3)production rate of 0.12 g·h^(−1)·mg_(cat.)^(−1)(Faradaic efficiency of 97.8%)that is at top of the volcano plot,and substantially outperforms most NERA electrocatalysts reported in literature.

关键词： perovskite oxide nitrate electroreduction ammonia electrosynthesis eg occupancy

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Bioactive compounds in Hericium erinaceus and their biological properties:a review

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Food Science and Human Wellness 2024年第4期13卷 1825-1844页

作者： Yue Qiu Genglan Lin Weiming Liu Fuming Zhang Robert J.Linhardt Xingli Wang Anqiang Zhang College of Food Science and engineering Zhejiang University of TechnologyHangzhou 310014China Zhejiang Biosan Biotech Co. Ltd.Hangzhou 310052China department of Chemical and Biological engineering Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyNY 12180USA departments of Chemistry and Chemical Biology and Biomedical engineering Biological ScienceCenter for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyNY 12180USA

Hericium erinaceus is a nutritious edible and medicinal fungi,rich in a variety of functional active ingredients,with various physiological functions such as antioxidation,anticancer,and enhancing immunity.It is also effective in protecting the digestive system and preventing neurodegenerative diseases.In this review paper,we summarize the sources,structures and efficacies of the main active components in H.erinaceus fruiting body,mycelium,and culture media,and update the latest research progress on their biological activities and the related molecular mechanisms.Based on this information,we provide detailed challenges in current research,industrialization and information on the active ingredients of H.erinaceus.Perspectives for future studies and new applications of H.erinaceus are proposed.

关键词： Hericium erinaceus Bioactive compounds Biological activities Antioxidation Neuroprotection Immunoregulation

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Harnessing endothelial cells and vascularization strategies for nerve regeneration

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Neural Regeneration Research 2024年第11期19卷 2337-2338页

作者： Papon Muangsanit Poppy Smith Virology and Cell Technology Research Team National Center for Genetic Engineering and Biotechnology(BIOTEC)National Science and Technology Development Agency(NSTDA)PathumthaniThailand UCL Centre for Nerve engineering UCLLondonUK department of Pharmacology UCL School of PharmacyUCLLondonUK

Peripheral nerves are essential components of the human body’s communication system,transmitting signals between the central nervous system and various body parts.Damage resulting from trauma or disease can result in debilitating sensory and motor deficits.Nerve injuries,particularly those resulting in significant gaps in the nerve tissue,pose a formidable challenge for clinicians and researchers.Despite their limitations,including limited availability and donor site morbidity,nerve autografts remain the clinical gold standard for treating nerve injuries.

关键词： injuries nerves donor

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