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science China Life sciences 2024年第9期67卷 1970-1988页

作者： Jiayi Yu Changbing Zheng Qiuping Guo Yulong Yin Yehui Duan Fengna Li CAS Key Laboratory of Agro-ecological Processes in Subtropical Region Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Institute of Subtropical Agriculture Chinese Academy of Sciences University of Chinese Academy of sciences College of animal science and Technology Hunan Agricultural University

We previously demonstrated that lipopolysaccharide(LPS) injection-induced immune stress could impair muscle growth in weaned piglets, but the precise mechanisms behind this remain elusive. Here, we found that chronic immune stress induced by LPS resulted in a significant reduction of 36.86% in the total muscle mass of piglets at 5 d post-treatment compared with the control group. At 1 d,prior to muscle mass loss, multiple alterations were noted in response to LPS treatment. These included a reduction in the abundance of Bacteroidetes, an increase in serum concentrations of pro-inflammatory cytokines, compromised mitochondrial morphology, and an upregulation in the expression of dynamin-related protein 1(Drp1), a critical protein involved in mitochondrial fission. We highlight a strong negative correlation between Bacteroidetes abundance and the levels of serum pro-inflammatory cytokines, corroborated by in vivo intervention strategies in the musculature of both pig and mouse models. Mechanistically, the effects of Bacteroidetes on inflammation and muscle mass loss may involve the signaling pathway of the tauro-β-muricholic acid-fibroblast growth factor 15. Furthermore, the induction of overexpression of inflammatory cytokines, achieved without LPS treatment through oral administration of recombinant human IL-6(rh IL-6), led to increased levels of circulating cytokines, subsequently causing a decrease in muscle mass. Notably, pre-treatment with Mdivi-1, an inhibitor of Drp-1, markedly attenuated the LPS-induced elevation in reactive oxygen species levels and rescued the associated decline in muscle mass. Collectively, these data indicate that LPS-induced muscle mass loss was linked to the reduction of Bacteroidetes abundance, increased inflammation, and the disruption of mitochondrial morphology. These insights offer promising avenues for the identification of potential therapeutic targets aimed at mitigating muscle mass loss.

关键词： Bacteroidetes inflammation mitochondrial morphology muscle mass loss piglets

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Dietary supplementation of laminarin improves the reproductive performance of sows and the growth of suckling piglets

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Journal of animal science and Biotechnology 2024年第1期15卷 329-343页

作者： Pengguang Zhang Guoyuan Jiang Chenghong Ma Yubo Wang Enfa Yan Linjuan He Jianxin Guo Xin Zhang Jingdong Yin State Key Laboratory of animal Nutrition and Feeding College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background Maternal nutrition is essential in keeping a highly efficient production system in the pig *** has been shown to improve antioxidant capacity,reduce the inflammatory response,and favor the homeostasis of intestinal ***,the effect of dietary supplementation of laminarin on the reproductive performance of sows and the growth of suckling offspring remains *** A total of 40 Landrace×Yorkshire multiparous sows on d 85 of gestation,similar in age,body weight(BW),parity and reproductive performance,were randomly divided into four dietary treatments with 10 sows per treatment,receiving a control diet(basal pregnancy or lactating diets)and a basal diet supplemented with 0.025%,0.05%and 0.10%laminarin,*** experiment lasted from d 85 of gestation to d 21 of *** Laminarin supplementation linearly increased number born alive per litter(P=0.03),average daily feed intake(ADFI,P<0.01),and total milk yield of sows during the lactation of 1–21 d(P=0.02).Furthermore,maternal laminarin supplementation increased the average daily gain(ADG)of piglets while tending to reduce the culling and death rate before *** addition,alterations to the composition of colostrum and milk,as well as to serum inflammatory cytokines and immunoglobulins of sows were *** fecal microbiota profile of sows supported the improvement of reproductive performance in sows and the growth performance in suckling *** Dietary supplementation of laminarin during late pregnancy and lactation could significantly improve reproductive performance of sows and growth performance of piglets.

关键词： Fecal microbiota Laminarin Milk Piglets Reproductive performance Sows

Proteo-transcriptomic profiles reveal key regulatory pathways and functions of LDHA in the ovulation of domestic chickens(Gallus gallus)

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Journal of animal science and Biotechnology 2024年第4期15卷 1449-1465页

作者： Ruixue Nie Wenhui Zhang Haoyu Tian Junying Li Yao Ling Bo Zhang Hao Zhang Changxin Wu State Key Laboratory of animal Biotech Breeding Beijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background In poultry, the smooth transition of follicles from the preovulatory-to-postovulatory phase impacts egg production in hens and can benefit the poultry industry. However, the regulatory mechanism underlying follicular ovulation in avians is a complex biological process that remains *** Critical biochemical events involved in ovulation in domestic chickens(Gallus gallus) were evaluated by transcriptomics, proteomics, and in vitro assays. Comparative transcriptome analyses of the largest preovulatory follicle(F1) and postovulatory follicle(POF1) in continuous laying(CL) and intermittent laying(IL) chickens indicated the greatest difference between CL_F1 and IL_F1, with 950 differentially expressed genes(DEGs), and the smallest difference between CL_POF1 and IL_POF1, with 14 DEGs. Additionally, data-independent acquisition proteomics revealed 252 differentially abundant proteins between CL_F1 and IL_F1. Perivitelline membrane synthesis, steroid biosynthesis, lysosomes, and oxidative phosphorylation were identified as pivotal pathways contributing to ovulation regulation. In particular, the regulation of zona pellucida sperm-binding protein 3, plasminogen activator, cathepsin A, and lactate dehydrogenase A(LDHA) was shown to be essential for ovulation. Furthermore, the inhibition of LDHA decreased cell viability and promoted apoptosis of ovarian follicles in *** This study reveals several important biochemical events involved in the process of ovulation, as well as crucial role of LDHA. These findings improve our understanding of ovulation and its regulatory mechanisms in avian species.

关键词： Chicken Data-independent acquisition proteomics LDHA Ovulation Regulatory mechanism Transcriptome

Fairy shrimp Branchinella kugenumaensis displays sensitivity to microplastic exposure

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Journal of Oceanology and Limnology 2024年第4期42卷 1186-1199页

作者： Yingying ZHANG Xinrui XU Wenbo SUN Yuting WANG Hui YANG College of animal science and Technology Yangzhou UniversityYangzhou 225009China

The increasing global concern regarding plastic pollution has prompted the research into the consequences of microplastics(MPs)on aquatic *** shrimp Branchinella kugenumaensis are freshwater planktonic organisms that have existed for 250 million *** study aimed to uncover the harmful effects of MPs,with a particular focus on their size variations(0.1,1,and 5μm),on the fairy *** focused on how MPs could significantly affect the survival and growth of fairy ***,larger MPs,especially those measuring 5μm,caused higher mortality rates and hindered the growth compared to smaller *** impact of MPs continued even subsequent to depuration in clean *** accumulation of MPs within the intestines of fairy shrimp resulted in intestinal blockages,disrupted excretory functions,and harmed intestinal epithelial *** at the histological,cellular,and molecular levels showed that exposure to MPs triggered necroptosis in intestinal cells,accompanied by alterations in pathways related to transcription,translation,digestion,energy metabolism,and neurological ***,the effects of MPs on gene expression and pathways varied based on particle size,with larger MPs having a more significant effect and causing a strong response in xenobiotic biodegradation and metabolism *** suggest that the increasing severity of MPs pollution could pose a significant threat to the survival of fairy *** study provided vital insights into the complex relationship between microplastics and aquatic organisms,and highlighted the urgent need to address the potential devastating impact of plastic pollution on freshwater ***,due to their rapid growth,strong reproductive capacity,sensitivity,and ease of cultivation,fairy shrimp hold the potential candidate to serve as a model organism for studying the effects of MPs and other pollutants on freshwater ecosystems.

关键词： microplastic freshwater ecosystem toxic effects fairy shrimp model organism

The chemical characteristics of different sodium iron ethylenediaminetetraacetate sources and their relative bioavailabilities for broilers fed with a conventional corn‑soybean meal diet

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Journal of animal science and Biotechnology 2024年第2期15卷 826-843页

作者： Shengchen Wang Bingxin Wu Ling Zhu Weiyun Zhang Liyang Zhang We Wu Jiaqi Wu Yun Hu Tingting Li Xiaoyan Cui Xugang Luo College of animal science and Technology Yangzhou UniversityYangzhou 225000People’s Republic of China Mineral Nutrition Research Division State Key Laboratory of Animal NutritionInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China

Background Our previous studies demonstrated that divalent organic iron(Fe)proteinate sources with higher complexation or chelation strengths as expressed by the greater quotient of formation(Qf)values displayed higher Fe bioavailabilities for *** iron ethylenediaminetetraacetate(NaFeEDTA)is a trivalent organic Fe source with the strongest chelating ligand ***,the bioavailability of Fe when administered as NaFeEDTA in broilers and other agricultural animals remains ***,the chemical characteristics of 12 NaFeEDTA products were *** these,one feed grade NaFeEDTA(Qf=2.07×10^(8)),one food grade NaFeEDTA(Qf=3.31×10^(8)),and one Fe proteinate with an extremely strong chelation strength(Fe-Prot ES,Qf value=8,590)were *** bioavailabilities relative to Fe sulfate(FeSO_(4)·7H_(2)O)for broilers fed with a conventional corn-soybean meal diet were evaluated during d 1 to 21 by investigating the effects of the above Fe sources and added Fe levels on the growth performance,hematological indices,Fe contents,activities and gene expressions of Fe-containing enzymes in various tissues of *** NaFeEDTA sources varied greatly in their chemical *** Fe concentration(PI),transferrin saturation(TS),liver Fe content,succinate dehydrogenase(SDH)activities in liver,heart,and kidney,catalase(CAT)activity in liver,and SDH mRNA expressions in liver and kidney increased linearly(P<0.05)with increasing levels of Fe ***,differences among Fe sources were detected(P<0.05)only for PI,liver Fe content,CAT activity in liver,SDH activities in heart and kidney,and SDH mRNA expressions in liver and *** on slope ratios from multiple linear regressions of the above indices on daily dietary analyzed Fe intake,the average bioavailabilities of Fe-Prot ES,feed grade NaFeEDTA,and food grade NaFeEDTA relative to the inorganic FeSO_(4)·7H_(2)O(100%)for broilers were 139%,155%,and 166%,***

关键词： Broilers Chelation strengths Fe-containing enzymes NaFeEDTA Relative bioavailabilities

Imprinting at the KBTBD6 locus involves species-specific m ternal methylation and monoallelic expression in livestock animals

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Journal of animal science and Biotechnology 2024年第1期15卷 127-145页

作者： Jinsoo Ahn In-Sul Hwang Mi-Ryung Park Seongsoo Hwang Kichoon Lee department of animal sciences The Ohio State UniversityColumbusOH 43210USA animal Biotechnology Division Rural Development AdministrationNational Institute of Animal ScienceJeonbuk 55365Republic of Korea Columbia Center for Translational Immunology Columbia University Irving Medical CenterColumbia UniversityNew YorkNY 10032USA animal Welfare Research Team National Institute of Animal ScienceRDA1500Kongjwipatjwi‑roJeollabuk‑do 55365Republic of Korea

Background The primary differentially methylated regions(DMRs) which are maternally hypermethylated serve as imprinting control regions(ICRs) that drive monoallelic gene expression, and these ICRs have been investigated due to their implications in mammalian development. Although a subset of genes has been identified as imprinted, in-depth comparative approach needs to be developed for identification of species-specific imprinted genes. Here, we examined DNA methylation status and allelic expression at the KBTBD6 locus across species and tissues and explored potential mechanisms of *** Using whole-genome bisulfite sequencing and RNA-sequencing on parthenogenetic and normal porcine embryos, we identified a maternally hypermethylated DMR between the embryos at the KBTBD6 promoter Cp G island and paternal monoallelic expression of KBTBD6. Also, in analyzed domesticated mammals but not in humans, non-human primates and mice, the KBTBD6 promoter Cp G islands were methylated in oocytes and/or allelically methyl-ated in tissues, and monoallelic KBTBD6 expression was observed, indicating livestock-specific imprinting. Further analysis revealed that these Cp G islands were embedded within transcripts in porcine and bovine oocytes which coexisted with an active transcription mark and DNA methylation, implying the presence of transcription-dependent *** In this study, our comparative approach revealed an imprinted expression of the KBTBD6 gene in domesticated mammals, but not in humans, non-human primates, and mice which implicates species-specific evolution of genomic imprinting.

关键词： Differentially methylated region Domesticated mammal Imprinting KBTBD6 Parthenogenetic

Responses of growth performance,antioxidant function,small intestinal morphology and mRNA expression of jejunal tight junction protein to dietary iron in yellow-feathered broilers

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Journal of Integrative Agriculture 2024年第4期23卷 1329-1337页

作者： Kaiwen Lei Hao Wu Jerry W Spears Xi Lin Xi Wang Xue Bai Yanling Huang Key Laboratory of animal science of State Ethnic Affairs Commission Southwest Minzu UniversityChengdu 610041China Key Laboratory of Qinghai-Tibetan Plateau animal Genetic Resource Reservation and Utilization Southwest Minzu UniversityChengdu 610041China department of animal science North Carolina State UniversityRaleighNC 27695-7621USA

This study aimed to investigate the dose-effect of iron on growth performance,antioxidant *** morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers.A total of 7201-d-old yellow-feathered maleb roilers were allocated to 9 treatments with 8 replicate cages of 10 birds per *** dietary treatments were consisted of a basal diet(contained 79.6 mg Fe kg^(-1))supplemented with 0,20,40,60,80,160,320,640,and 1,280 mg Fe kg^(-1)in the form of FeSO_(4)·7H_(2)*** with the birds in the control group,birds supplemented with 20mg Fe kg^(-1)had higher average daily gain(ADG)(P<0.0001).Adding 640 and 1,280 mg Fe kg^(-1)significantly decreased ADG(P<0.0001)and average daily feed intake(ADFI)(P<0.0001)compared with supplementation of 20mg Fe kg^(-1).Malondialdehyde(MDA)concentration in plasma and duodenum increased linearly(P<0.0001),but MDA concentration in liver and jejunum increased linearly(P<0.05)or quadratically(P<0.05)with increased dietary Fe *** villus height(VH)in duodenum and jejunum,and the ratio of villus height to crypt depth(V/C)in duodenum decreased linearly(P?0.05)as dietary *** dietary Fe increased,the jejunal relative mRNA abundance of claudin-1 decreased linearly(P=0.001),but the jejunal relative mRNA abundance of zona occludens-1(ZO-1)and occludin decreased linearly(P?0.05)or quadratically(P?0.05).Compared with the supplementation of 20 mg Fe kg^(-1),the supplementation of640 mg Fe kg^(-1)or higher increased(P?0.05)MDA concentrations in plasma,duodenum,and jejunum,decreased VH in the duodenum and jejunum,and the addition of 1,280 mg Fe kg^(-1)reduced(P?0.05)the jejunal tight junction protein(claudin-1,ZO-1,occludin)mRNA *** summary,640 mg of supplemental Fe kg^(-1)or greater was associated with decreased growth performance,increased oxidative stress,disrupted intestinal morphology,and reduced mRNA expression of jejunal tight junction protein.

关键词： iron yellow-feathered broiler antioxidant function intestinal morphology tight junction protein

Diarrhea induced by insufficient fat absorption in weaned piglets:Causes and nutrition regulation

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animal Nutrition 2024年第1期16卷 299-305页

作者： Yuying Li Pengjun Shi Kang Yao Qian Lin Mansheng Wang Zhenping Hou Wenjie Tang Hui Diao Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410205China Institute of Subtropical Agriculture Chinese Academy of SciencesHunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic ProcessChangsha 410125China animal Breeding and Genetics Key Laboratory of Sichuan Province Livestock and Poultry Biological Products Key Laboratory of Sichuan ProvinceSichuan Animal Science AcademySichuan Animtech Feed Co.LtdChengdu 610066China

Fat is one of the three macronutrients and a significant energy source for *** plays a positive role in maintaining intestinal health and improving production *** the weaning period,physiological,stress and diet-related factors influence the absorption of fat in piglets,leading to damage to the intestinal barrier,diarrhea and even *** pathways,such as fatty acid translocase(CD36),pregnane X receptor(PXR),and AMP-dependent protein kinase(AMPK),are responsible for regulating intestinal fat uptake and maintaining intestinal barrier ***,this review mainly elaborates on the reasons for diarrhea induced by insufficient fat absorption and related signaling pathways in weaned-piglets,with an emphasis on the intestinal fat absorption ***,we focus on introducing nutritional strategies that can promote intestinal fat absorption in piglets with insufficient fat absorption-related diarrhea,such as lipase,amino acids,and probiotics.

关键词： Weaned piglet Diarrhea Fat absorption Nutrient regulation

Repeated inoculation with rumen fluid accelerates the rumen bacterial transition with no benefit on production performance in postpartum Holstein dairy cows

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Journal of animal science and Biotechnology 2024年第2期15卷 795-811页

作者： Fanlin Kong Feiran Wang Yijia Zhang Shuo Wang Wei Wang Shengli Li Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control State Key Laboratory of Animal NutritionDepartment of Animal Nutrition and Feed ScienceCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193PR China College of Veterinary Medicine Nanjing Agricultural UniversityNanjingJiangsu 210095PR China

Background The dairy cow’s postpartum period is characterized by dramatic physiological changes,therefore imposing severe challenges on the animal for maintaining health and milk *** dynamics of the ruminal microbiota are also tremendous and may play a crucial role in lactation *** aim to investigate the potential benefits of early microbial intervention by fresh rumen microbiota transplantation(RMT)and sterile RMT in postpartum dairy *** fistulated peak-lactation dairy cows were selected to be the donors for rumen fluid *** postpartum cows were divided into 3 groups as the transplantation receptors respectively receiving 10 L fresh rumen fluid(FR),10 L sterile rumen fluid(SR),or 10 L saline(CON)during 3 d after *** Production performance,plasma indices,plasma lipidome,ruminal microbiome,and liver transcriptome were *** fresh and sterile RMT,we found that the molar proportion of propionic acid was increased on d 7 in the FR and SR groups and the bacterial composition was also significantly changed when compared with the CON group.A similarity analysis showed that the similarities between the CON group and FR or SR group on d 7 were 48.40%or 47.85%,whereas the similarities between microbiota on d 7 and 21 in the FR and SR groups were 68.34%or 66.85%.Dry matter intake and feed efficiency were not affected by ***β-hydroxybutyrate concentration in the FR group was decreased and significantly different lipids mainly included phosphatidylcholine and lysophosphatidylcholine containing polyunsaturated fatty *** transcriptomics analysis indicated acutephase response pathways were upregulated in the SR *** Our study suggests that RMT can shorten the transition process of the ruminal microbiota of postpartum dairy cows with no benefit on dry matter intake or feed *** with rumen fluid may not be a useful approach to promote the recovery of postpartum dairy cows.

关键词： Dairy cow Lipidomics Liver Rumen microbiota transplantation Transition period