Responses of growth performance,antioxidant function,small intestinal morphology and mRNA expression of jejunal tight junction protein to dietary iron in yellow-feathered broilers

作     者：Kaiwen Lei Hao Wu Jerry W Spears Xi Lin Xi Wang Xue Bai Yanling Huang Kaiwen Lei;Hao Wu;Jerry W Spears;Xi Lin;Xi Wang;Xue Bai;Yanling Huang

作者机构：Key Laboratory of Animal Science of State Ethnic Affairs CommissionSouthwest Minzu UniversityChengdu 610041China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and UtilizationSouthwest Minzu UniversityChengdu 610041China Department of Animal ScienceNorth Carolina State UniversityRaleighNC 27695-7621USA 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第4期

页      面：1329-1337页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(31501977) the Sichuan Provincial Key R&D Project China(22ZDYF0194) the Double World-Class Project of Southwest Minzu University China(XM2023010) 

主　　题：iron yellow-feathered broiler antioxidant function intestinal morphology tight junction protein 

摘      要：This study aimed to investigate the dose-effect of iron on growth performance,antioxidant *** morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers.A total of 7201-d-old yellow-feathered maleb roilers were allocated to 9 treatments with 8 replicate cages of 10 birds per *** dietary treatments were consisted of a basal diet(contained 79.6 mg Fe kg^(-1))supplemented with 0,20,40,60,80,160,320,640,and 1,280 mg Fe kg^(-1)in the form of FeSO_(4)·7H_(2)*** with the birds in the control group,birds supplemented with 20mg Fe kg^(-1)had higher average daily gain(ADG)(P0.0001).Adding 640 and 1,280 mg Fe kg^(-1)significantly decreased ADG(P0.0001)and average daily feed intake(ADFI)(P0.0001)compared with supplementation of 20mg Fe kg^(-1).Malondialdehyde(MDA)concentration in plasma and duodenum increased linearly(P0.0001),but MDA concentration in liver and jejunum increased linearly(P0.05)or quadratically(P0.05)with increased dietary Fe *** villus height(VH)in duodenum and jejunum,and the ratio of villus height to crypt depth(V/C)in duodenum decreased linearly(P?0.05)as dietary *** dietary Fe increased,the jejunal relative mRNA abundance of claudin-1 decreased linearly(P=0.001),but the jejunal relative mRNA abundance of zona occludens-1(ZO-1)and occludin decreased linearly(P?0.05)or quadratically(P?0.05).Compared with the supplementation of 20 mg Fe kg^(-1),the supplementation of640 mg Fe kg^(-1)or higher increased(P?0.05)MDA concentrations in plasma,duodenum,and jejunum,decreased VH in the duodenum and jejunum,and the addition of 1,280 mg Fe kg^(-1)reduced(P?0.05)the jejunal tight junction protein(claudin-1,ZO-1,occludin)mRNA *** summary,640 mg of supplemental Fe kg^(-1)or greater was associated with decreased growth performance,increased oxidative stress,disrupted intestinal morphology,and reduced mRNA expression of jejunal tight junction protein.