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Advances in Biological Chemistry 2016年第3期6卷 114-125页

作者： Masakuni Tako Rintaro Ohtoshi Kazutaka Kinjyo Shuntoku Uechi department of Subtropical Bioscience and biotechnology University of the Ryukyus Okinawa Japan Health and Longevity Research Laboratory Integrated Innovation Research Center University of the Ryukyus Okinawa Japan

The polysaccharide was isolated from Hypnea pannosa which was grown in Okinawa, Japan. The yield of the polysaccharide was 17.2%, and the total carbohydrates, pyruvic acid, sulfuric acid and ash contents were 55.2%, 3.8%, 35.2% and 24.3%, respectively. 3,6-Anhydro-α-D-galactose, β-D-galactose, α-D-galactose and D-glucose were identified by liquid and thin-layer chromatography. Fourier transform infrared (FTIR) spectra of the polysaccharide resembled that of ι-carrageenan. From the 1H- and 13C-NMR spectra, 1,3-linked β-D-galactose, 1,4-linked anhydro-α-D-galactose, 1,4-linked α-D-galactose, 1,4-linked β-D-glucose and pyruvic acid (carboxyl acetal, methyl proton and methyl carbon) were assigned. Methylation analysis revealed terminal D-galactose 0.1 mol), 1,4-linked D-glucose (1.0 mol) and 1,2,3,4,6-linked D-galactose (3.7 mol) for native polysaccharide, and terminal D-galactose, 1,4-linked D-galactose (1.9 mol), 1,4-linked D-glucose (1.0 mol), 1,3- linked D-galactose (1.7 mol), and 1,3,4,6-linked D-galactose (0.3 mol) which substituted with pyruvate group at 4 and 6 positions for desulfated polysaccharide. The polysaccharide was the novel pyruvated glucogalactan sulfate, the structure of which was proposed.

关键词： Hypnea pannosa Pyruvated Glucogalactan Sulfate 1H- and 13C-NMR Analy-sis Methylation Analysis Chemical Structure

The Interaction of Spinach Nitrite Reductase with Ferredoxin： A Site-Directed Mutation Study

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Molecular Plant 2009年第3期2卷 407-415页

作者： Masakazu Hirasawa Jatindra N. Tripathy Ramasamy Somasundaram Michael K. Johnson Megha Bhalla James R Allen David B. Knaff department of Chemistry and Biochemistry Texas Tech University Lubbock TX 79409-1061 USA department of Chemistry University of Georgia Athens GA 30602 USA department of Chemistry and Biochemistry Arizona State University Tempe AZ 85287-1604 USA Institute for biotechnology and Genomics Texas Tech University Lubbock TX 79409-1061 USA

A series of site-directed mutants of the ferredoxin-dependent spinach nitrite reductase has been characterized and several amino acids have been identified that appear to be involved in the interaction of the enzyme with ferredoxin. In a complementary study, binding constants to nitrite reductase and steady-state kinetic parameters of site-directed mutants of ferredoxin were determined in an attempt to identify ferredoxin amino acids involved in the interaction with nitrite reductase. The results have been interpreted in terms of an in-silico docking model for the 1：1 complex of ferredoxin with nitrite reductase.

关键词： Electron transport enzymology nitrogen metabolism molecular biology.

Mutually exclusive epigenetic modification on SIX6 with hypermethylation for precancerous stage and metastasis emergence tracing

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Signal Transduction and Targeted Therapy 2022年第8期7卷 2683-2686页

作者： Shihua Dong Zhicong Yang Peng Xu Wanxiang Zheng Baolong Zhang Fangqiu Fu Zhanrui Mao Jianlin Yuan Haiquan Chen Wenqiang Yu Shanghai Epiprobe biotechnology Co. LtdShanghaiChina Shanghai Public Health Clinical Center&department of General Surgery Huashan Hospital&Cancer Metastasis Institute&Institutes of Biomedical SciencesShanghai Medical CollegeFudan UniversityShanghaiChina department of Urology Xijing HospitalAir Force Medical UniversityXi’anShaanxiChina department of Thoracic Surgery Fudan University Shanghai Cancer Center&Institute of Thoracic Oncology&State Key Laboratory of Genetic EngineeringFudan UniversityShanghaiChina

Dear Editor,Aberrant DNA methylation gets involved in cancer initiation,progression,and recurrence,which in turn makes it an ideal cancer *** methylation markers or their panels have been developed in diverse cancer ***,the model-constructing based marker mining strategy and incompatibility of application have greatly impeded their ways to ***,single methylation marker applicable to all/most cancer types and multiple clinical scenarios is desperately *** hope came from the unexpected observation that HIST1H4F was universally hypermethylated in all 17 cancer types;thus,we raised the concept of“Universal Cancer Only Marker(UCOM)”and established a paradigm for discovery and clinical application of UCOM.1 Recently,a novel UCOM,hypermethylated PCDHGB7,was identified and found to advance cervical cancer(CC)screening to the precancerous stage.2 During the screening of UCOM,we discerned a bunch of cancer cell-differentially methylated regions.1 Among them,sine oculis(SIX)homeobox family of transcription factors,which were found to function as tumorigenesis regulator by promoting epithelial-to-mesenchymal transition and metastasis recently in addition to their traditional roles in tissue formation and organogenesis,3 sparked our special ***,we interrogate whether SIX6 methylation could serve as a novel UCOM and its potential applications.

关键词： metastasis precancerous raised

Phosphate Solubilization by <i>Bacillus subtilis</i>and <i>Serratia marcescens</i>Isolated from Tomato Plant Rhizosphere

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Journal of Environmental Protection 2018年第3期9卷 266-277页

作者： Eman A. H. Mohamed Azza G. Farag Sahar A. Youssef Botany and Microbiology department Faculty of Science Damanhour University Damanhour Egypt department of biotechnology Faculty of Science Taif University Taif KSA department of Virus and Phytoplasma Research Institute of Plant Pathology Research Agriculture Research Centre Cairo Egypt

Plants need phosphorus for many physiological activities in a form of phosphate anions. Three different bacterial strains (Bacillus subtilis PH, Serratia marcescens PH1, and Serratia marcescens PH2), recently isolated from tomato plant rhizosphere, have high phosphate solubilization index (SI from 2.8 to 3.2) on Pikovskaya agar medium (which contains calcium phosphate). Moreover, phosphate release from calcium in Pikovskaya broth over 5 days is increasing with cell growth for the different isolates. The most efficient phosphate solubilization case is the mixed culture of the 3 strains (OD475 is almost 1). On the other hand, pH values decreased dramatically with time due to organic acids secretion and the maximum acidification level is recoded for Serratia marcescens PH2 (pH = 1.94). Interestingly, the isolates are resistance to important pesticides (oxamyl, thiophanate methyl, and captan) that are commonly used in the sampling area. This resistance is very favorable and increases the persistence of the phosphate solubilizing bacteria in contaminated soils. The isolates are therefore plant symbionts and growth promoting agents.

关键词： Phosphate Solubilization Pikovskaya Medium Bacillus subtilis Serratia marcescens

SCALABLE AND QUANTITATIVE DECISION SUPPORT FOR THE INITIAL BUILDING DESIGN STAGES OF REFURBISHMENT

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Journal of Green Building 2019年第4期14卷 35-56页

作者： Sarah C.Andersen Kathrine L.Moller Simon W.Jorgensen Lotte B.Jensen Morten Birkved Technical University of Denmark Department of Civil EngineeringBrovej2800Kgs.LyngbyDenmark Technical University of Denmark Department of Management EngineeringProduktionstorvet2800Kgs.LyngbyDenmark Danish Technological Institute Gregersensvej2630 TaastrupDenmark A4 arkitekter og ingeniører Gladsaxevej 1042860 SoborgDenmark Hovedstadens Beredskab Bag Radhuset 31550 Copenhagen VDenmark University of Southern Denmark Department of Chemical EngineeringBiotechnology and Environmental TechnologyCampusvej 555230 Odense-MDenmark

Decision-making within the building industry often involves various criteria of very different *** are qualitative,others quantitative,some are objective,others subjective,but ultimately,they should all be aggregated and handled holistically in order to support *** process can also be referred to as multi-criteria decision-making(MCDM).Some aspects of MCDM are often conducted unconsciously and *** implementing mathematical methods that have been proved applicable for MCDM,multi-criteria decision-making processes can be handled more consciously and transparently and thus be made *** calculation method presented allows quantitative sustainability and qualitative indicator values to be accounted for with the level of importance *** MCDM method used is Technique for Order of Preference by Similarity to Ideal Solution(TOPSIS).As the model has already been described well in the scientific literature and is used frequently,the aim is to illustrate how TOPSIS can be applied for transparent decision-making within the building industry in the context of urban renewal and refurbishment schemes through case studies of various scales and *** case studies focused on in this paper incorporate a variety of specific pre-chosen criteria,including environmental performance,functional parameters and technical *** case studies cover different parameters of refurbishment in a major hospital compound in Denmark due to be taken out of *** central design decision is whether to refurbish or demolish the old hospital *** results reveal that decision support is first of all dependent on how the decision-making tool is applied and what choices are made in relation to the actual ***,by implementing a mathematically based MCDM method like that being assessed in the case studies presented,the decisions and their argu-ments become transparent and are easily communicated withi

关键词： Multi-Criteria Decision Making(MCDM) mathematical decision support building design transparent decision making refurbishment urban renewal

Induction of clusterin Expression by Neuronal Cell Death in Zebrafish

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Journal of Genetics and Genomics 2014年第11期41卷 583-589页

作者： Yun-Mi Jeong Tae-Eun Jin Jung-Hwa Choi Mi-Sun Lee Hyun-Taek Kim Kyu-Seok Hwang Doo-Sang Park Hyun-Woo Oh Joong-Kook Choi Vladimir Korzh Melitta Schachner Kwan-Hee You Cheol-Hee Kim department of Biology Chungnam National University Korea Research Institute of Bioscience and biotechnology department of Biochemistry College of MedicineChungbuk National University Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research Keck Center for Collaborative Neuroscience and department of Cell Biology and Neuroscience Rutgers University Center for Neuroscience Shantou University Medical College

Clusterin, a protein associated with multiple functions, is expressed in a wide variety of mammalian tissues. Although clusterin is known to be involved in neurodegenerative diseases, ageing, and tumorigenesis, a detailed analysis of the consequences of gain- or loss-of- function approaches has yet to be performed to understand the underlying mechanisms of clusterin functions. Since clusterin levels change in neurological diseases, it is likely that clusterin contributes to cell death and degeneration in general. Zebrafish was investigated as a model system to study human diseases. During development, zebrafish clusterin was expressed in the notochord and nervous system. Embryonic overexpression of clusterin by mRNA microinjection did not affect axis formation, whereas its knock-down by anti-sense morpholino treatment resulted in neuronal cell death. To analyze the function of clusterin in neurodegeneration, a transgenic zebrafish was investigated, in which nitroreductase expression is regulated under the control of a neuron-specifc huC promoter which is active between the stages of early neuronal precursors and mature neurons. Nitroreductase turns metronidazole into a cytotoxic agent that induces cell death within 12 h. After metronidazole treatment, transgenic zebrafish showed neuron-specific cell death. Interestingly, we also observed a dramatic induction of clusterin expression in the brain and spinal cord in these fish, suggesting a direct or indirect role of clusterin in neuronal cell death and thus, more generally, in neurodegeneration.

关键词： Clusterin Neuronal cell death Neurodegeneration Zebrafish

Modeling Solid Waste Minimization Performance at Source in Dar es Salaam City, Tanzania

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Journal of Geoscience and Environment Protection 2024年第9期12卷 17-32页

作者： Abdon Salim Mapunda Richard Joseph Kimwaga Shaaban Ally Kassuwi department of Integrated Water Resources Engineering University of Dar es Salaam Dar es Sa-laam Tanzania department of Molecular Biology and biotechnology University of Dar es Salaam Dar es Salaam Tanzania

Municipal solid waste generation is strongly linked to rising human population and expanding urban areas, with significant implications on urban metabolism as well as space and place values redefinition. Effective management performance of municipal solid waste management underscores the interdisciplinarity strategies. Such knowledge and skills are paramount to uncover the sources of waste generation as well as means of waste storage, collection, recycling, transportation, handling/treatment, disposal, and monitoring. This study was conducted in Dar es Salaam city. Driven by the curiosity model of the solid waste minimization performance at source, study data was collected using focus group discussion techniques to ward-level local government officers, which was triangulated with literature and documentary review. The main themes of the FGD were situational factors (SFA) and local government by-laws (LGBY). In the FGD session, sub-themes of SFA tricked to understand how MSW minimization is related to the presence and effect of services such as land use planning, availability of landfills, solid waste transfer stations, material recovery facilities, incinerators, solid waste collection bins, solid waste trucks, solid waste management budget and solid waste collection agents. Similarly, FGD on LGBY was extended by sub-themes such as contents of the by-law, community awareness of the by-law, and by-law enforcement mechanisms. While data preparation applied an analytical hierarchy process, data analysis applied an ordinary least square (OLS) regression model for sub-criteria that explain SFA and LGBY;and OLS standard residues as variables into geographically weighted regression with a resolution of 241 × 241 meter in ArcMap v10.5. Results showed that situational factors and local government by-laws have a strong relationship with the rate of minimizing solid waste dumping in water bodies (local R square = 0.94).

关键词： Modeling Solid Waste Minimization Dar es Salaam City Ordinary Least Square (OLS) Regression Model Situation Factors Local Government by Laws

Safety evaluation in mice of the childhood immunization vaccines from two south-eastern states of Nigeria

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Asian Pacific Journal of Tropical Biomedicine 2015年第2期5卷 132-137页

作者： Oli Angus Nnamdi Agu Remigus Uchenna Oli Ugochukwu Chinedum Nwoye Charles Ugochukwu Ejiofor Obiora Shedrack Esimone Charles Okechukwu department of Pharmaceutical Microbiology and biotechnology Faculty of Pharmaceutical SciencesNnamdi Azikiwe University Biopharmaceutics and Drug Delivery Laboratory College of Pharmacy5968 College Street.PO Box 15000Dalhousie University departments of Chemical Pathology Nnamdi Azikiwe UniversityTeaching Hospital departments of Hematology Nnamdi Azikiwe UniversityTeaching Hospital department of Pediatrics Anambra State University Teaching Hospital

Objective:To check the effects of the vaccines on the hematopoietic system and weight of mice after ***:The study was done with the Expanded Programme on Immunization vaccines donated by the Ministries of Health of Abia and Imo States of *** vaccines were collected from the cold-chain stores and transported in vaccine carriers to the cold-chain facility in Nnamdi Azikiwe University Teaching Hospital within 3 hours of *** were used to immunize a total of 160 *** Ethics Committee of Nnamdi Azikiwe University Teaching Hospital,Nnewi of Anambra State,Nigeria approved the ***:Mice body weight changes test showed that the mice all had increased body weight at Days 3 and 7 post-immunization and none died during the 7 d post-immunization *** percentage weight gains of the mice compared with the control were 69%.70%,64%.63%,65%and 68%for oral polio vaccine,*** CalmetteGuerin,measles,yellow fever and hepatitis B vaccines respectively collected from Imo *** mice immunized with oral polio vaccine,*** ***,yellow fever and hepatitis B vaccines collected from Abia State had 123%.114%,121%.116%,142%and 119%weight gain respectively compared with the *** promoting toxicity test showed that none of the vaccines was able to induce proliferation of leukocytes up to ten *** toxicity test showed that all the vaccines had an leukopenic toxicity test value higher than 80%of the control(physiological saline).Conclusions:The vaccine samples tested were safe and did not affect the hematopoietic system *** storage conditions of the vaccines in the States' cold-chain stores had not compromised the safety of the vaccines.

关键词： Safety evaluation Vaccines Routine immunization Imo and Abia Nigeria

Multi-omics characterization and therapeutic liability of ferroptosis in melanoma

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Signal Transduction and Targeted Therapy 2022年第9期7卷 3194-3197页

作者： Yi He Yu Dong Yong Chen Guanxiong Zhang Hailun Zhang Guang Lei Yanhua Du Xiang Chen Youqiong Ye Hong Liu department of Dermatology Hunan Key Laboratory of Skin Cancer and PsoriasisHunan Engineering Research Center of Skin Health and DiseaseXiangya Clinical Research Center for Cancer ImmunotherapyXiangya HospitalCentral South UniversityChangshaChina Shanghai Institute of Immunology Department of Immunology and MicrobiologyShanghai Jiao Tong University School of MedicineShanghaiChina department of Musculoskeletal Surgery Fudan University Shanghai Cancer CenterShanghaiPR China department of Oncology Shanghai Medical CollegeFudan UniversityShanghaiPR China department of Research and Development Beijing GAP Biotechnology Co.LtdBejingChina department of Radiation Oncology Hunan Cancer Hospital and The Affliated Cancer Hospital of Xiangya School of MedicineCentral South UniversityChangshaChina

Dear Editor,Ferroptosis,an iron-dependent form of programmed cell deathdriven by excessive lipid peroxidation,1has emerged as,apromising andeffective strategy fortrigering cancer celldeath.2,3Insufficient understanding of the role of ferroptosis in melanomaprogression and the tumor microenvironment(TME)has limitedthe development of ferroptosis-targeted therapy and combina-tions with other therapeutic strategies.

关键词： therapeutic melanoma peroxidation