The Interaction of Spinach Nitrite Reductase with Ferredoxin： A Site-Directed Mutation Study
The Interaction of Spinach Nitrite Reductase with Ferredoxin： A Site-Directed Mutation Study作者机构：Department of Chemistry and Biochemistry Texas Tech University Lubbock TX 79409-1061 USA Department of Chemistry University of Georgia Athens GA 30602 USA Department of Chemistry and Biochemistry Arizona State University Tempe AZ 85287-1604 USA Institute for Biotechnology and Genomics Texas Tech University Lubbock TX 79409-1061 USA
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2009年第2卷第3期
页 面：407-415页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0902[农学-园艺学] 090102[农学-作物遗传育种] 090202[农学-蔬菜学]
基 金：National Institutes of Health, NIH U.S. Department of Energy, USDOE: DE-FG03-99ER20346 National Institute of General Medical Sciences, NIGMS: R01GM062524 Office of Science, SC Chemical Sciences, Geosciences, and Biosciences Division, CSGB
主 题：Electron transport enzymology nitrogen metabolism molecular biology.
摘 要：A series of site-directed mutants of the ferredoxin-dependent spinach nitrite reductase has been characterized and several amino acids have been identified that appear to be involved in the interaction of the enzyme with ferredoxin. In a complementary study, binding constants to nitrite reductase and steady-state kinetic parameters of site-directed mutants of ferredoxin were determined in an attempt to identify ferredoxin amino acids involved in the interaction with nitrite reductase. The results have been interpreted in terms of an in-silico docking model for the 1：1 complex of ferredoxin with nitrite reductase.
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