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Nano-Micro Letters 2010年第3期2卷 160-163页

作者： S.Karthick Raja Namasivayam Gnanendra Kumar E Reepika R department of biotechnology Sathyabama University

Silver nanoparticles synthesized by dried biomass of Lactobaciluus acidophilus 01 strain was evaluated against toxicity of genomic DNA isolated from bacteria(***) fungi(Beauveria bassiana) algae(Seenedesmus acutus) and human blood adopting standard condition was discussed in the present *** silver nanoparticles were characterized by UV-Vis spectroscopy and *** UV-Vis spectroscopy revealed the formation of silver nanoparticles by yielding the typical silver plasmon absorption maxima at 430 nm and SEM micrograph indicates the uniform spherical particles within the size range of 45?60 *** energy dispersive X-ray spectroscopy(EDX) of the nanoparticle confirmed the presence of elemental silver signal as strong *** above synthesized silver nanoparticles didn't cause any toxic effect on all the tested genomic DNA at all tested concentrations which reveals nil genomic nanoparticles induced toxicity.

关键词： Lactobacillus acidophilus Silver nanoparticles Antibacterial activity Biosynthesis

Embryo culture is an efficient way to conserve a medicinally important endangered forest tree species Strychnos potatorum

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Journal of Forestry Research 2013年第2期24卷 279-283页

作者： Srikanth Kagithoju Vikram Godishala Madhusudhan Kairamkonda Rama Swamy Nanna department of biotechnology Kakatiya University

The present study reports a protocol for germination of Strychnos potatorum （ver. Tel. Chilla） using zygotic embryo culture as an embryo rescue method. A 100% germination rate was obtained by culturing the embryos on full-strength Murashige and Skoog＇s medium （MS） containing 20 g/L sucrose in comparison to McCown and Lloyd＇s Woody Plant Medium （WPM）. Germination rates decreased when the sucrose concentration was lower or higher than 20 g·L-1 . WPM/MS medium containing glucose at levels 30, 20, 15 g·L-1 showed a smaller percentage of germination and at quarter strength, WPM/MS medium with glucose did not respond. Multiple shoot formation was found at 1.0 2.0 mg/L BAP; 3.0 mg/L Kn; 2.0 mg/L TDZ on MS medium with 20 g·L-1 sucrose. Germination rates improved when the embryos were placed upright （vertically） in the medium. The in vitro germinated seedlings were acclimatized in a walk-in-chamber and maintained in the green house with the survival rate of 65% 75%. These plants were transferred to the field and were found to be phenotypically normal, healthy and similar to donor plants. This protocol will be useful to overcome seed dormancy and for rapid multiplication and conservation of S. potatorum using zygotic embryo culture.

关键词： endangered tree species Strychnos potatorum In vitro zygotic embryo culture immobilization of zygotic embryos embryo rescue

Photocatalytic degradation of endocrine disruptor Bisphenol-A in the presence of prepared Ce_xZn_(1-x)O nanocomposites under irradiation of sunlight

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Journal of Environmental Sciences 2014年第11期26卷 2362-2368页

作者： Kamaraj M. Ranjith K.S. Rajeshwari Sivaraj Rajendra Kumar R.T. Hasna Abdul Salam department of biotechnology Dr.N.G.P. Arts and Science College Coimbatore 641048 Tamil Nadu India Advanced materials and Devices Laboratory Department of Physics Bharathiar University Coimbatore 641046 Tamil Nadu India department of biotechnology School of Life Sciences Karpagam University Coimbatore 641021 Tamil Nadu India

Photocatalytic degradation of Bisphenol A （BPA）, a representative endocrine disruptor chemical, was carried out under irradiation of sunlight in the presence of CexZn1-xO nanophotocatalyst. Cerium （Ce） ions were successfully incorporated into the bulk lattice of ZnO by simple co-precipitation process. The CexZn1-xO composite nanostructures exhibited higher photocatalytic efficiency than pure ZnO in the degradation of BPA under sunlight irradiation and nearly complete mineralization of BPA was achieved. The degradation rate was strongly dependent on factors such as the size and structure of catalyst, doping material concentration, BPA concentration, catalyst load, irradiation time and pH levels. This work suggested that the CexZn1-xO assisted photocatalytic degradation is a versatile, economic, environmentally benign and efficient method for BPA removal in the aqueous environment.

关键词： Bisphenol A (BPA) ZnO CexZn1-xO Sunlight Co-precipitation Cerium

Remediation of Fly Ash Dumpsites Through Bioenergy Crop Plantation and Generation: A Review

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Pedosphere 2018年第4期28卷 561-580页

作者： Madhumita ROY Roopali ROYCHOWDHURY Pritam MUKHERJEE department of biotechnology Techno India University

Utilization of fly ash, a byproduct of coal combustion in thermal power plants, is a sustainable use of waste for power *** fly ash as waste in landfills/ash ponds may not only be regarded as a loss of valuable land and essential nutrients, but also pose a significant health hazard due to fine air-borne particles and leaching of heavy metals. The presence of essential macro-and micronutrients and its porosity make fly ash an excellent soil amendment for plant growth as an organic nitrogen(N) and carbon(C)supplementation. As harmful heavy metals make fly ash unsafe for agronomy, bioenergy crop plantation and energy generation from different thermochemical conversions of the biomass would be an ideal method for coal fly ash utilization through which carbon-neutral fuel can be generated from fossil fuel, thus reducing climate change impact. This review summarizes the development of bioenergy plantation and silviculture at fly ash dumpsites with an integrated phyto-bio-rhizo-mycoremediation approach and assesses utilization of the valuable biomass for thermal energy, electricity, and biofuel generation with inclusion of a SWOT analysis(a strategic technique typically used to help identify the strength, weakness, opportunities, and threat). Bioenergy crop production through integrated phytomanagement can generate billions of dollars of wealth from waste and provides a sustainable solution for fly ash management,with environmental, economic, and social benefits.

关键词： biofuel production biomass-generating crops carbon-neutral fuel health risk heavy metal land use phytoremediation soil amendment

Food components and diet habits:chief factors of cancer development

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Food Quality and Safety 2019年第4期3卷 227-231页

作者： Rihab Ksouri department of biotechnology Biotechnology InstituteAnkara UniversityTurkey

Food is a vital need for ***,there is food for all,but the world still suffers from under-and over-nutrition and risk of cancer development and chronic diseases can follow both ***,cancer is a leading cause of mortality after cardiovascular disease;it is considered the second reason for death *** of nutritional habits,the quality of food,the consumption of canned foods,genetically modified fruits and vegetables and exposed food to certain pesticides and carcinogens agents,and unhealthy lifestyle behaviours such as smoking,alcohol,obesity,and fast-foods consumption may be at risk to the development of some *** recent decades,researchers have carried out attention in this field to improve the quality of life and to limit nutrition ***,this study aims to summarize current evidence on the relationship between nutritional factors and cancer expansion,how nutrition can be a heal and a source of fatal illness leading to *** detail,this review will highlight the influence of specific foodstuffs on the threat of cancer incidence and recurrence by providing some examples of most carcinogenic compounds.

关键词： cancer carcinogens food nutritional habits

Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies:Characterization and stability studies

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Chinese Journal of Chemical Engineering 2019年第1期27卷 191-199页

作者： Meisam Sharifi Seyed-Mortaza Robatjazi Minoo Sadri Jafar Mohammadian Mosaabadi department of Bioscience and biotechnology Malek Ashtar University of Technology

The plant cellulose powder was activated by two different methods using 1,4-butanediol diglycidyl ether(BTDE)and 1,1′-Carbonyldiimidazole(CDI) as the chemical coupling *** hydrolase(OPH) from Flavobacterium ATCC 27551 was immobilized on any of activated support through covalent *** optimal conditions of affecting parameters on enzyme immobilization in both methods were found, and it was demonstrated that the highest activity yields of immobilized OPH onto epoxy and CDI treated cellulose were 68.32%and 73.51%, *** surface treatment of cellulose via covalent coupling with BTDE and CDI agents was proved by FTIR *** kinetic constants of the free and immobilized enzymes were determined, and it was showed that both immobilization techniques moderately increased the Kmvalue of the free *** improvements in storage and thermal stability were investigated and depicted that the half-life of immobilized OPH over the surface of epoxy modified cellulose had a better growth compared to the free and immobilized enzymes onto CDI treated ***, the pH stability of the immobilized preparations was enhanced relative to the free counterpart and revealed that all enzyme samples would have the same optimum pH value for stability at ***, the immobilized OPH onto epoxy and CDI activated cellulose retained about 59% and 68% of their initial activity after ten turns of batch operation, *** results demonstrated the high performance of OPH enzyme in immobilized state onto an inexpensive support with the potential of industrial applications.

关键词： Organophosphorus hydrolase Cellulose powder Activation Immobilization Stability

Cloning and Characterization of an mRNA Encoding F_1-ATPase Beta-Subunit Abundant in Epithelial Cells of Mantle and Gill of Pearl Oyster, Pinctada fucata

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Tsinghua Science and Technology 2007年第4期12卷 381-388页

作者：刘亮谢莉萍熊训浩范为民陈蕾张荣庆 Institute of Marine biotechnology Department of Biological Sciences and Biotechnology Tsinghua University

In oyster biomineralization, large amounts of calcium are absorbed from external media, transported to the mineralization site, and finally deposited via a matrix-mediated process, All these activities are very energy intensive; therefore, investigations of the energy metabolism pathways of different oyster tissues will facilitate understanding of oyster biomineralization physiology. A full-length cDNA encoding the F1- ATPase beta-subunit （the F1-β-subunit, a major calalytic subunit of F-ATPase） from the pearl oyster （Pinctada fucata） was cloned using the homology strategy with a pair of degenerated primers based on the conserved regions of other animals＇ F1-β-subunit genes. Sequencing and structural analyses showed that the obtained sequence shared high identity with other animals＇ F1-β-subunits, and had a unique phosphorylation site of PKC and CK II on the external surface of the putative protein. Results from semi-quantitative reverse transcription-polymerase chain reaction and in situ hybridization demonstrated this oyster F1-β-subunit mRNA is abundant in the gill and mantle, and distributed widely in the periostracal groove, the outer folder. and the dorsal region of the mantle and in the gill epithelial cells. These tissues were the main regions that participate in biomineralization processes such as calcium uptake, transport, and matrix secretion. The results indicate that tissues involved in biomineralization have stronger energy metabolic processes and that F1-ATPase might play an important role in oyster biomineralization by providing energy transport.

关键词： biomineralization F1-β-subunit mantle gill Pinctada fucata

An Effective Integrated Machine Learning Framework for Identifying Severity of Tomato Yellow Leaf Curl Virus and Their Experimental Validation

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Research 2023年第3期2023卷 91-104页

作者： Nattanong Bupi Vinoth Kumar Sangaraju Le Thi Phan Aamir Lal Thuy Thi Bich Vo Phuong Thi Ho Muhammad Amir Qureshi Marjia Tabassum Sukchan Lee Balachandran Manavalan department of Integrative biotechnology College of Biotechnology and BioengineeringSungkyunkwan UniversitySuwon 16419.Gyeonggi-doRepublic of Korea Computational Biology and Bioinformatics Laboratory Department of Integrative BiotechnologyCollege of Biotechnology and Bioengineering.Sungkyunkwan UniversitySuwon 16419Gyeonggi-doRepublic of Korea

Tomato yellow leaf curl virus(TYLCV)dispersed across different countries,specifically to subtropical regions,associated with more severe *** TYLCV was first isolated in 1931,it has been a menace to tomato industrial production worldwide over the past *** groups were newly isolated from TYLCV-resistant tomatoes in 2022;however,their functions are *** development of machine learning(ML)-based models using characterized sequences and evaluating blind predictions is one of the major challenges in interdisciplinary *** purpose of this study was to develop an integrated computational framework for the accurate identification of symptoms(mild or severe)based on TYLCV sequences(isolated in Korea).For the development of the framework,we first extracted 11 different feature encodings and hybrid features from the training data and then explored 8 different classifiers and developed their respective prediction models by using randomized 10-fold ***,we carried out a systematic evaluation of these 96 developed models and selected the top 90 models,whose predicted class labels were combined and considered as reduced *** the basis of these features,a multilayer perceptron was applied and developed the final prediction model(IML-TYLCVs).We conducted blind prediction on 3 groups using IML-TYLCVs,and the results indicated that 2 groups were severe and 1 group was ***,we confirmed the prediction with virus-challenging experiments of tomato plant phenotypes using infectious clones from 3 *** virologists and plant breeding professionals can access the user-friendly online IML-TYLCVs web server at https://***/IML-TYLCVs,which can guide them in developing new protection strategies for newly emerging viruses.

关键词： breeding fir prediction

Glioma cell line proliferation controlled by different chemical functional groups in vitro

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Frontiers of materials Science 2013年第1期7卷 69-75页

作者： Su-Ju XU Fu-Zhai CUI Xiao-Long YU Xiang-Dong KONG Xinyuan Institute of Medicine and biotechnology School of Life Science Zhejiang Sci-Tech University Hangzhou 310018 China Institute of Regenerative Medicine anclBiomimetic materials Department of Materials Science and Engineering Tsinghua University Beijing 100084 China

Glioma cell line C6 cultured on silicon surfaces modified by different chemical functional groups, including mercapto (-SH), carboxyl (-COOH), amino (-NH2), hydroxyl (-OH) and methyl (-CH3) groups, was studied here to investigate the influence of surface chemistry on the cell proliferation, adhesion and apoptosis. AFM confirmed the similar characteristic of different functional groups occupation. The adhering C6 exhibited morphological changes in response to different chemical functional groups. The C6 adhered to -COOH, -NH2, -OH and -CH3 surfaces and flattened morphology, while those on -SH surface exhibited the smallest contact area with mostly rounded morphology, which led to the death of cancer cells. The results of MTT assay showed that the -COOH and -NH2 groups promoted cell proliferation, while the -SH significantly inhibited the proliferation. Compared with other chemical functional groups, the -SH group exhibited its unique effect on the fate of cancer cells, which might provide means for the design of biomaterials to prevent and treat glioma.

关键词： glioma cell line C6 chemical functional group adhesion proliferation

Helicobacter pylori antibiotic resistance in Iran

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World Journal of Gastroenterology 2005年第38期11卷 6009-6013页

作者： Marjan Mohammadi Delaram Doroud Nazanin Mohajerani Sadegh Massarrat biotechnology Research Center Gastroenterology department

AIM： To examine the frequency of antibiotic resistance in Iranian Helicobacter pylori （ H pylon） strains isolated from two major hospitals in Tehran.METHODS： Examination of antibiotic resistance was performed on 120 strains by modified disc diffusion test and PCR-RFLP methods. In addition, in order to identify the possible causes of the therapeutic failure in Iran, we also determined the resistance of these strains to the most commonly used antibiotics （metronidazole, amoxicillin, and tetracycline） by modified disc diffusion test. RESULTS： According to modified disc diffusion test, 1.6% of the studied strains were resistant to amoxicillin, 16.7% to clarithromycin, 57.5% to metronidazole, and there was no resistance to tetracycline. Of the clarithromycin resistant strains, 73.68% had the A2143G mutation in the 23SrRlVA gene, 21.05% A2142C, and 5.26% A2142G. None of the sensitive strains were positive for any of the three point mutations. Of the metronidazole resistant strains, deletion in rdxA gene was studied and detected in only 6 （5%） of the antibiogram-based resistant strains. None of the metronidazole sensitive strains possessed rdxA gene deletion. CONCLUSION： These data show that despite the fact that clarithromycin has not yet been introduced to the Iranian drug market as a generic drug, nearly 20% rate of resistance alerts toward the frequency of macrolide resistance strains, which may be due to the widespread prescription of erythromycin in Iran. rdxA gene inactivation, if present in Iranian Hpyloristrains, may be due to other genetic defects rather than gene deletion.

关键词： H pylori Clarithromycin Metronidazole Resistance Iran