检索结果-南通市图书馆

Induced pluripotent stem cells(iPSCs):molecular mechanisms of induction and applications

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2024年第5期9卷 2065-2090页

作者： Jonas Cerneckis Hongxia Cai Yanhong Shi department of Neurodegenerative Diseases Beckman Research Institute of City of HopeDuarteCA 91010USA Irell&Manella Graduate School of Biological sciences Beckman Research Institute of City of HopeDuarteCA 91010USA

The induced pluripotent stem cell(iPSC)technology has transformed in vitro research and holds great promise to advance regenerative *** have the capacity for an almost unlimited expansion,are amenable to genetic engineering,and can be differentiated into most somatic cell *** have been widely applied to model human development and diseases,perform drug screening,and develop cell *** this review,we outline key developments in the iPSC field and highlight the immense versatility of the iPSC technology for in vitro modeling and therapeutic *** begin by discussing the pivotal discoveries that revealed the potential of a somatic cell nucleus for reprogramming and led to successful generation of *** consider the molecular mechanisms and dynamics of somatic cell reprogramming as well as the numerous methods available to induce ***,we discuss various iPSC-based cellular models,from mono-cultures of a single cell type to complex three-dimensional organoids,and how these models can be applied to elucidate the mechanisms of human development and *** use examples of neurological disorders,coronavirus disease 2019(COVID-19),and cancer to highlight the diversity of disease-specific phenotypes that can be modeled using iPSC-derived *** also consider how iPSC-derived cellular models can be used in high-throughput drug screening and drug toxicity ***,we discuss the process of developing autologous and allogeneic iPSC-based cell therapies and their potential to alleviate human diseases.

关键词： mechanisms programming holds

Transcriptomic and Macroscopic Architectures of Multimodal Covariance Network Reveal Molecular–Structural–Functional Co-alterations

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research 2024年第1期2023卷 471-487页

作者： Lin Jiang Yueheng Peng Runyang He Qingqing Yang Chanlin Yi Yuqin Li Bin Zhu Yajing Si Tao Zhang Bharat B.Biswal Dezhong Yao Lan Xiong Fali Li Peng Xu The Clinical Hospital of Chengdu Brain Science Institute MOE Key Lab for NeuroinformationUniversity of Electronic Science and Technology of ChinaChengdu 611731China School of Life Science and Technology Center for Information in BioMedicineUniversity of Electronic Science and Technology of ChinaChengdu 611731China School of Psychology Xinxiang Medical UniversityXinxiang 453003China School of Science Xihua UniversityChengdu 610039China department of Biomedical Engineering New Jersey Institute of TechnologyNewarkNJUSA School of Electrical Engineering Zhengzhou UniversityZhengzhou 450001China Research Unit of NeuroInformation Chinese Academy of Medical Sciences2019RU035ChengduChina Montreal neurological Institute and Hospital McGill UniversityMontrealQCCanada department of Electrical and Computer Engineering Faculty of Science and TechnologyUniversity of MacaoMacaoChina Radiation Oncology Key Laboratory of Sichuan Province 610041 ChengduChina Rehabilitation Center Qilu Hospital of Shandong UniversityJinan 250012China

Human cognition is usually underpinned by intrinsic structure and functional neural co-activation in spatially distributed brain *** to lacking an effective approach to quantifying the covarying of structure and functional responses,how the structural–functional circuits interact and how genes encode the relationships,to deepen our knowledge of human cognition and disease,are still ***,we propose a multimodal covariance network(MCN)construction approach to capture interregional covarying of the structural skeleton and transient functional activities for a single *** further explored the potential association between brain-wide gene expression patterns and structural–functional covarying in individuals involved in a gambling task and individuals with major depression disorder(MDD),adopting multimodal data from a publicly available human brain transcriptomic atlas and 2 independent *** analysis showed a replicable cortical structural–functional fine map in healthy individuals,and the expression of cognition-and disease phenotype-related genes was found to be spatially correlated with the corresponding MCN *** analysis of cell type-specific signature genes suggests that the excitatory and inhibitory neuron transcriptomic changes could account for most of the observed correlation with task-evoked MCN differences.

关键词： alterations evoked spatially

Association of TRMT2B gene variants with juvenile amyotrophic lateral sclerosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2024年第1期18卷 68-80页

作者： Yanling Liu Xi He Yanchun Yuan Bin Li Zhen Liu Wanzhen Li Kaixuan Li Shuo Tan Quan Zhu Zhengyan Tang Feng Han Ziqiang Wu Lu Shen Hong Jiang Beisha Tang Jian Qiu Zhengmao Hu Junling Wang department of Neurology Xiangya HospitalCentral South UniversityJiangxiNational Regional Center for Neurological DiseasesNanchang330038China Provincial Laboratory for Diagnosis and Treatment of Genitourinary System Disease Department of UrologyXiangya HospitalCentral South UniversityChangsha410078China department of Orthopedics The First Affiliated HospitalZhejiang University School of MedicineHangzhou310002China department of Neurology Xiangya HospitalCentral South UniversityChangsha410078China National Clinical Research Center for Geriatric Diseases Xiangya HospitalCentral South UniversityChangsha410008China Key Laboratory of Hunan Province in Neurodegenerative Disorders Central South UniversityChangsha410008China Center for Medical Genetics School of Life SciencesCentral South UniversityChangsha410008China Engineering Research Center of Hunan Province in Cognitive Impairment Disorders Central South UniversityChangsha410078China Hunan International Scientific and Technological Cooperation Base of Neurodegenerative and Neurogenetic Diseases Changsha410078China Hunan Key Laboratory of Molecular Precision Medicine Xiangya HospitalCentral South UniversityChangsha410078China

Amyotrophic lateral sclerosis(ALS)is a fatal neurodegenerative disease characterized by progressive degeneration of motor neurons,and it demonstrates high clinical heterogeneity and complex genetic architecture.A variation within TRMT2B(c.1356G>T;p.K452N)was identified to be associated with ALS in a family comprising two patients with juvenile ALS(JALS).Two missense variations and one splicing variation were identified in 10 patients with ALS in a cohort with 910 patients with ALS,and three more variants were identified in a public ALS database including 3317 patients with ALS.A decreased number of mitochondria,swollen mitochondria,lower expression of ND1,decreased mitochondrial complex I activities,lower mitochondrial aerobic respiration,and a high level of ROS were observed functionally in patient-originated lymphoblastoid cell lines and TRMT2B interfering HEK293 ***,TRMT2B variations overexpression cells also displayed decreased *** conclusion,a novel JALS-associated gene called TRMT2B was identified,thus broadening the clinical and genetic spectrum of ALS.

关键词： TRMT2B amyotrophic lateral sclerosis mitochondrial complex I tRNA methylation reactive oxygen species

Chinese herbal medicine Ginkgo biloba *** for ischemic stroke:An overview of systematic reviews and meta-analyses

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Medicine 2024年第2期22卷 163-179页

作者： Tian-tian Meng Ya-ping You Min Li Jian-bo Guo Xin-bin Song Jing-yi Ding Xiao-long Xie An-qi Li Shang-jin Li Xiang-jun Yin Peng Wang Zhe Wang Bao-liang Wang Qing-yong He department of Cardiology Guang'anmen HospitalChina Academy of Chinese Medical SciencesBeijing 100032China department of Rehabilitation Dongfang HospitalBeijing University of Chinese MedicineBeijing 100071China School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Chinese Medicine the University of Hong KongHong KongChina Graduate School Henan University of Chinese MedicineZhengzhou 450008Henan ProvinceChina department of Acupuncture and Moxibustion Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100010China department of Cardiology Guang’anmen HospitalChina Academy of Chinese Medical SciencesZhengzhou 450008Henan ProvinceChina School of Integrated Traditional Chinese and Western Medicine Southwest Medical UniversityHangzhou 310053Zhejiang ProvinceChina School of Basic Medical sciences Zhejiang Chinese Medical UniversityHangzhu 310053Zhejang ProvinceChina department of Neurology the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 450003Henan ProvinceChina

Background:Ginkgo biloba ***(GBLPs)are a class of Chinese herbal medicine used in the adjuvant treatment of ischemic stroke(IS).Recently,several systematic reviews(SRs)and meta-analyses(MAs)of GBLPs for IS have been ***:This overview aims to assess the quality of related SRs and *** strategy:Pub Med,Embase,Cochrane Library,Web of Science,Chinese Biological Medicine,China National Knowledge Infrastructure,Wanfang,and Chinese Science and Technology Journals databases were searched from their inception to December 31,*** criteria:SRs and MAs of randomized controlled trials(RCTs)that explored the efficacy of GBLPs for patients with IS were *** extraction and analysis:Two independent reviewers extracted data and assessed the methodological quality,risk of bias(ROB),reporting quality,and credibility of evidence of the included SRs and MAs using A Measurement Tool to Assess Systematic Reviews 2(AMSTAR 2),Risk of Bias in Systematic Reviews(ROBIS),the Preferred Reporting Items for Systematic Reviews and Meta-analyses(PRISMA),and the Grading of Recommendations Assessment,Development and Evaluation(GRADE),***,descriptive analysis and data synthesis were ***:Twenty-nine SRs/MAs involving 119 outcomes were included in this *** overall methodological quality of all SRs/MAs was critically low based on AMSTAR 2,and 28 had a high ROB based on the *** to the PRISMA statement,the reporting items of the included SRs/MAs are relatively *** results based on GRADE showed that of the 119 outcomes,8 were rated as moderate quality,24 as low quality,and 87 as very low *** on the data synthesis,GBLPs used in conjunction with conventional treatment were superior to conventional treatment alone for decreasing neurological function ***:GBLPs can be considered a beneficial supplemental therapy for ***,because of the low quality of the existing eviden

关键词： statement synthesis alone

Epidemiological and clinical features,treatment status,and economic burden of traumatic spinal cord injury in China:a hospital-based retrospective study

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第5期19卷 1126-1132页

作者： Hengxing Zhou Yongfu Lou Lingxiao Chen Yi Kang Lu Liu Zhiwei Cai David BAnderson Wei Wang Chi Zhang Jinghua Wang Guangzhi Ning Yanzheng Gao Baorong He Wenyuan Ding Yisheng Wang Wei Mei Yueming Song Yue Zhou Maosheng Xia Huan Wang Jie Zhao Guoyong Yin Tao Zhang Feng Jing Rusen Zhu Bin Meng Li Duan Zhongmin Zhang Desheng Wu Zhengdong Cai Lin Huang Zhanhai Yin Kainan Li Shibao Lu Shiqing Feng department of Orthopedics The Second HospitalCheeloo College of MedicineShandong UniversityShandongChina department of Orthopedics Shandong University Center for OrthopedicsQilu HospitalCheeloo College of MedicineShandong UniversityShandongChina department of Orthopedics Tianjin Medical University General HospitalInternational Science and Technology Cooperation Base of Spinal Cord InjuryTianjin Key Laboratory of Spine and Spinal CordTianjinChina Faculty of Medicine and Health The Back Pain Research TeamSydney Musculoskeletal HealthThe Kolling InstituteUniversity of SydneySydneyNSWAustralia department of Spine Surgery Honghui HospitalXi’an Jiaotong UniversityXi’anShaanxi ProvinceChina department of Burns and Plastic Surgery The Fourth Medical Center of Chinese PLA General HospitalBeijingChina Faculty of Medicine and Health School of Health SciencesUniversity of SydneyNSWAustralia Sydney Spine Institute BurwoodSydneyNSWAustralia Laboratory of Epidemiology Tianjin Neurological InstituteDepartment of NeurologyTianjin Medical University General Hospital&Tianjin Neurological InstituteKey Laboratory of Post-Neuroinjury Neuro-repair and Regeneration in Central Nervous SystemMinistry of Education and Tianjin CityTianjinChina department of Orthopedics Henan Provincial People’s HospitalZhengzhouHenan ProvinceChina department of Spinal Surgery The Third Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina department of Orthopedics The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina department of Spine Surgery Zhengzhou Orthopedics HospitalZhengzhouHenan ProvinceChina department of Orthopedic Surgery and Orthopedic Research Institute West China HospitalSichuan UniversityChengduSichuan ProvinceChina department of Orthopedics Xinqiao HospitalArmy Medical UniversityChongqingChina department of Orthopedics The First Affiliated Hospital of China Medical UniversityShenyangLiaoning ProvinceChina department of Spinal Surgery Shengj

Traumatic spinal cord injury is potentially catastrophic and can lead to permanent disability or even *** has the largest population of patients with traumatic spinal cord *** studies of traumatic spinal cord injury in China have mostly been regional in scope;national-level studies have been *** the best of our knowledge,no national-level study of treatment status and economic burden has been *** retrospective study aimed to examine the epidemiological and clinical features,treatment status,and economic burden of traumatic spinal cord injury in China at the national *** included 13,465 traumatic spinal cord injury patients who were injured between January 2013 and December 2018 and treated in 30 hospitals in 11 provinces/municipalities representing all geographical divisions of *** epidemiological and clinical features,treatment status,and total and daily costs were *** in the percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department and cost of care were assessed by annual percentage change using the Joinpoint Regression *** percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department did not significantly change overall(annual percentage change,-0.5%and 2.1%,respectively).A total of 10,053(74.7%)patients underwent *** 2.8%of patients who underwent surgery did so within 24 hours of injury.A total of 2005(14.9%)patients were treated with high-dose(≥500 mg)methylprednisolone sodium succinate/methylprednisolone(MPSS/MP);615(4.6%)received it within 8 *** total cost for acute traumatic spinal cord injury decreased over the study period(-4.7%),while daily cost did not significantly change(1.0%increase).Our findings indicate that public health initiatives should aim at improving hospitals’ability to complete early surgery within 24 ho

关键词： China clinical features costs epidemiology methylprednisolone sodium succinate methylprednisolone retrospective study traumatic spinal cord injury treatment

Targeting BPOZ-2 in Lewy body disease

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2016年第6期11卷 910-911页

作者： Avik Roy department of neurological sciences Rush University Medical Center

Formation of Lewy body inclusions（LBs）in the substantia nigra（SN）is a very well-characterized pathological hallmark of Parkinson’s disease（PD）.LBs are aggregates of many biologically inactive proteins including structural elements,alpha-synuclein（asyn）-binding proteins,synphilin-1-binding proteins,and components of the ubiquitin-proteasome system.

关键词： Targeting substantia proteasome nigra ubiquitin biologically inactive mitochondria dopamine parkin

Multimodality management for chronic subdural hematoma in China: protocol and characteristics of an ambidirectional, nationwide, multicenter registry study

在线全文

学校读者我要写书评

暂无评论

中华神经外科杂志(英文) 2024年第2期10卷 1-10页

作者： Liu Tao Zhao Zhihao Huang Jinhao Zhu Xide Chen Weiliang Lin Kun Yu Yunhu Li Zhanying Fan Yibing Liu Mingqi Nie Meng Liu Xuanhui Gao Chuang Quan Wei Qian Yu Wu Chenrui Yuan Jiangyuan Wu Di Lv Chuanxiang Dong Shiying Mi Liang Tian Yu Tian Ye Zhang Jianning Jiang Rongcai department of Neurosurgery Tianjin Medical University General Hospital Tianjin China Ministry of Education Tianjin Neurological Institute Key Laboratory of Post Neuro-injury Neuro-repair and Regeneration in Central Nervous System Tianjin Medical University General Hospital Tianjin China department of Neurosurgery Linyi People’s Hospital Shandong China department of Neurosurgery Haining People’s Hospital Zhejiang China department of Neurosurgery Fujian Provincial Hospital Fujian China department of Clinical Research Center for neurological Disease the People’s Hospital of HongHuaGang District of ZunYi Guizhou China department of Neurosurgery Kailuan General Hospital Hebei China department of Neurosurgery Tianjin First Central Hospital Tianjin China department of Neurosurgery The First Hospital of Jilin University Changchun China

Background Despite its prevalence, there is ongoing debate regarding the optimal management strategy for chronic subdural hematoma (CSDH), reflecting the variability in clinical presentation and treatment outcomes. This ambidirectional, nationwide, multicenter registry study aims to assess the efficacy and safety of multimodality treatment approaches for CSDH in the Chinese ***/design A multicenter cohort of CSDH patients from 59 participating hospitals in mainland China was enrolled in this study. The treatment modalities encompassed a range of options and baseline demographics, clinical characteristics, radiographic findings, and surgical techniques were documented. Clinical outcomes, including hematoma resolution, recurrence rates, neurological status, and complications, were assessed at regular intervals during treatment, 3 months, 6 months, 1 year, and 2 years *** Between March 2022 and August 2023, a comprehensive cohort comprising 2173 individuals who met the criterion was assembled across 59 participating clinical sites. Of those patients, 81.1% were male, exhibiting an average age of 70.12 ± 14.53 years. A historical record of trauma was documented in 48.0% of cases, while headache constituted the predominant clinical presentation in 58.1% of patients. The foremost surgical modality employed was the burr hole (61.3%), with conservative management accounting for 25.6% of cases. Notably, a favorable clinical prognosis was observed in 88.9% of CSDH patients at 3 months, and the recurrence rate was found to be 2.4%.Conclusion This registry study provides critical insights into the multimodality treatment of CSDH in China, offering a foundation for advancing clinical practices, optimizing patient management, and ultimately, improving the quality of life for individuals suffering from this challenging neurosurgical *** registration ChiCTR2200057179

关键词： Chronic subdural hematoma Multimodality therapy Outcome Recurrence

New insights into the therapeutic approaches for the treatment of tauopathies

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第5期19卷 1020-1026页

作者： Himanshi Singh Asmita Das Mohammad Moshahid Khan Tayebeh Pourmotabbed department of Microbiology Immunology and BiochemistryUniversity of Tennessee Health Science CenterMemphisTNUSA department of Biotechnology Delhi Technological UniversityShahbad DaulatpurDelhiIndia department of Neurology College of MedicineUniversity of Tennessee Health Science CenterMemphisTNUSA Neuroscience Institute University of Tennessee Health Science CenterMemphisTNUSA Center for Muscle Metabolism and NeuropathologyDivision of Regenerative and Rehabilitation Sciences and Department of Physical TherapyCollege of Health ProfessionsUniversity of Tennessee Health Science CenterMemphisTNUSA

Tauopathies are a group of neurological disorders,including Alzheimer’s disease and frontotemporal dementia,which involve progressive neurodegeneration,cognitive deficits,and aberrant tau protein *** development of tauopathies cannot currently be stopped or slowed down by treatment *** the significant contribution of tau burden in primary tauopathies and the strong association between pathogenic tau accumulation and cognitive deficits,there has been a lot of interest in creating therapies that can alleviate tau pathology and render neuroprotective ***,small molecules,immunotherapies,and gene therapy have been used to reduce the pathological tau burden and prevent neurodegeneration in animal models of ***,the major pitfall of the current therapeutic approach is the difficulty of drugs and gene-targeting modalities to cross the blood-brain barrier and their unintended side *** this review,the current therapeutic strategies used for tauopathies including the use of oligonucleotide-based gene therapy approaches that have shown a promising result for the treatment of tauopathies and Alzheimer’s disease in preclinical animal models,have been discussed.

关键词： dementia gene therapies immunotherapy neurodegeneration oligonucleotides tau tauopathies therapies

Small extracellular vesicles derived from cerebral endothelial cells with elevated microRNA 27a promote ischemic stroke recovery

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 224-233页

作者： Yi Zhang Zhongwu Liu Michael Chopp Michael Millman Yanfeng Li Pasquale Cepparulo Amy Kemper Chao Li Li Zhang Zheng Gang Zhang department of Neurology Henry Ford HospitalDetroitMIUSA department of Physics Oakland UniversityRochesterMIUSA department of Pathology Henry Ford HospitalDetroitMIUSA Division of Pharmacology Department of NeuroscienceReproductive and Dentistry SciencesSchool of MedicineUniversity of Naples“Federico Ⅱ”NaplesItaly

Axonal remodeling is a critical aspect of ischemic brain repair processes and contributes to spontaneous functional *** previous in vitro study demonstrated that exosomes/small extracellular vesicles(sEVs)isolated from cerebral endothelial cells(CEC-sEVs)of ischemic brain promote axonal growth of embryonic cortical neurons and that microRNA 27a(miR-27a)is an elevated miRNA in ischemic *** the present study,we investigated whether normal CEC-sEVs engineered to enrich their levels of miR-27a(27a-sEVs)further enhance axonal growth and improve neurological outcomes after ischemic stroke when compared with treatment with non-engineered CEC-sEVs.27a-sEVs were isolated from the conditioned medium of healthy mouse CECs transfected with a lentiviral miR-27a expression *** EVs isolated from CECs transfected with a scramble vector(Scra-sEVs)were used as a *** male mice were subjected to permanent middle cerebral artery occlusion and then were randomly treated with 27a-sEVs or *** array of behavior assays was used to measure neurological *** with treatment of ischemic stroke with Scra-sEVs,treatment with 27a-sEVs significantly augmented axons and spines in the peri-infarct zone and in the corticospinal tract of the spinal grey matter of the denervated side,and significantly improved neurological *** vitro studies demonstrated that CEC-sEVs carrying reduced miR-27a abolished 27a-sEV-augmented axonal *** analysis revealed that 27a-sEVs systemically administered preferentially localized to the pre-synaptic active zone,while quantitative reverse transcription-polymerase chain reaction and Western Blot analysis showed elevated miR-27a,and reduced axonal inhibitory proteins Semaphorin 6A and Ras Homolog Family Member A in the peri-infarct *** of the Clathrin-dependent endocytosis pathway substantially reduced neuronal internalization of *** data provide evidence that 27a-sEVs have

关键词： axonal remodeling cerebral endothelial cells exosomes miR-27a mitochondria Semaphorin 6A small extracellular vesicles stroke