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Transforming growth factor-beta 1 enhances discharge activity of cortical neurons

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Neural Regeneration Research 2025年第2期20卷 548-556页

作者： Zhihui Ren Tian Li Xueer Liu Zelin Zhang Xiaoxuan Chen Weiqiang Chen Kangsheng Li Jiangtao Sheng department of microbiology and Immunology Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular ImmunopathologyShantou University Medical CollegeShantouGuangdong ProvinceChina department of Neurosurgery First Affiliated Hospital of Shantou University Medical CollegeShantouGuangdong ProvinceChina

Transforming growth factor-beta 1(TGF-β1)has been extensively studied for its pleiotropic effects on central nervous system *** neuroprotective or neurotoxic effects of TGF-β1 in specific brain areas may depend on the pathological process and cell types ***-gated sodium channels(VGSCs)are essential ion channels for the generation of action potentials in neurons,and are involved in various neuroexcitation-related ***,the effects of TGF-β1 on the functional properties of VGSCs and firing properties in cortical neurons remain *** this study,we investigated the effects of TGF-β1 on VGSC function and firing properties in primary cortical neurons from *** found that TGF-β1 increased VGSC current density in a dose-and time-dependent manner,which was attributable to the upregulation of Nav1.3 *** VGSC current density and Nav1.3 expression were significantly abolished by preincubation with inhibitors of mitogen-activated protein kinase kinase(PD98059),p38 mitogen-activated protein kinase(SB203580),and Jun NH2-terminal kinase 1/2 inhibitor(SP600125).Interestingly,TGF-β1 significantly increased the firing threshold of action potentials but did not change their firing rate in cortical *** findings suggest that TGF-β1 can increase Nav1.3 expression through activation of the ERK1/2-JNK-MAPK pathway,which leads to a decrease in the firing threshold of action potentials in cortical neurons under pathological ***,this contributes to the occurrence and progression of neuroexcitatory-related diseases of the central nervous system.

关键词： central nervous system cortical neurons ERK firing properties JNK Nav1.3 p38 transforming growth factor-beta 1 traumatic brain injury voltage-gated sodium currents

Convergence of COVID-19 and recurrent stroke:In-hospital mortality risks explored

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World Journal of Virology 2025年第1期14卷 5-8页

作者： Basavraj S Nagoba Shree V Dhotre Ajay M Gavkare Sachin S Mumbre Pradnya S Dhotre department of microbiology Maharashtra Institute of Medical Sciences and Research(Medical College)Latur 413531India department of microbiology Ashwini Rural Medical CollegeSolapur 413001India department of Community Medicine Ashwini Rural Medical CollegeSolapur 413001India department of Biochemistry Ashwini Rural Medical CollegeSolapur 413001India

This editorial comments on the article by Desai et al,which investigates the impact of coronavirus disease 2019(COVID-19)on in-hospital mortality among patients with recurrent stroke using data from the 2020 National Inpatient *** findings reveal significantly higher mortality rates in COVID-19-positive patients compared to non-COVID-19 patients,particularly among middle-aged individuals,males,and ethnic *** editorial explores the underlying mechanisms contributing to these outcomes and discusses the clinical implications for targeted management strategies in high-risk *** results emphasize the need for comprehensive approaches to mitigate the heightened risks faced by recurrent stroke patients during the COVID-19 pandemic.

关键词： Recurrent stroke COVID-19 In-hospital mortality Nationwide analysis Stroke admissions Infectious diseases Chronic health conditions Hypercoagulability

Gut virome:New key players in the pathogenesis of inflammatory bowel disease

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World Journal of Methodology 2025年第2期15卷 27-43页

作者： Helal F Hetta Rehab Ahmed Yasmin N Ramadan Hayam Fathy Mohammed Khorshid Mohamed M Mabrouk Mai Hashem department of Medical microbiology and Immunology Faculty of MedicineAssiut UniversityAssiut 71515Egypt Division of microbiology Immunology and BiotechnologyFaculty of pharmacyUniversity of TabukTabuk 71491Saudi Arabia Division of microbiology Immunology and BiotechnologyDepartment of Natural Products and Alternative MedicineFaculty of PharmacyUniversity of TabukTabuk 71491Saudi Arabia department of Internal Medicine Division HepatogastroenterologyAssiut UniversityAssiut 71515Egypt department of Clinical Research Egyptian Developers of Gastroenterology and Endoscopy FoundationCairo 11936Egypt department of Internal Medicine Faculty of Medicine.Tanta UniversityTanta 31527Egypt department of Tropical Medicine Gastroenterology and HepatologyAssiut University HospitalAssiut 71515Egypt

Inflammatory bowel disease(IBD)is a chronic inflammatory illness of the *** the mechanism underlying the pathogenesis of IBD is not fully under-stood,it is believed that a complex combination of host immunological response,environmental exposure,particularly the gut microbiota,and genetic suscept-ibility represents the major *** gut virome is a group of viruses found in great frequency in the gastrointestinal tract of *** gut virome varies greatly among individuals and is influenced by factors including lifestyle,diet,health and disease conditions,geography,and *** majority of research has focused on the significance of gut bacteria in the progression of IBD,although viral populations represent an important component of the *** conducted this review to highlight the viral communities in the gut and their expected roles in the etiopathogenesis of IBD regarding published research to date.

关键词： Inflammatory bowel disease Pathogenesis Gut virome Bacteriophage Eukaryotic viruses

Unveiling the impact:COVID-19's influence on bacterial resistance in the Kingdom of Bahrain

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World Journal of Virology 2025年第1期14卷 83-98页

作者： Nermin K Saeed Safiya K Almusawi Noor A Albalooshi Mohammed Al-Beltagi Medical microbiology Section Department of PathologySalmaniya Medical ComplexGovernmental HospitalsManama 12Bahrain Medical microbiology Section Department of PathologyRoyal College of Surgeons in Ireland-Medical University of BahrainBusaiteen 15503MuharraqBahrain department of Paediatrics Faculty of MedicineTanta UniversityTanta 31511AlghrabiaEgypt department of Pediatric University Medical CenterKing Abdulla Medical CityArabian Gulf UniversityManama 26671AlgharbiaBahrain

BACKGROUND Antibiotic resistance is a growing global health threat,and understanding local trends in bacterial isolates and their susceptibility patterns is crucial for effective infection control and antimicrobial *** coronavirus disease 2019(COVID-19)pandemic has introduced additional complexities,potentially influencing these *** To analyze trends in bacterial isolates and their antibiotic susceptibility patterns at Salmaniya Medical Complex from 2018 to 2023,with a specific focus on the impact of the COVID-19 pandemic on these *** A retrospective analysis of microbiological data was conducted,covering the period from 2018 to *** study included key bacterial pathogens such as Escherichia coli(***),Klebsiella pneumoniae,Acinetobacter baumannii,Pseudomonas aeruginosa,and Staphylococcus aureus,among *** antibiotic susceptibility profiles of these isolates were assessed using standard laboratory *** contextualize the findings,the findings were compared with similar studies from other regions,including China,India,Romania,Saudi Arabia,the United Arab Emirates,Malaysia,and United *** The study revealed fluctuating trends in the prevalence of bacterial isolates,with notable changes during the COVID-19 *** example,a significant increase in the prevalence of Staphylococcus aureus was observed during the pandemic years,while the prevalence of *** showed a more variable *** resistance rates varied among the different pathogens,with a concerning rise in resistance to commonly used antibiotics,particularly among Klebsiella pneumoniae and ***,the study identified an alarming increase in the prevalence of multidrug-resistant(MDR)strains,especially within Klebsiella pneumoniae and *** *** impact of the COVID-19 pandemic on these trends was evident,with shifts in the frequency,resistance patterns,and the emergence of MDR bacteria among several key ***

关键词： Multidrug-resistant organisms Antibiotic susceptibility COVID-19 pandemic Antimicrobial stewardship Bacterial isolates Salmaniya Medical Complex Bahrain

Synthesis, characterization and antimicrobial activity of zinc and cerium co-doped α-zirconium phosphate

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Journal of Rare Earths 2012年第8期30卷 820-825页

作者：代光剑于爱丽蔡祥施庆珊欧阳友生谭绍早 State Key Laboratory of applied microbiology(Ministry-Guangdong Province Jointly Breeding Base) South ChinaGuangdong Institute of Microbiology department of Chemistry Jinan University

A series of zinc ions or/and cerium ions co-doped a-zirconium phosphate （Zn-Ce@ZrPs） were prepared. The novel Zn-Ce@ZrPs were characterized and the antibacterial activity on Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus were tested. The results showed that zinc ions （Zn2＋） or/and cerium ions （Ce3＋） were combined with ZrP, and the Ce3＋ was adsorbed on the surface of ZrP through hydrogen bonds, while Zn2＋ intercalated into the interlayer of ZrP. Zn-Ce@ZrPs showed excellent synergistic antibacterial activity. When Zn2＋/Ce3＋ atomic ratio was 0.6, the Zn-Ce@ZrP3 showed the highest synergistic antibacterial efficiency, suggesting great potential ap- plication as antibacterial agents in microbial control.

关键词： zinc ion cerium ion α-zirconium phosphate structure synergetic antibacterial rare earths

Immunohistochemical expression of matrix metalloproteinase-9 and 13 in oral squamous cell carcinoma and their role in predicting lymph node metastasis

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World Journal of Methodology 2025年第2期15卷 108-116页

作者： Bhari Sharanesha Manjunatha Keshav T Handge Vandana Sandeep Shah Yasser Eid Al-Thobaiti Deepak Gowda Sadashivappa Pateel department of Basic Oral Medicine and Allied Dental Sciences Taif UniversityAt`Taif 26571MakkahSaudi Arabia department of Oral and Maxillofacial Pathology Dr.Vasantrao Pawar Medical CollegeHospital and Research CentreNashik 423101MaharashtraIndia department of Oral Pathology and microbiology KM Shah Dental College and HospitalSumandeep VidyapeethVadodara 391760GujaratIndia department of Oral and Maxillofacial Surgery and Diagnostic Sciences Faculty of DentistryTaif UniversityAl-Haweiah 26571 MakkahSaudi Arabia department of Oral Pathology and microbiology Faculty of DentistryMAHSA UniversitySelangor 42610Malaysia

BACKGROUND One of the main characteristics of oral squamous cell carcinoma(OSCC)is that it metastasizes to cervical lymph nodes frequently with a high degree of local invasiveness.A primary feature of malignant tumors is their penetration of neighboring tissues,such as lymphatic and blood arteries,due to the tumor cells'capacity to break down the extracellular matrix(ECM).Matrix metalloproteinases(MMPs)constitute a family of proteolytic enzymes that facilitate tissue remo-deling and the degradation of the ***-9 and MMP-13 belong to the group of extracellular matrix degrading enzymes and their expression has been studied in OSCC because of their specific ***-13,a collagenase family member,is thought to play an essential role in the MMP activation cascade by breaking down the fibrillar collagens,whereas MMP-9 is thought to accelerate the growth of *** MMP-13 expression has been associated with tumor behavior and patient prognosis in a number of malignant *** authors wish to thank Jadhav KB for his valuable opinion during the preparation of the manuscript.

关键词： Matrix metalloproteinases Oral squamous cell carcinoma Tumor staging Immunohistochemistry Invasion Lymph node metastasis TNM stage

Bioinhibition of diarrhogenic Gram-positive bacterial pathogens by potential indigenous probiotics

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Asian Pacific Journal of Tropical Medicine 2008年第2期1卷 7-11页

作者： Adenike A.O.Ogunshe applied microbiology and Infectious Diseases Department of Botany & MicrobiologyUniversity of IbadanIbadanNigeria

High level infant mortality rates and onset of drug resistance has led into the possible development of indigenous probiotics as alternative bacteriotherapy in the control of infantile bacterial *** study was to determine the in vitro inhibitory potential of four probiotic candidates obtained from Nigerian indigenous fermented foods and beverages and from faecal specimens of healthy infants on infantile Gram-positive diarrhogenic bacterial *** probiotic candidates,AAOOL4,*** AAOOCH1,*** AA0025NN and *** AAOOT20 were assayed for in vitro bactericidal effects on diarrhogenic bacterial test strains-Bacillus cereus 25S,*** 32S,*** 26S and *** *** the test strains inoculated into an industrial infant weaning food already seeded with the probiotic strains were significantly inhibited within 96 *** AAOOL4,*** AAOOCH1,*** AAOO25NN and *** AAOOT20 had in vitro bactericidal effects on bacteri isolates implicated in infantile diarrhoea,indicating the probiotic potential of the candidates.

关键词： bactericidal biotherapy diarrhoea infantile probiotics tropics

Dissociation of outer membrane for Escherichia coli cell caused by cerium nitrate

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Journal of Rare Earths 2010年第2期28卷 312-315页

作者：陈爱美施庆珊冯劲欧阳友生陈仪本谭绍早 Guangdong Institute of microbiology Guangdong Open Laboratory of applied microbiology Guangdong Province Key Laboratory of Microbial Culture Collection and Application department of Chemistry Jinan University

The biological effect of cerium nitrate on the outer membrane(OM) of Escherichia coli(***) cell was studied,and the antim-icrobial mechanism of rare earth elements was *** antimicrobial effect of cerium nitrate on *** cell was valued by plate count method,and the morphology change of *** cell was observed with scanning electron microscopy(SEM) and transmission electron microscopy(TEM).The results showed that the *** cell suspension was flocculated when the concentration of Ce(NO3)3?6H2O...

关键词： cerium nitrate Escherichia coli outer membrane scanning electron microscope transmission electron microscope morphology rare earths

Establishment of a simple assay in vitro for hepatitis C virus NS3 serine protease based on recombinant substrate and single-chain protease

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World Journal of Gastroenterology 2002年第6期8卷 1088-1093页

作者： Gui-Xin Du Li-Hua Hou Rong-Bin Guan Yi-Gang Tong Hai-Tao Wang,department of applied Molecular Biology,Institute of microbiology and Epidemiology,Fengtai,Beijing 100071,China department of applied Molecular Biology Institute of Microbiology and Epidemiology Fengtai Beijing 100071 China. dugx@***

AIM: To establish a simple and convenient assay in vitro for the Hepatitis C virus NS3 serine protease based on the recombinant protease and substrate, and to evaluate its feasibility in screening the enzyme inhibitors. METHODS: Based on the crystallographic structure of hepatitis C virus (HCV) serine protease, a novel single-chain serine protease was designed, in which the central sequence of cofactor NS4A was linked to the N-terminus of NS3 serine protease domain via a flexible linker GSGS. The fusion gene was obtained by two-step PCR that was carried out with three primers and then cloned into the prokaryotic expression vector pQE30, and the recombinant clone was verified by DNA sequencing. The single-chain recombinant protease was expressed when the *** induced with IPTG and the expression conditions were optimized to produce large amount of soluble protease. The recombinant substrate NS5ab that covers the cleavage point NS5A/B was also expressed in ***. Both of the protease and substrate were purified by using Ni-NTA agarose metal affinity resin, then they were mixed together in a specific buffer, and the mixture was analyzed by SDS-PAGE. The cleavage system was used to evaluate some compounds for their inhibitory activity on serine ***: The single-chain recombinant protease was overexpressed as soluble protein when the E. coliwas induced at a low dosage of IPTG (0.2 mM) and cultured at a low temperature (15℃). The protease was purified by using Ni-NTA agarose metal affinity resin (the purity is over 95 %).The recombinant substrate NS5ab was expressed in an insoluble form and could refold successfully after purification and dialysis. A simple and convenient assay in vitro was established, in which the purified single-chain serine protease could cleave the recombinant substrate NS5ab into two fragments that were visualized by SDS-PAGE. PMSF had an effect on inhibiting activity of serine protease, while EDTA had ***: A simple and

关键词： C肝病毒 NS3丝氨酸蛋白酶体外简易检验法重组基质单链蛋白酶