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Insect Science 2011年第5期18卷 583-589页

作者： M. J. Lopez-Rodriguez J. M. Tierno de Figueroa J. Alba-Tercedor departamento de biologia Animal Facultad de Ciencias Universidad de Granada Granada Spain

The nymphal biology of a population of Torleya major （Klapalek） in southem Iberian Peninsula was studied. An atypical life cycle pattern is described, with eggs hatching in August producing a fast-developing cohort with adults emerging in autumn and a second slow-developing cohort with adults emerging in spring of the following year. Nymphal growth occurred primarily in summer-autumn （in the first cohort） and in spring （in the second）. The origin of such a life history is discussed. Nymphs were collector-gatherers, consuming mainly detritus. Although ontogenetic shifts on the use of trophic resources were detected, similar food was utilized during the months when both cohorts cohabited, eliminating the possibility that the rapid growth of the first cohort could be related to the utilization of different food resources.

关键词： collector-gatherer life cycle mayfly nymphal feeding southern Iberian Peninsula

Diagnosis of Laron syndrome using monoplex-polymerase chain reaction technology with a whole-genome amplification template: A case report

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World Journal of Clinical Cases 2019年第23期7卷 4029-4035页

作者： Adina Neumann Miguel Angel Alcantara-Ortigoza Ariadna Gonzalez-del Angel Felipe Camargo-Diaz Esther Lopez-Bayghen Laboratorio de Investigacion y Diagnostico molecular Instituto de Infertilidad y Genetica Mexico SCINGENESMexico City 05320Mexico Instituto Nacional de Pediatria Torre de InvestigacionMexico City 04530Mexico Laboratorio de biologia molecular Departamento de Genetica HumanaInstituto Nacional de PediatriaMexico City 04530Mexico departamento de Toxicologia Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional(CINVESTAV-IPN)Mexico City 07360Mexico

BACKGROUND Laron syndrome(LS)is an autosomal recessive hereditary condition affecting only 1/1000000 *** cause is associated with mutations in the growth hormone(GH)receptor(GHR),leading to GH *** patients typically present with severe growth retardation,obesity,and abnormal sexual ***,LS diagnosis is performed ***,we assessed the efficiency of Pre-implantation Genetic Testing(PGT)coupled with monoplex-polymerase chain reaction(PCR)technology for detecting this monogenic disease in embryos from a couple confirmed as LS heterozygous carriers CASE SUMMARy The couple LS-carriers were confirmed by the presence of a first child born with *** couple underwent a standard in vitro fertilization(IVF)*** was collected from trophectoderm cells from day 5 *** genome amplification(WGA)was performed using a Sureplex DNA Amplification System and analyzed by PCR,targeting the deletion of the exons 5 and 6 in the GHR gene as well as PGT by Next-generation Sequencing(Illumina).Eleven embryos were collected and analyzed.27.3%were the wild type for GHR,45.5%were heterozygotes,and 18.2%homozygous *** embryo yielded no *** 2-embryos transfers were performed;2 normal homozygous and four heterozygous carriers were selected for *** first two transfers were unsuccessful,whereas the final transfer with two heterozygous embryos resulted in clinical *** genomic composition of the fetus was verified,applying the same techniques using amniocytes,extracted after 21 wk of the ongoing *** fetus was confirmed as GHR deletion in exon 5-6,carrier.A non-affected baby was *** Here,we present a case demonstrating that using WGA as a template in addition to PCR targeting specific gene regions,exons 5 and 6 on the GHR gene,could identify LS carrier *** provides evidence that WGA and PCR serve as an excellent tool to detect this specific monogenic disease in IVF e

关键词： Growth hormone insensitivity Growth hormone receptor mutations Intragenic deletions molecular diagnosis Embryo diagnosis Laron syndrome Case report

Using dung beetles to evaluate the effects of urbanization on Atlantic Forest biodiversity

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Insect Science 2013年第3期20卷 393-406页

作者： Vanesca Korasaki Jose Lopes George Gardner Brown Julio Louzada departamento de Agronomia departamento de biologia Animal e Vegetal Universidade Estadual de Londrina Londrina-PR Empresa Brasileira de Pesquisa Agropecu~ria Embrapa Florestas Colombo-PR departamento de biologia Setor de Ecologia Universidade Federal de Lavras Lavras Minas Gerais Brazil

We used dung beetles to evaluate the impact of urbanization on insect biodi- versity in three Atlantic Forest fragments in Londrina, Parana, Brazil. This study provides the first empirical evidence of the impact of urbanization on richness, abundance, compo- sition and guild structure of dung beetle communities from the Brazilian Atlantic Forest. We evaluated the community aspects （abundance, richness, composition and food guilds） of dung beetles in fragments with different degrees of immersion in the urban matrix using pitfall traps with four alternative baits （rotten meat, rotten fish, pig dung and decaying ba- nana）. A total of 1 719 individuals were collected, belonging to 29 species from 11 genera and six Scarabaeinae tribes. The most urban-immersed fragment showed a higher species dominance and the beetle community captured on dung presented the greatest evenness. The beetle communities were distinct with respect to the fragments and feeding habits. Except for the dung beetle assemblage in the most urbanized forest fragment, all others exhibited contrasting differences in species composition attracted to each bait type. Our results clearly show that the degree of urbanization affects Atlantic Forest dung beetle communities and that the preservation of forest fragments inside the cities, even small ones, can provide refuges for Scarabaeinae.

关键词： bioindicator coprophagous beetles food preference fragmentation habitat loss urban matrix

Vitellogenin content in fat body and ovary homogenates of workers and queens of Melipona quadrifasciata anthidioides during vitellogenesis

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Insect Science 2012年第2期19卷 213-219页

作者： Vagner Tadeu Paes de Oliveira Bruno Berger Carminda da Cruz-Landim Zila Luz Paulino Simoes departamento de biologia Instituto de Biociencias de Rio ClaroUNESP-Univ.Bela VistaRio ClaroSao Paulo departamento de biologia FFFL de Ribeirao PretoUniversidade de Sao PauloUSPSao PauloBrazil

Vitellogenin （Vg） is an egg yolk protein that is produced primarily in the fat body of most female insects. In the advanced social structure of eusocial honeybees, the presence of the queen inhibits egg maturation in the workers＇ ovaries. However in the stingless bee Melipona quadrifasciata, the workers always develop ovaries and lay a certain amount of eggs while provisioning the brood cells with larval food during what is known as the worker nurse phase. The present work is a comparative study of the presence of Vg in homogenates of the fat bodies and ovaries of the nurse workers, and the virgin and physogastric queens of M. quadrifasciata. The presence of Vg was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and irnmunoblotting using Apis mellifera anti-egg antibody. Vg was not detected in the fat bodies or ovaries of the workers, but it was found in the ovaries of virgin and physogastric queens and in the fat body ofphysogastric queens. The results are discussed, taking into account the reproductive state of the individuals and the other possible roles of Vg, such as a storage protein for metoabolism of other organs.

关键词： egg yolk electrophoresis immunoblotting nurse worker stingless bee virginand physogastric queen

Chlamydia trachomatis and sperm lipid peroxidation in infertile men

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Asian Journal of Andrology 2003年第1期5卷 47-49页

作者： A.Segnini M.I.Camejo F.Proverbio Centro de Biofisica y Bioquimica Instituto Venezolano de Investigaciones Cientlficas VenezuelaDepartamento de Biologia de Organismos Universidad Simon Bolivar Miranda VenezuelaCentro de Biofisica y Bioquimica Instituto Venezolano de Investigaciones Cientificas Venezuela.

Aim: To relate the presence of anti-Chlamydial trachomatis IgA in semen with sperm lipid membrane peroxidation and changes in seminal parameters. Methods: Semen samples of the male partners of 52 couples assessed for undiagnosed infertility were examined for the presence of IgA antibody against C. trachomatis. The level of sperm membrane lipid peroxidation was estimated by determining the malondialdehyde (MDA) formation. Results: Sperm membrane of infertile males with positive IgA antibodies against C. trachomatis showed a higher level of lipid peroxidation than that of infertile males with negative IgA antibody (Py and the magnitude of sperm membrane lipid peroxidation. All the other tested semen parameters were found to be similar in the two groups. Conclusion: The activation of immune system by C. trachomatis may promote lipid peroxidation of the sperm membrane. This could be the way by which C. trachomatis affects fertility.

关键词： Chlamydia trachomatis lipid peroxidation spermatozoa male infertility

The FAIR Data Point:Interfaces and Tooling

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Data Intelligence 2023年第1期5卷 184-201页

作者： Oussama Mohammed Benhamed Kees Burger Rajaram Kaliyaperumal Luiz Olavo Bonino da Silva Santos Marek Suchánek Jan Slifka Mark D.Wilkinsoni departamento de Biotecnologia-biologia Vegetal Escuela Tecnica Superior de Ingenieria AgronomicaAlimentaria y de BiosistemasCentro de Biotecnologia y Genomica de Plantas UPM-INIAUniversidad Politecnica de Madrid(UPM)-Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria(INIA)MadridES 28223Spain Leiden University Medical Center Leiden2333 ZCthe Netherlands University of Twente Enschede7513 GBthe Netherlands Czech Technical University in Prague PragueCZ 15000Czech Republic

While the FAIR Principles do not specify a technical solution for'FAIRness',it was clear from the outset of the FAIR initiative that it would be useful to have commodity software and tooling that would simplify the creation of FAIR-compliant *** FAIR Data Point is a metadata repository that follows the DCAT(2)schema,and utilizes the Linked Data Platform to manage the hierarchical metadata layers as LDP *** has been a recent flurry of development activity around the FAIR Data Point that has significantly improved its power and *** we describe five specific tools—an installer,a loader,two Webbased interfaces,and an indexer-aimed at maximizing the uptake and utility of the FAIR Data Point.

关键词： FAIR Data Linked data Semantic Web Metadata User interfaces Tooling Semantic query

Down-regulation of Fra a 1.02 in strawberry fruits causes transcriptomic and metabolic changes compatible with an altered defense response

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Horticulture Research 2021年第1期8卷 777-789页

作者： Begona Orozco-Navarrete Jina Song Ana Casanal Rosangela Sozzani Victor Flors Jose F.Sanchez-Sevilla Johanna Trinkl Thomas Hoffmann Catharina Merchante Wilfried Schwab Victoriano Valpuesta Laboratorio de Bioquimica y Biotecnologia Vegetal Instituto de Hortofruticultura Subtropical y Mediterranea(IHSM)Universidad de Malaga-Consejo Superior de Investigaciones CientificasDepartamento de Biologia Molecular y BioquimicaFacultad de CienciasUMAMalagaSpain department of Plant and Microbial Biology North Carolina State UniversityRaleighNCUSA Metabolic Integration and Cell Signalling Group Plant Physiology SectionDepartment of Ciencias Agrarias y del Medio NaturalUniversitat Jaume ICastelloSpain departamento de genomica y Biotecnologia IFAPAMalagaSpain Biotechnology of Natural Products Technische Universität MünchenLiesel-Beckmann-Str.185354 FreisingGermany

The strawberry Fra a 1 proteins belong to the class 10 Pathogenesis-Related(PR-10)*** strawberry,a large number of members have been identified,but only a limited number is expressed in the *** this organ,Fra a 1.01 and Fra a 1.02 are the most abundant Fra proteins in the green and red fruits,respectively,however,their function remains *** know the function of Fra a 1.02 we have generated transgenic lines that silence this gene,and performed metabolomics,RNA-Seq,and hormonal *** studies associated Fra a 1.02 to strawberry fruit color,but the analysis of anthocyanins in the ripe fruits showed no diminution in their content in the silenced *** ontology(GO)analysis of the genes differentially expressed indicated that oxidation/reduction was the most represented biological *** state was not apparently altered since no changes were found in ascorbic acid and glutathione(GSH)reduced/oxidized ratio,but GSH content was reduced in the silenced *** addition,a number of glutathione-S-transferases(GST)were down-regulated as result of Fra a *** highly represented GO category was transport which included a number of ABC and MATE *** the regulatory genes differentially expressed WRKy33.1 and WRKy33.2 were down-regulated,which had previously been assigned a role in strawberry plant defense.A reduced expression of the VQ23 gene and a diminished content of the hormones JA,SA,and IAA were also *** data might indicate that Fra a 1.02 participates in the defense against pathogens in the ripe strawberry fruits.

关键词： straw content fruits

Gustatory responsiveness in Vespula germanica workers:exploring the interplay between sensory perception and task specialization

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Insect Science 2024年第2期31卷 587-598页

作者： Analia Mattiacci Carolina Mengoni Gonalons MaitéMasciocchi Juan C.Corley Grupo de Ecologia de Poblaciones de Insectos IFAB(CONICETINTA EEA Bariloche)BarilocheArgentina departamento de Ecologia Centro Regional Universitario BarilocheUniversidad Nacional Del ComahueBarilocheArgentina departamento de Biodiversidad y biologia Experimental Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresBuenos AiresArgentina

Workers'task specialization and division of labor are critical features of social insects'ecological *** has been proposed that the division of labor relies on response threshold models:individuals varying their sensitivity(and responsiveness)to biologically relevant stimuli and performing a specific task when a stimulus exceeds an internal *** this work,we study carbohydrate and protein responsiveness and their relation to worker task specialization in Vespula germanica,an invasive social *** sucrose and peptone responsiveness of two different subcastes,preforagers and foragers,was determined by stimulating the antenna of the wasps with increasing concentrations of the solution and quantifying whether each concentration elicited a licking *** studied responsiveness in five different ways:(1)response threshold,(2)concentration 50(concentration to which at least 50%of wasps responded),(3)maximum response,(4)mean scores and(5)median *** results suggest that V germanica foragers are more sensitive to sucrose(lower thresholds)than preforager ***,we found no differences for peptone thresholds(i.e.,a protein resource).Nonetheless,this is the first study to investigate response thresholds for protein *** intercaste variation in sucrose responsiveness shown in our work contributes to the existing knowledge about response threshold theory as a mechanism for task specialization observed in V germanica.

关键词： carbohydrates responsiveness division of labor protein responsiveness response thresholds models social insect yellow jacket

Acromyrmex ameliae sp. n. （Hymenoptera： Formicidae）： A new social parasite of leaf-cutting ants in Brazil

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Insect Science 2007年第3期14卷 251-257页

作者： DANIVAL JOSE de SOUZA ILKA MARIA FERNANdeS SOARES TEREZINHA MARIA CASTRO deLLA LUCIA Institut de Recherche sur la Biologie de 1'Insecte Université Francois Rabelais Tours France departamento de biologia Animal Laboratório de Ecologia de Comunidades Departamento de Biologia Geral Universidade Federal de Vicosa MG 36570-000 Brazil

The fungus-growing ants （Tribe Attini） are a New World group of〉 200 species, all obligate symbionts with a fungus they use for food. Four attine taxa are known to be social parasites of other attines. Acromyrmex （ Pseudoatta） argentina argentina and Acromyrmex （Pseudoatta） argentina platensis （parasites of Acromyrmex lundi）, and Acromyrmex sp. （a parasite of Acromyrmex rugosus） produce no worker caste. In contrast, the recently discovered Acromyrmex insinuator （a parasite of Acromyrmex echinatior） does produce workers. Here, we describe a new species, Acromyrmex ameliae, a social parasite of Acromyrmex subterraneus subterraneus and Acromyrmex subterraneus brunneus in Minas Gerais, Brasil. Like A. insinuator, it produces workers and appears to be closely related to its hosts. Similar social parasites may be fairly common in the fungus-growing ants, but overlooked due to the close resemblance between parasite and host workers.

关键词： Acromyrmex leaf-cutting ants social evolution social parasitism

Vascular endothelial growth factor：an essential neurotrophic factor for motoneurons？

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Neural Regeneration Research 2018年第7期13卷 1181-1182页

作者： Paula M.Calvo Angel M.Pastor Rosa R.de la Cruz departamento de Fisiologia Facultad de Biologia Universidad de Sevilla Sevilla Spain

Vascular endothelial growth factor（VEGF）,an angiogenic factor with neuroprotective effects：The VEGF was initially characterized by its vasculogenic and angiogenic activities and its capacity to promote vascular permeability（yancopoulos et al.,2000）.VEGF is also known as VEGF-A and is the prototype member of a related group of five trophic factors,VEGF-B,VEGF-C,VEGF-D and placental growth factor（Pl GF;Lange et al.,2016）.

关键词： VEGFR Vascular endothelial growth factor:an essential neurotrophic factor for motoneurons