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Disruption of a microvitellogenin gene impairs eggshell formation in Mythimna separata

Journal of Integrative Agriculture 2024年第11期 3801-3811页

作者： Lingling Li Junhong Fu Changgeng Dai Yuhang Zhou Yang Hu Hongbo Li Institute of Plant Protection Guizhou Academy of Agricultural Sciences Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous region School of Agriculture Institute of Entomology Guizhou University crop genetic resources and germplasm innovation in karst region Ministry of Agriculture and Rural Affairs

Microvitellogenin(mVg) belongs to the lepidopteran-specific lipoprotein＿11 super family and is thought to be involved in insect reproduction. However, the function of mVg in reproduction has not been established. In this study, we identified and characterized MsmVg in Mythimna separata, a destructive insect pest of grain crops. MsmVg is comprised of a 1,310 bp open reading frame(ORF) encoding 437 amino acids. Structural analysis showed that MsmVg contains three conserved domains in the lipoprotein＿11 super family, including the all-α-N-terminal domain(NTD), a Pseudomonas putida homologous domain(PPD), and an all-β-C-terminal domain(CTD). MsmVg was highly expressed in mature female adults and enriched in the ovaries of 3-day-old female adults. Disruption of MsmVg by RNA interference did not change the expression of MsmVg, vitellogenin(MsVg) or the vitellogenin receptor(MsVgR) in fat bodies, but it inhibited their transcription in ovaries. Phenotype analysis showed that knockdown of MsmVg did not affect yolk deposition in ovaries, but impaired eggshell formation and led to a reduction in the reproductive capacity. Furthermore, knockdown of MsmVg significantly inhibited the expression of genes associated with eggshell formation(MsChP1-3 and MsFcP3C). Taken together, these data suggest that MsmVg plays an important role in female reproduction by affecting eggshell formation in M. separata, which provides a potential target for managing this insect pest.

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Evaluation of Resistance of Different Kiwifruit Varieties (Lines) to Canker Disease and Brown Spot Disease

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1249-1261页

作者： Wenwen Su Chongpei Zheng Zhencheng Han Chunguang Ren Di Wu Tao Li Yi Yang Weijie Li Institute of Mountain resources of Guizhou Province Guizhou Academy of SciencesGuiyang550001China Guizhou Botanical Garden Guizhou Academy of SciencesGuiyang550001China Key Laboratory of Plant Resource Conservation and germplasm innovation in Mountainous region(Ministry of Education) Institute of Agro-Bioengineering/College of Life SciencesGuizhou UniversityGuiyang550025China

Kiwifruit canker and brown spot are signiﬁcant diseases affecting kiwis,caused by Pseudomonas syringae patho-genic variations(Pseudomonas syringae ***(Psa))and Corynesporapolytica(Corynespora cassiicola).At present,the research on canker disease and brown spot disease mainly focuses on the isolation and identiﬁcation of pathogenic bacteria,drug control,resistance gene mining and functional veriﬁ*** has proved that breeding disease resistant varieties are an effective method to control canker disease and brown spot ***,most existing cultivars lack genes for canker and brown spot *** kiwifruit resources in nat-ure exhibit extensive genetic diversity due to prolonged natural selection,containing numerous resistance ***,due to insufﬁcient understanding of the resistance of most kiwifruit varieties(lines)to canker disease and brown spot disease,some high-quality resources have not been fully *** incidence of canker and brown spot of 18 kiwifruit cultivars(lines)was measured by inoculating isolated branches and leaves,and their resistance to canker and brown spot was analyzed according to the length,disease index,mean diameter,and systematic *** results were as follows:Among 18 different kiwifruit varieties(lines)for canker disease,there were two highly resistant materials,eight disease-resistant materials,four disease-susceptible materials,and two highly susceptible ***,regarding brown spot disease,there were one highly resistant material,ﬁve dis-ease-resistant materials,four susceptible materials,and three highly susceptible ***,four resources were resistant to both *** outcomes provided a theoretical basis for breeding kiwifruit against canker and brown spot.

关键词： Kiwifruit canker disease brown spot resistance evaluation

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GmAP1d regulates flowering time under long-day photoperiods in soybean

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The crop Journal 2024年第3期12卷 845-855页

作者： Shiyu Guo Yanfei Li Hongmei Qiu Guoyu Hu Chaosen Zhao Ruizhen Wang Hao Zhang Yu Tian Xiaoyu Li Bin Liu Ying-hui Li Li-juan Qiu College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina State Key Laboratory of crop Gene resources and Breeding/The National Key Facility for crop Gene resources and genetic Improvement(NFCRI)/Key Laboratory of Grain crop genetic resources Evaluation and Utilization(MARA) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Jilin Academy of Agricultural Sciences/National Engineering Research Center for Soybean Changchun 130033JilinChina crop Research Institute Anhui Academy of Agricultural SciencesHefei 230031AnhuiChina crops Research Institute of Jiangxi Academy of Agricultural Sciences/Jiangxi Province Key Laboratory of Oilcrops Biology Nanchang 330200JiangxiChina Key Lab of Chinese Medicine resources Conservation State Administration of Traditional Chinese Medicine of the People’s Republic of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China

Flowering time is important for adaptation of soybean(Glycine max)to different ***,we conducted a genome-wide association study of flowering time using a panel of 1490 cultivated soybean *** identified three strong signals at the qFT02-2 locus(Chr02:12037319–12238569),which were associated with flowering time in three environments:Gongzhuling,Mengcheng,and *** analyzing linkage disequilibrium,gene expression patterns,gene annotation,and the diversity of variants,we identified an AP1 homolog as the candidate gene for the qFT02-2 locus,which we named *** one nonsynonymous polymorphism existed among 1490 soybean accessions at position Chr02:*** carrying the Chr02:12087053-T allele flowered significantly earlier than those carrying the Chr02:12087053-A ***,we developed a cleaved amplified polymorphic sequence(CAPS)marker for the SNP at Chr02:12087053,which is suitable for marker-assisted breeding of flowering *** of GmAP1d in the‘Williams 82’background by gene editing promoted flowering under long-day conditions,confirming that GmAP1d is the causal gene for *** analysis of the region surrounding GmAP1d revealed that GmAP1d was artificially selected during the genetic improvement of *** stepwise selection,the proportion of modern cultivars carrying the Chr02:12087053-T allele has increased,and this allele has become nearly fixed(95%)in northern *** findings provide a theoretical basis for better understanding the molecular regulatory mechanism of flowering time in soybean and a target gene that can be used for breeding modern soybean cultivars adapted to different latitudes.

关键词： Soybean Flowering time GWAS GmAP1d Long-day conditions

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Candidate genes conferring ethylene-response in cultivated peanuts determined by BSA-seq and fine-mapping

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The crop Journal 2024年第3期12卷 856-865页

作者： Yanyan Tang Zhong Huang Shaohui Xu Wenjie Zhou Jianjun Ren Fuxin Yu Jingshan Wang Wujun Ma Lixian Qiao College of Agronomy Qingdao Agricultural UniversityQingdao 266109ShandongChina Murdoch University Food Futures InstitutePerthWA 6150Australia The Characteristic Laboratory of crop germplasm innovation and Application Provincial Department of EducationCollege of AgronomyQingdao Agricultural UniversityQingdao 266109ShandongChina Academy of Dongying Efficient Agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with Qingdao Agricultural University Dongying 257347ShandongChina

Ethylene plays essential roles in plant growth,development and stress *** ethylene signaling pathway and molecular mechanism have been studied extensively in Arabidopsis and rice but limited in ***,we established a sand-culture method to screen pingyangmycin mutagenized peanut lines based on their specific response to ethylene(“triple response”).An ethylene-insensitive mutant,inhibition of peanut hypocotyl elongation 1(iph1),was identified that showed reduced sensitivity to ethylene in both hypocotyl elongation and root *** bulked segregant analysis sequencing,a major gene related to iph1,named AhIPH1,was preliminarily mapped at the chromosome Arahy.01,and further narrowed to a 450-kb genomic region through substitution mapping strategy.A total of 7014 genes were differentially expressed among the ACC treatment through RNA-seq analysis,of which only the Arahy.5BLU0Q gene in the candidate mapping interval was differentially expressed between WT and mutant *** sequence variations,functional annotation and transcriptome analysis revealed that a predicated gene,Arahy.5BLU0Q,encoding SNF1 protein kinase,may be the candidate gene for *** gene contained two single-nucleotide polymorphisms at promoter region and was more highly expressed in iph1 than *** findings reveal a novel ethylene-responsive gene,which provides a theoretical foundation and new genetic resources for the mechanism of ethylene signaling in peanuts.

关键词： Ethylene-insensitive Hypocotyl elongation AhIPH1 Candidate gene genetic resources

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A centromere map based on super pan-genome highlights the structure and function of rice centromeres

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Journal of Integrative Plant Biology 2024年第2期66卷 196-207页

作者： Yang Lv Congcong Liu Xiaoxia Li Yueying Wang Huiying He Wenchuang He Wu Chen Longbo Yang Xiaofan Dai Xinglan Cao Xiaoman Yu Jiajia Liu Bin Zhang Hua Wei Hong Zhang Hongge Qian Chuanlin Shi Yue Leng Xiangpei Liu Mingliang Guo Xianmeng Wang Zhipeng Zhang Tianyi Wang Bintao Zhang Qiang Xu Yan Cui Qianqian Zhang Qiaoling Yuan Noushin Jahan Jie Ma Xiaoming Zheng Yongfeng Zhou Qian Qian Longbiao Guo Lianguang Shang State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Yazhouwan National Laboratory No.8 Huanjin RoadYazhou DistrictSanya City 572024China National Key Facility for crop Gene resources and genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China

Rice(Oryza sativa)is a significant crop worldwide with a genome shaped by various evolutionary *** centromeres are crucial for chromosome segregation,and contain some unreported *** to the diverse and complex centromere region,a comprehensive understanding of rice centromere structure and function at the population level is *** constructed a high-quality centromere map based on the rice super pangenome consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African *** showed that rice centromeres have diverse satellite repeat CentO,which vary across chromosomes and subpopulations,reflecting their distinct evolutionary *** also revealed that long terminal repeats(LTRs),especially young Gypsy-type LTRs,are abundant in the peripheral CentO-enriched regions and drive rice centromere expansion and ***,high-quality genome assembly and complete telomere-to-telomere(T2T)reference genome enable us to obtain more centromeric genome information despite mapping and cloning of centromere genes being *** investigated the association between structural variations and gene expression in the rice centromere.A centromere gene,OsMAB,which positively regulates rice tiller number,was further confirmed by expression quantitative trait loci,haplotype analysis and clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein9 *** revealing the new insights into the evolutionary patterns and biological roles of rice centromeres,our finding will facilitate future research on centromere biology and crop improvement.

关键词： centromere super pan-genome CentoO satellite repeat rice

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Novel and favorable genomic regions for spike related traits in a wheat germplasm Pubing 3504 with high grain number per spike under varying environments

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Journal of Integrative Agriculture 2017年第11期16卷 2386-2401页

作者： CHEN Dan WU Xiao-yang WU Kuo ZHANG Jin-peng LIU Wei-hua YANG Xin-ming LI Xiu-quan LU Yu-qing LI Li-hui National Key Facility for crop Gene resources and genetic Improvement/Institute of crop Sciences Chinese Academy of Agricultural Sciences Biotechnology and germplasm resources Institute Yunnan Academy of Agricultural Sciences/Key Laboratory of Southwestern Crop Gene Resources and Germplasm InnovationMinistry of Agriculture/Scientific Observation for Rice Germplasm Resources of YunnanMinistry of Agriculture/Yunnan Provincial Key Laboratory of Agricultural Biotechnology

Grain number per spike （GNPS） is a major factor in wheat yield breeding. A new wheat germplasm Pubing 3504 shows superior features in spike traits. To elucidate the genetic basis of spike and yield related traits in Pubing 3504, 282 F2：3 families were generated from the cross Pubing 3504xJing 4839, and seven spike and yield related traits, including GNPS, spike length （SL）, kernel number per spikelet （KPS）, spikelet number per spike （SNS）, thousand-grain weight （TGW）, spike number per plant （SNP）, and plant height （HT） were investigated. Correlation analysis indicated significant positive correlations between GNPS and spike-related traits, including KPS, SNS, and SL, especially KPS. A genetic map was constructed using 190 polymorphic simple sequence repeat （SSR）, expressed sequence tag （EST）-SSR, and sequence- tagged-site （STS） markers. For the seven traits measured, a total of 37 quantitative trait loci （QTLs） in a single-environment analysis and 25 QTLs in a joint-environment analysis were detected. Additive effects of 70.3% （in a single environment） and 57.6% （in a joint environment） of the QTLs were positively contributed by Pubing 3504 alleles. Five important genomic regions on chromosomes 1A, 4A, 4B, 2D, and 4D could be stably detected in different environments. Among these regions, the marker interval Xmag834-Xbarc83 on the short arm of chromosome 1A was a novel important genomic region that included QTLs controlling GNPS, KPS, SNS, TGW, and SNP with stable environmental repeatability. This genomic region can improve the spike trait and may play a key role in improving wheat yield in the future. We deduced that this genomic region was vital to the high GNPS of Pubing 3504.

关键词： wheat high GNPS germplasm QTL mapping genomic region

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Characterization of a 4.1 Mb inversion harboring the stripe rust resistance gene YR86 on wheat chromosome 2AL

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The crop Journal 2024年第4期12卷 1168-1175页

作者： Qiang Cao Zhanwang Zhu Dengan Xu Jianhui Wu Xiaowan Xu Yan Dong Yingjie Bian Fugong Ding Dehui Zhao Yang Tu Ling Wu Dejun Han Caixia Lan Xianchun Xia Zhonghu He Yuanfeng Hao College of Agronomy Northwest A&F UniversityYangling 712100ShaanxiChina State Key Laboratory of crop Gene resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural Sciences(CAAS)Beijing 100081China Food crops Institute Hubei Academy of Agricultural Sciences/Wheat Disease Biology Research Station for Central ChinaWuhan 430064HubeiChina crop Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066SichuanChina Hubei Hongshan Laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina CIMMYT-China Office C/O CAASBeijing 100081China

Wheat cultivar Zhongmai 895 was earlier found to carry YR86 in an 11.6 Mb recombination-suppressed region on chromosome 2AL when crossed with Yangmai *** fine-map the YR86 locus,we developed two large F2 populations from crosses Emai 580/Zhongmai 895 and Avocet S/Zhongmai ***,both populations exhibited suppressed recombination in the same 2AL *** analysis across Chinese Spring,Aikang 58,and 10+wheat genomes revealed a 4.1 Mb chromosomal inversion spanning 708.5-712.6 Mb in the Chinese Spring reference *** markers were developed in the breakpoint and were used to assess a wheat cultivar panel,revealing that Chinese Spring,Zhongmai 895,and Jimai 22 shared a common sequence named InvCS,whereas Aikang 58,Yangmai 16,Emai 580,and Avocet S shared the sequence named *** inverted configuration explained the suppressed recombination observed in all three bi-parental *** recombination was observed in a Jimai 22/Zhongmai 895 F2 population,facilitating mapping of YR86 to a genetic interval of 0.15 cM corresponding to 710.27-712.56 Mb falling within the inverted ***-three high-confidence genes were annotated in the interval using the Chinese Spring reference genome,with six identified as potential candidates for YR86 based on genome and transcriptome *** results will accelerate map-based cloning of YR86 and its deployment in wheat breeding.

关键词： Adult-plant resistance Chromosomal inversion Puccinia striiformis Triticum aestivum

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Mapping and validation of a dominant salt tolerance gene in the cultivated soybean(Glycine max) variety Tiefeng 8

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The crop Journal 2014年第6期2卷 358-365页

作者： Rongxia Guan Jiangang Chen Jinghan Jiang Guangyu Liu Ying Liu Lei Tian Lili Yu Ruzhen Chang Li-juan Qiu The National Key Facility for crop Gene resources and genetic Improvement (NFCRI) Key Laboratory of Germplasm & Biotechnology (MOA)Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Salt is an abiotic stress factor that strongly affects soybean growth and production. A single dominant gene has been shown to confer salt tolerance in the soybean cultivar Tiefeng *** objective of the present study was to genetically map the salt-tolerance gene in an F2:3population and a recombinant inbred line(RIL) population derived from a cross between two cultivated soybeans, Tiefeng 8(tolerant) and 85-140(sensitive). The F2:3families and RILs were treated with 200 mmol L-1Na Cl to evaluate salt tolerance. The F2:3population showed 1(42 tolerant): 2(132 segregating): 1(65 sensitive) segregation, indicating a single dominant gene for salt tolerance in Tiefeng 8. A sequence-characterized amplified region(SCAR) marker from a previously identified random amplified polymorphic DNA(RAPD)marker and four insertion/deletion polymorphism(In Del) markers were developed within the mapping region. Using these markers along with SSR markers, the salt-tolerance gene was mapped within 209 kb flanked by SCAR marker QS08064 and SSR marker Barcsoyssr_3_1301 on chromosome 3. Three markers that cosegregated with the salt tolerance gene and SCAR marker QS08064 were used to genotype 35 tolerant and 23 sensitive soybean accessions. These markers showed selection efficiencies of 76.2% to94.2%. The results indicate that these markers will be useful for marker-assisted breeding and facilitating map-based cloning of the salt tolerance gene in soybean.

关键词： Salt tolerance Gene mapping Soybean Glycine max

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QTL underlying iron and zinc toxicity tolerances at seedling stage revealed by two sets of reciprocal introgression populations of rice(Oryza sativa L.)

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The crop Journal 2016年第4期4卷 280-289页

作者： Huan Liu Aijaz Soomro Yajun Zhu Xianjin Qiu Kai Chen Tianqing Zheng Longwei Yang Danying Xing Jianlong Xu Hubei Collaborative innovation Center for Grain Industry Yangtze University Institute of crop Science/National Key Facility for crop Gene resources and genetic Improvement Chinese Academy of Agricultural Sciences Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen Institute of Breeding for innovation Chinese Academy of Agricultural Sciences

Iron and zinc are two trace elements that are essential for rice. But they are toxic at higher concentrations, leading to severe rice yield losses especially in acid soils and inland valleys. In this study, two reciprocal introgression line(IL) populations sharing the same parents were used with high-density SNP bin markers to identify QTL tolerant to iron and zinc toxicities. The results indicated that the japonica variety 02,428 had stronger tolerance to iron and zinc toxicities than the indica variety Minghui 63. Nine and ten QTL contributing to iron and zinc toxicity tolerances,respectively, were identified in the two IL populations. The favorable alleles of most QTL came from 02,428. Among them, q FRRDW2, q ZRRDW3, and q FRSDW11 appeared to be independent of genetic background. The region C11S49–C11S60 on chromosome 11 harbored QTL affecting multiple iron and zinc toxicity tolerance-related traits, indicating partial genetic overlap between the two toxicity tolerances. Our results provide essential information and materials for developing excellent rice cultivars with iron and/or zinc tolerance by marker-assisted selection(MAS).

关键词： Rice Reciprocal introgression lines Iron tolerance Zinc tolerance Quantitative trait locus/loci(QTL)

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OsPKpa_1 encodes a plastidic pyruvate kinase that affects starch biosynthesis in the rice endosperm

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Journal of Integrative Plant Biology 2018年第11期60卷 1097-1118页

作者： Yue Cai Wenwei Zhang Jie Jin Xiaoming Yang Xiaoman You Haigang Yan Liang Wang Jie Chen Jiahuan Xu Weiwei Chen Xingang Chen Jing Ma Xiaojie Tang Fei Kong Xiaopin Zhu Guoxiang Wang Ling Jiang William Terzaghi Chunming Wang Jianmin Wan State Key Laboratory for crop genetics and germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing AgriculturalUniversityNanjing 210095China National Key Facility for crop Gene resources and genetic Improvement Institute of Crop ScienceChinese Academy of AgriculturalSciencesBeijing 100081China Department of Biology Wilkes UniversityWilkes-BarrePA 18766USA

Pyruvate kinase （PK） is a key enzyme in glycolysis and carbon metabolism. Here, we isolated a rice （Oryza sativa） mutant, w59, with a white-core floury endosperm. Map-based cloning of w59 identified a mutation in OsPKpα1, which encodes a plastidic isoform of PK （PKp）. OsPKpα1 localizes to the amyloplast stroma in the developing endosperm, and the mutation of OsPKpα1 in w59 decreases the plastidic PK activity, resulting in dramatic changes to the lipid biosynthesis in seeds. The w59 grains were also characterized by a marked decrease in starch content. Consistent with a decrease in number and size of the w59 amyloplasts, large empty spaces were observed in the central region of the w59 endosperm, at the early grain-filling stage. Moreover, a phylogenetic analysis revealed four potential rice isoforms of OsPKp. We validated the in vitro PK activity of these OsPKps through reconstituting active PKp complexes derived from inactive individual OsPKps, revealing the heteromeric structure of rice PKps, which was further confirmed using a protein- protein interaction analysis. These findings suggest a functional connection between lipid and starch synthesis in rice endosperm amyloplasts.

关键词： Figure OsPKpa1 encodes a plastidic pyruvate kinase that affects starch biosynthesis in the rice endosperm

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