Disruption of a microvitellogenin gene impairs eggshell formation in Mythimna separata
作者机构：Institute of Plant Protection Guizhou Academy of Agricultural Sciences Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region School of Agriculture Institute of Entomology Guizhou University Crop Genetic Resources and Germplasm Innovation in Karst Region Ministry of Agriculture and Rural Affairs
出 版 物：《Journal of Integrative Agriculture》 (农业科学学报(英文版))
年 卷 期：2024年第11期
页 面：3801-3811页
核心收录：
学科分类：09[农学] 0904[农学-植物保护] 090402[农学-农业昆虫与害虫防治]
基 金：funded by the Science and Technology Program of Guizhou Province, China (IZ022) the National Natural Science Foundation of China (32160641) the Science & Technology Support Program of Guizhou Province, China (2023–055) the Training Program of Guizhou University, China (32 and 45)
摘 要：Microvitellogenin(mVg) belongs to the lepidopteran-specific lipoprotein＿11 super family and is thought to be involved in insect reproduction. However, the function of mVg in reproduction has not been established. In this study, we identified and characterized MsmVg in Mythimna separata, a destructive insect pest of grain crops. MsmVg is comprised of a 1,310 bp open reading frame(ORF) encoding 437 amino acids. Structural analysis showed that MsmVg contains three conserved domains in the lipoprotein＿11 super family, including the all-α-N-terminal domain(NTD), a Pseudomonas putida homologous domain(PPD), and an all-β-C-terminal domain(CTD). MsmVg was highly expressed in mature female adults and enriched in the ovaries of 3-day-old female adults. Disruption of MsmVg by RNA interference did not change the expression of MsmVg, vitellogenin(MsVg) or the vitellogenin receptor(MsVgR) in fat bodies, but it inhibited their transcription in ovaries. Phenotype analysis showed that knockdown of MsmVg did not affect yolk deposition in ovaries, but impaired eggshell formation and led to a reduction in the reproductive capacity. Furthermore, knockdown of MsmVg significantly inhibited the expression of genes associated with eggshell formation(MsChP1-3 and MsFcP3C). Taken together, these data suggest that MsmVg plays an important role in female reproduction by affecting eggshell formation in M. separata, which provides a potential target for managing this insect pest.
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