检索结果-南通市图书馆

Overexpression of OsPAP10a, A Root-Associated Acid Phosphatase, Increased Extracellular Organic Phosphorus Utilization in Rice

引用

Journal of Integrative Plant biology 2012年第9期54卷 631-639页

作者： Jingluan Tian Chuang Wang Qian Zhang Xiaowei He James Whelan Huixia Shou State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310058China joint Research Laboratory in genomics and Nutriomics College of Life SciencesZhejiang UniversityHangzhou 310058China present address:Guangdong Academy of Forestry Guangzhou 510520China Australian Research council Centre of Excellence in Plant Energy biology University of Western AustraliaCrawley 6009WAAustralia

Phosphorus （P） deficiency is a major limitation for plant growth and development. Among the wide set of responses to cope with low soil P, plants increase their level of intracellular and secreted acid phosphatases （APases）, which helps to catalyze inorganic phosphate （Pi） hydrolysis from organophosphates, in this study we characterized the rice （Oryza sativa） purple acid phosphatase 10a （OsPAPIOa）. OsPAPIOa belongs to group la of purple acid phosphatases （PAPs）, and clusters with the principal secreted PAPs in a variety of plant species including Arabidopsis. The transcript abundance of OsPAPIOa is specifically induced by Pi deficiency and is controlled by OsPHR2, the central transcription factor controlling Pi homeostasis. In gel activity assays of root and shoot protein extracts, it was revealed that OsPAPIOa is a major acid phosphatase isoform induced by Pi starvation. Constitutive overexpression of OsPAPIOa results in a significant increase of phosphatase activity in both shoot and root protein extracts. In vivo root 5-bromo.4-chloro-3-indolyl-phosphate （BCIP） assays and activity measurements on external media showed that OsPAPIOa is a root-associated APase. Furthermore, overexpression of OsPAPIOa significantly improved ATP hydrolysis and utilization compared with wild type plants. These results indicate that OsPAPIOa can potentially be used for crop breeding to improve the efficiency of P use.

关键词： Purple acid phosphatase phosphate rice OsPAPIOa root-associated APase.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Filamentation of Metabolic Enzymes in Saccharomyces cerevisiae

引用

Journal of Genetics and genomics 2016年第6期43卷 393-404页

作者： Qing-Ji Shen Hakimi Kassim Yong Huang Hui Li Jing Zhang Guang Li Peng-Ye Wang Jun Yan Fangfu Ye Ji-Long Liu Medical Research council functional genomics Unit Department of PhysiologyAnatomy and GeneticsUniversity of Oxford Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest University Key Laboratory of Soft Matter Physics Beijing National Laboratory for Condensed Matter PhysicsInstitute of PhysicsChinese Academy of Sciences CAS-MPG Partner Institute for Computational biology Shanghai Institutes of Biological SciencesChinese Academy of Sciences

Compartmentation via filamentation has recently emerged as a novel mechanism for metabolic regulation. In order to identify filamentforming metabolic enzymes systematically, we performed a genome-wide screening of all strains available from an open reading frameGFP collection in Saccharomyces cerevisiae. We discovered nine novel filament-forming proteins and also confirmed those identified previously. From the 4159 strains, we found 23 proteins, mostly metabolic enzymes, which are capable of forming filaments in vivo. In silico protein-protein interaction analysis suggests that these filament-forming proteins can be clustered into several groups, including translational initiation machinery and glucose and nitrogen metabolic pathways. Using glutamine-utilising enzymes as examples, we found that the culture conditions affect the occurrence and length of the metabolic filaments. Furthermore, we found that two CTP synthases（Ura7p and Ura8p） and two asparagine synthetases（Asn1p and Asn2p） form filaments both in the cytoplasm and in the *** imaging analyses suggest that metabolic filaments undergo sub-diffusion. Taken together, our genome-wide screening identifies additional filament-forming proteins in S. cerevisiae and suggests that filamentation of metabolic enzymes is more general than currently appreciated.

关键词： CTP synthase Metabolic enzyme Cytoophidium Glycolysis Glutamine Intracellular compartmentation Saccharomyces cerevisiae

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cancer-associated fibroblasts express CD1d and activate invariant natural killer T cells under cellular stress

引用

Cellular & Molecular Immunology 2024年第1期21卷 91-94页

作者： Shengduo Pei Jonas Sjölund Yueyun Pan Kristian Pietras Mikael C.I.Karlsson Department of Microbiology Tumor and Cell BiologyKarolinska InstitutetStockholmSweden Division of translational Cancer Research Department of Laboratory MedicineLund University Cancer CentreLund UniversityLundSweden

Invariant natural killer T(iNKT)cells are part of the family of unconventional T cells,and they recognize glycolipid antigens presented on the MHC class I like molecule CD1^([1]).In humans there are five CD1 molecules named CD1a to CD1e,whereas mice use only CD1d for lipid ***,iNKT cells can elicit both cytotoxicity similarly to conventional CD8 T cells and secrete cytokines that influence the adaptive immune response including providing direct T cell help to B cells^([2]).In the context of tumors,they can use both release of granzyme B and use Fas-mediated mechanisms for killing of tumor *** iNKT cells use a restricted TCR repertoire and CD1 molecules are non-heterogenous the risk of graft vs host disease is limited and thus these T cells have been an attractive option for off the shelf immunotherapy to treat cancer^([3]).

关键词： killer CD1d Cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

WDR74 functions as a novel coactivator in TGF-β signaling

引用

Journal of Genetics and genomics 2018年第12期45卷 639-650页

作者： Jinquan Liu Meiling Zhao Bo Yuan Shuchen Gu Mingjie Zheng Jian Zou Jianping Jin Ting Liu Xin-Hua Feng MOE Key Laboratory of Biosystems Homeostasis & Protection and Innovation Center for Cell Signaling Network Life Sciences Institute Zhejiang University Department of Biochemistry and Molecular biology University of Texas Health Science Center at Houston Institute of translational Medicine School of Medicine Zhejiang University Department of Cell biology School of Medicine Zhejiang University Department of Molecular & Cellular biology and Michael DeBakey Department of Surgery Baylor College of Medicine

Smads are critical intracellular signal transducers for transforming growth factor-β(TGF-β) in mammalian cells. In this study, we have identified WD repeat-containing protein 74(WDR74) as a novel transcriptional coactivator for Smads in the canonical TGF-β signaling pathway. Through direct interactions with Smad proteins, WDR74 enhances TGF-β-mediated phosphorylation and nuclear accumulation of Smad2 and Smad3. Consequently, WDR74 enables stronger transcriptional responses and more robust TGF-β-induced physiological responses. Our findings have elucidated a critical role of WDR74 in regulating TGF-β signaling.

关键词： WDR74 TGF-β signaling Transcription Growth inhibition EMT

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Generation of Hematopoietic Stem Cells from Purified Embryonic Endothelial Cells by a Simple and Efficient Strategy

引用

Journal of Genetics and genomics 2013年第11期40卷 557-563页

作者： Zhuan Li Fan Zhou Dongbo Chen Wenyan He Yanli Ni Lingfei Luo Bing Liu Key Laboratory of Freshwater Fish Reproduction and Development Ministry of EducationLaboratory of Molecular Developmental BiologySchool of Life ScienceSouthwest University 307-Ivy translational Medicine Center Laboratory of OncologyAffiliated Hospital of Academy of Military Medical Sciences

Recent progress by versatile approaches supports the new hypothesis that multi-potent hematopoietic stein cells （HSCs） are directly formed from a rare population of endothelial cells in mid-gestation mouse embryos. This process is therefore known as the endothelial-to- hematopoietic transition （EHT）. Nevertheless, there is no functional evidence that documents the HSC transition from purified endothelial cells. In this study, we developed an OP9-DLl-based co-culture system that was able to facilitate the HSC specification and/or expansion in vitro of mouse embryonic day 10.5 （El0.5） Tie2~ cells remarkably. Then, the immunophenotypically defined endothelial ceils were harvested by a combination of surface markers （Flkl＋CD31 ~CD41 CD45 Ter119 ） from the caudal half of EI0.0-EI 1.0 mouse embryos. The transplantation of the endothelia/OP9-DL1 co-cultures led to long-term, high-level, multi-lineage, and multi-organ he- matopoietic reconstitution in the irradiated adult recipients. The induced HSC activity was initially observed at El0.5, and a significant increase was detected at El 1.0, which suggests a temporally specific regulation. Taken together, tbr the first time, we provide functional evidence showing the HSC potential of purified embryonic endothelial cells, which is indispensable for the emerging EHT concept. Moreover, the newly defined co-culture system will aid the exploration of the key molecules governing the HSC transition from embryonic and even postnatal endothelial cells, which has enormous significance in basic and translational research.

关键词： Hematopoietic stem cells Endothelial-to-hematopoietic transition Endothelial cells Aorta-gonad--mesonephros region

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Palmitoylation of GNAQ/11 is critical for tumor cell proliferation and survival in GNAQ/11-mutant uveal melanoma

引用

Frontiers of Medicine 2022年第5期16卷 784-798页

作者： Yan Zhang Baoyuan Zhang Yongyun Li Yuting Dai Jiaoyang Li Donghe Li Zhizhou Xia Jianming Zhang Ping Liu Ming Chen Bo Jiao Ruibao Ren Shanghai Institute of Hematology State Key Laboratory of Medical GenomicsNational Research Center for Translational Medicine at ShanghaiCollaborative Innovation Center of HematologyRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of Ophthalmology Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai Key Laboratory of Orbital Diseases and Ocular OncologyShanghai 200001China Department of biology Brandeis UniversityWalthamMA 02454USA

More than 85%of patients with uveal melanoma(UM)carry a GNAQ or GNA11 mutation at a hotspot codon(Q209)that encodes G proteinαsubunit q/11 polypeptides(Gα_(q/11)).GNAQ/11 relies on palmitoylation for membrane association and signal *** the palmitoylation of GNAQ/11 was discovered long before,its implication in UM remains ***,results of palmitoylation-targeted mutagenesis and chemical interference approaches revealed that the loss of GNAQ/11 palmitoylation substantially affected tumor cell proliferation and survival in UM *** inhibition through the mutation of palmitoylation sites suppressed GNAQ/11^(Q209L)-induced malignant transformation in NIH3T3 ***,the palmitoylation-deficient oncogenic GNAQ/11 failed to rescue the cell death initiated by the knock down of endogenous GNAQ/11 oncogenes in UM cells,which are much more dependent on Gα_(q/11) signaling for cell survival and proliferation than other melanoma cells without GNAQ/11 ***,the palmitoylation inhibitor,2-bromopalmitate,also specifically disrupted Gα_(q/11) downstream signaling by interfering with the MAPK pathway and BCL2 survival pathway in GNAQ/11-mutant UM cells and showed a notable synergistic effect when applied in combination with the BCL2 inhibitor,ABT-199,in *** findings validate that GNAQ/11 palmitoylation plays a critical role in UM and may serve as a promising therapeutic target for GNAQ/11-driven UM.

关键词： uveal melanoma mutant GNAQ/11 palmitoylation BCL2 combination target therapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

translational Informatics for Parkinson’s Disease:from Big Biomedical Data to Small Actionable Alterations

引用

genomics, Proteomics & Bioinformatics 2019年第4期17卷 415-429页

作者： Bairong Shen Yuxin Lin Cheng Bi Shengrong Zhou Zhongchen Bai Guangmin Zheng Jing Zhou Institutes for Systems Genetics West China HospitalSichuan UniversityChengdu 610041China Center for Systems biology Soochow UniversitySuzhou 215006China Center for translational Biomedical Informatics Guizhou University School of MedicineGuiyang 550025China

Parkinson’s disease(PD)is a common neurological disease in elderly people,and its morbidity and mortality are increasing with the advent of global *** traditional paradigm of moving from small data to big data in biomedical research is shifting toward big data-based identification of small actionable *** highlight the use of big data for precision PD medicine,we review PD big data and informatics for the translation of basic PD research to clinical *** emphasize some key findings in clinically actionable changes,such as susceptibility genetic variations for PD risk population screening,biomarkers for the diagnosis and stratification of PD patients,risk factors for PD,and lifestyles for the prevention of *** challenges associated with the collection,storage,and modelling of diverse big data for PD precision medicine and healthcare are also *** perspectives on systems modelling and intelligent medicine for PD monitoring,diagnosis,treatment,and healthcare are discussed in the end.

关键词： Parkinson’s disease Healthcare Disease biomarker translational informatics Systems modelling

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Exome sequencing confirms molecular diagnoses in 38 Chinese families with hereditary spherocytosis

引用

Science China(Life Sciences) 2018年第8期61卷 947-953页

作者： Rongrong Wang Shuanghao Yang Ming Xu Jia Huang Hongyan Liu Weiyue Gu Xue Zhang Mc Kusick-Zhang Center for Genetic Medicine State Key Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College Joy Orient translational Medicine Research Center Co. Ltd The Research Center for Medical genomics China Medical University Medical Genetics Institute Henan Provincial People's Hospital People's Hospital of Zhengzhou University People's Hospital of Henan University

Hereditary spherocytosis （HS）, the most common cause of congenital hemolytic anemia, is caused by deficiency of the ery- throcyte membrane proteins. Five causative genes （ANK1, SPTB, SPTA1, SLC4AI, and EPB42） have been identified. To date, molecular genetic studies have been performed in different populations, including the American, European, Brazilian, Japanese and Korean populations, whereas only a few studies have been described in the Chinese population. Here, by reanalysis of the exome data, we revealed causative mutations and established a definitive diagnosis of HS in all 38 Chinese families. We found 34 novel mutations and four reported mutations in three known HS-causing genes--17 in ANK1, 17 in SPTB and four in SLC4A1, suggesting that ANK1 and SPTB are the major genes in Chinese patients with HS. All of the ANK1 or SPTB mutations, scattered throughout the entire genes, are non-recurrent; and most of them are null mutations, which might cause HS via a hap-loinsufficiency mechanism. De novo mutations in ANK1 or SPTB often occur with an unexpected high frequency （87.5% and 64.2%, respectively）. Our study updates our knowledge about the genetic profile of HS in Chinese and shows that family-based, especially parent-offspring trio, sequencing analysis can help to increase the diagnostic power and improve diagnostic efficiency.

关键词： hereditary spherocytosis mutation ANK1 SPTB SLC4A1 whole-exome sequencing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phase separation of GRP7 facilitated by FERONIAmediated phosphorylation inhibits mRNA translation to modulate plant temperature resilience

引用

Molecular Plant 2024年第3期17卷 460-477页

作者： Fan Xu Long Wang Yingbin Li Junfeng Shi Dorothee Staiger Feng Yu state Key Laboratory of Chemo/Biosensing and Chemometrics College of Biologyand Hunan Key Laboratory of Plant Functional Genomics and Developmental RegulationHunanUniversityChangsha 410082P.R.China State Key Laboratory of Hybrid Rice Hunan Agricultural Biotechnology Research InstituteHunan Academy of Agricultural SciencesChangsha 410125P.R.China RNA biology and Molecular Physiology Faculty of BiologyBielefeld University33615 BielefeldGermany

Changes in ambient temperature profoundly affect plant growth and ***,the molecu-larbasis of plant acclimation to temperature fluctuation is of great *** this study,we discovered that GLYCINE-RICH RNA-BINDING PROTEIN 7(GRP7)contributes to cold and heat tolerance in Arabidopsis *** found that exposure to a warm temperature rapidly induces GRP7 condensates in planta,which can be reversed by transfer to a lower *** biology and biochemical assays revealed that GRP7 undergoes liquid-liquid phase separation(LLPS)in vivo and in *** of GRP7 in the cyto-plasm contributes to the formation of stress granules that recruit RNA,along with the translation machinery component eukaryotic initiation factor 4E1(elF4E1)and the mRNA chaperones COLD SHOCK PROTEIN 1(CSP1)and CSP3,to inhibit ***,natural variations in GRP7 affecting the residue phos-phorylated by the receptorkinase FERONIA alter its capacity to undergo LLPS and correlate with the adap-tation of some Arabidopsis accessions to a widertemperature *** together,ourfindings illustrate the role of translational control mediated by GRP7 LLPS to confer plants with temperature resilience.

关键词： fluctuating temperature glycine-rich RNA-binding protein phase separation cold shock proteins translation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ubiquitination-mediated protein degradation and modification:an emerging theme in plant-microbe interactions

引用

Cell Research 2006年第5期16卷 413-426页

作者： Li-Rong Zeng~1 Miguel E Vega-Sánchez~1 Tong Zhu~2 Guo-Liang Wang~(1,3)~1Department of Plant Pathology and Plant Molecular biology and Biotechnology Program,The Ohio State University,Columbus,OH 43210,USA:~2Syngenta Biotechnology Inc.Research Triangle Park,NC 27709-2257.USA:~3Rice genomics Laboratory,Human Agricultural University,Changsha,Human 410128,China Department of Plant Pathology and Plant Molecular biology and Biotechnology Program The Ohio State University Columbus USA Syngenta Biotechnology Inc Research Triangle Park USA Rice genomics Laboratory Hunan Agricultural University Changsha China

Post-translational modification is central to protein stability and to the naodulation of protein *** types ofprotein modification,such as phosphorylation,methylation,acetylation,myristoylation,glycosylation,and ubiquitina-tion,have been *** them,ubiquitination distinguishes itself from others in that most of the ubiquitinatedproteins are targeted to the 26S proteasome for *** ubiquitin/26S proteasome system constitutes the majorprotein degradation pathway in the *** recent years,the importance of the ubiquitination machinery in the controlof numerous eukaryotic cellular functions has been increasingly *** number of E3 ubiquitin ligasesand their substrates,including a variety of essential cellular regulators have been *** in the past severalyears have revealed that the ubiquitination system is important for a broad range of plant developmental processes andresponses to abiotic and biotic *** review discusses recent advances in the functional analysis of ubiquitina-tion-associated proteins from plants and pathogens that play important roles in plant-microbe interactions.

关键词： Ubiquitination defense response plant-microbe interactions U-box protein Sp111

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：