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Human activities and elevational constraints restrict ranging patterns of snub-nosed monkeys in a mountainous refuge

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Integrative Zoology 2021年第2期16卷 202-213页

作者： Pengzhen HUANG Kun BIAN Zhipang HUANG Qi Li Derek WDUNN Gu FANG Jiahui LIU Mengyao WANG Xianfeng YANG Ruliang PAN Cunlao GAO Kaichuang SI Baoguo LI Xiaoguang QI Shaanxi Key Laboratory for Animal Conservation College of Life SciencesNorthwest UniversityXi’anChina Shaanxi Institute of Zoology Xi’anChina Zhouzhi National Nature Reserve Xi’anChina Center for Excellence in Animal Evolution and genetics Chinese Academy of SciencesKunmingChina

Both natural conditions and anthropogenic factors affect the survivability,distribution,and population density of *** understand the extent and how these factors drive species distributions,a detailed description of animal movement patterns in natural habitats is *** this study,we used satellite telemetry to monitor elevational ranges favored by endangered golden snub-nosed monkeys(Rhinopithecus roxellana),in the Qinling Mountains,central *** investigated the abundance and distribution of food resources through sampling vegetation quadrats at different elevations and sampled anthropogenic activities using field *** results indicated that although there was no significant variation in food resources between low-(<1500 m)and middle-elevations(1500–2200 m),monkeys were found most often in areas above 1500 m,where there was less anthropogenic development(*** and roads);however,monkeys rarely ranged above 2200 m and had limited food availability at this *** was limited human disturbance at this *** suggest that both human activity and ecological constraints(*** resources)have considerable effects on elevational use of *** in the Qinling *** study highlights the critical roles these factors can play in shaping the vertical distribution of high-altitude *** research provides useful insights for habitat-based conservation plans in which human disturbance management and habitat restoration should be prioritized.

关键词： elevational distribution food resources human activity Rhinopithecus roxellana satellite telemetry

Evolution of parthenogenetic reproduction in Caucasian rock lizards:A review

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Current Zoology 2023年第2期69卷 128-135页

作者： Marine Arakelyan Victor Spangenberg Varos Petrosyan Alexey Ryskov Oksana Kolomiets Eduard Galoyan Department of Zoology Yerevan State UniversityYerevanArmenia Vavilov Institute of General genetics MoscowRussia Severtsov Institute of Ecology and Evolution RAS MoscowRussia Institute of Gene Biology RASMoscowRussia Zoological Museum of Moscow State University MoscowRussia

Despite numerous works devoted to hybrid origin of parthenogenesis in reptiles,the causes of hybridization between different species,resulting in the origin of parthenogenetic forms,remain *** studies demonstrate that sexual species considered parental to parthenogenetic rock lizards(Darevskia spp.)avoid interspecific mating in the secondary overlap areas.A specific combination of environmental factors during last glaciation period was critical for ectotherms,which led to a change in their distribution and sex *** population structure(e.g.,male bias)and limited available distributional range favored the deviation of reproductive behavior when species switched to interspecific *** date,at least 7 diploid parthenogenetic species of rock lizards(Darevskia,Lacertidae)originated through interspecific hybridization in the *** cytogenetic specifics of meiosis,in particular the weak checkpoints of prophase l,may have allowed the formation of hybrid karyotypes in rock *** and polyploidization are 2 important evolutionary forces in the genus *** present,throughout backcrossing between parthenogenetic and parental species,the triploid and tetraploid hybrid individuals appear annually,but no triploid species found among Darevskia *** current stage of *** speciation by hybridization with the long-term stage of diploid parthenogenetic species,non-distorted meiosis,together with the high ecological plasticity of Caucasian rock lizards provide us with a new model for considering the pathways and persistence of the evolution of parthenogenesis in vertebrates.

关键词： Caucasus glacial period hybridization reptiles reticulate evolution unisexual

A Profile of Native Integration Sites Used by φC31 Integrase in the Bovine Genome

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Journal of genetics and Genomics 2012年第5期39卷 217-224页

作者： Lijuan Qu Qingwen Ma Zaiwei Zhou Haiyan Ma Ying Huang Shuzhen Huang Fanyi Zeng Yitao Zeng Shanghai Institute of Medical genetics Children＇s Hospital of Shanghai Shanghai Jiao Tong University School of Medicine 24/1400 West Beijing Road Shanghai 200040 China Key Lab of Medical Embryo Molecular Biology Ministry of Health Shanghai 200040 China Shanghai Key Lab of Embryo and Reproduction Engineering Shanghai Municipal Committee of Science and Technology Shanghai 200040 China Institute of Medical Science Shanghai Jiao Tong University School of Medicine 280 South Chongqing Road Shanghai 200025 China

The Streptomyces phage φC31 integrase can efficiently target attB-bearing transgenes to endogenous pseudo attP sites within mammalian genomes. To better understand the activity of φC31 integrase in the bovine genome, DNA sequences of 44 integration events were analyzed, and 32 pseudo attP sites were identified. The majority of these sites share a sequence motif that contains inverted repeats and has similarities to wild-type attP site. Genomic DNA flanking these sites typically contained repetitive sequence elements, such as short and long interspersed repetitive elements. These sequence features indicate that DNA sequence recognition plays an important role in guiding φC31-mediated site-specific integration. In addition, BF27 integration hotspot sites were identified in the bovine genome, which accounted for 13.6% of all isolated integration events and mapped to an intron of the deleted in liver cancer 1 （DLC1） gene. Also we found that the pseudo attP sites in the bovine genome had other features in common with those in the human genome. This study represents the first time that the sequence features of pseudo attP sites specific integrase system has great potential for applied modifications in the bovine genome were analyzed. We conclude that this site- of the bovine genome.

关键词： φC31 integrase Pseudo attP sites Sequence motif Repetitive elements Bovine genome

A community resource for exploring and utilizing genetic diversity in the USDA pea single plant plus collection

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Horticulture Research 2017年第1期4卷 293-300页

作者： William L.Holdsworth Elodie Gazave Peng Cheng James R.Myers Michael A.Gore Clarice J.Coyne Rebecca J.McGee Michael Mazourek Plant Breeding and genetics Section School of Integrative Plant ScienceCornell UniversityIthaca14853NYUSA Department of Crop and Soil Sciences Washington State UniversityPullman99164WAUSA Department of Horticulture Oregon State UniversityCorvallis97331ORUSA US Department of Agriculture Agricultural Research ServiceWestern Regional Plant Introduction StationPullman99164WAUSA US Department of Agriculture Agricultural Research ServiceGrain Legume Genetics and Physiology Research UnitPullman99164WAUSA Rupp Seeds WauseonOH 43567USA

Globally,pea(Pisum sativum L.)is an important temperate legume crop for food,feed and fodder,and many breeding programs develop cultivars adapted to these *** order to assist pea development efforts,we assembled the USDA Pea Single Plant Plus Collection(PSPPC),which contains 431 *** accessions with morphological,geographic and taxonomic *** collection was characterized genetically in order to maximize its value for trait mapping and genomics-assisted *** that end,we used genotyping-by-sequencing—a cost-effective method for de novo single-nucleotide polymorphism(SNP)marker discovery—to generate 66591 high-quality *** data facilitated the identification of accessions divergent from mainstream breeding germplasm that could serve as sources of novel,favorable *** particular,a group of accessions from Central Asia appear nearly as diverse as a sister species,***,and subspecies,*** *** genotypes can be paired with new and existing phenotype data for trait mapping;as proof-of-concept,we localized Mendel’s A gene controlling flower color to its known *** also used SNP data to define a smaller core collection of 108 accessions with similar levels of genetic diversity as the entire PSPPC,resulting in a smaller germplasm set for research screening and evaluation under limited *** together,the results presented in this study along with the release of a publicly available SNP data set comprise a valuable resource for supporting worldwide pea genetic improvement efforts.

关键词： breeding cultivar utilizing

Neurodegenerative changes initiated by presynaptic dysfunction

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Translational Neurodegeneration 2013年第1期2卷 108-112页

作者： Toru Yasuda Yasuto Nakata Chi-Jing Choong Hideki Mochizuki Department of Neurology Osaka University Graduate School of MedicineSuitaOsakaJapan Present address:Department of genetics National Center for Child Health and DevelopmentSetagaya-kuTokyoJapan

α-Synucleinopathies are a subgroup of neurodegenerative diseases including dementia with Lewy bodies(DLB)and Parkinson’s disease(PD).Pathologically,these disorders can be characterized by the presence of intraneuronal aggregates composed mainly of α-synuclein(αSyn),which are called Lewy bodies and Lewy *** report showed that more than 90% of αSyn aggregates are present in the form of very small deposits in presynaptic terminals of the affected neurons in ***,the mechanisms responsible for presynaptic accumulation of abnormal αSyn remain *** this article,we review recent findings on the involvement of presynaptic dysfunction in the initiation of neuronal dysfunctional *** review highlights that the presynaptic failure can be a potential trigger of the dying-back neuronal death in neurodegenerative diseases.

关键词： degenerative dysfunction involvement

Pharmacogenomics can improve antipsychotic treatment in schizophrenia

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Frontiers of Medicine 2013年第2期7卷 180-190页

作者： ingqing Xu Xi Wu Yuyu Xiong Qinghe Xing Lin He Shengying Qin Bio-X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education)Shanghai Jiao Tong UniversityShanghai 200030China Shanghai Genome Pilot Institutes for Genomics and Human Health Shanghai 200030China Institutes of Biomedical Sciences Fudan UniversityShanghai 200032China

Schizophrenia is a widespread mental disease with a prevalence of about 1%in the world population,and heritability of up to 80%.Drug therapy is an important approach to treating the ***,the curative effect of antipsychotic is far from satisfactory in terms of tolerability and side *** studies have indicated that about 30%of the patients exhibit little or no improvements associated with *** response of individual patients who are given the same dose of the same drug varies *** addition,antipsychotic drugs are often accompanied by adverse drug reactions(ADRs),which can cause considerable financial loss in addition to the obvious societal ***,it is strongly recommended that personalized medicine should be implemented both to improve drug efficacy and to minimize adverse events and *** is therefore a need for pharmacogenomic studies into the factors affecting response of schizophrenia patients to antipsychotic drugs to provide informed guidance for *** differences in drug response is due to a combination of many complex factors including ADEM(absorption,distribution,metabolism,excretion)process,transporting,binding with receptor and intracellular signal *** and pharmacogenomic studies have successfully identified genetic variants that contribute to this interindividual variability in antipsychotics *** addition,epigenetic factors such as methylation of DNA and regulation by miRNA have also been reported to play an important role in the complex interactions between the multiple genes and environmental factors which influence individual drug response phenotypes in *** this review,we will focus on the latest research on polymorphisms of candidate genes that code for drug metabolic enzymes(CYP2D6,CYP1A2,CYP3A4,etc.),drug transporters(mainly ABCB1)and neurotransmitter receptors(dopamine receptors and serotonin receptors,etc.).We also discuss the genome-wi

关键词： pharmacogenomics epigenetics schizophrenia antipsychotics

Presence of c.3956del C mutation in familial adenomatous polyposis patients from Brazil

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World Journal of Gastroenterology 2015年第31期21卷 9413-9419页

作者： Caroline Aquino Moreira-Nunes Diego di Felipe ávila Alcantara Sérgio Figueiredo Lima-Júnior Sandro Roberto de Araújo Cavalléro Juan Antonio Rey Giovanny Reboucas Pinto Paulo Pimentel de Assumpcao Rommel Rodriguez Burbano Biological Science Institute Federal University of Pará Nucleus of Research in Oncology Federal University of Pará Molecular Neuro-oncogenetics Laboratory Research Unit-Unidad de InvestigaciónHospital Universitario La Paz genetics and Molecular Biology Laboratory Federal University of Piaui

AIM: To characterize APC gene mutations and correlate them with patient phenotypes in individuals diagnosed with familial adenomatous polyposis(FAP) in northern Brazil. METHODS: A total of 15 individuals diagnosed with FAP from 5 different families from the north of Brazil were analyzed in this study. In addition to patients with histopathological diagnosis of FAP,family members who had not developed the disease were also tested in order to identify mutations and for possible genetic counseling. All analyzed patients or their guardians signed a consent form approved by the Research Ethics committee of the Jo?o de Barros Barreto University Hospital(Belem,Brazil). DNA extracted from the peripheral blood of a member of each of the affected families was subjected to direct sequencing. The proband of each family was sequenced to identify germline mutations using the Ion Torrent platform. To validate the detected mutations,Sanger sequencing was also performed. The samples from all patients were also tested for the identification of mutations by real-time quantitative polymerase chain reaction using the amplification refractory mutation system. RESULTS: Through interviews with relatives and a search of medical records,it was possible to construct genograms for three of the five families included in the study. All 15 patients from the five families with FAP exhibited mutations in the APC gene,and all mutations were detected in exon 15 of the APC gene. In addition to the patients with a histological diagnosis of FAP,family members without disease symptoms showed the mutation in the APC gene. In the present study,we detected two of the three most frequent germline mutations in the literature: the mutation at codon 1309 and the mutation at codon 1061. The presence of c.3956 del C mutation was found in all families from this study,and suggests that this mutation was introduced in the population of the State of Pará through ancestor immigration(i.e.,a de novo mutation that arose

关键词： Familial adenomatous polyposis APC Torrent sequencing Colorectal cancer

Systematic insertion mutagenesis of GntR family tran-scriptional regulator genes in Sinorhizobium meliloti

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Chinese Science Bulletin 2008年第2期53卷 215-226页

作者： YU AiYuan CHEN AiMin WANG Yi LUO Li ZHU JiaBi YU GuanQiao WANG YanZhang National Key Laboratory of Plant Molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Biological Sciences Department Lehman College The City University of New York New York 104681 USA

GntR-type transcriptional regulators regulate the most diverse biological processes in bacteria. Al- though GntR-type transcriptional regulators consist of the second largest family of transcriptional regulators in Sinorhizobium meliloti, little is known about their functions. In this study, we investigated 54 putative genes encoding GntR family of transcriptional regulators in S. meliloti Rm1021. Secondary structure analysis of the C-terminal domain of these putative transcriptional regulators indicated that thirty-seven were members of the FadR subfamily, ten of the HutC subfamily and five of the MocR subfamily. The remaining two did not fall into any specific subfamily category, and may form two new subfamilies. The 54 gntR genes were mutagenized by plasmid insertion mutagenesis to investigate their roles. We found that, of the 54 mutants, only the gtrA1 and gtrB1 mutants had slower growth rates and cell maximal yields on both rich medium and minimal medium, and lower cell motility on swarming plate than wild type Rm1021. All mutants, with the exception of gtrA1 and gtrB1, can establish effective symbioses with alfalfa. Plants inoculated with gtrA1 and gtrB1 mutants grew shorter than those in- oculated with wild type, and formed relatively smaller, round and light pink nodules, which were mainly located on lateral roots. And there was an abnormal increase in the number of nodules induced by both mutants. These results suggested that the gtrA1 and gtrB1 mutants were symbiotically deficient. Our work presents a global overview of GntR-like transcriptional regulators involved in symbiosis in S. meliloti, and provides new insight into the functions of GntR-like transcriptional regulators.

关键词：细菌诱变质粒插入调节剂

A new mutation (1062 del 16) of iduronate-2-sulfatase gene from a Chinese patient with Hunter syndrome

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第8期8卷 566-569页

作者： GUO Yi-bin PAN Jing-xin MENG Ya-xian Department of Medical genetics Sun Yat-sen Medical College Sun Yat-sen University Guangzhou 510080 China Department of Internal Medicine the Second Affiliated Hospital Fujian University of Medical Science Quanzhou 362000 China

Objective: To identify the mutations of iduronate-2-sulfatase (IDS) gene, to reveal its mutation features, and to establish a basis for genetic counseling and prenatal gene diagnosis of Hunter syndrome. Methods: Urine glycosaminoglycans (GAGs) assay, PCR and DNA sequencing were performed to detect mutation of IDS gene of the patient and his parents. Results: The result showed that the patient was: DS(++), HS(++), KS(-), CS(-), and that both of his parents were negative. A frame-shift deletion mutation (1062 del 16) was identified in exon 7 of the patient's IDS gene. His parents' genotypes were normal. Conclusion:The patient's mutation was not inherited by his parents but a novel one. The mutation probably altered the primary structure and tertiary structure of IDS enzyme protein remarkably and lowered the activity of IDS enzyme greatly. Therefore it is supposed to be the direct cause of the disorder.

关键词： Hunter syndrome Mucopolysaccharidosis typeⅡ (MPSⅡ) Glycosaminoglycan (GAG) Iduronate-2-sulfatase (IDS) Mutation Gene diagnosis DNA sequencing