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Consideration about investigation method of an industrial accident

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西安科技大学学报 2016年第5期36卷 737-740页

作者： YOMURA Tomoki SHI Gui-rong KARAMI Masume INOUE Shiichiro Ohara Memorial Institute for Science of Labour Tokyo Japan college of interhuman symbiotic studies Kanto Gakuin UniversityKanagawaJapan

The way to investigate the cause of an industrial accident is considered. It's important for the staff who investigate an accident to be the person who became independent from a production site. Moreover a special right has to be granted to the staff in the organization. The reason is because it's necessary that they have a different viewpoint from field overseer. The staff's viewpoint is a one related to the importance of the site preservation,necessity of an information feedback and the way to fill out an accident report. It was modeled in the last chapter about a relation of the various factors which have an influence on accident investigation.

关键词：工业事故事故分析安全事故事故处理

The nodule-specific transcriptional repressor Top Hub 4 regulates nodule structure and nitrogen fixation capacity in soybean

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Journal of Genetics and Genomics 2024年第1期51卷 96-99页

作者： Kui Ge Qi Lv Shengcai Chen Zhenhao Guo Yaqi Peng Yimian Chen Shiyong Sun Xuelu Wang college of Life Science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityZhengzhouHenan 450046China The Academy for Advanced Interdisciplinary studies Henan UniversityZhengzhouHenan 450046China Sanya Institute of Henan University SanyaHainan 572025China

Most leguminous plants establish symbiotic relationships with rhizobia to form root organs called nodules(Ferguson et al.,2010).Nodules are specialized organs containing bacterial symbionts,which can provide enormous amounts of fixed nitrogen to their plant hosts(Peoples et al.,2009).Soybean(Glycine max),an economically important grain and oil crop,forms symbiotic nitrogen-fixing nodules,which reduces the demand for chemical nitrogen fertilizers and promotes yield(Saito et al.,2014).Nodule development is spatiotemporally regulated by the action of a number of transcription factors(TFs).

关键词： specialized organs soybean

What factors control plant height?

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Journal of Integrative Agriculture 2024年第6期23卷 1803-1824页

作者： Li Miao Xiangyu Wang Chao Yu Chengyang Ye Yanyan Yan Huasen Wang college of Horticulture Qingdao Agricultural UniversityQingdao 266109China Key Laboratory for Quality and Safety Control of Subtropical Fruits and Vegetables College of Horticulture ScienceZhejiang Agriculture and Forestry UniversityHangzhou 311300China

Plant height(PH)is one of the most important components of the plant ideotype,and it affects plant biomass,yield,lodging resistance,and the ability to use mechanized *** many complex pathways controlling plant growth and development remain poorly understood,we are still unable to obtain the most ideal plants solely through breeding *** can be influenced by genotype,plant hormonal regulation,environmental conditions,and interactions with other ***,we comprehensively review the factors influencing PH,including the regulation of PH-related developmental processes,the genetics and QTLs contributing to PH,and the hormone-regulated molecular mechanisms for ***,the symbiotic influence of grafting on PH is discussed,focusing on the molecular regulation of gene expression and ***,we propose strategies for applying recent findings to breeding for better PH,highlight some knowledge gaps,and suggest potential directions for future studies.

关键词： dwarfism plant hormones interaction grafting QTLs phenotyping

Increased dependence on nitrogen-fixation of a native legume in competition with an invasive plant

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Plant Diversity 2024年第4期46卷 510-518页

作者： Meixu Han Haiyang Zhang Mingchao Liu Jinqi Tang Xiaocheng Guo Weizheng Ren Yong Zhao Qingpei Yang Binglin Guo Qinwen Han Yulong Feng Zhipei Feng Honghui Wu Xitian Yang Deliang Kong college of Forestry Henan Agricultural UniversityZhengzhou 450002China college of Life Science Hebei UniversityBaoding 071002China Liaoning Key Laboratory for Biological Invasions and Global Changes College of Bioscience and BiotechnologyShenyang Agricultural UniversityShenyangLiaoning 110866China college of Horticulture China Agricultural UniversityBeijing 100193China State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Arable Land Quality Monitoring and Evaluation Ministry of Agriculture and Rural AffairsInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China

Suppression of roots and/or their symbiotic microorganisms,such as mycorrhizal fungi and rhizobia,is an effective way for alien plants to outcompete native ***,little is known about how invasive and native plants interact with the quantity and activity of nutrient-acquisition *** a pot experiment was conducted with monoculture and mixed plantings of an invasive plant,Xanthium strumarium,and a common native legume,Glycine *** measured traits related to root and nodule quantity and activity and mycorrhizal *** to the monoculture,fine root quantity(biomass,surface area)and activity(root nitrogen(N)concentration,acid phosphatase activity)of *** decreased in mixed plantings;nodule quantity(biomass)decreased by 45%,while nodule activity in Nfixing via rhizobium increased by 106%;mycorrhizal colonization was *** of N fixation to leaf N content in *** increased in the mixed plantings,and this increase was attributed to a decrease in the rhizosphere soil N of *** in the mixed *** root quantity and activity,along with a higher mycorrhizal association was observed in *** in the mixed compared to ***,the invasive plant did not directly scavenge N from nodule-fixed N,but rather depleted the rhizosphere soil N of the legume,thereby stimulating the activity of N-fixation and increasing the dependence of the native legume on this N *** quantity-activity framework holds promise for future studies on how native legumes respond to alien plant invasions.

关键词： Mycorrhizal strategy Nitrogen depletion Plant invasion Root nutrient acquisition strategy symbiotic nitrogen fixation

Mycoendophytic diversity and their antimicrobial potential from two epiphytic orchids of the Western Ghats forests of India

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studies in Fungi 2020年第1期5卷 113-124页

作者： Nuthan BR Rakshith D Marulasiddaswamy KM Ramesha KP Chandra Mohana N Sampath Kumara KK Satish S Department of studies in Microbiology University of MysoreManasagangotriMysuru 570006KarnatakaIndia Department of Microbiology Yuvaraja’s CollegeUniversity of MysoreMysuru 570005KarnatakaIndia Department of studies in Biotechnology University of MysoreManasagangotriMysuru 570006KarnatakaIndia Government Pre-University college Davangere 577002KarnatakaIndia

The epiphytic plants belong to a group that harmlessly grows on other plants by utilizing the nutrition from the host plants with their unique adaptation features along with symbiotic associations with fungi or *** various biological activities exhibited by the mycoendophytes inhabiting medicinally-important epiphytic orchids serve as the primary source of novel drug leads,industrially-essential enzymes,and plant growth-promoting *** the present study,a total of 956 culturable mycoendophytes out of 1600 segments belonging to 17 genera were isolated from different tissue parts of Trias stocksii and Dendrobium *** Xylariaceae taxa were the predominant mycoendophytes present in both plants,followed by Pestalotiopsis sp.,Colletotrichum sp.,and Fusarium *** estimation of the Shannon-Wiener and Simpson diversity indices showed that the bulbs of *** have the highest species diversity index and the stems of *** the *** highest species richness was observed in the leaves of *** and the lowest in the leaves of ***,*** harbored more mycoendophytes along with the highest diversity indices compared to *** antimicrobial evaluation revealed that Xylaria *** a higher potential of producing anti-infectives and opens a new arena for industrial exploration.

关键词： Antimicrobial activity Dendrobium herbaceum Diversity indices Mycoendophytes Trias stocksii Xylaria sp

The role of rumen epithelial urea transport proteins in urea nitrogen salvage:A review

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Animal Nutrition 2022年第2期9卷 304-313页

作者： Chongliang Zhong Ruijun Long Gavin S.Stewart college of Ecology State Key Laboratory of Grassland Agro-ecosystemsLanzhou UniversityLanzhouChina School of Biology and Environmental Science University College DublinDublinIreland

The symbiotic relationship between the host and the rumen microbiome plays a crucial role in ruminant *** of the most important processes enabling this relationship is urea nitrogen salvaging(UNS).This process is important for both maintaining ruminant nitrogen balance and supporting production of their major energy supply,bacterially-derived short chain fatty acids(SCFA).The key step in UNS is the trans-epithelial movement of urea across the ruminal wall and this is a highly regulated *** the molecular level,the key transport route is via the facilitative urea transporter-B2,localized to ruminal papillae epithelial *** urea transport through aquaporins(AQP),such as AQP3,is now also viewed as ***-term regulation of these ruminal urea transport proteins appears to mainly involve dietary fermentable carbohydrates;whereas,transepithelial urea transport is finely regulated by local conditions,such as CO_(2) levels,pH and SCFA *** the key principles of ruminal urea transport physiology are now understood,there remains much that is unknown regarding the regulatory *** reason for this is the limited number of techniques currently used in many studies in the ***,future research in this area that combines a greater range of techniques could facilitate improvements to livestock efficiency,and potentially,reductions in the levels of waste nitrogen entering the environment.

关键词： Urea nitrogen salvaging Rumen Urea transporter Aquaporin UT-B AQP

A GmNINa-miR172c-NNC1 Regulatory Network Coordinates the Nodulation and Autoregulation of Nodulation Pathways in Soybean

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Molecular Plant 2019年第9期12卷 1211-1226页

作者： Lixiang Wang Zhengxi Sun Chao Su Yongliang Wang Qiqi Yan Jiahuan Chen Thomas Ott Xia Li State Key Laboratory of Agricultural Microbiology College of Plant Science and TechnologyHuazhong Agricultural UniversityNo.1 Shizishan RoadHongshan DistrictWuhanHubei 430070P.R.China college of Biological Science and Engineering Panzhihua UniversityNo.10 Airport RoadEastern DistrictPanzhihuaSichuanChina University of Freiburg.Faculty of Biology.University of Freiburg Schdnz丨estrasse 179104 Freiburg.Germany CIBSS-Centre for Integrative Biological Signalling studies University of Freiburg79104 FreiburgGermany

symbiotic root nodules are root lateral organs of plants in which nitrogen-fixing bacteria(rhizobia)convert atmospheric nitrogen to *** formation and number of nodules in legumes are precisely controlled by a rhizobia-induced signal cascade and host-controlled autoregulation of nodulation(AON).However,how these pathways are integrated and their underlying mechanisms are ***,we report that microRNA172c(miR172c)activates soybean(Glycine max)R hizobia-induced CLE1(GmRICI)and GmRIC2 by removing the transcriptional repression of these genes by Nodule Number Control 1(NNC1),leading to the activation of the AON ***1 interacts with GmNINa,the soybean ortholog of Lotus NODULE INCEPTION(NIN),and hampers its transcriptional activation o i G m RICI and ***,GmNINa acts as a transcriptional activator of ***,NNC1 can transcriptionally repress miR172c expression,adding a negative feedback loop into the NNC1 regulatory ***,GmNINa interacts with NNC1 and can relieve the NNC1-mediated repression of miR172c ***,the GmNINa-miR172c-NNC1 network is a master switch that coordinately regulates and optimizes NF and AON signaling,supporting the balance between nodulation and AON in soybean.

关键词： nodule number autoregulation of nodulation miR172c-NNC1 negative feedback loop nodule inception

GmNLP7a inhibits soybean nodulation by interacting with GmNIN1a

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作物学报:英文版 2023年第5期11卷 1401-1410页

作者： Xuesong Wu Yuping Xiong Jingjing Lu Mi Yang Hongtao Ji Xia Li Zhijuan Wang National Key Laboratory of Crop Genetic Improvement College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina

Nitrogen(N) is an essential macronutrient for plant growth and productivity. Leguminous plants establish symbiotic relationships with nitrogen-fixing rhizobial bacteria to use atmospheric dinitrogen gas to meet high N demand under low-N conditions. Nodule formation and N fixation are energy-consuming processes and are inhibited by nitrate present in the environment. Previous studies in model leguminous plants characterized NIN-LIKE PROTEIN(NLP) proteins that mediate nitrate control of root nodule symbiosis, but the mechanism by which nitrate regulates soybean root nodules via NLP remains unclear. In the soybean genome we found four homologs of AtNLP7, named GmNLP7a–GmNLP7d. We showed that the expression of GmNLP7s is responsive to nitrate but not to rhizobial infection and localized GmNLP7a to the nucleus. Downregulation of GmNLP7s increased nodule number, and overexpression of GmNLP7a(GmNLP7aOE) reduced nodule number regardless of nitrate availability, suggesting a negative role for GmNLP7s in nodulation. Nitrogenase activity in the GmNLP7aOE line was comparable to that of the wild type, indicating that GmNLP7a does not affect mature nodule activity. Overexpression of GmNLP7a downregulated the expression of GmNIN1a and GmENOD40-1. GmNLP7a interacted with GmNIN1a via the PB1domain. Our results reveal a new regulator of GmNLP7 in nodulation and a molecular mechanism by which nitrate affects nodule number in soybean.

关键词： Nodulation Nitrate GmNLP7 Soybean

Primers to block the amplification of symbiotic apostome ciliate 18S rRNA gene in a PCR-based copepod diet study

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Chinese Journal of Oceanology and Limnology 2014年第3期32卷 515-521页

作者：衣晓燕张寰刘光兴 college of Environmental Science and Engineering Ocean University of China Department of Marine Sciences Connecticut University Key Laboratory of Marine Environment and Ecology ( Ocean University of China ) Ministry of Education

Pelagic copepods play an important role in the marine food web. However, a full understanding of the ecological status of this zooplankton group depends on the careful study of their natural diets. In previous PCR-based copepod diet studies, we found many apostome ciliates that live symbiotically under the exoskeleton of the copepods, and their sequences were often over-represented in the 18S rRNA gene （18S rDNA） libraries. As a first step to address this issue, we designed three apostome ciliate 18S rDNA blocking primers, and tested their blocking efficiency against apostome ciliate 18S rDNA under various PCR conditions. Using a semi-quantitative PCR method, we optimized the conditions to efficiently amplify the 18S rDNA of the prey while simultaneously excluding the symbiotic apostome ciliates. This technique will facilitate PCR-based diet studies of copepods and other zooplankton in their natural environments.

关键词： copepod 18S rDNA apostome ciliate blocking primer PCR