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Mutation in the Agrobacterium his I gene enhances transient expression in pepper

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horticultural plant Journal 2024年第3期10卷 809-822页

作者： Dan Liu Shengnan Zhao Jubin Wang Xi Zhang Yingtian Deng Feng Li National key laboratory for Germplasm Innovation&Utilization of horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China

Agrobacterium-mediated plant transformation is widely used in plant genetic ***,its efficiency is limited by plant immunity against *** pepper(Capsicum annuum L.)is an important vegetable that is recalcitrant to Agrobacterium-mediated *** this work,Agrobacterium was found to induce a strong immune response in pepper,which might be the reason for T-DNA being difficult to express in *** Agrobacterium mutant screen was conducted and a point mutation in the hisI gene was identified due to a weak immune response and enhanced transient expression mediated by this Agrobacterium mutant in pepper *** genetic analysis revealed that histidine biosynthesis deficiency caused by mutations in many genes of this pathway led to reduced pepper cell death,presumably due to reduced bacterial ***,mutation analysis of threonine and tryptophan biosynthesis genes showed that the biosynthesis of different amino acids may play different roles in Agrobacterium growth and stimulating the pepper immune *** possible application of Agrobacterium amino acid biosynthesis mutations in plant biology was discussed.

关键词： Capsicum annuum L. Agrobacterium Transient transformation efficiency Mutation Amino acid Immune response

Identification of the lysine and histidine transporter family in Camellia sinensis and the characterizations in nitrogen utilization

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horticultural plant Journal 2024年第1期10卷 273-287页

作者： Wei Huang Danni Ma Fawad Zaman Xulei Hao Li Xia E Zhang Pu Wang Mingle Wang Fei Guo Yu Wang Dejiang Ni Hua Zhao National key laboratory for Germplasm Innovation&Utilization of horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China

In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino *** tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino ***,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea *** this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two *** modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs ***,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in *** expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and ***,the expression of all three genes could be induced by organic ***,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT ***,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.

关键词： Camellia sinensis Nitrogen Lysine and histidine transporter(LHT)family

Biochar alleviates apple replant disease by reducing the growth of Fusarium oxysporum and regulating microbial communities

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horticultural plant Journal 2024年第3期10卷 657-671页

作者： Yinghao Liu Can Wang Ran Chen Weitao Jiang Yun Li Chengmiao Yin Yanfang Wang Zhiquan Mao college of Chemistry and Material science Shandong Agricultural UniversityTaianShandong 271018China State key laboratory of Crop biology/college of horticultural science and Engineering Shandong Agricultural UniversityTaianShandong 271018China

Apple replant disease(ARD)negatively affects plant growth and reduces yields in replanted *** this study,biochar was applied to apple replant soil with Fusarium *** aim was to investigate whether biochar could promote plant growth and alleviate apple replant disease by reducing the growth of harmful soil microorganisms,changing soil microbial community structure and improving the soil *** experiment included five treatments:apple replant soil(CK),methyl bromide fumigation apple replant soil(FM),replant soil with biochar addition(2%),replant soil with *** spore solution(8×10^(7)spores·mL^(-1)),and replant soil with biochar and *** spore solution *** biomass,the activity of antioxidant enzymes in the leaves and roots,and soil environmental variables were *** community composition and community structure were analyzed using 16SrDNA and ITS2 gene *** significantly reduced the abundance of *** and increased soil microbial diversity and *** also increased the soil enzyme activities(urease,invertase,neutral phosphatase,and catalase),the biomass(plant height,fresh weight,dry weight)and the activity of antioxidant enzymes(superoxide dismutase,peroxidase,and catalase).The root indexes of apple seedlings was also increased in replant soil by *** sum,biochar promoted the growth of plants,improved the replant soil environment,and alleviated apple replant disease.

关键词： Biochar Fusarium oxysporum Apple replant disease Soil environment

Resistance index and browning mechanism of apple peel under high temperature stress

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horticultural plant Journal 2024年第2期10卷 305-317页

作者： Hui Wang Shuhui Zhang Zidun Wang Dongmei Li Leiyu Yan Yifeng Feng Xiaojie Liu Rongxin Chen Wenmin Fan Lulong Sun Zhengyang Zhao college of Horticulture Northwest A&F UniversityYanglingShaanxi 712100China college of horticultural science and Engineering Shandong Agricultural University State Key Laboratory of Crop BiologyTai'anShandong 271018China

Apples are one of the most important economic crops *** of global warming and an aggravation of environmental,abnormally high temperatures occur frequently in fruit-growing season and seriously affect normal fruit growth and reduce fruit quality and *** took five-year-old Ruixue’(Qinfu 1×Pink Lady;CNA20151469.1) fruits as test materials,and the ambient temperature during fruit development was *** results showed that during the fruit-growing season,especially during the rapid growth stage (July to August),the maximum daily temperature exceeded 30℃ and lasted for more than 40 *** determine the effects of high temperature stress on the apple fruit resistance,we treated expanding,veraison,and maturity-period fruits at different *** was found that the fruits of the expanding period showed strong resistance to high temperature stress,whereas during veraison and maturity,fruit resistance to high temperature stress decreased,and the fruit peel browning phenotype ***,the content of malonaldehyde (MDA),hydrogen peroxide (H_(2)O_(2)),and superoxide anion (O._(2)^(-)) in the peel gradually increased with increasing *** content of total phenols,flavanol,and flavonoids in the peel decreased substantially at 45℃.Moreover,it was found that polyphenol oxidase gene (MdPPO1) was most sensitive to high temperature stress in ***,transient and stable MdPPO1 overexpression significantly promoted peel *** transgenic materials were more sensitive to high temperatures,and browning was more severe compared to non-genetically modified organism (WT).Stable MdPPO1 knockout calli obtained via clustered regularly interspersed short palindromic repeats (CRISPR/Cas9) gene knockout technology reduced the browning phenotype,and the resultant fruits were not sensitive to the effects of high temperature ***,MdPPO1 expression may be a key factor of high temperature-related changes observed in the browni

关键词： Malus domestica Borkh High temperature stress Peel Browning MdPPO1

Improved genome annotation of Brassica oleracea highlights the importance of alternative splicing

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horticultural plant Journal 2024年第4期10卷 961-970页

作者： Yinqing Yang Lei Zhang Qi Tang Lingkui Zhang Xing Li Shumin Chen Kang Zhang Ying Li Xilin Hou Feng Cheng State key laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing 210095China State key laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural AffairsSino-Dutch Joint Laboratory of Horticultural GenomicsBeijing 100081China

Brassica oleracea has been developed into many important crops,including cabbage,kale,cauliflower,broccoli and so *** genome and gene annotation of cabbage(cultivar JZS),a representative morphotype of ***,has been widely used as a common reference in biological *** its genome assembly has been updated twice,the current gene annotation still lacks information on untranslated regions(UTRs)and alternative splicing(AS).Here,we constructed a high-quality gene annotation(JZSv3)using a full-length transcriptome acquired by nanopore sequencing,yielding a total of 59452 genes and 75684 ***,we re-analyzed the previously reported transcriptome data related to the development of different tissues and cold response using JZSv3 as a reference,and found that 3843 out of 11908 differentially expressed genes(DEGs)underwent AS during the development of different tissues and 309 out of 903 cold-related genes underwent AS in response to cold ***,we also identified many AS genes,including BolLHCB5 and BolHSP70,that displayed distinct expression patterns within variant transcripts of the same gene,highlighting the importance of JZSv3 as a pivotal reference for AS ***,JZSv3 provides a valuable resource for exploring gene function,especially for obtaining a deeper understanding of AS regulation mechanisms.

关键词： Brassica oleracea Oxford nanopore technologies Gene annotation Alternative splicing

Cytochrome P450 CitCYP97B modulates carotenoid accumulation diversity by hydroxylating b-cryptoxanthin in Citrus

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plant Communications 2024年第6期5卷 57-72页

作者： Yingzi Zhang Jiajing Jin Nan Wang Quan Sun Di Feng Shenchao Zhu Zexin Wang Shunxin Li Junli Ye Lijun Chai Zongzhou Xie Xiuxin Deng National key laboratory for Germplasm Innovation&Utilization of horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan laboratory WuhanHubei 430070China

Carotenoids in plant foods provide health benefits by functioning as provitamin *** ofthe vital provitamin A carotenoids,b-cryptoxanthin,is typically plentiful in citrus ***,little is known about the genetic basis of b-cryptoxanthin accumulation in ***,we performed a widely targeted metabolomic analysis of 65 major carotenoids and carotenoid derivatives to characterize carotenoid accumulation in Citrus and determine the taxonomic profile of *** used data from 81 newly sequenced representative accessions and 69 previously sequenced Citrus cultivars to reveal the genetic basis of b-cryptoxanthin accumulation through a genome-wide association *** identified a causal gene,CitCYP97B,which encodes a cytochrome P450 protein whose substrate and metabolic pathways in land plants were *** subsequently demonstrated that CitCYP97B functions as a novel monooxygenase that specifically hydroxylates the b-ring of b-cryptoxanthin in a heterologous expression *** planta experiments provided further evidence that CitCYP97B negatively regulates b-cryptoxanthin *** the sequenced Citrus accessions,we found that two critical structural cis-element variations contribute to increased expression of CitCYP97B,thereby altering b-cryptoxanthin accumulation in ***/introgression appear to have contributed to the prevalence of two cis-element variations in different Citrus types during citrus ***,these findings extend our understanding of the regulation and diversity of carotenoid metabolism in fruit crops and provide a genetic target for production of b-cryptoxanthin-biofortified products.

关键词： carotenoids b-cryptoxanthin cytochrome P450 CYP97B hydroxylation

A graph-based pan-genome of Brassica oleracea provides new insights into its domestication and morphotype diversification

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plant Communications 2024年第2期5卷 261-278页

作者： Ning Guo Shenyun Wang Tianyi Wang Mengmeng Duan Mei Zong Liming Miao Shuo Han Guixiang Wang Xin Liu Deshuang Zhang Chengzhi Jiao Hongwei Xu Liyang Chen Zhangjun Fei Jianbin Li Fan Liu State key laboratory of Vegetable Biobreeding National Engineering Research Center for VegetablesBeijing Key Laboratory of Vegetable Germplasm ImprovementKey Laboratory of Biology and Genetics Improvement of Horticultural Crops(North China)Beijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry ScienceBeijing 100097China Jiangsu key laboratory for horticultural Crop Genetic Improvement Vegetable Research InstituteJiangsu Academy of Agricultural ScienceNanjingJiangsuChina Smartgenomics Technology Institute Tianjin 301700China Boyce Thompson Institute IthacaNYUSA

The domestication of Brassica oleracea has resulted in diverse morphological types with distinct patterns of organ *** we report a graph-based pan-genome of *** constructed from high-quality genome assemblies of different *** pan-genome harbors over 200 structural variant hotspot regions enriched in auxin-andflowering-related *** genomic analyses revealed that early domestication of *** focused on leaf or stem *** resulting from agricultural practices and variety improvement were detected among different ***-sweep and pan-genome analyses identified an auxin-responsive small auxin up-regulated RNA gene and a CLAV-ATA3/ESR-RELATED family gene as crucial players in leaf–stem differentiation during the early stage of *** domestication and the BoKAN1 gene as instrumental in shaping the leafy heads of cabbage and Brussels *** pan-genome and functional analyses further revealed that variations in the BoFLC2 gene play key roles in the divergence of vernalization andflowering characteristics among different morphotypes,and variations in thefirst intron of BoFLC3 are involved infine-tuning theflowering process in *** study provides a comprehensive understanding of the pan-genome of *** and sheds light on the domestication and differential organ development of this globally important crop species.

关键词： Brassica oleracea graph-based pan-genome structural variants SV domestication morphotype diversification

High-quality assembled and annotated genomes of Nicotiana tabacum and Nicotiana benthamiana reveal chromosome evolution and changes in defense arsenals

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Molecular plant 2024年第3期17卷 423-437页

作者： Jubin Wang Qingling Zhang Jeffrey Tung Xi Zhang Dan Liu Yingtian Deng Zhendong Tian Huilan Chen Taotao Wang Weixiao Yin Bo Lij Zhibing Lai Savithramma P.Dinesh-Kumar Barbara Baker Feng Li National key laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China plant Gene Expression Center Department of Plant and Microbial BiologyUniversity of CaliforniaBerkeleyBerkeleyCA 94706USA college of plant science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China college of Life science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China Department of plant biology and The Genome Center College of Biological SciencesUniversity of CalforniaDavisDavisCA 95616USA The key laboratory of horticultural plant Genetic and Improvement of Jiangxi Province Institute of Biological ResourcesJiangxi Academy of SciencesNanchang 330299China institute of Vegetables and Flowers Jiangxi Academy of Agricultural SciencesNanchang 330200Chin Hubei Hongshan laboratory WuhanHubei 430070China

Nicotiana tabacum and Nicotiana benthamiana are widely used models in plant biology ***,genomic studies of these species have *** we report the chromosome-level reference genome assemblies for *** and *** with an estimated 99.5%and 99.8%completeness,*** transcription start and termination site sequencing methods were developed and used for accurate gene annotation in *** analyses revealed evidence for the parental origins and chromosome structural changes,leading to hybrid genome formation of each ***,theantiviral silencinggenesRDR1,RDR6,DCL2,DCL3,andAGO2were lost from one or both subgenomes in ***,while both homeologs were kept in ***,the *** genome encodes fewer immune receptors and signaling components than that of *** find-ings uncover possible reasons underlying the hypersusceptible nature of *** developed the user-friendly Nicomics(http:/***/Nicomics/)web server to facilitate better use of Nicotiana genomic resources as well as gene structure and expression analyses.

关键词： Nicotiana tabacum Nicotiana benthamiana genomics evolution disease resistance NLR

A novel histone methyltransferase gene Cg SDG40 positively regulates carotenoid biosynthesis during citrus fruit ripening

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Journal of Integrative Agriculture 2024年第8期23卷 2633-2648页

作者： Jialing Fu Qingjiang Wu Xia Wang Juan Sun Li Liao Li Li Qiang Xu National key laboratory for Germplasm Innovation&Utilization of horticultural Crops College of Horticulture&Forestry ScienceHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan laboratory Wuhan 430070China Institute of horticultural sciences Jiangxi Academy of Agricultural SciencesNanchang 330200China plant Breeding and Genetics Section School of Integrative Plant ScienceCornell UniversityNew York 14853USA Robert W.Holley Center for Agriculture and Health USDA-Agricultural Research ServiceCornell UniversityNew York 14853USA

The flesh color of pummelo(Citrus maxima)fruits is highly diverse and largely depends on the level of carotenoids,which are beneficial to human *** is vital to investigate the regulatory network of carotenoid biosynthesis to improve the carotenoid content in ***,the molecular mechanism underlying carotenoid accumulation in pummelo is not fully *** this study,we identified a novel histone methyltransferase gene,CgSDG40,involved in carotenoid regulation by analyzing the flesh transcriptome of typical white-fleshed pummelo,red-fleshed pummelo and extreme-colored F1 hybrids from a segregated pummelo *** of CgSDG40 corresponded to flesh color change and was highly coexpressed with ***,CgSDG40 and CgPSY1 are located physically adjacent to each other on the chromosome in opposite directions,sharing a partially overlapping promoter *** localization analysis indicated that CgSDG40 localizes to the *** of CgSDG40 significantly increased the total carotenoid content in citrus calli relative to that in wild *** addition,expression of CgPSY1 was significantly activated in overexpression lines relative to wild *** together,our findings reveal a novel histone methyltransferase regulator,CgSDG40,involved in the regulation of carotenoid biosynthesis in citrus and provide new strategies for molecular design breeding and genetic improvement of fruit color and nutritional quality.

关键词： pummelo SET domain protein epigenetic regulation PSY1 lycopene fruit quality fruit color