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Genes & Diseases 2024年第2期11卷 512-515页

作者： June-Ha Shin Hwa-Ryeon Kim Jae-Seok Roe Department of Biochemistry College of Life Science and BiotechnologyYonsei UniversitySeoul 120-749Republic of Korea

Tumor-protein 53(p53)is a transcription factor(TF)encoded by TP53(Trp53 in mice)and is a master regulator of tumor-suppressive programs'.Importantly,TP53 is the most frequently mutated gene in human cancer,and its loss of function contributes to tumor development,exemplified by variable p53 mutations detected in 50%-75%of pancreatic cancers.2 From these perceptions,a number of targets transcribed by p53 that confer tumor suppression have been ***,our understanding of how p53 prevents normal cells from initiating tumorigenesis is still *** complement this limitation,herein,we established a new approach to identify underlying effectors transcribed by p53 in normal physiology.

关键词： incomplete initiating herein

Matrine-loaded self-adhesive swelling microneedle for inflammation regulation to improve eczema treatment

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Marine life Science & Technology 2024年第3期6卷 535-546页

作者： Jiale Shen Jiarui Wang Meng Wu Yan Shi Minhyeock Lee Zhiguo Wang Ming Kong college of Marine life Sciences Ocean University of ChinaQingdao 266003China Department of Burn and Plastic Surgery the Affiliated Hospital of Qingdao UniversityQingdao UniversityQingdao 266021China Department of biotechnology College of Life Sciences and BiotechnologyKorea UniversitySeoul 02841Republic of Korea

Eczema is a common chronic dermatological *** treatments exhibit limited efficacy due to fast drug release resulting in short-term *** of a new treatment strategy that enables sustained drug release and longterm maintenance on the skin surface is necessary.A self-adhesive swelling microneedle patch(SDSMNs)was designed and constructed using a two-step casting *** adhesive substrate was prepared by blending gelatin and dopamine via oxidation of NaIO4,so it could adhere onto the skin surface as well as withstand repeated bending movement without *** swelling needles were fabricated using polyvinyl alcohol(PVA)and polyvinylpyrrolidone(PVP),which could swell by absorbing interstitial fluid and release the drug in a controlled *** also showed desirable antibacterial activities toward *** and *** adhesive microneedles loaded with matrine(MAT-SDSMNs),an antiinflammatory Chinese medicine,dramatically relieved eczema symptoms through IL-17 mediated inflammation *** use of MAT-SDSMNs significantly decreased the infiltration of inflammation cells and level of inflammatory cytokines,reduced the skin thickness,and increased collagen deposition fraction compared with conventional ointment or subcutaneous *** results suggested that MAT-SDSMNs can improve eczema treatment by regulating the local inflammatory microenvironment,providing a simple,self-administered sustainable strategy for eczema treatment.

关键词： Self-adhesive swelling microneedle Eczema Matrine Inflammation regulation

Acarbose glycosylation by AcbE for the production of acarstatins with enhancedα-amylase inhibitory activity

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Synthetic and Systems biotechnology 2024年第2期9卷 359-368页

作者： Xin Zhang Qungang Huang Ziyue Guo Feifei Cai Qianjin Kang Linquan Bai State Key Laboratory of Microbial Metabolism School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai200240China college of life Science Tarim UniversityAlar843300China

Acarbose is a potent glycosidase inhibitor widely used in the clinical treatment of type 2 diabetes mellitus(T2DM).Various acarbose analogs have been identified while exploring compounds with improved pharmacological *** this study,we found that AcbE from Actinoplanes ***50/110 catalyzes the production of acarbose analogs that exhibit significantly improved inhibitory activity towardsα-amylase than *** AcbE mainly catalyzed the formation of two new compounds,namely acarstatins A and B,using acarbose as *** high-resolution mass spectrometry,nuclear magnetic resonance,and glycosidase hydrolysis,we elucidated their chemical structures as O-α-d-maltosyl-(1→4)-acarbose and O-α-d-maltotriosyl-(1→4)-acarbose,*** A and B exhibited 1584-and 1478-fold greater inhibitory activity towards human salivaryα-amylase than ***,both acarstatins A and B exhibited complete resistance to microbiome-derived acarbose kinase 1-mediated phosphorylation and partial resistance to acarbose-preferred glucosidase-mediated ***,acarstatins A and B have great potential as candidate therapeutic agents for T2DM.

关键词： Acarbose analogs α-amylase inhibitory activity Actinoplanes sp. Transglycosylase

A chromatographic method for pursuing potential GPCR ligands with the capacity to characterize their intrinsic activities of regulating downstream signaling pathway

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Chinese Chemical Letters 2024年第10期35卷 425-429页

作者： Ting Li Xinxin Zheng Lejing Qu Yuanyuan Ou Sai Qiao Xue Zhao Yajun Zhang Xinfeng Zhao Qian Li Key Laboratory of Resource Biology and biotechnology in Western China Ministry of EducationCollege of Life SciencesNorthwest UniversityXi'an 710069China

GPCRs are dominant targets for approved drugs and the discovery of lead compound targeting them is still ***-based screening technique is a promising platform to uncover GPCR ***,the intrinsic activities of them are seldom simultaneously determined during the *** beta2-adrenoceptor(β2AR)as a probe,this work created a strategy for screening GPCR ligands with simultaneous characterization of their downstream G protein binding responses associated with *** strategy included(i)the design and expression of a protein miniature formed byβ2AR and G proteinα-subunit(Gαs)using circularly permuted HaloTag(cpHalo)as a flexible linker;(ii)immobilization of the miniature onto silica gel by a click dehalogenation reaction;(iii)systematic characterization of the immobilized miniature by fluorescent and chromatographic studies,and(iv)simulating of ligand-inducedβ2AR-Gαs signaling cascade by chromatographic assays using GTP as an *** immobilized miniature exhibited specificity toβ2AR and Gαs antibodies and *** specificity is stable at least within fifteen days with the variation less than 1%.The intrinsic activities ofβ2AR ligands were distinguished by the changes of GTP chromatographic behaviors on Gαs-cpHalo-β2AR *** strengthened the binding affinity and kinetics of GTP with Gαs,while antagonist did not give any effect on *** the intrinsic activity evaluation,we believe,it will improve the attributes of chromatographic methods for drug discovery efforts with minimizing false-positive results.

关键词： Intrinsic efficacy GPCR-G protein miniature Circularly permuted HaloTag High-performance receptor chromatography GTP binding Agonist/antagonist

Arabidopsis WRKY1 promotes monocarpic senescence by integrative regulation of flowering,leaf senescence,and nitrogen remobilization

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Molecular Plant 2024年第8期17卷 1289-1306页

作者： Wei Zhang Shufei Tang Xuying Li Yuanyuan Chen Jiajia Li Yuyang Wang Ruichao Bian Ying Jin Xiaoxian Zhu Kewei Zhang Zhejiang Provincial Key Laboratory of biotechnology on Specialty Economic Plants College of Life SciencesZhejiang Normal UniversityJinhuaZhejiang 321004P.R.China

Monocarpic senescence,characterized by whole-plant senescence following a single flowering phase,is widespread in seed plants,particularly in crops,determining seed harvest time and ***,how external and internal signals are systemically integrated into monocarpic senescence remains largely ***,we report that the Arabidopsis thaliana transcription factor WRKY1 plays essential roles in multiple key steps of monocarpic ***1 expression is induced by age,salicylic acid(SA),and nitrogen(N)*** and leaf senescence are accelerated in the WRKY1 overexpression lines but are delayed in the wrky1 *** combined DNA affinity purification sequencing and RNA sequencing analyses uncover the direct target genes of *** studies show that WRKY1 coordinately regulates three processes in monocarpic senescence:(1)suppressing FLOWERING LOCUS C gene expression to initiate flowering,(2)inducing SA biosynthesis genes to promote leaf senescence,and(3)activating the N assimilation and transport genes to trigger N *** summary,our study reveals how one stress-responsive transcription factor,WRKY1,integrates flowering,leaf senescence,and N remobilization processes into monocarpic senescence,providing important insights into plant lifetime regulation.

关键词： monocarpic senescence leaf senescence flowering time nitrogen remobilization salicylic acid WRKY transcription factor

Changes in microorganisms and metabolites during long-term fermentation of commercially manufactured anchovy sauce

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Food Science and Human Wellness 2024年

作者： Daehyeon Kim Seong-Eun Park Eun-Ju Kim Seung-Ho Seo Kwang-Moon Cho Sun Jae Kwon Hong-Seok Son Department of biotechnology， college of life Sciences and biotechnology， Korea University Sonlab Inc. 3. AccuGene Inc.

This study was performed to investigate the changes in microbial communities and metabolites during the long-term fermentation of commercially manufactured anchovy sauce. Samples of commercial anchovy sauce were collected from large-scale fermentation tanks with fermentation periods of up to 7.87 years. The complex bacterial community was simplified to two genera， Tetragenococcus and Halanaerobium， after approximately 0.55 years of fermentation. Although genera， such as Saccharomyces， Cladosporium， Candida， and Aspergillus， were relatively dominant， no clear pattern was identified in fungal community analysis. The longitudinal metabolite profile demonstrated that approximately half (55.8%) of the metabolites present in anchovy sauce were produced within a year of fermentation due to rapid fermentation. Despite the static microbial community， the contents of several metabolites including amino acids and biogenic amines changed continuously during the long-term fermentation of anchovy sauce. This study provides novel insights into the changes in microbiota and metabolites in fish sauce produced without any starter inoculation.

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Comprehensive analyses of the proteome and ubiquitome revealed mechanism of high temperature accelerating petal abscission in tree peony

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Horticultural Plant Journal 2024年第1期10卷 205-222页

作者： Chunying Liu Ziqi Liu Yanchao Yuan Yuxi Zhang Yifu Fang Junqiang Chen Shupeng Gai Key Laboratory of Plant biotechnology in Shandong Province College of Life SciencesQingdao Agricultural UniversityQingdaoShandong 266109China Shandong Academy of Forestry JinanShandong 250014China

Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry *** temperature accelerates flower senescence and abscission,but the associated mechanisms are poorly *** this study,the tandem mass tag(TMT)proteome and label-free quantitative ubiquitome from tree peony cut flowers treated with 20℃for 0 h(RT0),20℃or 28℃for 60 h(RT60 or HT60)were examined based on morphological observation,***,6970 proteins and 1545 lysine ubiquitinated(Kub)sites in 844 proteins were *** residues(such as glutamate and aspartate)neighboring the Kub sites were in preference,and 36.01%of the Kub sites were located on the protein *** differentially expressed proteins(DEPs)and Kub-DEPs in HT60 vs RT60 were mainly enriched in ribosomal protein,protein biosynthesis,secondary metabolites biosynthesis,flavonoid metabolism,carbohydrate catabolism,and auxin biosynthesis and signaling revealed by GO and KEGG analysis,accompanying the increase of endogenous abscisic acid(ABA)accumulation and decrease of endogenous indoleacetic acid(IAA)***,the expression patterns of six enzymes(SAMS,ACO,YUC,CHS,ANS and PFK)putatively with Kub modifications were analyzed by proteome and real-time quantitative *** cell-free degradation assays showed PsSAMS and PsACO proteins could be degraded via the 26 S proteasome system in tree peony ***,a working model was proposed for the acceleration of flower senescence and abscission by high *** summary,all results contributed to understanding the mechanism of flower senescence induced by high temperature and prolonging fluorescence in tree peony.

关键词： Tree peony Proteome Ubiquitome High temperature Flower senescence

Molecular mechanisms underlying the negative effects of transient heatwaves on crop fertility

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Plant Communications 2024年第9期5卷 101-115页

作者： Qian Yao Ping Li Xin Wang Shuhua Liao Pu Wang Shoubing Huang college of Agronomy and biotechnology China Agricultural UniversityBeijing 100193China

Transient heatwaves occurring more frequently as the climate warms,yet their impacts on crop yield are severely underestimated and even *** lasting only a few days or even hours during sensitive stages,such as microgametogenesis and flowering,can significantly reduce crop yield by disrupting plant *** advances in multi-omics and GWAS analysis have shed light on the specific organs(e.g.,pollen,lodicule,style),key metabolic pathways(sugar and reactive oxygen species metabolism,Ca2+homeostasis),and essential genes that are involved in crop responses to transient heatwaves during sensitive *** review therefore places particular emphasis on heat-sensitive stages,with pollen development,floret opening,pollination,and fertilization as the central narrative *** multifaceted effects of transient heatwaves and their molecular basis are systematically reviewed,with a focus on key structures such as the lodicule and tapetum.A number of heat-tolerance genes associated with these processes have been identified in major crops like maize and *** mechanisms and key heat-tolerance genes shared among different stages may facilitate the more precise improvement of heat-tolerant crops.

关键词： heat stress floret fertility pollen pollen tube growth seed set molecular mechanism

Combined Insights from Leachate Structure and Microstructure Characteristics for Eating Quality of Convenience Rice Processed by Super-Heated and Pressurized Steam Technologies

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Rice science 2024年第4期31卷 475-488,I0035,I0036页

作者： Mingyo HA Hyo-Young JEONG Ju Hun LEE Hyun-Jung CHUNG Division of Food and Nutrition Chonnam National UniversityGwangju 61186South Korea Department of biotechnology College of Life Sciences and BiotechnologyKorea UniversitySeoul 02841South Korea Division of Food Science&Culinary Arts Shinhan UniversityGyeonggi 11644South Korea

Convenience rice has become widely popular due to its easy availability for cooking. This study investigated the starch structure and composition of leachate and the microstructure of reheated convenience rice using novel processing technologies: super-heated steaming(SHS), auto-electric cooking(AEC), and pressurized-steam cooking(PSC). Additionally, the effect of two different target water contents(58% and 63%) was also evaluated. The PSC_63% sample had the highest total solids and amylopectin amount in the leachate. The amylopectin amount in the leachate differed significantly based on the targeted water content. Morphological characterization revealed that the swelling of starch and the coated layer on the surface of rice grains were most pronounced in the PSC_63% sample due to the pressure processing. The textural hardness of the AEC_58% sample was much higher than that of the other samples. The PSC_63% sample had the highest textural adhesiveness value, which can be attributed to the highest amylopectin amount in the leachate. Sensory characterization showed that the PSC_63% sample had the highest glossiness, whiteness, moistness, and overall acceptability. The principal component analysis score plots presented substantial differences in the leachate and textural and sensory characteristics of reheated convenience rice among the different processing technologies.

关键词： reheated convenience rice starch structure leachate multi-scale structure eating quality processing technology

Enhancement of porcine in vitro embryonic development through luteolin‑mediated activation of the Nrf2/Keap1 signaling pathway

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Journal of Animal Science and biotechnology 2024年第2期15卷 600-613页

作者： Se-Been Jeon Pil-Soo Jeong Min Ju Kim Hyo-Gu Kang Bong-Seok Song Sun-Uk Kim Seong-Keun Cho Bo-Woong Sim Futuristic Animal Resource&Research Center(FARRC) Korea Research Institute of Bioscience and Biotechnology(KRIBB)Cheongju 28116Republic of Korea Department of Animal Science College of Natural Resources&Life SciencePusan National UniversityMiryang 50463Republic of Korea Department of Animal Science and biotechnology College of Agriculture and Life ScienceChungnam National UniversityDaejeon 34134Republic of Korea Department of Functional Genomics University of Science and TechnologyDaejeon 34113Republic of Korea Department of Animal Science Life and Industry Convergence Research Institute(RICRI)College of Natural Resources&Life SciencePusan National UniversityMiryang 50463Republic of Korea

Background Oxidative stress,caused by an imbalance in the production and elimination of intracellular reactive oxygen species(ROS),has been recognized for its detrimental effects on mammalian embryonic ***(Lut)has been documented for its protective effects against oxidative stress in various ***,its specific role in embryonic development remains *** study aims to investigate the influence of Lut on porcine embryonic development and to elucidate the underlying *** After undergoing parthenogenetic activation(PA)or in vitro fertilization,embryos supplemented with 0.5μmol/L Lut displayed a significant enhancement in cleavage and blastocyst formation rates,with an increase in total cell numbers and a decrease in the apoptosis rate compared to the *** on D2 and D6 revealed that embryos with Lut supplementation had lower ROS levels and higher glutathione levels compared to the ***,Lut supplementation significantly augmented mitochondrial content and membrane ***,activation of the Nrf2/Keap1 signaling pathway was observed in embryos supplemented with Lut,leading to the upregulation of antioxidant-related gene transcription *** further validate the relationship between the Nrf2/Keap1 signaling pathway and effects of Lut in porcine embryonic development,we cultured PA embryos in a medium supplemented with brusatol,with or without the inclusion of *** positive effects of Lut on developmental competence were negated by brusatol *** Our findings indicate that Lut-mediated activation of the Nrf2/Keap1 signaling pathway contributes to the enhanced production of porcine embryos with high developmental competence,and offers insight into the mechanisms regulating early embryonic development.

关键词： Luteolin Mitochondrial function Nrf2/Keap1 signaling pathway Oxidative stress Porcine embryo development