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Coleoptile Purple Line Regulated by A-P Gene System Is a Valuable Marker Trait for Seed Purity Identification in Hybrid Rice

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Rice science 2022年第5期29卷 451-461,I0019-I0031页

作者： DU Shuanglin WANG Zhongwei CHEN Yun TAN Yao LI Xiang ZHU Wenping HE Guanghua LEI Kairong GUO Longbiao ZHANG Yi State key laboratory for Conservation and utilization of Bio-resources in Yunnan/Research Center for Perennial Rice Engineering and Technology in Yunnan/School of Agriculture Yunnan UniversityKunming 650091China Biotechnology Research Institute Chongqing Academy of Agricultural SciencesChongqing 401329China key laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute Academy of Agricultural SciencesSouthwest UniversityChongqing 400715China State key laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China

In plants,a large number of anthocyanin biosynthetic genes encoding enzymes and regulatory genes encoding transcription factors are required for anthocyanin *** purple lines are two purple lines on both sides of coleoptiles after seed ***,the molecular mechanism of coleoptile purple line is not clear in rice so *** this study,two major dominant genes,coleoptile purple line 1(OsCPL1,also known as OsC1)and coleoptile purple line 2(OsCPL2),were isolated via map-based cloning,and both of them were required for anthocyanin biosynthesis of coleoptile purple line in *** knockout and complementation experiments confirmed that OsC1 was required for purple color in most organs,such as coleoptile line,sheath,auricle,stigma and apiculus,whereas OsCPL2 was just required for coleoptile purple ***1 was predominantly expressed in coleoptiles,flag leaves,and green panicles,and highly expressed in young leaves,whereas OsCPL2 was predominantly expressed in coleoptiles,and extremely lowly expressed in the other tested ***-of-function of either OsC1 or OsCPL2 resulted in significant reduction of transcript levels of multiple anthocyanin biosynthesis genes in *** purple line was further used as a marker trait in hybrid *** identification in hybrid rice seeds via coleoptile purple line just needed a little water,soil and a small plate and could be completed within 5 *** marker and field identification analyses indicated that coleoptile purple line was reliable for the hybrid seed purity *** findings disclosed that coleoptile purple line in rice was regulated by two major dominant genes,OsC1 and OsCPL2,and can be used as a simple,rapid,accurate and economic marker trait for seed purity identification in hybrid rice.

关键词： OsC1 OsCPL2 coleoptile purple line anthocyanin seed purity identification marker trait

Catalytic conversion of biomass-derived compounds to various amino acids: status and perspectives

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Frontiers of Chemical Science and Engineering 2023年第7期17卷 817-829页

作者： Benjing Xu Jinhang Dai Ziting Du Fukun Li Huan Liu Xingxing Gu Xingmin Wang Ning Li Jun Zhao Engineering Research Center for Waste Oil Recovery Technology and Equipment of Ministry of Education Chongqing Technology and Business UniversityChongqing 400067China College of Environment and resources Chongqing Technology and Business UniversityChongqing 400067China key laboratory of Energy Resource utilization from Agriculture Residue Academy of Agricultural Planning and EngineeringMinistry of Agriculture and Rural AffairsBeijing 100125China Department of Biology Hong Kong Baptist UniversityHong KongChina

Amino acids are important nitrogen-containing chemicals that have a variety of ***,fermentation is the widely employed method to produce amino acids;however,the products are mostly limited to natural amino acids in the *** synthesis is an alternative approach for the synthesis of amino acids with different types and configurations,where the use of renewable biomass-based feedstocks is highly *** date,several lignocellulose and triacylglycerol-derived intermediates,typicallyα-keto acids andα-hydroxyl acids,have been transformed into amino acids via the amination reaction in the presence of additional nitrogen sources(i.e.,NH3·H2O).Making full use of inherent nitrogen in biomass(i.e.,chitin and protein)to produce amino acids avoids the use of extra nitrogen sources and meets the requirements of green chemistry,which is attracting increasing *** this review,we summarize different chemical-catalytic systems for the transformation of biomass to amino *** outlook on the challenges and opportunities for more effective production of amino acids from biomass by catalytic methods is provided.

关键词： biomass amino acids chitin nitrogen-containing compounds lignocellulose

Effect of heat input on microstructure and toughness of rare earthcontained C-Mn steel

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Journal of Iron and Steel Research International 2018年第10期25卷 1033-1042页

作者： Ming-ming Song Bo Song Sheng-hua Zhang Zhan-bing Yang Zheng-liang Xue Sheng-qiang Song Run-sheng Xu Zhi-bo Tong The State key laboratory of Refractories and Metallurgy Wuhan University of Science and Technology Wuhan 430081 Hubei China key laboratory for Ferrous Metallurgy and resources utilization of Ministry of Education Wuhan University of Science and Technology Wuhan 430081 Hubei China School of Metallurgical and Ecological Engineering University of Science and Technology Beijing Beijing 100083 China The key laboratory of Extraordinary Bond Engineering and Advanced Materials Technology Yangtze Normal University Chongqing 408100 China

Gleeble-1500D thermal simulator was used to simulate the thermal cycle of different welding processes in C-Mn steel. The toughness of steel matrix and heat-affected zone （HAZ） was investigated, and the microstructure and inclusion were characterized as well. The results showed that the welding process has a great influence on the microstructure in HAZ. When t8/5 （the cooling time from 800 to 500℃ ） value of the welding process is less than 111 s, the microstructure in HAZ is mainly bainite/Widmanstatten in spite of the addition of rare earth. However, when t8/5 value is more than 250 s, there are a lot of acicular ferrites in the steel containing rare earth, while the main microstructures are grain boundary ferrite and bainite/Widmanstatten in the steel without rare earth. The impact toughness of the HAZ at ambient temperature first decreases and then increases with the increase in t8/5 value. The impact toughness is the worst when t8/5 value is 111 s. Rare earth can improve the impact performance of HAZ at ambient temperature, especially when t8/5 value of the welding process is 445 s. After the rare earth treatment, the cooling rate of forming acicular ferrite is about 0.5-7.5 ℃/s. Acicular ferrite can form even during the welding process with larger t8/5 value up to 600 s in rare earth-treated steel.

关键词： Rare earth Heat-affected zone Microstructure Acicular ferrite Impact toughness

Medium-low temperature hydrothermal hydrolysis kinetic characteristics of concentrated wet microalgae biomass

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International Journal of Agricultural and Biological Engineering 2017年第1期10卷 154-162页

作者： Ding Xiaojian Huang Yun Liao Qiang Fu Qian Xia Ao Xiao Chao Zhu Xun Reungsang Alissara Liu Zhidan key laboratory of Low-grade Energy utilization Technologies and Systems(chongqing University) Ministry of EducationChongqing 400044China Institute of Engineering Thermophysics Chongqing UniversityChongqing 400044China Department of Biotechnology Faculty of TechnologyKhon Kaen UniversityKhon Kaen 40002Thailand laboratory of Environment-Enhancing Energy College of Water Resources and Civil EngineeringChina Agricultural UniversityBeijing 100083China

To improve microalgae biomass utilization efficiency during biofuel production process,medium-low temperature hydrothermal hydrolysis pretreatment was adopted in this *** pretreatment kinetic characteristics of concentrated wet microalgae Chlorella vulgaris biomass(50 g/L)under medium-low temperature hydrolysis(100°C-200°C)were experimentally *** hydrothermal hydrolysis kinetics describing the coupled effects of temperature,initial pressure and retention time then were proposed using response surface methodology(RSM).The maximum carbohydrate yield reached 327.3 mg/g dried biomass under initial pressure of 4 MPa at reaction temperature of 150°C for 120 *** maximum protein yield(321.5 mg/g dried biomass)was obtained under initial pressure of 4 MPa at reaction temperature of 200°C for 60 *** on the hydrothermal hydrolysis kinetic models,it was confirmed that temperature was the most important factor affecting both carbohydrate and protein release during hydrothermal hydrolysis *** initial pressure and retention time were significant to carbohydrate release,but not to protein ***,lipid was mainly distributed in microalgae residual and almost did not exist in supernatant(about 8.03 mg/g).And with assistance of mixed hexane and methanol(the ratio of hexane to methanol was 7:3),67.69%of microalgae lipid was extracted out from hydrothermal hydrolysed microalgae residual(123.3 mg/g dried biomass).

关键词： hydrothermal hydrolysis kinetic characteristics microalgae medium-low temperature biofuel response surface methodology

Role of stress,slenderness and foliation on large anisotropic deformations at deep underground excavations

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International Journal of Mining Science and Technology 2021年第4期31卷 577-590页

作者： Bo Hu Mostafa Sharifzadeh Xiating Feng Wenbin Guo Roo Talebi State key laboratory of Mountain Bridge and Tunnel Engineering School of Civil EngineeringChongqing Jiaotong UniversityChongqing 400074China Northern Star resources Ltd. KalgoorlieWA 6430Australia key laboratory of Ministry of Education on Safe Mining of Deep Metal Mines Northeastern UniversityShenyang 110819China Engineering Research Centre for the Safe Exploitation and Comprehensive utilization of Mineral resources at Universities of Inner Mongolia Hulunbuir UniversityHulunbuir 021008China Western Australian School of Mines(WASM) Curtin UniversityKalgoorlieWA 6430Australia

High stress concentrations around underground excavations can result in significant damage to deep hard-rock *** conditions can be the result of stopping activities,blasting,seismicity,or other mining *** anisotropic deformation and excavation closure,especially under high-stress conditions,are expected if the excavation is located in a foliated or thin-bedded rock *** this research,the behaviour of excavations under deep and high-stress conditions was investigated and *** main purpose was to enhance the existing knowledge of managing large anisotropic deformations and to help prepare suitable measures for handling such *** simulations using the distinct element method(DEM)and model calibration were performed to reproduce the anisotropic deformation of an ore drive based on the collected field ***,the roles of key factors(*** ratio,slenderness ratio,foliation orientation,and foliation considering excavation orientation)on the large deformation and damage depth of the excavations were *** study found that increasing both the stress ratio and slenderness ratio induced linear increases in wall closure and damage depth,whereas increasing the foliation angle first increases the deformation and damage depth and then reduces them both before and after 45.The wall closure and damage thickness decreased with increasing orientation *** deformation and damage levels were classified based on these factors.

关键词： Large anisotropic deformation Buckling Deep mining Slenderness ratio Intercept angle Foliation orientation

Landslide susceptibility mapping(LSM)based on different boosting and hyperparameter optimization algorithms:A case of Wanzhou District,China

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第8期16卷 3221-3232页

作者： Deliang Sun Jing Wang Haijia Wen YueKai Ding Changlin Mi key laboratory of GIS Application Research Chongqing Normal UniversityChongqing 401331China key laboratory of New Technology for Construction of Cities in Mountain Area Ministry of EducationChongqing UniversityChongqing 400045China National Joint Engineering Research Center of Geohazards Prevention in the Reservoir Areas Chongqing UniversityChongqing 400045China School of Civil Engineering Chongqing UniversityChongqing 400045China Natural resources Development Service Center of Linyi Linyi 276000China

Boosting algorithms have been widely utilized in the development of landslide susceptibility mapping(LSM)***,these algorithms possess distinct computational strategies and hyperparameters,making it challenging to propose an ideal LSM *** investigate the impact of different boosting algorithms and hyperparameter optimization algorithms on LSM,this study constructed a geospatial database comprising 12 conditioning factors,such as elevation,stratum,and annual average *** XGBoost(XGB),LightGBM(LGBM),and CatBoost(CB)algorithms were employed to construct the LSM ***,the Bayesian optimization(BO),particle swarm optimization(PSO),and Hyperband optimization(HO)algorithms were applied to optimizing the LSM *** boosting algorithms exhibited varying performances,with CB demonstrating the highest precision,followed by LGBM,and XGB showing poorer ***,the hyperparameter optimization algorithms displayed different performances,with HO outperforming PSO and BO showing poorer *** HO-CB model achieved the highest precision,boasting an accuracy of 0.764,an F1-score of 0.777,an area under the curve(AUC)value of 0.837 for the training set,and an AUC value of 0.863 for the test *** model was interpreted using SHapley Additive exPlanations(SHAP),revealing that slope,curvature,topographic wetness index(TWI),degree of relief,and elevation significantly influenced landslides in the study *** study offers a scientific reference for LSM and disaster prevention *** study examines the utilization of various boosting algorithms and hyperparameter optimization algorithms in Wanzhou *** proposes the HO-CB-SHAP framework as an effective approach to accurately forecast landslide disasters and interpret LSM ***,limitations exist concerning the generalizability of the model and the data processing,which require further exploration in subsequent studies.

关键词： Landslide susceptibility Hyperparameter optimization Boosting algorithms SHapley additive exPlanations(SHAP)

Ambient electrocatalytic synthesis of urea by co-reduction of NO_(3)^(-) and CO_(2) over graphene-supported In_(2)O_(3)

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Chinese Chemical Letters 2024年第3期35卷 538-543页

作者： Yini Mao Yong Jiang Hao Liu Yimin Jiang Ming Li Wei Su Rongxing He key laboratory of Luminescence Analysis and Molecular Sensing(Southwest University) Ministry of EducationCollege of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China key laboratory of Beibu Gulf Environment Change and resources utilization(Nanning Normal University) Ministry of EducationCollege of Chemistry and Life ScienceNanning Normal UniversityNanning 530000China

Urea plays a vital role in the sustainable development of mankind as it is one of the most important nitrogen *** synthesis of urea is accompanied by a high level of energy consumption while electrocatalytic methods suffer from low yields and poor *** work achieves efficient synthesis of urea by designing the graphene-In_(2)O_(3)electrocatalysts for the co-activated reduction of nitrate and carbon dioxide,where the formation rate of urea,Faraday efficiency(FE)and carbon selectivity at-0.35 V *** can reach 357.47μg mg^(-1)h^(-1),10.46%and~100%,***,the key intermediates in the C–N coupling reaction are demonstrated to be*NH_(2)and*CO_(2),which is of novelty compared to previous *** work may provide inspiration for subsequent studies on the reaction mechanism of the electrochemical synthesis of urea,as well as theoretical guidance for the sustainable synthesis of some other important chemical substances.

关键词： Graphene-supported In_(2)O_(3) Dispersion effect Efficient C-N coupling key intermediate Urea

Anthropogenic land use substantially increases riverine CO_(2) emissions

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Journal of Environmental Sciences 2022年第8期34卷 158-170页

作者： Shijie Gu Siyue Li Isaac R.Santos School of River and Ocean Engineering Chongqing Jiaotong UniversityChongqing 400074China key laboratory of Reservoir Aquatic Environment Chongqing Institute of Green and Intelligent TechnologyChinese Academy of SciencesChongqing 400714China chongqing School University of Chinese Academy of SciencesChongqing 400714China Research Center for Environmental Ecology and Engineering Institute of Changjiang Water Environment and Ecological SecuritySchool of Environmental Ecology and Biological EngineeringKey Laboratory for Green Chemical Process of Ministry of EducationKey Laboratory of Novel Reactor and Green Chemical Technology of Hubei ProvinceEngineering Research Center of Phosphorus Resources Development and Utilization of Ministry of EducationWuhan Institute of TechnologyWuhan 430205China Department of Marine Sciences University of GothenburgGothenburg 41296Sweden National Marine Science Centre Southern Cross UniversityCoffs HarbourNSW 2450Australia

Carbon dioxide(CO_(2)) emissions from inland waters to the atmosphere are a pivotal component of the global carbon budget. Anthropogenic land use can influence riverine CO_(2) emissions, but empirical data exploring cause-effect relationships remain limited. Here, we investigated CO_(2) partial pressures(pCO_(2)) and degassing in a monsoonal river(Yue River)within the Han River draining to the Yangtze in China. Almost 90% of river samples were supersaturated in CO_(2) with a mean ± standard deviation of 1474 ± 1614 μatm, leading to emissions of 557-971 mmol/m^(2)/day from river water to the atmosphere. Annual CO_(2) emissions were 1.6-2.8 times greater than the longitudinal exports of riverine dissolved inorganic and organic carbon. pCO_(2) was positively correlated to anthropogenic land use(urban and farmland), and negatively correlated to forest cover. p CO_(2) also had significant and positive relationships with total dissolved nitrogen and total dissolved phosphorus. Stepwise multiple regression models were developed to predict p CO_(2) . Farmland and urban land released nutrients and organic matter to the river system, driving riverine p CO_(2) enrichment due to enhanced respiration in these heterotrophic rivers. Overall, we show the crucial role of land use driving riverine pCO_(2) , which should be considered in future large-scale estimates of CO_(2) emissions from streams. Land use change can thus modify the carbon balance of urban-river systems by enhancing river emissions, and reforestation helps carbon neutral in rivers.

关键词： River carbon Inland waters Greenhouse gases Climate change Water quality Headwater streams

Global gene expression profiling of perirenal brown adipose tissue whitening in goat kids reveals novel genes linked to adipose remodeling

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1008-1026页

作者： Le Zhao Haili Yang Minhao Li Min Xiao Xingchun Li Lei Cheng Wenqiang Cheng Meixi Chen Yongju Zhao College of Animal Science and Technology Southwest UniversityChongqing Key Laboratory of Herbivore ScienceChongqing 400715China

Background Brown adipose tissue(BAT)is known to be capable of non-shivering thermogenesis under cold stimulation,which is related to the mortality of *** the previous study,we observed that goat BAT is mainly located around the kidney at birth,and changes to white adipose tissue(WAT)in the perirenal adipose tissue of goats within one month after ***,the regulatory factors underlying this change is remain *** this study,we systematically studied the perirenal adipose tissue of goat kids in histological,cytological,and accompanying molecular level changes from 0 to 28 d after *** Our study found a higher mortality rate in winter-born goat kids,with goat birthing data *** we used thermal imaging revealing high temperature in goat hips at postnatal 0 d and gradually decrease during 28 *** is consistent with the region of perirenal BAT deposition and highlights its critical role in energy expenditure and body temperature regulation in goat ***,we found a series of changes of BAT during the first 28 d after birth,such as whitening,larger lipid droplets,decreased mitochondrial numbers,and down-regulation of key thermogenesis-related genes(UCP1,DIO2,UCP2,CIDEA,PPARGC1a,C/EBPb,and C/EBPa).Then,we used RNA-seq found specific marker genes for goat adipose tissue and identified 12 new marker genes for BAT and 10 new marker genes for WAT of ***,12 candidate genes were found to potentially regulate goat BAT *** mechanism of the change of this biological phenomenon does not involve a large-scale death of brown adipocytes and subsequent proliferation of white *** apoptosis may play a limited role,it is largely not critical in this transition *** We concluded that perirenal BAT plays a crucial role in thermoregulation in newborn goat kids,with notable species differences in the expression of adipose tissue marker genes,and we highlighted some potential marker genes

关键词： Adipose remodeling Brown adipose tissue Goat kids key thermogenesis-related genes Whitening