Global gene expression profiling of perirenal brown adipose tissue whitening in goat kids reveals novel genes linked to adipose remodeling

作     者：Le Zhao Haili Yang Minhao Li Min Xiao Xingchun Li Lei Cheng Wenqiang Cheng Meixi Chen Yongju Zhao Le Zhao;Haili Yang;Minhao Li;Min Xiao;Xingchun Li;Lei Cheng;Wenqiang Cheng;Meixi Chen;Yongju Zhao

作者机构：College of Animal Science and TechnologySouthwest UniversityChongqing Key Laboratory of Herbivore ScienceChongqing 400715China 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2024年第15卷第3期

页      面：1008-1026页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：This work was financially supported by The National Key Research and Development Program of China(No.2022YFD1300202) The National Natural Science Foundation of China(No.32372834) Chongqing Modern Agricultural Industry Technology System(CQMAITS202313) the Collection,Utilization and Innovation of Germplasm Resources by Research Institutes and Enterprises of Chongqing,China(cqnyncw-kqlhtxm) the Chongqing Postgraduate Research Innovation Project(CYB22141) 

主　　题：Adipose remodeling Brown adipose tissue Goat kids Key thermogenesis-related genes Whitening 

摘      要：Background Brown adipose tissue(BAT)is known to be capable of non-shivering thermogenesis under cold stimulation,which is related to the mortality of *** the previous study,we observed that goat BAT is mainly located around the kidney at birth,and changes to white adipose tissue(WAT)in the perirenal adipose tissue of goats within one month after ***,the regulatory factors underlying this change is remain *** this study,we systematically studied the perirenal adipose tissue of goat kids in histological,cytological,and accompanying molecular level changes from 0 to 28 d after *** Our study found a higher mortality rate in winter-born goat kids,with goat birthing data *** we used thermal imaging revealing high temperature in goat hips at postnatal 0 d and gradually decrease during 28 *** is consistent with the region of perirenal BAT deposition and highlights its critical role in energy expenditure and body temperature regulation in goat ***,we found a series of changes of BAT during the first 28 d after birth,such as whitening,larger lipid droplets,decreased mitochondrial numbers,and down-regulation of key thermogenesis-related genes(UCP1,DIO2,UCP2,CIDEA,PPARGC1a,C/EBPb,and C/EBPa).Then,we used RNA-seq found specific marker genes for goat adipose tissue and identified 12 new marker genes for BAT and 10 new marker genes for WAT of ***,12 candidate genes were found to potentially regulate goat BAT *** mechanism of the change of this biological phenomenon does not involve a large-scale death of brown adipocytes and subsequent proliferation of white *** apoptosis may play a limited role,it is largely not critical in this transition *** We concluded that perirenal BAT plays a crucial role in thermoregulation in newborn goat kids,with notable species differences in the expression of adipose tissue marker genes,and we highlighted some potential marker genes