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Effect of mini-cutting size on adventitious rooting and morphophysiological quality of Ilex paraguariensis plantlets

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Journal of Forestry Research 2021年第2期32卷 815-822页

作者： Nathalia Pimentel denise Gazzana Janaina de Fatima Spanevello Kelen Haygert Lencina Dilson Antônio Bisognin Programa de Pós-Graduação em Engenharia Florestal Centro de Ciências Rurais da Universidade Federal de Santa MariaAvenida Roraima1000Santa MariaRS 97105-900Brazil Curso de Graduação em Agronomia Centro de Ciências Rurais da Universidade Federal de Santa MariaAvenida Roraima1000Santa MariaRS 97105-900Brazil departamento de Fitotecnia Centro de Ciências RuraisUniversidade Federal de Santa MariaAv.Roraima1000Santa MariaRS 97105-900Brazil

As the energizing and therapeutic effects of infusions of mate(Ilex paraguariensis ***.-Hil)leaves are increasingly recognized,rising global consumption is also increasing the demand for plantlets with high genetic and morphological ***-cuttings are an alternative for clonal plantlets;however,more details are needed on factors that affect the success of this ***,toward maximizing production,we evaluated adventitious rooting competence of mini-cuttings of different sizes and the morphophysiological quality of the resultant ***(one bud;length up to 2.5 cm),medium(multiple buds;length 2.6–5.0 cm),and large(multiple buds;length 5.1–10.0 cm)mini-cuttings were planted in a mixture of commercial substrate,vermiculite,and coarse sand(1:1:1 v:v:v),grown in a humidity chamber,then evaluated after 45,60,75,and 90 days for survival,percentage of callus and shoot formation,number and length of shoots,percentage of rooted cuttings,and number and length of *** rooted mini-cuttings were then cultivated in commercial substrate and subsurface soil(2:1 v:v)and evaluated after 30,60,90 and 120 days of cultivation for morphophysiology of the plantlets,percentage of survival,number of leaves,shoot height,stem diameter,and ratio of shoot height to stem *** 120 days,the plantlets were evaluated for the ratio of shoot and root dry mass,total length,surface area,total volume,and mean diameter of roots and Dickson quality *** rooting of the mini-cuttings and the morphophysiological quality of the plantlets were not influenced by the size of the vegetative ***,single-bud mini-cuttings have similar adventitious rooting competence,and result in plantlets with adequate morphophysiological quality,based upon leaf development,shoot height,stem diameter and length,and surface area,volume and diameter of ***-bud mini-cuttings up to 2.5 cm long can thus be used to maximize production of mate plantlets by mini-c

关键词： Aquifoliaceae Clonal forestry Mini-cutting technique Plantlet production Vegetative propagation

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Contribution of glial cells to the neuroprotective effects triggered by repetitive magnetic stimulation:a systematic review

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Neural Regeneration Research 2024年第1期19卷 116-123页

作者： Susana A.Ferreira Nuno Pinto Inês Serrenho Maria Vaz Pato Graça Baltazar centro de Investigação em ciências da Saúde(ciCS-UBI) Universidade da Beira InteriorCovilhãPortugal Faculdade de ciências da Saúde Universidade da Beira InteriorCovilhãPortugal GRUBI-Systematic Reviews Group University of Beira InteriorCovilhãPortugal

Repetitive transcranial magnetic stimulation has been increasingly studied in different neurological diseases,and although most studies focus on its effects on neuronal cells,the contribution of nonneuronal cells to the improvement trigge red by repetitive transcranial magnetic stimulation in these diseases has been increasingly *** systematically review the effects of repetitive magnetic stimulation on non-neuronal cells two online *** of Science and PubMed were searched fo r the effects of high-frequency-repetitive transcranial magnetic stimulation,low-frequencyrepetitive transcranial magnetic stimulation,intermittent theta-bu rst stimulation,continuous thetaburst stimulation,or repetitive magnetic stimulation on non-neuronal cells in models of disease and in unlesioned animals or cells.A total of 52 studies were *** protocol more frequently used was high-frequency-repetitive magnetic stimulation,and in models of disease,most studies report that high-frequency-repetitive magnetic stimulation led to a decrease in astrocyte and mic roglial reactivity,a decrease in the release of pro-inflammatory cyto kines,and an increase of oligodendrocyte *** trend towards decreased microglial and astrocyte reactivity as well as increased oligodendrocyte proliferation occurred with intermittent theta-burst stimulation and continuous theta-burst *** papers analyzed the low-frequency-repetitive transcranial magnetic stimulation protocol,and the parameters evaluated were restricted to the study of astrocyte reactivity and release of pro-inflammatory cytokines,repo rting the absence of effects on these paramete *** what concerns the use of magnetic stimulation in unlesioned animals or cells,most articles on all four types of stimulation reported a lack of *** is also important to point out that the studies were developed mostly in male rodents,not evaluating possible diffe rential effects of repetitive transcranial magneti

关键词： astrocyte glia high-frequency repetitive magnetic stimulation inflammation low-frequency repetitive magnetic stimulation microglia neurologic disorders oligodendrocyte repetitive magnetic stimulation theta-burst stimulation

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Flow regimes in wet conical spouted beds using glass bead mixtures

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Particuology 2008年第2期6卷 72-80页

作者： Marcelo S.Bacelos Jos Teixeira Freire Universidade Federal de Sāo Carlos Centro de Ciências Exatas e de TecnologiaPrograma de Pós-Graduacāo em Engenharia QuímicaCentro de Secagem de PastasSuspensōes e SementesRod.Washington LuizKm 23513565-905CP 676Sāo CarlosSPBrazil

Using a high-viscosity Newtonian fluid, glycerol, an experimental investigation was carried out to evaluate the stable spouting regime in conical spouted beds using four particle mixtures： a reference （monoparticles）, a binary mixture, two ternary mixtures with flat and Gaussian distributions respectively. The mixtures were selected for particle diameters （dp） ranging from 1.09 to 4.98 mm and particle diameter ratios （dpL/dps） ranging from 1.98 to 4.0. Experimental data show that pressure fluctuation signals of the bed, as indicated by changes in their standard deviations, provide suitable information to identify the range of operational conditions for stable spouting. However, the analysis of skewness of curves of pressure fluctuation as a function of air velocity appears not sufficient to identify a particular flow regime. For glycerol in the spouting regime, the standard deviation is noted to increase with increasing glycerol concentration due to the growth of interparticle forces. The implications of these research findings on the drying of suspensions in conical spouted beds using glass bead mixtures are also discussed.

关键词： Conical spouted bed Binary and ternary mixtures Processing of pastes Spouting regimes Spouting stability Pressure fluctuation

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The spindle assembly checkpoint:perspectives in tumorigenesis and cancer therapy

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Frontiers in Biology 2011年第2期6卷 147-155页

作者： Joana BARBoSA Ana Vanessa NASciMENTo Juliana FARIA Patrícia SILVA Hassan BoUSBAA centro de Investigação em ciências da Saúde(ciCS) Instituto Superior de Ciências da Saúde-NorteCESPURua Central de Gandra13174585-116 Gandra PRDPortugal

Loss or gain of chromosomes,a condition known as aneuploidy,is a common feature of tumor cells and has therefore been proposed as the driving force for *** chromosomal instability can arise during mitosis as a result of mis-segregation of the duplicated sister chromatids to the two daughter *** normal cells,missegregation is usually prevented by the spindle assembly checkpoint(SAC),a sophisticated surveillance mechanism that inhibits mitotic exit until all chromosomes have successfully achieved bipolar attachment to spindle *** abrogation of SAC activity is lethal to normal as well as to tumor cells,as a consequence of massive chromosome ***,many human aneuploid tumor cells exhibit a weakened SAC activity that allows them to tolerate gains or losses of a small number of chromosomes;and interfering with this SAC residual activity may constitute a suitable strategy to kill cancer *** review focuses on the potential link between SAC and tumorigenesis,and the therapeutic strategy to target the SAC for cancer treatment.

关键词： spindle assembly checkpoint mitosis chromosome instability tumor cancer therapy

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Epigenetic marks are modulated by gender and time of the day in the hippocampi of adolescent rats:a preliminary study

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Neural Regeneration Research 2018年第12期13卷 2160-2163页

作者： Viviane Rostirola Elsner Laura Reck Cechinel Louisiana Carolina Ferreira de Meireles Karine Bertoldi Ionara Rodrigues Siqueira Programa de Pós-Graduacao em ciências Biológicas:Fisiologia Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do Sul Programa de Pós-Graduacao em Biociências e Reabilitacao Centro Universitário Metodista-IPA Curso de Fisioterapia Centro Universitário Metodista-IPA

Although the involvement of gender in epigenetic machinery in peripheral tissues during the neonatal period has been suggested, the gender-related epigenetic profile of brain areas during the adolescent period is rarely exploited. Furthermore, the influence of time of day on hippocampal acetylation marks has been demonstrated in young adult and aged rats; however, there are no studies reporting epigenetic changes in the adolescent period. Therefore, this study aimed to investigate the effects of gender on hippocampal DNA methyltransferase 1 content and histone deacetylase（HDAC） activity of adolescent rats at different time points, specifically early morning and afternoon. Both epigenetic markers increased significantly in the hippocampi of female rats compared to the male group, an indicator of reduced transcriptional activity. In addition, HDAC activity during the early morning was higher compared to afternoon groups in both male and female rats, while DNA methyltransferase 1 content was not altered by the time of day. our findings demonstrate that hippocampal DNA methylation and histone acetylation status can be influenced by gender during the adolescent period, while the time of the day impacts HDAC activity.

关键词： adolescent rats DNA methyltransferase 1 histone deacetylase hippocampus time of the day gender epigenetic marks

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Soil and climate equally contribute to changes in the species compositions of Brazilian dry forests across 300 km

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Journal of Plant Ecology 2020年第2期13卷 171-176页

作者： Daniel M.Arruda Luiz F.S.Magnago Ricardo R.C.Solar Reinaldo Duque-Brasil Priscyla M.S.Rodrigues Rubens M.Santos Carlos E.G.R.Schaefer Instituto de Geociências Universidade Federal de Minas GeraisBelo HorizonteMinas GeraisBrazil centro de formação em ciências e Tecnologias agroflorestais Universidade Federal do Sul da BahiaItabunaBahiaBrazil Instituto de ciências Biológicas Universidade Federal de Minas GeraisBelo HorizonteMinas GeraisBrazil Instituto de ciências da Vida Universidade Federal de Juiz de ForaGovernador ValadaresMinas GeraisBrazil Colegiado de Ecologia Universidade Federal do Vale do São FranciscoSenhor do BonfimBahiaBrazil departamento de ciências Florestais Universidade Federal de LavrasLavrasMinas GeraisBrazil departamento de Solos Universidade Federal de ViçosaViçosaMinas GeraisBrazil

Aims Understanding the factors that control biodiversity distributions at different spatial scales has been a key challenge for conservation *** biodiversity,reflected in differences in species compositions among sites(beta diversity),can be derived from species replacement(turnover)and is driven by multiple ***,we sought to tackle this issue through two questions related to threatened Brazilian seasonally dry forests:(i)what is the contribution of species turnover to beta diversity?and(ii)which factors drive variations in species compositions among forest patches?Methods We sampled tree species and environmental variables(soils and climate)in 17 dry forest patches spaced almost 300 km *** used the beta diversity partitioning framework to determine the contribution of *** used redundancy analysis,with properly controlled spatial structure,to assess the contributions of the environmental and spatial factors to the variations of the species *** Findings Beta diversity among the patches was mainly represented by the turnover component(98.2%),with Simpson dissimilarity superior to other regions of the country(means of 0.89 and 0.71 in multiple site and pairwise measures,respectively).The environmental factors measured explained more than space,representing 30.3%of the variation of the species composition,of which 28.1%was nonspatially *** suggest that 300 km represents a threshold at which edaphic and climatic predictors have similar effects in determining community turnover(14.9%and 12.6%,respectively,without spatial structure).Thus,conservation strategies should be considered across landscapes to effectively protect tropical forest diversity,as even considering the different climatic aspects covered by the scale,landscaped edaphic varieties are important drivers of species turnover.

关键词： beta diversity environmental filter habitat fragmentation landscape structure phytogeography

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Molecular approaches for spinal cord injury treatment

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Neural Regeneration Research 2023年第1期18卷 23-30页

作者： Fernanda Martins de Almeida Suelen Adriani Marques Anne Caroline Rodrigues dos Santos Caio Andrade Prins Fellipe Soares dos Santos Cardoso Luiza dos Santos Heringer Henrique Rocha Mendonça Ana Maria Blanco Martinez Laboratório de Neurodegeneração e Reparo Programa de Pós-Graduação em Medicina:Anatomia PatológicaDepartamento de PatologiaFaculdade de MedicinaHospital Universitário Clementino Fraga FilhoCentro de Ciências da SaúdeUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil Instituto de ciências Biomédicas Centro de Ciências da SaúdeUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil Instituto de Biologia Departamento de NeurobiologiaLaboratório de Regeneração Neural e FunçãoUFFRio de JaneiroBrazil Laboratório Integrado de Morfologia Instituto de Biodiversidade e Sustentabilidade NUPEMPrograma de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFisCentro Multidisciplinar UFRJ-MacaéUniversidade Federal do Rio de JaneiroMacaéBrazil

Injuries to the spinal cord result in permanent disabilities that limit daily life *** main reasons for these poor outcomes are the limited regenerative capacity of central neurons and the inhibitory milieu that is established upon traumatic *** decades of research,there is still no efficient treatment for spinal cord *** strategies are tested in preclinical studies that focus on ameliorating the functional outcomes after spinal cord *** these,molecular compounds are currently being used for neurological recovery,with promising *** molecules target the axon collapsed growth cone,the inhibitory microenvironment,the survival of neurons and glial cells,and the re-establishment of lost *** this review we focused on molecules that are being used,either in preclinical or clinical studies,to treat spinal cord injuries,such as drugs,growth and neurotrophic factors,enzymes,and *** mechanisms of action of these molecules are discussed,considering traumatic spinal cord injury in rodents and humans.

关键词： axonal regeneration drugs enzymes growth factors molecular therapy neurotrophic factors purines spinal cord injury

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Therapeutic effectiveness of a single exercise session combined with WalkAide functional electrical stimulation in post-stroke patients:a crossover design study

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Neural Regeneration Research 2021年第5期16卷 805-812页

作者： Viviane Rostirola Elsner Lucieli Trevizol Isadora de Leon Marcos da Silva ThaynáWeiss Milena Braga Daniela Pochmann Amanda Stolzenberg Blembeel Caroline Dani Elenice Boggio Curso de Fisioterapia do centro Universitário Metodista-IPA Porto AlegreRSBrasil Programa de Pós-Graduação em Biociências e Reabilitação do centro Universitário Metodista-IPA Porto AlegreRSBrasil Programa de Pós-Graduação em ciências Biológicas:Fisiologia Universidade Federal do Rio Grande do SulPorto AlegreRSBrasil

A growing body of evidence has suggested that the imbalance of epigenetic markers and oxidative stress appears to be involved in the pathophysiology and progression of ***,strategies that modulate these biomarkers might be considered targets for neuroprotection and novel therapeutic opportunities for these *** exercise has been reported to induce changes in these epigenetic markers and improve clinical outcomes in different ***,little is reported on this in post-stroke *** purpose of this study was to investigate the effect of a single exercise session with WalkAide functional electrical stimulation(FES)on cognitive performance,clinical functional parameters,oxidative stress and epigenetic modulation in post-stroke *** this crossover design study,12 post-stroke individuals aged 54–72 years of either sexes were included and subjected to a single session of exercise(45 minutes)without WalkAide functional electrical stimulation(EXE alone group),followed by another single session of exercise(45 minutes)with WalkAide functional electrical stimulation(EXE+FES group).The clinical functional outcome measures,cognitive performance and blood collections for biomarker measurements were assessed pre-and *** intervention,higher Berg Balance Scale scores were obtained in the EXE+FES group than in the EXE alone *** was no significant difference in the Timed Up and Go test results post-intervention between EXE alone and EXE+FES *** intervention,a better cognitive performance was found in both groups compared with before the *** intervention,the Timed Up and Go test scores were higher in the EXE+FES group than in the EXE alone *** addition,the intervention induced lower levels of lipid *** intervention,carbonyl level was lower,superoxide dismutase activity and superoxide dismutase/catalase activity ratio were higher in the EXE+FES group,compared with the

关键词： cognition epigenetic exercise functional electrical stimulation functional mobility oxidative stress stroke WalkAide

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Genetic diversity and structure of Drimys brasiliensis in southern Brazil:insights for conservation

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Journal of Forestry Research 2020年第4期31卷 1325-1332页

作者： Alexandre Mariot Tiago Montagna Maurício Sedrez dos Reis Núcleo de Pesquisas em Florestas Tropicais Pós-Graduacāo Em Recursos Genéticos VegetaisCentro de Ciências AgráriasUniversidade Federal de Santa CatarinaRodovia Admar Gonzaga1346FlorianópolisSC 88034-000Brazil

Population genetics studies are widely recognized for generating useful knowledge for biodiversity *** date,however,little is known about the levels and distribution of genetic diversity of Drymis brasiliensis(Miers LC),a tree species from the Atlantic ***,in this study,we investigated how genetic diversity is distributed within and among populations of *** from southern Brazil using allozyme markers to genotype reproductive trees(8 populations)and seedlings(3 populations).Furthermore,in two populations,we established two permanent plots(5.1 and 1 ha)to analyze fine-scale genetic structure(FSGS).Studied populations presented low levels of genetic diversity(reproductive=0.085;seedlings=0.054)and high fixation indexes(reproductive=0.396;seedlings=0.231).Genetic divergence among populations was equal to 0.05,which is significant,signaling that few populations can conserve large portions of the species total genetic *** was only detected for one population,when reproductive individuals were separated by less than40 *** genetic diversity combined with high fixation indexes clearly signal a risk of losing ***,conservation efforts should be aimed at enhancing gene flow within the studied populations.

关键词： Atlantic Rainforest Effective population size Fine-scale genetic structure Seed collection

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Growth and phenotypic plasticity of two tropical tree species under low light availability

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Journal of Plant Ecology 2021年第2期14卷 270-279页

作者： Marúcia da Cunha Fagundes Ândrea Carla Dalmolin Luciana Santos Lobo Ana Cristina Schilling Martielly Santana dos Santos Marcelo Schramm Mielke departamento de ciências Biológicas Universidade Estadual de Santa CruzIlhéusBABrazil centro de formação em ciências agroflorestais Universidade Federal do Sul da BahiaIlhéusBABrazil departamento de ciências Exatas e Tecnológicas Universidade Estadual de Santa CruzIlhéusBABrazil

Aims Screening tree species in tropical rainforest according to their shade tolerance is important to efficiently manage the native trees of economic significance in secondary forest enrichment *** objective of this study was to determine the whole-plant light compensation point(WPLCP)and compare the phenotypic plasticity in relation to growth and carbon allocation of Cariniana legalis and Gallesia integrifolia seedlings under low light *** Seedlings were cultivated for 77 days under conditions of five photosynthetically active radiation(PAR)(0.02,1.1,2.3,4.5 and 5.9 mol photons m^(−2)day^(−1))in three *** and carbon allocation variables were *** Findings Growth rates of *** were higher and lower than those of *** under 1.1 and 5.9 mol photons m^(−2)day^(−1),*** WPLCP differed significantly between the two *** accordance with the criteria of the shade tolerance classification for these two tropical tree species,our results showed that *** had lower WPLCP and phenotypic plasticity in terms of higher growth rates and greater shade tolerance than *** a practical point of view,we demonstrated that the differential linkage between growth and changing PAR between the two species can become a useful tool for comparing and selecting tree species in forest enrichment projects.

关键词： Cariniana legalis Gallesia integrifolia rainforest tropical tree seedlings relative distance plasticity index whole-plant light compensation point

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