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Silk-ELR co-recombinamer covered stents obtained by electrospinning

Regenerative Biomaterials 2019年第1期6卷 21-28页

作者： M.Putzu F.Causa Manuel Parente Israel Gonza´lez de Torre J.C.Rodriguez-Cabello P.A.Netti Dipartimento di Ingegneria Chimica dei Materiali e della Produzione Industriale(DICMAPI)University“Federico II”Piazzale Tecchio 8080125 NaplesItaly Interdisciplinary research centre on Biomaterials(CRIB) University of Naples Federico IIPiazzale Tecchio 8080125 NapoliItaly Center for advanced Biomaterials for Health Care@CRIB Istituto Italiano di TecnologiaLargo Barsanti e Matteucci 5380125 NapoliItaly Conic Vascular via Carlo Maderno 236901 LuganoSwitzerland Technical Proteins NanoBioTechnology S.L. Edificio CTTAPaseo de Belen9A47011 ValladolidSpain Networking research Center on Bioengineering Biomaterials and Nanomedicine(CIBER-BBN)ValladolidSpain Bioforge Lab University of ValladolidEdificio LUCIAPaseo de Belen1947011 ValladolidSpain

In the field of tissue engineering the choice of materials is of great importance given the possibility to use biocompatible polymers produced by means of biotechnology.A large number of synthetic and natural materials have been used to this purpose and processed into scaffolds using Electrospinning *** materials that could be used for the fabrication of scaffold and degradable membranes,natural polymers such as collagen,elastin or fibroin offer the possibility to design structures strictly similar to the extracellular matrix(ECM).Biotechnology and genetic engineering made possible the advent of a new class of biopolymers called protein-based *** example is represented by the silk-elastin-proteins that combine the elasticity and resilience of elastin with the high tensile strength of silk-fibroin and display engineered bioactive *** this work,we use electrospinning technique to produce a fibrous scaffold made of the corecombinamer *** fibres have been characterized from the morphological point of *** and morphology have been explored using Scanning Electron Microscopy.A thorough study regarding the influence of Voltage,flow rate and distance have been carried out to determine the appropriate parameters to obtain the fibrous mats without defects and with a good distribution of *** has also been in vitro *** the first time we use the co-recombinamer Silk-ELR for the fabrication of a 2.5 angioplasty balloon *** structure could be useful as a coated scaffold for the regeneration of intima layer of vessels.

关键词： electrospinning silk elastin-like-recombinamers tissue engineering

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Re-Thinking Biology—I. Maxwell’s Demon and the Spontaneous Origin of Life

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Advances in Biological Chemistry 2017年第5期7卷 170-181页

作者： Christopher Busby Charles Vyvyan Howard Environmental research SIA 1117 Latvian Academy of Sciences Riga Latvia centre for Molecular Biosciences University of Ulster Coleraine UK

A hypothesis is advanced in which life began from a Darwinian selection among a diversification of molecular species containing the phosphate moiety which broke the constraints implicit in the Second Law of Thermodynamics, discussed famously by Schrodinger, by obtaining energy from specific infrared frequencies located in the phosphorus-oxygen vibration at a frequency around 1000 cm-1. We propose the source of this energy was from the internal conversion of solar broadband energy by the phosphate mineral Apatite, present at the bottom of a primitive biogenesis pond. In this scenario, life is re-defined as being hotter than its environment and as using its excess energy, supplied by infra-red conversion, to react with its molecular environment and pump itself up the “entropy slope” thereby;replication is through breakages of increasingly large phosphate containing biopolymers. The idea has implications for modern explanations of living systems.

关键词： Abiogenesis Evolution Infra-Red Radiation Thermodynamics 2nd Law Maxwell’s Demon Phosphate

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Taste masking of ciprofloxacin by ion-exchange resin and sustain release at gastric-intestinal through interpenetrating polymer network

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Asian Journal of Pharmaceutical Sciences 2015年第4期10卷 331-340页

作者： A.Michael Rajesh Shreya A.Bhatt Harshad Brahmbhatt Pritpal Singh Anand Kiritkumar Mangaldas Popat Reverse Osmosis Membrane Division Central Salt and Marine Chemicals Research InstituteCouncil of Scientific&Industrial Research(CSIR)G.B.MargBhavnagar 364002India Analytical Discipline&Centralized Instrumentation Facility Central Salt and Marine Chemicals Research InstituteCouncil of Scientific&Industrial Research(CSIR)G.B.MargBhavnagar 364002India Academy of Scientific and Innovative research Central Salt and Marine Chemicals Research InstituteCouncil of Scientific&Industrial Research(CSIR)G.B.MargBhavnagar 364002India

The aim of the study was to taste mask ciprofloxacin(CP)by using ion-exchange resins(IERs)followed by sustain release of CP by forming interpenetrating polymer network(IPN).IERs based on the copolymerization of acrylic acid with different cross linking agents were ***-resin complexes(DRCs)with three different ratios of drug to IERs(1:1,1:2,1:4)were prepared&evaluated for taste masking by following in vivo and in vitro *** volunteers graded ADC 1:4,acrylic acid-divinyl benzene(ADC-3)resin as *** studies such as FTIR,SEM,DSC,P-XRD differentiated ADC 1:4,from physical mixture(PM 1:4)and confirmed the formation of *** vitro drug release of ADC 1:4 showed complete release of CP within 60 min at simulated gastric fluid(SGF)*** *** beads were prepared with ADC 1:4 by using sodium alginate(AL)and sodium alginate-chitosan(AL-CS)for sustain release of CP at SGF pH and followed by simulated intestinal fluid(SIF *** 7.4).FTIR spectra confirmed the formation of IPN *** release of CP was sustain at SGF pH(75%).The kinetic model of IPN beads showed the release of CP was non-Fickian diffusion type.

关键词： Ion exchange resins biopolymers In vitro&in vivo taste masking Ciprofloxacin Sustain release Release mechanism and kinetics

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Extrusion of Thermoplastic Starch: Effect of “Green” and Common Polyethylene on the Hydrophobicity Characteristics

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Materials Sciences and Applications 2014年第12期5卷 845-856页

作者： Muhammad Pervaiz Philip Oakley Mohini Sain centre for Biocomposites and Biomaterials Processing Faculty of Forestry University of Toronto Toronto Canada GreenCore Composites Inc. Sarnia Canada The Delphi Group Ottawa Canada centre of advanced Chemistry King Abdulaziz University Jeddah KSA Mechanical Engineering Department Lule&aring University of Technology Lule&aring Sweden

Novel plastics that are biodegradable, environmentally benign, and made from renewable natural resources are currently being researched as alternatives to traditional petroleum-based plastics. One such plastic, thermoplastic starch (TPS) is produced from starch processed at high temperatures in the presence of plasticizers, such as water and glycerol. However, because of its hydrophilic nature, TPS exhibits poor mechanical properties when exposed to environmental conditions, such as rain or humidity. The overall objective of this research work was to produce a thermoplastic starch based material with low water absorption that may be used to replace petroleum-based plastics. With a recent emergence of “green” polyethylene (GPE), sourced from renewable feedstock, it has become possible to develop novel biodegradable polymers for various applications. In this work, GPE was melt blended with starch in three different ways;reactive extrusion of GPE and starch facilitated by maleic anhydride (MAH) and dicumyl peroxide (DCP), melt blending of GPE and starch by extrusion, and melt blending of maleated polyethylene and starch by extrusion. Comprehensive testing and analysis has shown that all methods reduced water absorption significantly with some variations across the board.

关键词： biopolymers Thermoplastic Starch Biodegradable Green Polyethylene Hydrophobic Water Absorption Melt-Blending of Starch Renewable Resources

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An integrated theoretical and experimental investigation of insensitive munition compounds adsorption on cellulose,cellulose triacetate, chitin and chitosan surfaces

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Journal of Environmental Sciences 2018年第2期30卷 174-180页

作者： Luke A.Gurtowski Chris S.Griggs Veera G.Gude Manoj K.Shukla Environmental Laboratory Engineer Research and Development Center Department of Civil and Environmental Engineering Mississippi State University

This manuscript reports results of combined computational chemistry and batch adsorption investigation of insensitive munition compounds, 2,4-dinitroanisole（DNAN）, triaminotrinitrobenzene（TATB）, 1,1-diamino-2,2-dinitroethene（FOX-7） and nitroguanidine（NQ）, and traditional munition compound 2,4,6-trinitrotoluene（TNT） on the surfaces of cellulose, cellulose triacetate, chitin and chitosan biopolymers. Cellulose,cellulose triacetate, chitin and chitosan were modeled as trimeric form of the linear chain of4 C1 chair conformation of β-D-glucopyranos, its triacetate form, β-N-acetylglucosamine and D-glucosamine, respectively, in the 1 ？ 4 linkage. Geometries were optimized at the M062 X functional level of the density functional theory（DFT） using the 6-31 G（d,p） basis set in the gas phase and in the bulk water solution using the conductor-like polarizable continuum model（CPCM） approach. The nature of potential energy surfaces of the optimized geometries were ascertained through the harmonic vibrational frequency analysis. The basis set superposition error（BSSE） corrected interaction energies were obtained using the 6-311 G（d,p）basis set at the same theoretical level. The computed BSSE in the gas phase was used to correct interaction energy in the bulk water solution. Computed and experimental results regarding the ability of considered surfaces in adsorbing the insensitive munitions compounds are discussed.

关键词： Cellulose Cellulose triacetate Chitin Chitosan 2,4-Dinitroanisole(DNAN) Triaminotrinitrobenzene(TATB) 1,1-Diamino-2,2-dinitroethene(FOX-7) Nitroguanidine(NQ)

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Single-cell omics analyses with single molecular detection:challenges and perspectives

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The Journal of Biomedical research 2021年第4期35卷 264-276页

作者： Gradimir Misevic research and Development Gimmune GmbH6302 ZugSwitzerland

The ultimate goal of single-cell analyses is to obtain the biomolecular content for each cell in unicellular and multicellular organisms at different points of their life cycle under variable environmental *** require an assessment of:a)the total number of cells,b)the total number of cell types,and c)the complete and quantitative single molecular detection and identification for all classes of biopolymers,and organic and inorganic compounds,in each individual *** proteins,glycans,lipids,and metabolites,whose sequences cannot be amplified by copying as in the case of nucleic acids,the detection limit by mass spectrometry is about 105 ***,proteomic,glycomic,lipidomic,and metabolomic analyses do not yet permit the assembly of the complete single-cell *** construction of novel nanoelectrophoretic arrays and nano in microarrays on a single 1-cm-diameter chip has shown proof of concept for a high throughput platform for parallel processing of thousands of individual *** with dynamic secondary ion mass spectrometry,with 3D scanning capability and lateral resolution of 50 nm,the sensitivity of single molecular quantification and identification for all classes of biomolecules could be *** development and routine application of such technological and instrumentation solution would allow assembly of complete omes with a quantitative assessment of structural and functional cellular diversity at the molecular level.

关键词： single-cell analyses single molecular detection ome transcriptomics proteomics glycomics lipidomics metabolomics

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Characterization of changes in extracellular polymeric substances and heavy metal speciation of waste activated sludge during typical oxidation solubilization processes

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Journal of Environmental Sciences 2019年第6期31卷 146-158页

作者： Qiongying Xu Huidi Wang Qiandi Wang Weijun Zhang Dongsheng Wang Faculty of Materials Science and Chemistry China University of Geosciences School of Environmental Studies China University of Geosciences Hubei Provincial Engineering research Center of Systematic Water Pollution Control State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences

Biopolymer solubilization is considered to be the rate-limiting stage of anaerobic digestion of waste activated sludge(WAS). Oxidation processes have been proven to be effective in disrupting sludge flocs and causing solubilization of the solid biopolymers. In this study,WAS was treated by NaNO2 or H2 O2 oxidation at p H of 2. The changes in extracellular polymeric substances properties and the speciation of heavy metals were investigated. The results revealed that both NaNO2 and H2 O2 treatments were effective in solubilizing organics in WAS, while the conversion of biopolymers in the two treatment processes was different. Free nitrous acid destroyed the gel network structure of EPS, and organic materials were released from the solid phase to the supernatant. Indigenous peroxidase catalyzed H2 O2 to produce hydroxyl radicals which caused significant solubilization of biopolymers, and the protein-like substances were further degraded into micro-molecule polypeptides or amino acids at high dosages of H2 O2. During the oxidation processes, Zn, Cd and Cu, with excellent mobility, tended to migrate to the supernatant, and thus were easy to remove through the liquid–solid separation process. Ni and As showed moderate migration ability, of which the residual fraction tended to transform into reducible and soluble fractions. With poor mobility, Cr and Pb mainly existed in the forms of residual and oxidizable fractions, which were difficult to dissolve and remove from WAS. Both NaNO2 and H2 O2 treatment resulted in the enhancement of sludge solubilization efficiency and heavy metal mobility in WAS, but different heavy metals showed distinct migration and transformation behaviors.

关键词： Extracellular polymeric substances(EPS) Oxidation biopolymers solubilization Biodegradability Heavy metals

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biopolymers and Biomimetic Materials in Medical and Electronic-Related Applications for Environment-Health-Development Nexus:Systematic Review

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Journal of Bionic Engineering 2022年第6期19卷 1562-1577页

作者： Faris Mohammed AL-Oqla Mohammed Hassouna Alaaeddin Muhammad Enamul Hoque Vijay Kumar Thakur Department of Mechanical Engineering Faculty of EngineeringThe Hashemite UniversityP.O Box 330127Zarqa13133Jordan University College Fairview 2AJalan TP2Sime UEP Industrial Park Subang Jaya47600Subang JayaSelangorMalaysia Department of Biomedical Engineering Military Institute of Science and Technology(MIST)Mirpur CantonmentDhaka1216Bangladesh Biorefining and advanced Materials research Center Scotland’s Rural College(SRUC)Kings BuildingsEdinburghEH93JGUK School of Engineering University of Petroleum&Energy Studies(UPES)Dehradun248007UttarakhandIndia

Biocomposites as bio-inspired materials are produced from renewable resources that are organic and ecofriendly alternative *** improve the lifestyle of human beings as well as enhancing the environmental indices,functional bio-materials are now implemented in various promising *** work has systematically discussed and highlighted the implementations and trends of functional bionic materials in high tech industries,which are necessary for developing modern *** medical,electronic,food and pharmaceutical applications have been ***-inspired materials are used to develop more sustainable possibilities to increase environmental conservation while maintaining customer *** were found employed in several sectors for various functional bio-products including organic thin-film transistors,organic phototransistor,emitting diodes,photodiodes,photovoltaic solar cells,hybrid dental resins,sustainable pharmaceuticals,and food *** are used to create sustainable bio-products for energy storage and harvesting,bone regeneration,nerve damage repair,drug applications and various other industrial subcategories.

关键词： biopolymers Bioinspired Biomimetic materials Tissue engineering Nerve repair Organic transistors

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Utilization of Bitter Orange Seed as a Novel Pectin Source:Compositional and Rheological Characterization

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Journal of Renewable Materials 2022年第11期10卷 2805-2817页

作者： Diako Khodaei Mohammad Nejatian Hassan Ahmadi Gavlighi Farhad Garavand Ilaria Cacciotti Department of Sport Exercise and NutritionAtlantic Technological UniversityATU Galway CityGalwayH91 T8NWIreland Department of Food Science and Technology Tarbiat Modares UniversityTehran14115-336Iran Department of Food Chemistry and Technology Teagasc Moorepark Food Research CentreFermoyCo.CorkP61 C996Ireland Department of Engineering INSTM RUUniversity of Rome‘NiccolòCusano’Rome00166Italy

The seeds from bitter orange,the by-product of juice making units,hold the potential to facilitate novel,easy yet high-quality pectin *** test this hypothesis,orange seed pectin(OSP)was extracted by distilled water and its compositional parameters and rheological behavior were then *** showed that galacturonic acid was the major component of OSP(∼425 mg/g)confirming the purity of the extracted pectin,followed by glucose and some minor neutral *** Mw(weight-average molar mass),Rn(number average molar mass),and Rz(z-average molar mass)values for the OSP were 4511.8 kDa,61 nm,and 61.1 nm,*** measurements showed a shear-thinning behavior for OSP so that the viscosity of the gum decreased by increasing the temperature from 5 to 45℃.The Power-law model fitted as the best rheological model describing the flow behavior of *** strain sweep dynamic rheological measurements confirmed an entangled network structure for OSP and the NaCl addition to the gum dispersion decreased the consistency coefficient from 35.6 to 23.18 ***,while the flow behavior index remained *** results demonstrate that the OSP can be used as a new source of pectin,with likely a wide range of applications in the food industry.

关键词： biopolymers waste management sugar composition molecular weight rheological models

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Extraction and Characterization of Litopenaeus vannamei’s Shell as Potential Sources of Chitosan biopolymers

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Journal of Renewable Materials 2023年第3期11卷 1181-1197页

作者： Che Engku Noramalina Che Engku Chik Amyra Suryatie Kamaruzzan Ahmad Ideris Abdul Rahim Fathurrahman Lananan Azizah Endut Siti Aslamyah Nor Azman Kasan Higher Institution centre of Excellence(HICoE) Institute of Tropical Aquaculture and Fisheries(AKUATROP)Universiti MalaysiaTerengganuMalaysia East Coast Environmental research Institute(ESERI) Universiti Sultan Zainal Abidin(UniSZA)Terengganu Darul ImanMalaysia Faculty of Bioresources and Food Industry Universiti Sultan Zainal Abidin(UniSZA)Terengganu Darul ImanMalaysia Faculty of Innovative Design and Technology Universiti Sultan Zainal AbidinTerengganu Darul ImanMalaysia Faculty of Marine Science and Fisheries Hasanuddin UniversityMakassarIndonesia

Chitin is the second most abundant polysaccharide,produced mainly as an industrial waste stream during crustacean *** can be derived into chitosan through the deacetylation *** of shrimp waste into chitosan via the deacetylation process could be considered a practical approach for shell waste *** this study,chitosan’s physicochemical characteristics extracted from two types of Pacific white leg shrimp,***’s shell(i.e.,rough and smooth),were compared with commercial *** yield,moisture,ash,solubility,water and fat binding capacity were *** degree of deacetylation(DDA)was calculated using FTIR,and their chemical Structure was confirmed using XRD and *** extracted chitosan showed no significant difference in yield,moisture,ash,solubility and water binding capacity but showed a significant difference with commercial ***,the fat binding capacity of commercial chitosan showed the lowest percentage(408.34±0.83%)as compared to extracted chitosan(smooth shell 549.59±12.48%;rough shell 500.55±12.10%).The DDA indicated that extracted chitosan from the smooth and rough shell was considered good chitosan as compared to commercial chitosan with 84.08±1.27%,80.78±0.79%and 74.99±1.48%,***,the presence of hydroxyl and amino groups from FTIR and a good crystallinity index was recorded using XRD of extracted *** on observed characteristics,shrimp shell waste from *** can achieve chitosan standard quality as a biopolymer and highly potential to be applied in various industrial applications.

关键词： Chitin chitosan degree of deacetylation L.vannamei shrimp shell surface morphology

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