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Deformable microlaser force sensing

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Light(Science & Applications) 2024年第6期13卷 1196-1209页

作者： Eleni Dalaka Joseph SHill Jonathan H.H.Booth Anna Popczyk Stefan R.Pulver Malte C.Gather Marcel Schubert centre of biophotonics SUPASchool of Physics and AstronomyUniversity of St AndrewsNorth HaughSt AndrewsUK Humboldt centre for Nano-and biophotonics Department of ChemistryUniversity of CologneKölnGermany School of Psychology and Neuroscience University of St AndrewsSt Mary’s QuadSouth StreetSt AndrewsUK Institute for Bioengineering of Catalonia BarcelonaSpain

Mechanical forces are key regulators of cellular behavior and function,affecting many fundamental biological processes such as cell migration,embryogenesis,immunological responses,and pathological *** force sensors and imaging techniques have been developed to quantify these otherwise invisible forces in single cells and in ***,current techniques rely heavily on high-resolution microscopy and do not allow interrogation of optically dense tissue,reducing their application to 2D cell cultures and highly transparent biological ***,we introduce DEFORM,deformable microlaser force sensing,a spectroscopic technique that detects sub-nanonewton forces with unprecedented spatio-temporal *** is based on the spectral analysis of laser emission from dye-doped oil microdroplets and uses the force-induced lifting of laser mode degeneracy in these droplets to detect nanometer *** validation by atomic force microscopy and development of a model that links changes in laser spectrum to applied force,DEFORM is used to measure forces in 3D and at depths of hundreds of microns within tumor spheroids and late-stage Drosophila *** furthermore show continuous force sensing with single-cell spatial and millisecond temporal resolution,thus paving the way for non-invasive studies of biomechanical forces in advanced stages of embryogenesis,tissue remodeling,and tumor invasion.

关键词： invasion otherwise hundreds

Light sheet microscope scanning of biointegrated microlasers for localized refractive index sensing

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Photonics Research 2024年第8期12卷 1673-1680页

作者： ROSS C.COWIE MARCEL SCHUBERT SUPA School of Physics and AstronomyUniversity of St Andrews Humboldt centre for Nano and biophotonics Department of ChemistryUniversity of Cologne

Whispering gallery mode (WGM) microlasers are highly sensitive to localized refractive index changes allowing to link their emission spectrum to various chemical, mechanical, or physical stimuli. Microlasers recently found applications in biological studies within single cells, in three-dimensional samples such as multicellular spheroids,or in vivo. However, detailed studies of biological samples also need to account for the structural heterogeneity of tissues and live animals, therefore requiring a combination of high-resolution microscopy and laser ***,we design and construct a light sheet fluorescence microscope with a coupled spectrometer for use in microlaser studies for combined high-resolution, high-speed imaging and WGM spectral analysis. The light sheet illumination profile and the decoupled geometry of excitation and emission hereby directly affect the lasing and sensing properties, mainly through geometric constraints and by light coupling effects. We demonstrate the basic working principle of microlaser spectroscopy under light sheet excitation and measure the absolute refractive index within agarose and in zebrafish tail muscle tissue. We further analyze the light coupling conditions that lead to the occurrence of two separate oscillation planes. These so-called cross modes can be scanned around the entire microlaser surface, which allows to estimate a surface-averaged refractive index profile of the microlaser environment.

关键词： High speed imaging Light sheet fluorescence microscopy Refractive index Scanning microscopy Second harmonic generation Solid state lasers

Light-deformable microrobots shape up for the biological obstacle course

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Light(Science & Applications) 2024年第5期13卷 803-805页

作者： Philip Wijesinghe centre of biophotonics SUPASchool of Physics and AstronomyUniversity of St AndrewsNorth HaughSt AndrewsFife KY169SSUK

Euglena gracilis microalga has been transformed into a soft bio-microrobot with light-controlled motion and deformation that can address diverse bio-challenges,such as drug delivery,diseased cell removal,and photodyna... 详细信息

关键词： Euglena transformed obstacle

Active digital spoof plasmonics

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National Science Review 2020年第2期7卷 261-269页

作者： Hao Chi Zhang Tie Jun Cui Yu Luo Jingjing Zhang Jie Xu Pei Hang He Le Peng Zhang State Key Laboratory of Millimeter Waves Southeast University The Photonics Institute and centre for optoelectronics and biophotonics School of Electrical and Electronic Engineering Nanyang Technological University

Digital coding and digital modulation are the foundation of modern information science. The combination of digital technology with metamaterials provides a powerful scheme for spatial and temporal controls of electromagnetic waves. Such a technique, however, has thus far been limited to the control of free-space light. Its application to plasmonics to shape subwavelength fields still remains elusive. Here, we report the design and experimental realization of a tunable conformal plasmonic metasurface, which is capable of digitally coding and modulating designer surface plasmons at the deep-subwavelength scale. Based on dynamical switching between two discrete dispersion states in a controlled manner, we achieve digital modulations of both amplitude and phase of surface waves with nearly 100% modulation depth on a single device. Our study not only introduces a new approach for active dispersion engineering, but also constitutes an important step towards the realization of subwavelength integrated plasmonic circuits.

关键词： digital coding digital modulation plasmonics digital metamaterials

Photoluminescence of SiV centers in CVD diamond particles with specific crystallographic planes

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Chinese Physics B 2019年第1期28卷 470-477页

作者： Ying-Shuang Mei Cheng-Ke Chen Mei-Yan Jiang Xiao Li Yin-Lan Ruan Xiao-Jun Hu College of Materials Science and Engineering Zhejiang University of Technology ARC centre of Excellence in Nanoscale biophotonics Institute of Photonics and Advanced Sensing University of Adelaide

We prepared the isolated micrometer-sized diamond particles without seeding on the substrate in hot filament chemical vapor deposition. The diamond particles with specific crystallographic planes and strong silicon-vacancy(SiV) photoluminescence(PL) have been prepared by adjusting the growth pressure. As the growth pressure increases from 2.5 to 3.5 kPa,the diamond particles transit from composite planes of {100} and {111} to only smooth {111} planes. The {111}-faceted diamond particles present better crystal quality and stronger normalized intensity of SiV PL with a narrower bandwidth of 5 nm. Raman depth profiles show that the SiV centers are more likely to be formed on the near-surface areas of the diamond particles, which have poorer crystal quality and greater lattice stress than the inner areas. Complex lattice stress environment in the near-surface areas broadens the bandwidth of SiV PL peak. These results provide a feasible method to prepare diamond particles with specific crystallographic planes and stronger SiV PL.

关键词： diamond particle SiV center photoluminescence crystallographic planes

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A Non-Singular Boundary Element Method for Interactions between Acoustical Field Sources and Structures

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Advances in Applied Mathematics and Mechanics 2023年第4期15卷 831-851页

作者： Qiang Sun Australian Research Council centre of Excellence for Nanoscale biophotonics School of ScienceRMIT UniversityMelbourneVIC 3001Australia

Localized point sources(monopoles)in an acoustical domain are implemented to a three dimensional non-singular Helmholtz boundary element method in the frequency *** allows for the straightforward use of higher order surface elements on the boundaries of the *** will been shown that the effect of the monopole sources ends up on the right hand side of the resulting matrix *** carefully selected examples are studied,such as point sources near and within a concentric spherical core-shell scatterer(with theoretical verification),near a curved focusing surface and near a multi-scale and multi-domain acoustic lens.

关键词： Acoustic monopoles acoustic lens boundary integral method quadratic elements

High Q-factor controllable phononic modes in hybrid phononic–dielectric structures

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Advanced Photonics 2019年第2期1卷 41-45页

作者： Bo Qiang Alexander M.Dubrovkin Harish N.S.Krishnamoorthy Qian Wang Cesare Soci Ying Zhang Jinghua Teng Qi Jie Wang Nanyang Technological University Centre for Disruptive Photonic TechnologiesThe Photonic InstituteSchool of Physical and Mathematical SciencesSingapore Nanyang Technological University Centre for OptoElectronics and BiophotonicsSchool of Electrical and Electronic EngineeringSingapore Agency for Science Technology and ResearchInstitute of Materials Research and EngineeringSingapore Agency for Science Technology and ResearchInstitute of Manufacturing TechnologySingapore

Phonon polariton resonances in the mid-infrared spectral range demonstrate properties superior to noble metal-based plasmonics,owing to smaller dissipative loss and better field ***,a conventional way to excite the localized phonon resonance involves ion etching,which reduces the attainable quality factors(Q-factors)of the *** show that by introducing a deep subwavelength layer of dielectric gratings on a phononic substrate,localized dipolar resonance and higher order modes with high Q-factors 96 and 195,respectively,can be *** further demonstrate,via experiments and simulations,that the resonant wavelength and field confinement can be controlled by coupling the localized hybrid mode with propagating surface *** also observed for the first time the coupling between a localized dipolar mode and a propagating higher-order surface phonon-polariton *** results will be useful in designing on-chip,low-loss,and highly integrated phononic devices in the infrared spectral domain.

关键词： hybrid resonance high Q-factor phonon resonance mode splitting

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Synthesis and characterization of reporter molecules embedded core-shell nanoparticles as SERS nanotags

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Journal of Innovative Optical Health Sciences 2021年第4期4卷 94-103页

作者： Chloe Duffield Nana Lyu Yuling Wang Department of Molecular Sciences ARC centre of Excellence for Nanoscale biophotonics Faculty of Science and Engineering Macquarie University SydneyNSW 2109Australia

Surface-enhanced Raman scattering(SERS)spectroscopy is presented as a sensitive and spe-cific molecular tool for clinical diagnosis and prognosis monitoring of various diseases including *** order for clinical application of SERS technique,an ideal method of bulk synthesis of SERS nanoparticles is necessary to obtain sensitive,stable and highly reproducible Raman *** this contribution,we determined the ideal conditions for bulk synthesis of Raman reporter(Ra)molecules embedded silver-gold core-shell nanoparticles(Au@Ra@AgNPs)using hydroquinone as reducing agent of silver *** using UV-Vis spectroscopy,Raman spectroscopy and transmission electron microscopy(TEM),we found that a 2∶1 ratio of silver nitrate to hydroquinone is ideal for a uniform silver coating with a strong and stable Raman *** stability testing of the optimized Au@Ra@AgNPs over a two-week period,these SERS nanotags were found to be stable with minimal signal change *** sta-bility of antibody linked SERS nanotags is also crucial for cancer and disease diagnosis,thus,we further conjugated the as-prepared SERS nanotags with anti-EpCAM antibody,in which the stability of bioconjugated SERS nanotags was tested over eight *** UV-Vis and SERS spectroscopy showed stable absorption and Raman signals on the anti-EpCAM conju-gated SERS nanotags,indicating the great potential of the synthesized SERS nanotags for future applications which require large,reproducible and uniform quantities in order for cancer biomarker diagnosis and monitoring.

关键词： surface-enhanced Raman spectroscopy gold nanoparticles Raman reporter mole-cules SERS nanotags and bioconjugation

Synthesis of strong SiV photoluminescent diamond particles on silica optical fiber by chemical vapor deposition

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Chinese Physics B 2018年第3期27卷 481-486页

作者： Zongchun Yang Yingshuang Mei Chengke Chen Yinlan Ruan Xiaojun Hu College of Materials Science and Engineering Zhejiang University of Technology Hangzhou 310014 China ARC centre of Excellence in Nanoscale biophotonics Institute of Photonics and Advanced Sensing The University of Adelaide Adelaide SA 5005 Australi

The separated silicon-vacancy （SiV） photoluminescent diamond particles were synthesized on a silica optical fiber by hot filament chemical vapor deposition （HFCVD）. The effects of the pre-treated method and chamber pressure on the microstructure and photoluminescence of the diamond particles were investigated. The results show that the diamond particles are homogeneously distributed on the surface of the optical fiber. With the chamber pressure increasing from 1.6 kPa to 3.5 kPa, the shape of the particles transforms from flake to circle, while the diamond particles cannot be deposited on the fiber with the pressure further increased to 4.5 kPa. The samples synthesized under 2.5 kPa chamber pressure are composed of diamond particles with size around 200-400 nm, exhibiting stronger SiV photoluminescence with the width of around 6 nm.

关键词： diamond silicon vacancy photoluminescence optical fiber

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